AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048302.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048302.01.T01 MTR_8g089340 92.035 226 18 0 1 226 236 461 4.38e-132 380
MsG0080048302.01.T01 MTR_8g089320 90.090 222 21 1 1 222 384 604 1.06e-131 384
MsG0080048302.01.T01 MTR_8g089360 67.453 212 64 3 1 212 372 578 1.38e-94 288
MsG0080048302.01.T01 MTR_8g089360 67.453 212 64 3 1 212 389 595 2.22e-94 288
MsG0080048302.01.T01 MTR_1g030660 62.564 195 71 1 1 195 379 571 7.48e-80 250
MsG0080048302.01.T01 MTR_2g035430 52.683 205 96 1 1 205 384 587 3.83e-72 230
MsG0080048302.01.T01 MTR_6g034535 46.078 204 104 3 1 200 112 313 5.92e-60 191
MsG0080048302.01.T01 MTR_8g461380 47.449 196 100 3 1 195 391 584 5.49e-57 190
MsG0080048302.01.T01 MTR_1g111740 36.842 209 123 4 1 206 371 573 1.02e-35 132
MsG0080048302.01.T01 MTR_4g099270 36.364 198 114 4 1 192 226 417 3.73e-27 108
MsG0080048302.01.T01 MTR_4g099270 36.364 198 114 4 1 192 338 529 6.55e-27 108
MsG0080048302.01.T01 MTR_2g064760 86.207 29 4 0 107 135 13 41 5.46e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048302.01.T01 AT4G21120 57.207 222 84 2 1 222 232 442 6.86e-82 251
MsG0080048302.01.T01 AT4G21120 57.207 222 84 2 1 222 382 592 3.28e-80 251
MsG0080048302.01.T01 AT2G34960 52.381 189 90 0 1 189 375 563 9.63e-66 213
MsG0080048302.01.T01 AT1G17120 52.381 189 88 2 1 189 393 579 4.20e-57 191
MsG0080048302.01.T01 AT3G10600 40.704 199 97 5 1 189 232 419 4.98e-37 134
MsG0080048302.01.T01 AT3G10600 40.704 199 97 5 1 189 378 565 2.12e-36 134
MsG0080048302.01.T01 AT5G04770 39.894 188 106 3 1 185 232 415 3.23e-34 127
MsG0080048302.01.T01 AT5G04770 39.894 188 106 3 1 185 379 562 1.58e-33 127
MsG0080048302.01.T01 AT5G36940 44.444 63 35 0 1 63 348 410 8.16e-12 64.7

Find 51 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTGGAATTTCAGCTTAT+TGG 0.122057 contig26end:+27456 MsG0080048302.01.T01:CDS
GTGTGTCTTGTGTTGATTAT+TGG 0.202436 contig26end:+27428 MsG0080048302.01.T01:CDS
ATACATATCAAGTTATTAAT+TGG 0.204469 contig26end:-27867 None:intergenic
TTGGTATACTATGTGCTATT+AGG 0.217718 contig26end:+27707 MsG0080048302.01.T01:CDS
GCTATTAACATATTCCTTCT+TGG 0.225477 contig26end:+27632 MsG0080048302.01.T01:CDS
AATTGGAATTTCAGCTTATT+GGG 0.231227 contig26end:+27457 MsG0080048302.01.T01:CDS
TTGCTCCTTTGTGGTTTATA+GGG 0.241128 contig26end:+27519 MsG0080048302.01.T01:CDS
TTTGCTCCTTTGTGGTTTAT+AGG 0.244832 contig26end:+27518 MsG0080048302.01.T01:CDS
CTAATAGTAGTGAGCATAAT+TGG 0.262172 contig26end:+27480 MsG0080048302.01.T01:CDS
CGTATATAAGGTTTACGATT+TGG 0.279431 contig26end:+27672 MsG0080048302.01.T01:CDS
GGGGAGTTCCTTTGGTTCCT+TGG 0.281218 contig26end:+27591 MsG0080048302.01.T01:CDS
TTATAGGGACTGGTGCTCTT+TGG 0.283313 contig26end:+27534 MsG0080048302.01.T01:CDS
TGGTATACTATGTGCTATTA+GGG 0.320967 contig26end:+27708 MsG0080048302.01.T01:CDS
TGGCAAAGAAGCCTAAATTA+TGG 0.332142 contig26end:+27570 MsG0080048302.01.T01:CDS
AAAGGAACTCCCCATAATTT+AGG 0.346807 contig26end:-27581 None:intergenic
TAAATTATGGGGAGTTCCTT+TGG 0.356075 contig26end:+27583 MsG0080048302.01.T01:CDS
TTGATTATTGGTTCTTCAAT+TGG 0.371036 contig26end:+27440 MsG0080048302.01.T01:CDS
ATACAATCTTTGCTCCTTTG+TGG 0.380411 contig26end:+27510 MsG0080048302.01.T01:CDS
TTCCATTGCTTCTCAACCTC+CGG 0.382100 contig26end:-27794 None:intergenic
GGCAAAGAAGCCTAAATTAT+GGG 0.393362 contig26end:+27571 MsG0080048302.01.T01:CDS
GCTCTTGTTGATGAAAGAAC+TGG 0.394168 contig26end:+27212 MsG0080048302.01.T01:CDS
ATTGAGATTGTAGCATTCAT+AGG 0.394274 contig26end:-27239 None:intergenic
TCAAGTGGGGTAACTACAAA+AGG 0.402045 contig26end:+27386 MsG0080048302.01.T01:CDS
AATTTAGGCTTCTTTGCCAT+TGG 0.418140 contig26end:-27566 None:intergenic
CCTTTGTGGTTTATAGGGAC+TGG 0.440792 contig26end:+27524 MsG0080048302.01.T01:CDS
ATAAGGTTTACGATTTGGAC+AGG 0.465281 contig26end:+27677 MsG0080048302.01.T01:CDS
ACATAGCAGTGGTTCTGATC+CGG 0.487510 contig26end:+27775 MsG0080048302.01.T01:CDS
AAGGAATATGTTAATAGCAA+TGG 0.490509 contig26end:-27627 None:intergenic
AGTAGTGAGCATAATTGGAT+TGG 0.492532 contig26end:+27485 MsG0080048302.01.T01:CDS
TAAGGAGATATTATTCAAGT+GGG 0.502977 contig26end:+27372 MsG0080048302.01.T01:CDS
GATTTGGACAGGGTTTCTCT+TGG 0.516461 contig26end:+27688 MsG0080048302.01.T01:CDS
ATAAAGTAGAAATCGACAAA+AGG 0.538029 contig26end:-27316 None:intergenic
TTAATAGCAATGGATAAAGA+AGG 0.556623 contig26end:-27617 None:intergenic
ATCCGGAGGTTGAGAAGCAA+TGG 0.560522 contig26end:+27792 MsG0080048302.01.T01:CDS
CAAGTGGGGTAACTACAAAA+GGG 0.569446 contig26end:+27387 MsG0080048302.01.T01:CDS
GTAAGGAGATATTATTCAAG+TGG 0.577397 contig26end:+27371 MsG0080048302.01.T01:CDS
GTTAGCTAGAATACTAAGAT+CGG 0.584642 contig26end:-27294 None:intergenic
TGAGAAGCAATGGAACAAGA+TGG 0.601081 contig26end:+27802 MsG0080048302.01.T01:CDS
GTACTCACATGATGCCACCT+TGG 0.601374 contig26end:+27186 None:intergenic
GAAGGTAGCCAAGGAACCAA+AGG 0.606519 contig26end:-27599 None:intergenic
ATGGATAAAGAAGGTAGCCA+AGG 0.614956 contig26end:-27608 None:intergenic
TAAGGTTTACGATTTGGACA+GGG 0.615168 contig26end:+27678 MsG0080048302.01.T01:CDS
GATAAGAGTAAACATAGCAG+TGG 0.622480 contig26end:+27764 MsG0080048302.01.T01:CDS
CAATGGAACAAGATGGAGCA+AGG 0.635963 contig26end:+27809 MsG0080048302.01.T01:CDS
GCAAAGAAGCCTAAATTATG+GGG 0.643860 contig26end:+27572 MsG0080048302.01.T01:CDS
TAGCAGTGGTTCTGATCCGG+AGG 0.648811 contig26end:+27778 MsG0080048302.01.T01:CDS
AAGGAGATATTATTCAAGTG+GGG 0.676082 contig26end:+27373 MsG0080048302.01.T01:CDS
GTTACATGCTTCATATGACA+CGG 0.676963 contig26end:+27730 MsG0080048302.01.T01:CDS
CCAGTCCCTATAAACCACAA+AGG 0.715990 contig26end:-27524 None:intergenic
TCAACAAGAGCAAACCAAGG+TGG 0.728106 contig26end:-27200 None:intergenic
TCATCAACAAGAGCAAACCA+AGG 0.735762 contig26end:-27203 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATAAAAAATCGTATATA+AGG + contig26end:27660-27679 MsG0080048302.01.T01:CDS 15.0%
!!! TTTATTTATCTTTTCACTTG+TGG + contig26end:27334-27353 MsG0080048302.01.T01:CDS 20.0%
! AAGGAATATGTTAATAGCAA+TGG - contig26end:27630-27649 None:intergenic 25.0%
! AATTGGAATTTCAGCTTATT+GGG + contig26end:27457-27476 MsG0080048302.01.T01:CDS 25.0%
! ATAAAGTAGAAATCGACAAA+AGG - contig26end:27319-27338 None:intergenic 25.0%
! TAAGGAGATATTATTCAAGT+GGG + contig26end:27372-27391 MsG0080048302.01.T01:CDS 25.0%
! TTAATAGCAATGGATAAAGA+AGG - contig26end:27620-27639 None:intergenic 25.0%
!! TTTTATCAATAGTTCCAAGA+AGG - contig26end:27649-27668 None:intergenic 25.0%
!!! TTGATTATTGGTTCTTCAAT+TGG + contig26end:27440-27459 MsG0080048302.01.T01:CDS 25.0%
AAGGAGATATTATTCAAGTG+GGG + contig26end:27373-27392 MsG0080048302.01.T01:CDS 30.0%
ATTGAGATTGTAGCATTCAT+AGG - contig26end:27242-27261 None:intergenic 30.0%
CAATTGGAATTTCAGCTTAT+TGG + contig26end:27456-27475 MsG0080048302.01.T01:CDS 30.0%
CGTATATAAGGTTTACGATT+TGG + contig26end:27672-27691 MsG0080048302.01.T01:CDS 30.0%
CTAATAGTAGTGAGCATAAT+TGG + contig26end:27480-27499 MsG0080048302.01.T01:CDS 30.0%
GCTATTAACATATTCCTTCT+TGG + contig26end:27632-27651 MsG0080048302.01.T01:CDS 30.0%
GTAAGGAGATATTATTCAAG+TGG + contig26end:27371-27390 MsG0080048302.01.T01:CDS 30.0%
GTTAGCTAGAATACTAAGAT+CGG - contig26end:27297-27316 None:intergenic 30.0%
! TGGTATACTATGTGCTATTA+GGG + contig26end:27708-27727 MsG0080048302.01.T01:CDS 30.0%
! TTGGTATACTATGTGCTATT+AGG + contig26end:27707-27726 MsG0080048302.01.T01:CDS 30.0%
AAAGGAACTCCCCATAATTT+AGG - contig26end:27584-27603 None:intergenic 35.0%
AATTTAGGCTTCTTTGCCAT+TGG - contig26end:27569-27588 None:intergenic 35.0%
AGTAGTGAGCATAATTGGAT+TGG + contig26end:27485-27504 MsG0080048302.01.T01:CDS 35.0%
ATACAATCTTTGCTCCTTTG+TGG + contig26end:27510-27529 MsG0080048302.01.T01:CDS 35.0%
GATAAGAGTAAACATAGCAG+TGG + contig26end:27764-27783 MsG0080048302.01.T01:CDS 35.0%
GCAAAGAAGCCTAAATTATG+GGG + contig26end:27572-27591 MsG0080048302.01.T01:CDS 35.0%
GGCAAAGAAGCCTAAATTAT+GGG + contig26end:27571-27590 MsG0080048302.01.T01:CDS 35.0%
GTTACATGCTTCATATGACA+CGG + contig26end:27730-27749 MsG0080048302.01.T01:CDS 35.0%
TAAATTATGGGGAGTTCCTT+TGG + contig26end:27583-27602 MsG0080048302.01.T01:CDS 35.0%
TGGCAAAGAAGCCTAAATTA+TGG + contig26end:27570-27589 MsG0080048302.01.T01:CDS 35.0%
TTGCTCCTTTGTGGTTTATA+GGG + contig26end:27519-27538 MsG0080048302.01.T01:CDS 35.0%
TTTGCTCCTTTGTGGTTTAT+AGG + contig26end:27518-27537 MsG0080048302.01.T01:CDS 35.0%
! ATAAGGTTTACGATTTGGAC+AGG + contig26end:27677-27696 MsG0080048302.01.T01:CDS 35.0%
! TAAGGTTTACGATTTGGACA+GGG + contig26end:27678-27697 MsG0080048302.01.T01:CDS 35.0%
! TGGCATTAGCACTTTTAGTA+AGG + contig26end:27354-27373 MsG0080048302.01.T01:CDS 35.0%
!! GTGTGTCTTGTGTTGATTAT+TGG + contig26end:27428-27447 MsG0080048302.01.T01:CDS 35.0%
!!! TCTTTGGCTTTTTGTTCCAA+TGG + contig26end:27550-27569 MsG0080048302.01.T01:CDS 35.0%
ATGGATAAAGAAGGTAGCCA+AGG - contig26end:27611-27630 None:intergenic 40.0%
CAAGTGGGGTAACTACAAAA+GGG + contig26end:27387-27406 MsG0080048302.01.T01:CDS 40.0%
TCAAGTGGGGTAACTACAAA+AGG + contig26end:27386-27405 MsG0080048302.01.T01:CDS 40.0%
TCATCAACAAGAGCAAACCA+AGG - contig26end:27206-27225 None:intergenic 40.0%
TGAGAAGCAATGGAACAAGA+TGG + contig26end:27802-27821 MsG0080048302.01.T01:CDS 40.0%
! GCTCTTGTTGATGAAAGAAC+TGG + contig26end:27212-27231 MsG0080048302.01.T01:CDS 40.0%
CAATGGAACAAGATGGAGCA+AGG + contig26end:27809-27828 MsG0080048302.01.T01:CDS 45.0%
CCAGTCCCTATAAACCACAA+AGG - contig26end:27527-27546 None:intergenic 45.0%
CCTTTGTGGTTTATAGGGAC+TGG + contig26end:27524-27543 MsG0080048302.01.T01:CDS 45.0%
GATTTGGACAGGGTTTCTCT+TGG + contig26end:27688-27707 MsG0080048302.01.T01:CDS 45.0%
TCAACAAGAGCAAACCAAGG+TGG - contig26end:27203-27222 None:intergenic 45.0%
TTCCATTGCTTCTCAACCTC+CGG - contig26end:27797-27816 None:intergenic 45.0%
! TTATAGGGACTGGTGCTCTT+TGG + contig26end:27534-27553 MsG0080048302.01.T01:CDS 45.0%
!! ACATAGCAGTGGTTCTGATC+CGG + contig26end:27775-27794 MsG0080048302.01.T01:CDS 45.0%
ATCCGGAGGTTGAGAAGCAA+TGG + contig26end:27792-27811 MsG0080048302.01.T01:CDS 50.0%
GAAGGTAGCCAAGGAACCAA+AGG - contig26end:27602-27621 None:intergenic 50.0%
! GGGGAGTTCCTTTGGTTCCT+TGG + contig26end:27591-27610 MsG0080048302.01.T01:CDS 55.0%
!! TAGCAGTGGTTCTGATCCGG+AGG + contig26end:27778-27797 MsG0080048302.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
contig26end gene 27194 27874 27194 ID=MsG0080048302.01;Name=MsG0080048302.01
contig26end mRNA 27194 27874 27194 ID=MsG0080048302.01.T01;Parent=MsG0080048302.01;Name=MsG0080048302.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|226
contig26end exon 27194 27874 27194 ID=MsG0080048302.01.T01:exon:22972;Parent=MsG0080048302.01.T01
contig26end CDS 27194 27874 27194 ID=MsG0080048302.01.T01:cds;Parent=MsG0080048302.01.T01
Gene Sequence

>MsG0080048302.01.T01

ATGATGCCACCTTGGTTTGCTCTTGTTGATGAAAGAACTGGAACACCTATGAATGCTACAATCTCAATGCTTATTGCTACTGCTATTGTTGCTTTCTTCACCGATCTTAGTATTCTAGCTAACCTTTTGTCGATTTCTACTTTATTTATCTTTTCACTTGTGGCATTAGCACTTTTAGTAAGGAGATATTATTCAAGTGGGGTAACTACAAAAGGGAATCAAGTGAGTCTCATTGTGTGTCTTGTGTTGATTATTGGTTCTTCAATTGGAATTTCAGCTTATTGGGCTAATAGTAGTGAGCATAATTGGATTGGATATACAATCTTTGCTCCTTTGTGGTTTATAGGGACTGGTGCTCTTTGGCTTTTTGTTCCAATGGCAAAGAAGCCTAAATTATGGGGAGTTCCTTTGGTTCCTTGGCTACCTTCTTTATCCATTGCTATTAACATATTCCTTCTTGGAACTATTGATAAAAAATCGTATATAAGGTTTACGATTTGGACAGGGTTTCTCTTGGTATACTATGTGCTATTAGGGTTACATGCTTCATATGACACGGCAAAAGAATTTGATAAGAGTAAACATAGCAGTGGTTCTGATCCGGAGGTTGAGAAGCAATGGAACAAGATGGAGCAAGGTGCTGTAGAGCAAGATTCTAGTGTTGCACATGTTTCCAATTAA

Protein sequence

>MsG0080048302.01.T01

MMPPWFALVDERTGTPMNATISMLIATAIVAFFTDLSILANLLSISTLFIFSLVALALLVRRYYSSGVTTKGNQVSLIVCLVLIIGSSIGISAYWANSSEHNWIGYTIFAPLWFIGTGALWLFVPMAKKPKLWGVPLVPWLPSLSIAINIFLLGTIDKKSYIRFTIWTGFLLVYYVLLGLHASYDTAKEFDKSKHSSGSDPEVEKQWNKMEQGAVEQDSSVAHVSN*