AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003712.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003712.01.T01 MTR_1g068825 96.109 257 10 0 1 257 1 257 3.57e-176 485
MsG0180003712.01.T01 MTR_7g099950 44.314 255 125 5 1 243 85 334 1.07e-35 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003712.01.T01 AT3G53670 41.727 139 75 4 78 215 66 199 2.72e-16 75.5
MsG0180003712.01.T01 AT3G53670 41.727 139 75 4 78 215 66 199 2.72e-16 75.5
MsG0180003712.01.T01 AT2G37480 42.520 127 54 4 73 193 58 171 2.01e-14 70.1
MsG0180003712.01.T01 AT2G37480 41.732 127 54 4 73 193 58 170 1.83e-13 67.4

Find 81 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTACAAACGTGTCGTTTA+AGG 0.134390 1:+67220235 MsG0180003712.01.T01:five_prime_UTR
AAGCGATTTCAGATCGAAAT+TGG 0.164469 1:-67219887 None:intergenic
AAATGAAATTGTCTGTTAAA+TGG 0.168633 1:+67221955 MsG0180003712.01.T01:CDS
AATCCTTTGCCACAAGATTC+TGG 0.274308 1:-67222152 None:intergenic
GGTAGTAGTAACTGTAATAT+TGG 0.282358 1:+67221128 MsG0180003712.01.T01:CDS
AGTAAACTTGAAATTACTTT+TGG 0.301816 1:+67221044 MsG0180003712.01.T01:CDS
CTTGAAGGCAACACCGTATC+AGG 0.316264 1:-67221236 None:intergenic
GTAGTAGTAACTGTAATATT+GGG 0.335267 1:+67221129 MsG0180003712.01.T01:CDS
TTTCTCGCGGCTACCCTTCA+TGG 0.337552 1:-67221919 None:intergenic
CACTCTCCTGCGAATGTTGT+GGG 0.342078 1:+67221008 MsG0180003712.01.T01:CDS
TCCGACATGGCAAGGACTTT+CGG 0.354930 1:-67222258 None:intergenic
TTAAATAATCTCGACGTTGC+TGG 0.364240 1:+67222191 MsG0180003712.01.T01:CDS
TGCAATTTGATTCCGTTTCA+CGG 0.366326 1:+67219965 MsG0180003712.01.T01:five_prime_UTR
AGATCGAAGAACATGCGAAA+TGG 0.409219 1:-67219834 None:intergenic
AATGAAATTGTCTGTTAAAT+GGG 0.413068 1:+67221956 MsG0180003712.01.T01:CDS
CAAAGGATTTGATGCTTCAA+TGG 0.415687 1:+67222166 MsG0180003712.01.T01:CDS
CGAAATGGATCTGAGAGTTA+TGG 0.419952 1:-67219819 None:intergenic
TTGATTCACGGTTGAATTTG+AGG 0.426338 1:+67220133 MsG0180003712.01.T01:five_prime_UTR
AACCTTTCCTTATCCTGATA+CGG 0.431985 1:+67221223 MsG0180003712.01.T01:CDS
CTCTACACAATTTCCAGACT+CGG 0.432901 1:+67221304 MsG0180003712.01.T01:CDS
AATTGCGAAAATCTCAAAAC+AGG 0.441311 1:-67220209 None:intergenic
TCCAGACTCGGTTTCTCAGC+CGG 0.444606 1:+67221316 MsG0180003712.01.T01:CDS
CCACTCTCCTGCGAATGTTG+TGG 0.452810 1:+67221007 MsG0180003712.01.T01:CDS
TCGTCATCAGAAGAGCTTGA+AGG 0.452918 1:-67221251 None:intergenic
AGCGATTTCAGATCGAAATT+GGG 0.457477 1:-67219886 None:intergenic
CATATGTGGTGGGTTTGAAC+AGG 0.469286 1:+67221160 MsG0180003712.01.T01:CDS
TGGGAAATCCCTTGTTCGAT+CGG 0.471583 1:-67219867 None:intergenic
ACCGGCTGAGAAACCGAGTC+TGG 0.472530 1:-67221317 None:intergenic
CGATCGGTTCCGATCGAACA+AGG 0.475465 1:+67219858 MsG0180003712.01.T01:five_prime_UTR
GACTTATGCAGTCTTGATAT+AGG 0.475805 1:-67222312 None:intergenic
TGGCAAGGACTTTCGGTAGC+TGG 0.476961 1:-67222251 None:intergenic
AGAAGCAGTATCGCTATAGA+TGG 0.477970 1:+67222129 MsG0180003712.01.T01:CDS
GTGTGATCTTATACGATTGA+TGG 0.483125 1:+67220980 MsG0180003712.01.T01:intron
TCTGATGACGAGGAGGCTGA+TGG 0.485008 1:+67221263 MsG0180003712.01.T01:CDS
GGCAACACCGTATCAGGATA+AGG 0.496540 1:-67221230 None:intergenic
ACTAAAAGTGAAGAGCTTGA+AGG 0.497843 1:+67221193 MsG0180003712.01.T01:CDS
CATGTTCTTCGATCTTCGAT+CGG 0.503699 1:+67219842 MsG0180003712.01.T01:five_prime_UTR
CAGAAGGGCAGCTATTCTAA+AGG 0.505063 1:+67222089 MsG0180003712.01.T01:CDS
GGAGACAAAATCAAGGAAGT+TGG 0.512811 1:-67222291 None:intergenic
ATGTTGTTTCTTAGTTCTCA+CGG 0.517557 1:-67222021 None:intergenic
AAAGAAGATCCACCGTGAAA+CGG 0.518001 1:-67219977 None:intergenic
GGTACCGGATCATACACATC+AGG 0.520563 1:-67221981 None:intergenic
TTGACTTCAAAGATTGTGAA+AGG 0.525582 1:+67220170 MsG0180003712.01.T01:five_prime_UTR
AGAAGGGCAGCTATTCTAAA+GGG 0.525902 1:+67222090 MsG0180003712.01.T01:CDS
AAAACTTGTATCTGCCATGA+AGG 0.537609 1:+67221905 MsG0180003712.01.T01:CDS
GGGTAGCCGCGAGAAACACT+TGG 0.556191 1:+67221926 MsG0180003712.01.T01:CDS
TGGCCAGAATCTTGTGGCAA+AGG 0.557338 1:+67222149 MsG0180003712.01.T01:CDS
AGGTGTAAAGAAAAGTCAGA+AGG 0.565051 1:+67222073 MsG0180003712.01.T01:CDS
GAATTCGAATCGAATCGAAT+CGG 0.573482 1:-67219788 None:intergenic
TCGCACCCCACAACATTCGC+AGG 0.577188 1:-67221014 None:intergenic
TGTGACGACAATGTTGGTAC+CGG 0.579529 1:-67221996 None:intergenic
CTAAGAAACAACATAAATCC+CGG 0.580812 1:+67222030 MsG0180003712.01.T01:CDS
TGGTCATAGCTTCTCCGACA+TGG 0.589070 1:-67222271 None:intergenic
CCACAACATTCGCAGGAGAG+TGG 0.589259 1:-67221007 None:intergenic
ATTGGGGAGGGACACATATG+TGG 0.592835 1:+67221146 MsG0180003712.01.T01:CDS
ATAGCTTCTCCGACATGGCA+AGG 0.593702 1:-67222266 None:intergenic
AGTAACTGTAATATTGGGGA+GGG 0.594128 1:+67221134 MsG0180003712.01.T01:CDS
ATCAAGAAGAGTGAAAAGAA+AGG 0.600790 1:+67222053 MsG0180003712.01.T01:CDS
GGCCCCTGATGTGTATGATC+CGG 0.600919 1:+67221977 MsG0180003712.01.T01:CDS
GATCGGTTCCGATCGAACAA+GGG 0.602614 1:+67219859 MsG0180003712.01.T01:five_prime_UTR
TAGTAGTAACTGTAATATTG+GGG 0.605374 1:+67221130 MsG0180003712.01.T01:CDS
TGATATAGGAGACAAAATCA+AGG 0.608502 1:-67222298 None:intergenic
TTCAAGCTCTTCTGATGACG+AGG 0.614405 1:+67221253 MsG0180003712.01.T01:CDS
GGTGTAAAGAAAAGTCAGAA+GGG 0.615586 1:+67222074 MsG0180003712.01.T01:CDS
GGGGAGGGACACATATGTGG+TGG 0.617375 1:+67221149 MsG0180003712.01.T01:CDS
GGGAGGGACACATATGTGGT+GGG 0.620596 1:+67221150 MsG0180003712.01.T01:CDS
AAACTTGTATCTGCCATGAA+GGG 0.622758 1:+67221906 MsG0180003712.01.T01:CDS
ACCGAAAGTCCTTGCCATGT+CGG 0.626598 1:+67222257 MsG0180003712.01.T01:CDS
ACGGTATGTGACGACAATGT+TGG 0.627213 1:-67222002 None:intergenic
CACCGTATCAGGATAAGGAA+AGG 0.628831 1:-67221225 None:intergenic
TATAGATGGCCAGAATCTTG+TGG 0.629442 1:+67222143 MsG0180003712.01.T01:CDS
GGTAGCCGCGAGAAACACTT+GGG 0.637014 1:+67221927 MsG0180003712.01.T01:CDS
TAGTAACTGTAATATTGGGG+AGG 0.638150 1:+67221133 MsG0180003712.01.T01:CDS
ACTCTCCTGCGAATGTTGTG+GGG 0.649280 1:+67221009 MsG0180003712.01.T01:CDS
ACATGTATCTGATGATGATG+AGG 0.654147 1:+67221103 MsG0180003712.01.T01:CDS
AAGCTCTTCTGATGACGAGG+AGG 0.662047 1:+67221256 MsG0180003712.01.T01:CDS
GCGATCCCAAGTGTTTCTCG+CGG 0.669443 1:-67221932 None:intergenic
GTACCGGATCATACACATCA+GGG 0.681011 1:-67221980 None:intergenic
TACCGGATCATACACATCAG+GGG 0.711683 1:-67221979 None:intergenic
GTATCTGATGATGATGAGGA+TGG 0.713196 1:+67221107 MsG0180003712.01.T01:CDS
AATTTGATTCCGTTTCACGG+TGG 0.736766 1:+67219968 MsG0180003712.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAGATGAAAAAACTT+TGG - Chr1:67220085-67220104 None:intergenic 15.0%
!! AATACAAAATTGAAAATTCA+TGG - Chr1:67220342-67220361 None:intergenic 15.0%
!!! AAGTTTTTTCATCTATTTTT+TGG + Chr1:67220085-67220104 MsG0180003712.01.T01:five_prime_UTR 15.0%
!! AAATGAAATTGTCTGTTAAA+TGG + Chr1:67221955-67221974 MsG0180003712.01.T01:CDS 20.0%
!! AATGAAATTGTCTGTTAAAT+GGG + Chr1:67221956-67221975 MsG0180003712.01.T01:CDS 20.0%
!! GCTAATTAATGAATATAACA+AGG + Chr1:67220518-67220537 MsG0180003712.01.T01:intron 20.0%
!! TAAGAAATGTAGTTTATGTT+TGG + Chr1:67221391-67221410 MsG0180003712.01.T01:intron 20.0%
!! TAATTAGCCATATAAATTGT+CGG - Chr1:67220506-67220525 None:intergenic 20.0%
!! TCAACAAATTAGATAGTTAA+CGG - Chr1:67220679-67220698 None:intergenic 20.0%
!! TTTACAATATTAGTGTATAG+TGG + Chr1:67220757-67220776 MsG0180003712.01.T01:intron 20.0%
!!! AGTAAACTTGAAATTACTTT+TGG + Chr1:67221044-67221063 MsG0180003712.01.T01:CDS 20.0%
! AAATGTAGTTTATGTTTGGA+CGG + Chr1:67221395-67221414 MsG0180003712.01.T01:intron 25.0%
! AACATCTTGAAAAAAGTGAT+AGG + Chr1:67220011-67220030 MsG0180003712.01.T01:five_prime_UTR 25.0%
! AAGTGTATGTTTGTATCATA+TGG - Chr1:67221575-67221594 None:intergenic 25.0%
! ATATTAGTGTATAGTGGTAT+TGG + Chr1:67220763-67220782 MsG0180003712.01.T01:intron 25.0%
! ATATTTGCCGACAATTTATA+TGG + Chr1:67220496-67220515 MsG0180003712.01.T01:intron 25.0%
! CTATATTCAACCTAGAAATT+AGG - Chr1:67220318-67220337 None:intergenic 25.0%
! GTAGTAGTAACTGTAATATT+GGG + Chr1:67221129-67221148 MsG0180003712.01.T01:CDS 25.0%
! TAGTAGTAACTGTAATATTG+GGG + Chr1:67221130-67221149 MsG0180003712.01.T01:CDS 25.0%
! TATGATTCAACCTAATTTCT+AGG + Chr1:67220305-67220324 MsG0180003712.01.T01:intron 25.0%
!! CTAATTTCTAGGTTGAATAT+AGG + Chr1:67220316-67220335 MsG0180003712.01.T01:intron 25.0%
!!! AAACTTGAAATTACTTTTGG+TGG + Chr1:67221047-67221066 MsG0180003712.01.T01:CDS 25.0%
!!! AAATCGCTTTTGTAACAATT+AGG + Chr1:67219901-67219920 MsG0180003712.01.T01:five_prime_UTR 25.0%
!!! AACTTGAAATTACTTTTGGT+GGG + Chr1:67221048-67221067 MsG0180003712.01.T01:CDS 25.0%
!!! AATCGCTTTTGTAACAATTA+GGG + Chr1:67219902-67219921 MsG0180003712.01.T01:five_prime_UTR 25.0%
!!! ACATACACTTTATTTTGAAG+TGG + Chr1:67221585-67221604 MsG0180003712.01.T01:intron 25.0%
!!! ATTTTTTGGAGTATTTCTTC+TGG + Chr1:67220099-67220118 MsG0180003712.01.T01:five_prime_UTR 25.0%
!!! GTTTTGTGAATTTTGATTCA+CGG + Chr1:67220121-67220140 MsG0180003712.01.T01:five_prime_UTR 25.0%
!!! TGTGTTTTTTCTGTTAATTG+AGG - Chr1:67219754-67219773 None:intergenic 25.0%
AATTGCGAAAATCTCAAAAC+AGG - Chr1:67220212-67220231 None:intergenic 30.0%
ATCAAGAAGAGTGAAAAGAA+AGG + Chr1:67222053-67222072 MsG0180003712.01.T01:CDS 30.0%
CATGATTTATCAGCTATGAA+TGG + Chr1:67220444-67220463 MsG0180003712.01.T01:intron 30.0%
CTAAGAAACAACATAAATCC+CGG + Chr1:67222030-67222049 MsG0180003712.01.T01:CDS 30.0%
GGTAGTAGTAACTGTAATAT+TGG + Chr1:67221128-67221147 MsG0180003712.01.T01:CDS 30.0%
GGTTAATTGTGTTCAATTGT+TGG - Chr1:67220560-67220579 None:intergenic 30.0%
GTAATCAACAAGTAAGAAGA+AGG - Chr1:67220831-67220850 None:intergenic 30.0%
GTATGTTTGTATCATATGGT+GGG - Chr1:67221571-67221590 None:intergenic 30.0%
TGAACACAATTAGAACTTAC+CGG - Chr1:67221338-67221357 None:intergenic 30.0%
TGATATAGGAGACAAAATCA+AGG - Chr1:67222301-67222320 None:intergenic 30.0%
TGTATGTTTGTATCATATGG+TGG - Chr1:67221572-67221591 None:intergenic 30.0%
TTGACTTCAAAGATTGTGAA+AGG + Chr1:67220170-67220189 MsG0180003712.01.T01:five_prime_UTR 30.0%
TTGTAGCTTTCATACAATCA+CGG + Chr1:67221522-67221541 MsG0180003712.01.T01:intron 30.0%
! AACGAAACTAAAAAAGCGTA+AGG - Chr1:67220055-67220074 None:intergenic 30.0%
! TTATGCTAGATGCATTGTTT+TGG + Chr1:67221713-67221732 MsG0180003712.01.T01:intron 30.0%
! TTTTCTCTGTATTATACCAC+AGG + Chr1:67221875-67221894 MsG0180003712.01.T01:intron 30.0%
!! ATGTTGTTTCTTAGTTCTCA+CGG - Chr1:67222024-67222043 None:intergenic 30.0%
!!! ATAGTTTTGTCGAGCATAAT+TGG + Chr1:67221816-67221835 MsG0180003712.01.T01:intron 30.0%
!!! GTTTTGTCGATTTGTTCTTT+GGG + Chr1:67220952-67220971 MsG0180003712.01.T01:intron 30.0%
!!! TGTTTTGTCGATTTGTTCTT+TGG + Chr1:67220951-67220970 MsG0180003712.01.T01:intron 30.0%
AAAACTTGTATCTGCCATGA+AGG + Chr1:67221905-67221924 MsG0180003712.01.T01:CDS 35.0%
AAACTTGTATCTGCCATGAA+GGG + Chr1:67221906-67221925 MsG0180003712.01.T01:CDS 35.0%
AACCTTTCCTTATCCTGATA+CGG + Chr1:67221223-67221242 MsG0180003712.01.T01:CDS 35.0%
AAGCGATTTCAGATCGAAAT+TGG - Chr1:67219890-67219909 None:intergenic 35.0%
AAGTATAACAGTCGACACAT+AGG - Chr1:67220877-67220896 None:intergenic 35.0%
ACGGTTTGACACATTTAAGT+TGG - Chr1:67220660-67220679 None:intergenic 35.0%
ACTAAAAGTGAAGAGCTTGA+AGG + Chr1:67221193-67221212 MsG0180003712.01.T01:CDS 35.0%
AGCGATTTCAGATCGAAATT+GGG - Chr1:67219889-67219908 None:intergenic 35.0%
AGGTGTAAAGAAAAGTCAGA+AGG + Chr1:67222073-67222092 MsG0180003712.01.T01:CDS 35.0%
AGTAACTGTAATATTGGGGA+GGG + Chr1:67221134-67221153 MsG0180003712.01.T01:CDS 35.0%
ATGGTAAGTGATGTTCCAAA+GGG - Chr1:67220584-67220603 None:intergenic 35.0%
CAAAGGATTTGATGCTTCAA+TGG + Chr1:67222166-67222185 MsG0180003712.01.T01:CDS 35.0%
GAAATTACTGTGAACAACTG+TGG + Chr1:67221474-67221493 MsG0180003712.01.T01:intron 35.0%
GAATAGTAGACAATGTTGTC+TGG + Chr1:67220729-67220748 MsG0180003712.01.T01:intron 35.0%
GAATTCGAATCGAATCGAAT+CGG - Chr1:67219791-67219810 None:intergenic 35.0%
GGTGTAAAGAAAAGTCAGAA+GGG + Chr1:67222074-67222093 MsG0180003712.01.T01:CDS 35.0%
GTGTGATCTTATACGATTGA+TGG + Chr1:67220980-67220999 MsG0180003712.01.T01:intron 35.0%
TAGTAACTGTAATATTGGGG+AGG + Chr1:67221133-67221152 MsG0180003712.01.T01:CDS 35.0%
TGCAATTTGATTCCGTTTCA+CGG + Chr1:67219965-67219984 MsG0180003712.01.T01:five_prime_UTR 35.0%
TTAAATAATCTCGACGTTGC+TGG + Chr1:67222191-67222210 MsG0180003712.01.T01:CDS 35.0%
TTGATTCACGGTTGAATTTG+AGG + Chr1:67220133-67220152 MsG0180003712.01.T01:five_prime_UTR 35.0%
TTGTGAACACTTGCAAATCA+TGG - Chr1:67220603-67220622 None:intergenic 35.0%
! ACATGTATCTGATGATGATG+AGG + Chr1:67221103-67221122 MsG0180003712.01.T01:CDS 35.0%
! CTATAGCTTTTGCAAAACCA+CGG - Chr1:67221447-67221466 None:intergenic 35.0%
! GTTCTGAACAATAAGACACT+AGG - Chr1:67220375-67220394 None:intergenic 35.0%
! TCTTTTCACTCTTCTTGATC+CGG - Chr1:67222052-67222071 None:intergenic 35.0%
!! ATCTACAAACGTGTCGTTTA+AGG + Chr1:67220235-67220254 MsG0180003712.01.T01:five_prime_UTR 35.0%
!!! TTGTTTTGGTCGAGCATAAT+TGG + Chr1:67221727-67221746 MsG0180003712.01.T01:intron 35.0%
!!! TTTTTGAGATGCTAGTTGCA+TGG - Chr1:67222229-67222248 None:intergenic 35.0%
AAAGAAGATCCACCGTGAAA+CGG - Chr1:67219980-67219999 None:intergenic 40.0%
AATCCTTTGCCACAAGATTC+TGG - Chr1:67222155-67222174 None:intergenic 40.0%
AATTTGATTCCGTTTCACGG+TGG + Chr1:67219968-67219987 MsG0180003712.01.T01:five_prime_UTR 40.0%
ACGGTTGCCCATAATGATTT+CGG - Chr1:67221428-67221447 None:intergenic 40.0%
AGAAGCAGTATCGCTATAGA+TGG + Chr1:67222129-67222148 MsG0180003712.01.T01:CDS 40.0%
AGAAGGGCAGCTATTCTAAA+GGG + Chr1:67222090-67222109 MsG0180003712.01.T01:CDS 40.0%
AGATCGAAGAACATGCGAAA+TGG - Chr1:67219837-67219856 None:intergenic 40.0%
CATGGTAAGTGATGTTCCAA+AGG - Chr1:67220585-67220604 None:intergenic 40.0%
CATGTTCTTCGATCTTCGAT+CGG + Chr1:67219842-67219861 MsG0180003712.01.T01:five_prime_UTR 40.0%
CGAAATGGATCTGAGAGTTA+TGG - Chr1:67219822-67219841 None:intergenic 40.0%
CTCTACACAATTTCCAGACT+CGG + Chr1:67221304-67221323 MsG0180003712.01.T01:CDS 40.0%
CTTGAAAAAAGTGATAGGCG+TGG + Chr1:67220016-67220035 MsG0180003712.01.T01:five_prime_UTR 40.0%
GAACACAATTAACCACCCTT+TGG + Chr1:67220566-67220585 MsG0180003712.01.T01:intron 40.0%
GGAGACAAAATCAAGGAAGT+TGG - Chr1:67222294-67222313 None:intergenic 40.0%
GTAGTTTATGTTTGGACGGT+GGG + Chr1:67221399-67221418 MsG0180003712.01.T01:intron 40.0%
TATAGATGGCCAGAATCTTG+TGG + Chr1:67222143-67222162 MsG0180003712.01.T01:CDS 40.0%
TGGGTTGCCGAAATCATTAT+GGG + Chr1:67221418-67221437 MsG0180003712.01.T01:intron 40.0%
TGTAGTTTATGTTTGGACGG+TGG + Chr1:67221398-67221417 MsG0180003712.01.T01:intron 40.0%
! ATGGCAGATACAAGTTTTGC+AGG - Chr1:67221903-67221922 None:intergenic 40.0%
! CTTTTCACTCTTCTTGATCC+GGG - Chr1:67222051-67222070 None:intergenic 40.0%
! GTATCTGATGATGATGAGGA+TGG + Chr1:67221107-67221126 MsG0180003712.01.T01:CDS 40.0%
!! CAAGCTCTTCACTTTTAGTG+TGG - Chr1:67221192-67221211 None:intergenic 40.0%
ACGGTATGTGACGACAATGT+TGG - Chr1:67222005-67222024 None:intergenic 45.0%
ACGGTTGCTAGCCATGATTT+CGG + Chr1:67221541-67221560 MsG0180003712.01.T01:intron 45.0%
CACCGTATCAGGATAAGGAA+AGG - Chr1:67221228-67221247 None:intergenic 45.0%
CAGAAGGGCAGCTATTCTAA+AGG + Chr1:67222089-67222108 MsG0180003712.01.T01:CDS 45.0%
GAAATCATTATGGGCAACCG+TGG + Chr1:67221427-67221446 MsG0180003712.01.T01:intron 45.0%
GTAAGTGATGTTCCAAAGGG+TGG - Chr1:67220581-67220600 None:intergenic 45.0%
GTACCGGATCATACACATCA+GGG - Chr1:67221983-67222002 None:intergenic 45.0%
TACCGGATCATACACATCAG+GGG - Chr1:67221982-67222001 None:intergenic 45.0%
TCATTCGCCAGATAGAAAGC+TGG + Chr1:67221622-67221641 MsG0180003712.01.T01:intron 45.0%
TCGTCATCAGAAGAGCTTGA+AGG - Chr1:67221254-67221273 None:intergenic 45.0%
TGGGAAATCCCTTGTTCGAT+CGG - Chr1:67219870-67219889 None:intergenic 45.0%
TGTGACGACAATGTTGGTAC+CGG - Chr1:67221999-67222018 None:intergenic 45.0%
! GTGGGTTGCCGAAATCATTA+TGG + Chr1:67221417-67221436 MsG0180003712.01.T01:intron 45.0%
! TTCAAGCTCTTCTGATGACG+AGG + Chr1:67221253-67221272 MsG0180003712.01.T01:CDS 45.0%
!! CATATGTGGTGGGTTTGAAC+AGG + Chr1:67221160-67221179 MsG0180003712.01.T01:CDS 45.0%
ACCGAAAGTCCTTGCCATGT+CGG + Chr1:67222257-67222276 MsG0180003712.01.T01:CDS 50.0%
ACTCTCCTGCGAATGTTGTG+GGG + Chr1:67221009-67221028 MsG0180003712.01.T01:CDS 50.0%
ATAGAAAGCTGGTCGCTCCA+AGG + Chr1:67221633-67221652 MsG0180003712.01.T01:intron 50.0%
ATAGCTTCTCCGACATGGCA+AGG - Chr1:67222269-67222288 None:intergenic 50.0%
ATTGGGGAGGGACACATATG+TGG + Chr1:67221146-67221165 MsG0180003712.01.T01:CDS 50.0%
CACTCTCCTGCGAATGTTGT+GGG + Chr1:67221008-67221027 MsG0180003712.01.T01:CDS 50.0%
CTTGAAGGCAACACCGTATC+AGG - Chr1:67221239-67221258 None:intergenic 50.0%
GGCAACACCGTATCAGGATA+AGG - Chr1:67221233-67221252 None:intergenic 50.0%
GGTACGCGAGCTCTATGATT+AGG - Chr1:67220856-67220875 None:intergenic 50.0%
TAGAAAGCTGGTCGCTCCAA+GGG + Chr1:67221634-67221653 MsG0180003712.01.T01:intron 50.0%
TCCGACATGGCAAGGACTTT+CGG - Chr1:67222261-67222280 None:intergenic 50.0%
TGGCCAGAATCTTGTGGCAA+AGG + Chr1:67222149-67222168 MsG0180003712.01.T01:CDS 50.0%
TGGTCATAGCTTCTCCGACA+TGG - Chr1:67222274-67222293 None:intergenic 50.0%
! AAGCTCTTCTGATGACGAGG+AGG + Chr1:67221256-67221275 MsG0180003712.01.T01:CDS 50.0%
! ACAAGTTTTGCAGGTGCCTG+TGG - Chr1:67221894-67221913 None:intergenic 50.0%
! GATCGGTTCCGATCGAACAA+GGG + Chr1:67219859-67219878 MsG0180003712.01.T01:five_prime_UTR 50.0%
! GGTACCGGATCATACACATC+AGG - Chr1:67221984-67222003 None:intergenic 50.0%
!! TGGTGGGTTAGCCGAAATCA+TGG - Chr1:67221555-67221574 None:intergenic 50.0%
AGAAAGCTGGTCGCTCCAAG+GGG + Chr1:67221635-67221654 MsG0180003712.01.T01:intron 55.0%
CAACTACTTTCCACCCCCCT+TGG - Chr1:67221653-67221672 None:intergenic 55.0%
CAAGGGGGGTGGAAAGTAGT+TGG + Chr1:67221651-67221670 MsG0180003712.01.T01:intron 55.0%
CCACAACATTCGCAGGAGAG+TGG - Chr1:67221010-67221029 None:intergenic 55.0%
CCACTCTCCTGCGAATGTTG+TGG + Chr1:67221007-67221026 MsG0180003712.01.T01:CDS 55.0%
GCGATCCCAAGTGTTTCTCG+CGG - Chr1:67221935-67221954 None:intergenic 55.0%
GGAGCGACCAGCTTTCTATC+TGG - Chr1:67221632-67221651 None:intergenic 55.0%
GGCCCCTGATGTGTATGATC+CGG + Chr1:67221977-67221996 MsG0180003712.01.T01:CDS 55.0%
GGGAGGGACACATATGTGGT+GGG + Chr1:67221150-67221169 MsG0180003712.01.T01:CDS 55.0%
GGTAGCCGCGAGAAACACTT+GGG + Chr1:67221927-67221946 MsG0180003712.01.T01:CDS 55.0%
TGGCAAGGACTTTCGGTAGC+TGG - Chr1:67222254-67222273 None:intergenic 55.0%
TTTCTCGCGGCTACCCTTCA+TGG - Chr1:67221922-67221941 None:intergenic 55.0%
! CGATCGGTTCCGATCGAACA+AGG + Chr1:67219858-67219877 MsG0180003712.01.T01:five_prime_UTR 55.0%
! TCCAGACTCGGTTTCTCAGC+CGG + Chr1:67221316-67221335 MsG0180003712.01.T01:CDS 55.0%
! TCTGATGACGAGGAGGCTGA+TGG + Chr1:67221263-67221282 MsG0180003712.01.T01:CDS 55.0%
AAAGCTGGTCGCTCCAAGGG+GGG + Chr1:67221637-67221656 MsG0180003712.01.T01:intron 60.0%
ACCGGCTGAGAAACCGAGTC+TGG - Chr1:67221320-67221339 None:intergenic 60.0%
GAAAGCTGGTCGCTCCAAGG+GGG + Chr1:67221636-67221655 MsG0180003712.01.T01:intron 60.0%
GGGGAGGGACACATATGTGG+TGG + Chr1:67221149-67221168 MsG0180003712.01.T01:CDS 60.0%
GGGTAGCCGCGAGAAACACT+TGG + Chr1:67221926-67221945 MsG0180003712.01.T01:CDS 60.0%
TCGCACCCCACAACATTCGC+AGG - Chr1:67221017-67221036 None:intergenic 60.0%
GCTGGTCGCTCCAAGGGGGG+TGG + Chr1:67221640-67221659 MsG0180003712.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 67219748 67222331 67219748 ID=MsG0180003712.01;Name=MsG0180003712.01
Chr1 mRNA 67219748 67222331 67219748 ID=MsG0180003712.01.T01;Parent=MsG0180003712.01;Name=MsG0180003712.01.T01;_AED=0.30;_eAED=0.30;_QI=527|1|0.66|1|0.5|0.33|3|0|257
Chr1 exon 67219748 67220256 67219748 ID=MsG0180003712.01.T01:exon:51550;Parent=MsG0180003712.01.T01
Chr1 exon 67220981 67221337 67220981 ID=MsG0180003712.01.T01:exon:51551;Parent=MsG0180003712.01.T01
Chr1 exon 67221897 67222331 67221897 ID=MsG0180003712.01.T01:exon:51552;Parent=MsG0180003712.01.T01
Chr1 five_prime_UTR 67219748 67220256 67219748 ID=MsG0180003712.01.T01:five_prime_utr;Parent=MsG0180003712.01.T01
Chr1 five_prime_UTR 67220981 67220998 67220981 ID=MsG0180003712.01.T01:five_prime_utr;Parent=MsG0180003712.01.T01
Chr1 CDS 67220999 67221337 67220999 ID=MsG0180003712.01.T01:cds;Parent=MsG0180003712.01.T01
Chr1 CDS 67221897 67222331 67221897 ID=MsG0180003712.01.T01:cds;Parent=MsG0180003712.01.T01
Gene Sequence

>MsG0180003712.01.T01

ATGGACATCCACTCTCCTGCGAATGTTGTGGGGTGCGATATCATAAGTAAACTTGAAATTACTTTTGGTGGGTCATTACATATACAAGATGATCAAAAGTTAGAACATGTATCTGATGATGATGAGGATGGTAGTAGTAACTGTAATATTGGGGAGGGACACATATGTGGTGGGTTTGAACAGGAAGAAACCACACTAAAAGTGAAGAGCTTGAAGGAGTGTTCAACCTTTCCTTATCCTGATACGGTGTTGCCTTCAAGCTCTTCTGATGACGAGGAGGCTGATGGAGCATTGACAGAGTCACTCTCTACACAATTTCCAGACTCGGTTTCTCAGCCGGCACCTGCAAAACTTGTATCTGCCATGAAGGGTAGCCGCGAGAAACACTTGGGATCGCAAATGAAATTGTCTGTTAAATGGGCCCCTGATGTGTATGATCCGGTACCAACATTGTCGTCACATACCGTGAGAACTAAGAAACAACATAAATCCCGGATCAAGAAGAGTGAAAAGAAAGGTGTAAAGAAAAGTCAGAAGGGCAGCTATTCTAAAGGGTGTAGCAGCAAAGATAAGAAGCAGTATCGCTATAGATGGCCAGAATCTTGTGGCAAAGGATTTGATGCTTCAATGGAATTAAATAATCTCGACGTTGCTGGCTATGATTCATACCATGCAACTAGCATCTCAAAAATACCAGCTACCGAAAGTCCTTGCCATGTCGGAGAAGCTATGACCAACTTCCTTGATTTTGTCTCCTATATCAAGACTGCATAA
Protein sequence

>MsG0180003712.01.T01

MDIHSPANVVGCDIISKLEITFGGSLHIQDDQKLEHVSDDDEDGSSNCNIGEGHICGGFEQEETTLKVKSLKECSTFPYPDTVLPSSSSDDEEADGALTESLSTQFPDSVSQPAPAKLVSAMKGSREKHLGSQMKLSVKWAPDVYDPVPTLSSHTVRTKKQHKSRIKKSEKKGVKKSQKGSYSKGCSSKDKKQYRYRWPESCGKGFDASMELNNLDVAGYDSYHATSISKIPATESPCHVGEAMTNFLDFVSYIKTA*