AlfalfaGEDB | MsG0180004296.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004296.01

KEGG: K13448
GO: GO:0005509
PFAM: PF00036 PF13499

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004296.01.T01	XP_013468780.1	69.333	150	7	1	1	111	1	150	6.13E-59	188

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004296.01.T01	O23320	57.534	146	23	1	4	110	5	150	1.59E-48	154

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004296.01.T01	A0A072VLW5	69.333	150	7	1	1	111	1	150	2.93e-59	188

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003240.01	MsG0180004296.01	0.817164	3.800229e-52	2.746835e-49
MsG0180004181.01	MsG0180004296.01	0.902549	8.654283e-79	1.232181e-74
MsG0180004203.01	MsG0180004296.01	0.812177	4.833123e-51	3.051483e-48
MsG0180004296.01	MsG0380011613.01	0.821831	3.275882e-53	2.694898e-50
MsG0180004296.01	MsG0480018807.01	0.827329	1.661693e-54	1.599285e-51
MsG0180004296.01	MsG0480023349.01	0.810194	1.301004e-50	7.791768e-48
MsG0180004296.01	MsG0780039968.01	0.814879	1.230261e-51	8.353405e-49
MsG0180004296.01	MsG0880042810.01	0.803510	3.368939e-49	1.696151e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004296.01.T01	MTR_1g076650	69.333	150	7	1	1	111	1	150	7.42e-63	188
MsG0180004296.01.T01	MTR_1g041285	58.389	149	23	1	1	110	1	149	1.17e-51	160
MsG0180004296.01.T01	MTR_5g088320	52.027	148	32	1	1	109	1	148	6.56e-46	145
MsG0180004296.01.T01	MTR_6g025320	51.351	148	33	1	1	109	1	148	2.81e-45	144
MsG0180004296.01.T01	MTR_7g087610	51.351	148	33	1	1	109	1	148	7.04e-45	142
MsG0180004296.01.T01	MTR_0168s0120	64.130	92	30	1	21	109	21	112	1.58e-37	123
MsG0180004296.01.T01	MTR_6g025320	64.130	92	30	1	21	109	21	112	1.58e-37	123
MsG0180004296.01.T01	MTR_1g071150	45.890	146	41	2	1	108	1	146	3.72e-35	118
MsG0180004296.01.T01	MTR_7g115040	43.624	149	45	2	1	110	1	149	6.56e-32	110
MsG0180004296.01.T01	MTR_1g032070	51.648	91	41	1	21	108	91	181	6.60e-22	85.9
MsG0180004296.01.T01	MTR_1g032070	41.935	62	36	0	45	106	45	106	6.22e-12	59.7
MsG0180004296.01.T01	MTR_3g055510	42.857	105	46	1	1	105	41	131	9.51e-21	81.3
MsG0180004296.01.T01	MTR_8g066630	33.333	147	56	2	5	109	10	156	1.53e-20	81.3
MsG0180004296.01.T01	MTR_5g011850	30.822	146	64	2	1	109	1	146	4.22e-20	79.7
MsG0180004296.01.T01	MTR_8g107110	51.111	90	42	2	20	107	68	157	7.25e-20	79.7
MsG0180004296.01.T01	MTR_5g079340	47.778	90	45	2	20	107	48	137	7.91e-20	79.0
MsG0180004296.01.T01	MTR_5g079340	42.623	61	35	0	44	104	2	62	6.56e-11	55.8
MsG0180004296.01.T01	MTR_6g079570	49.425	87	42	1	23	107	74	160	6.73e-19	77.4
MsG0180004296.01.T01	MTR_4g112460	41.304	92	49	1	21	107	56	147	7.74e-19	77.0
MsG0180004296.01.T01	MTR_8g105230	48.837	86	41	1	21	103	81	166	1.74e-18	76.6
MsG0180004296.01.T01	MTR_8g105230	47.541	61	32	0	46	106	36	96	2.38e-12	60.5
MsG0180004296.01.T01	MTR_7g074020	32.877	146	59	2	3	109	21	166	3.22e-18	75.5
MsG0180004296.01.T01	MTR_3g055570	43.333	90	39	1	16	105	92	169	4.06e-18	75.5
MsG0180004296.01.T01	MTR_1g046950	40.179	112	62	2	1	107	29	140	6.90e-18	73.9
MsG0180004296.01.T01	MTR_3g067610	46.591	88	46	1	20	107	49	135	8.14e-18	73.6
MsG0180004296.01.T01	MTR_3g067610	46.667	60	32	0	44	103	3	62	6.66e-12	58.5
MsG0180004296.01.T01	MTR_3g055480	57.407	54	23	0	54	107	53	106	1.15e-17	72.8
MsG0180004296.01.T01	MTR_5g089440	38.393	112	61	3	1	107	68	176	4.58e-17	73.2
MsG0180004296.01.T01	MTR_3g055520	36.154	130	59	2	1	106	41	170	5.02e-17	72.4
MsG0180004296.01.T01	MTR_7g090450	45.918	98	46	2	17	107	115	212	5.92e-17	73.2
MsG0180004296.01.T01	MTR_7g075040	43.000	100	47	2	18	107	91	190	9.61e-17	72.4
MsG0180004296.01.T01	MTR_1g047100	45.161	93	47	1	21	109	49	141	1.26e-16	70.9
MsG0180004296.01.T01	MTR_4g067270	44.086	93	45	3	21	107	50	141	1.30e-16	70.9
MsG0180004296.01.T01	MTR_4g103630	38.739	111	62	2	1	107	31	139	1.33e-16	70.5
MsG0180004296.01.T01	MTR_3g089070	36.111	108	66	2	3	107	32	139	2.21e-16	70.1
MsG0180004296.01.T01	MTR_3g089090	36.111	108	66	2	3	107	31	138	2.49e-16	69.7
MsG0180004296.01.T01	MTR_6g007613	31.250	144	60	2	3	107	20	163	4.10e-16	70.1
MsG0180004296.01.T01	MTR_4g103655	37.838	111	63	2	1	107	31	139	6.02e-16	68.9
MsG0180004296.01.T01	MTR_5g017550	40.206	97	56	1	15	109	87	183	1.24e-15	69.3
MsG0180004296.01.T01	MTR_4g103650	36.937	111	64	2	1	107	27	135	1.96e-15	67.4
MsG0180004296.01.T01	MTR_3g055585	35.385	130	60	2	1	106	41	170	3.07e-15	67.8
MsG0180004296.01.T01	MTR_7g106710	43.810	105	52	3	5	107	472	571	4.74e-15	69.7
MsG0180004296.01.T01	MTR_8g069915	35.870	92	54	1	21	107	57	148	1.25e-14	66.2
MsG0180004296.01.T01	MTR_8g036075	53.731	67	30	1	42	107	125	191	1.44e-14	66.6
MsG0180004296.01.T01	MTR_4g086260	36.275	102	64	1	7	107	88	189	3.17e-14	65.5
MsG0180004296.01.T01	MTR_5g017510	39.175	97	57	1	15	109	88	184	3.65e-14	65.5
MsG0180004296.01.T01	MTR_5g017560	39.175	97	57	1	15	109	88	184	3.65e-14	65.5
MsG0180004296.01.T01	MTR_4g115170	53.030	66	29	1	44	107	151	216	9.38e-14	65.1
MsG0180004296.01.T01	MTR_6g023460	39.175	97	53	2	16	108	48	142	1.23e-13	63.2
MsG0180004296.01.T01	MTR_4g082050	38.542	96	48	1	12	107	54	138	1.25e-13	63.2
MsG0180004296.01.T01	MTR_8g078270	50.000	64	32	0	44	107	51	114	2.63e-13	63.2
MsG0180004296.01.T01	MTR_6g007613	47.619	63	33	0	45	107	28	90	2.74e-13	62.4
MsG0180004296.01.T01	MTR_6g007613	29.231	130	53	2	3	93	20	149	3.44e-11	57.0
MsG0180004296.01.T01	MTR_7g074020	47.619	63	33	0	45	107	29	91	2.99e-13	62.4
MsG0180004296.01.T01	MTR_7g074020	30.769	130	51	2	3	93	21	150	5.78e-13	61.6
MsG0180004296.01.T01	MTR_3g083470	40.476	84	47	1	28	108	135	218	5.32e-13	62.8
MsG0180004296.01.T01	MTR_1g055255	32.414	145	58	3	1	107	418	560	7.54e-13	63.5
MsG0180004296.01.T01	MTR_1g054865	31.034	145	60	3	1	107	455	597	7.87e-13	63.5
MsG0180004296.01.T01	MTR_7g074240	34.459	148	46	4	13	109	25	172	2.42e-12	60.5
MsG0180004296.01.T01	MTR_7g068710	29.932	147	65	1	1	109	350	496	3.14e-12	62.0
MsG0180004296.01.T01	MTR_8g095440	31.724	145	59	3	1	107	324	466	4.26e-12	61.6
MsG0180004296.01.T01	MTR_3g098070	31.724	145	59	3	1	107	323	465	4.55e-12	61.2
MsG0180004296.01.T01	MTR_1g096490	33.103	145	57	3	1	107	324	466	5.86e-12	60.8
MsG0180004296.01.T01	MTR_4g107490	39.785	93	50	4	21	109	423	513	1.38e-11	60.1
MsG0180004296.01.T01	MTR_0028s0170	37.349	83	41	1	26	108	374	445	1.47e-11	60.1
MsG0180004296.01.T01	MTR_5g099240	31.034	145	60	3	1	107	318	460	1.82e-11	59.7
MsG0180004296.01.T01	MTR_5g008695	39.130	92	49	3	21	106	22	112	1.89e-11	56.6
MsG0180004296.01.T01	MTR_5g099240	31.034	145	60	3	1	107	390	532	1.94e-11	59.7
MsG0180004296.01.T01	MTR_3g098090	30.345	145	61	3	1	107	326	468	2.37e-11	59.3
MsG0180004296.01.T01	MTR_7g089760	38.053	113	60	4	1	107	33	141	2.38e-11	57.4
MsG0180004296.01.T01	MTR_2g098890	41.053	95	47	3	21	107	109	202	2.46e-11	58.2
MsG0180004296.01.T01	MTR_5g089320	30.612	147	59	5	1	107	362	505	4.24e-11	58.5
MsG0180004296.01.T01	MTR_5g025690	47.692	65	32	1	45	107	131	195	4.65e-11	57.4
MsG0180004296.01.T01	MTR_1g041150	31.724	145	59	3	1	107	412	554	5.63e-11	58.2
MsG0180004296.01.T01	MTR_5g009830	30.612	147	59	5	1	107	360	503	7.02e-11	58.2
MsG0180004296.01.T01	MTR_5g009830	41.935	62	36	0	47	108	372	433	9.03e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004296.01.T01	AT4G14640	57.534	146	23	1	4	110	5	150	1.62e-49	154
MsG0180004296.01.T01	AT3G22930	57.343	143	22	1	5	108	28	170	9.91e-49	153
MsG0180004296.01.T01	AT5G37780	52.703	148	31	1	1	109	1	148	1.83e-46	147
MsG0180004296.01.T01	AT1G66410	52.703	148	31	1	1	109	1	148	1.83e-46	147
MsG0180004296.01.T01	AT2G27030	52.000	150	33	1	1	111	1	150	4.80e-46	147
MsG0180004296.01.T01	AT3G56800	52.027	148	32	1	1	109	1	148	1.10e-45	144
MsG0180004296.01.T01	AT2G27030	52.027	148	32	1	1	109	1	148	1.10e-45	144
MsG0180004296.01.T01	AT2G41110	52.027	148	32	1	1	109	1	148	1.10e-45	144
MsG0180004296.01.T01	AT5G21274	52.027	148	32	1	1	109	1	148	1.34e-45	144
MsG0180004296.01.T01	AT3G43810	51.351	148	33	1	1	109	1	148	2.94e-45	144
MsG0180004296.01.T01	AT3G43810	51.351	148	33	1	1	109	1	148	2.58e-44	143
MsG0180004296.01.T01	AT5G37780	47.853	163	31	2	1	109	1	163	3.66e-42	136
MsG0180004296.01.T01	AT2G41110	48.125	160	32	2	1	109	1	160	4.70e-42	136
MsG0180004296.01.T01	AT4G14640	71.277	94	24	1	20	110	39	132	5.47e-41	132
MsG0180004296.01.T01	AT5G37780	45.087	173	30	2	1	108	1	173	3.23e-40	132
MsG0180004296.01.T01	AT1G66410	46.835	158	35	2	1	109	1	158	1.11e-38	127
MsG0180004296.01.T01	AT1G66410	65.217	92	29	1	21	109	21	112	4.02e-38	124
MsG0180004296.01.T01	AT1G66410	65.217	92	29	1	21	109	21	112	4.02e-38	124
MsG0180004296.01.T01	AT3G43810	64.130	92	30	1	21	109	21	112	1.65e-37	123
MsG0180004296.01.T01	AT3G43810	64.130	92	30	1	21	109	21	112	1.65e-37	123
MsG0180004296.01.T01	AT5G37780	65.217	92	29	1	21	109	44	135	2.02e-37	123
MsG0180004296.01.T01	AT2G41090	35.417	144	57	1	1	108	1	144	4.55e-25	93.6
MsG0180004296.01.T01	AT1G66400	50.000	94	42	1	20	108	59	152	1.77e-21	83.6
MsG0180004296.01.T01	AT4G14640	46.154	104	17	1	4	68	5	108	2.87e-21	82.0
MsG0180004296.01.T01	AT3G51920	37.162	148	54	2	1	109	1	148	2.84e-20	80.5
MsG0180004296.01.T01	AT3G22930	44.231	104	19	1	5	69	28	131	4.08e-20	79.3
MsG0180004296.01.T01	AT3G22930	43.548	62	35	0	46	107	35	96	1.13e-11	57.8
MsG0180004296.01.T01	AT1G18210	47.126	87	45	1	21	107	68	153	9.32e-19	77.0
MsG0180004296.01.T01	AT1G18210	47.126	87	45	1	21	107	68	153	9.32e-19	77.0
MsG0180004296.01.T01	AT3G03000	32.886	149	52	3	5	109	13	157	1.18e-18	76.6
MsG0180004296.01.T01	AT1G18530	41.304	92	49	1	21	107	52	143	1.44e-18	76.3
MsG0180004296.01.T01	AT5G37770	36.691	139	45	2	12	107	17	155	3.48e-18	75.1
MsG0180004296.01.T01	AT5G37770	36.691	139	45	2	12	107	17	155	3.48e-18	75.1
MsG0180004296.01.T01	AT1G32250	32.051	156	57	3	1	107	1	156	3.49e-18	75.5
MsG0180004296.01.T01	AT1G62820	29.577	142	63	2	5	109	6	147	3.66e-18	74.7
MsG0180004296.01.T01	AT1G12310	29.577	142	63	2	5	109	6	147	4.35e-18	74.7
MsG0180004296.01.T01	AT3G50360	32.639	144	58	2	3	107	18	161	4.76e-18	75.1
MsG0180004296.01.T01	AT2G41100	34.356	163	51	4	1	107	55	217	5.22e-18	77.0
MsG0180004296.01.T01	AT2G41100	34.356	163	51	4	1	107	55	217	5.22e-18	77.0
MsG0180004296.01.T01	AT2G41100	34.356	163	51	4	1	107	55	217	5.22e-18	77.0
MsG0180004296.01.T01	AT2G41100	34.356	163	51	4	1	107	90	252	8.21e-18	77.0
MsG0180004296.01.T01	AT2G41100	43.548	62	35	0	46	107	12	73	3.85e-12	61.2
MsG0180004296.01.T01	AT2G41100	34.356	163	51	4	1	107	90	252	8.21e-18	77.0
MsG0180004296.01.T01	AT2G41100	43.548	62	35	0	46	107	12	73	3.85e-12	61.2
MsG0180004296.01.T01	AT1G24620	40.708	113	60	3	1	110	65	173	1.63e-17	73.9
MsG0180004296.01.T01	AT2G41100	53.521	71	32	1	38	107	57	127	5.37e-17	73.2
MsG0180004296.01.T01	AT1G73630	45.455	88	47	1	20	107	64	150	1.49e-16	71.2
MsG0180004296.01.T01	AT2G41100	51.613	62	30	0	46	107	12	73	2.62e-16	70.1
MsG0180004296.01.T01	AT4G37010	40.000	90	51	1	21	107	76	165	1.10e-15	68.9
MsG0180004296.01.T01	AT4G37010	43.750	64	36	0	46	109	31	94	3.54e-11	57.4
MsG0180004296.01.T01	AT4G37010	40.000	90	51	1	21	107	72	161	1.10e-15	68.9
MsG0180004296.01.T01	AT4G37010	43.750	64	36	0	46	109	27	90	3.32e-11	57.4
MsG0180004296.01.T01	AT3G25600	36.559	93	54	1	20	107	56	148	1.65e-15	68.2
MsG0180004296.01.T01	AT2G15680	38.095	105	62	2	7	109	81	184	3.93e-15	67.8
MsG0180004296.01.T01	AT4G38230	34.483	145	55	3	1	107	174	316	1.24e-14	68.6
MsG0180004296.01.T01	AT4G38230	34.483	145	55	3	1	107	348	490	1.76e-14	68.2
MsG0180004296.01.T01	AT4G38230	34.483	145	55	3	1	107	318	460	2.03e-14	68.2
MsG0180004296.01.T01	AT4G38230	34.483	145	55	3	1	107	318	460	2.03e-14	68.2
MsG0180004296.01.T01	AT2G43290	35.849	106	58	3	12	107	101	206	2.12e-14	66.6
MsG0180004296.01.T01	AT3G59440	34.286	140	52	3	8	107	47	186	2.76e-14	65.9
MsG0180004296.01.T01	AT2G41410	43.820	89	45	3	26	109	121	209	1.55e-13	64.3
MsG0180004296.01.T01	AT4G03290	41.053	95	48	2	21	107	50	144	1.64e-13	63.2
MsG0180004296.01.T01	AT3G10190	42.991	107	53	4	5	107	103	205	2.47e-13	63.5
MsG0180004296.01.T01	AT5G23580	32.414	145	58	3	1	107	316	458	2.65e-13	65.1
MsG0180004296.01.T01	AT1G35670	33.103	145	57	3	1	107	320	462	2.87e-13	64.7
MsG0180004296.01.T01	AT4G09570	33.103	145	57	3	1	107	319	461	3.96e-13	64.3
MsG0180004296.01.T01	AT1G05990	38.298	94	50	3	21	107	50	142	5.95e-13	61.6
MsG0180004296.01.T01	AT1G05990	43.077	65	37	0	46	110	5	69	1.51e-11	57.8
MsG0180004296.01.T01	AT3G03400	35.849	106	58	3	1	100	33	134	8.08e-13	60.8
MsG0180004296.01.T01	AT3G50770	46.154	65	35	0	43	107	61	125	9.08e-13	62.0
MsG0180004296.01.T01	AT5G44460	49.315	73	35	1	40	110	105	177	9.29e-13	61.6
MsG0180004296.01.T01	AT4G23650	45.161	62	34	0	47	108	384	445	1.01e-12	63.2
MsG0180004296.01.T01	AT4G23650	31.293	147	58	5	1	107	372	515	2.04e-11	59.7
MsG0180004296.01.T01	AT5G12180	32.877	146	57	4	1	107	367	510	1.47e-12	62.8
MsG0180004296.01.T01	AT5G12180	36.471	85	43	1	26	110	369	442	4.15e-11	58.5
MsG0180004296.01.T01	AT2G17290	32.414	145	58	3	1	107	379	521	1.75e-12	62.8
MsG0180004296.01.T01	AT2G17290	32.414	145	58	3	1	107	379	521	1.75e-12	62.8
MsG0180004296.01.T01	AT5G19360	32.192	146	58	4	1	107	362	505	3.52e-12	61.6
MsG0180004296.01.T01	AT5G19360	36.471	85	43	1	26	110	364	437	4.85e-11	58.5
MsG0180004296.01.T01	AT1G76040	31.034	145	61	3	1	107	167	310	3.80e-12	61.2
MsG0180004296.01.T01	AT1G76040	31.034	145	61	3	1	107	167	310	3.80e-12	61.2
MsG0180004296.01.T01	AT4G20780	50.725	69	32	1	44	110	118	186	5.38e-12	59.7
MsG0180004296.01.T01	AT3G07490	40.860	93	48	3	21	107	50	141	6.00e-12	58.9
MsG0180004296.01.T01	AT1G76040	31.034	145	61	3	1	107	405	548	6.11e-12	61.2
MsG0180004296.01.T01	AT5G42380	46.774	62	33	0	46	107	49	110	8.16e-12	59.3
MsG0180004296.01.T01	AT3G03410	34.259	108	62	3	1	108	30	128	8.26e-12	58.2
MsG0180004296.01.T01	AT5G17470	40.625	96	52	1	17	107	43	138	8.97e-12	58.2
MsG0180004296.01.T01	AT3G10660	28.966	145	63	3	1	107	480	622	1.14e-11	60.5
MsG0180004296.01.T01	AT3G10660	28.966	145	63	3	1	107	480	622	1.14e-11	60.5
MsG0180004296.01.T01	AT5G04870	29.655	145	62	3	1	107	444	586	1.28e-11	60.1
MsG0180004296.01.T01	AT2G38910	30.345	145	61	3	1	107	428	570	1.56e-11	60.1
MsG0180004296.01.T01	AT2G36180	39.796	98	51	2	17	107	41	137	1.58e-11	57.8
MsG0180004296.01.T01	AT4G35310	30.345	145	61	3	1	107	391	533	2.29e-11	59.3
MsG0180004296.01.T01	AT4G35310	30.345	145	61	3	1	107	391	533	2.29e-11	59.3
MsG0180004296.01.T01	AT1G74740	37.778	90	52	3	21	107	409	497	3.20e-11	58.9
MsG0180004296.01.T01	AT4G04700	41.935	93	47	4	21	109	381	470	4.15e-11	58.5
MsG0180004296.01.T01	AT4G04700	43.548	62	35	0	47	108	337	398	8.27e-11	57.8
MsG0180004296.01.T01	AT4G04720	38.532	109	57	5	5	109	416	518	4.32e-11	58.5
MsG0180004296.01.T01	AT4G04720	38.532	109	57	5	5	109	416	518	4.32e-11	58.5
MsG0180004296.01.T01	AT4G12860	38.384	99	54	3	15	107	44	141	8.42e-11	55.8

Find 19 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATTAGCCACTGTTATC+AGG	0.375137	1:+76231469	MsG0180004296.01.T01:CDS
TTTAGTTTGTTTGACAAAGA+TGG	0.393112	1:+76231315	MsG0180004296.01.T01:CDS
GATGATTAAAGAAGCAGATA+TGG	0.394958	1:+76232585	MsG0180004296.01.T01:CDS
GGAGCTTAGAGAGGCTTTCA+AGG	0.423469	1:+76231793	MsG0180004296.01.T01:CDS
GTCTTTGACAAAGATCAAAA+TGG	0.424272	1:+76231815	MsG0180004296.01.T01:CDS
CAACAATTTGTTCTTCACTA+AGG	0.446590	1:-76231278	None:intergenic
ATCTTTGTCAAACAAACTAA+AGG	0.531026	1:-76231313	None:intergenic
AGACACGTGATGATCAATCT+AGG	0.540494	1:+76232532	MsG0180004296.01.T01:CDS
ACAAATTGTTGATTTCAAAG+AGG	0.544777	1:+76231290	MsG0180004296.01.T01:CDS
CATTTAGGACACTGATGCAG+AGG	0.570696	1:+76231769	MsG0180004296.01.T01:intron
TTGTTTGACAAAGATGGAGA+TGG	0.596377	1:+76231321	MsG0180004296.01.T01:CDS
TTCACTAAGGACATGTGCCA+TGG	0.599624	1:-76231265	None:intergenic
ATTAAAGAAGCAGATATGGA+TGG	0.602493	1:+76232589	MsG0180004296.01.T01:CDS
GAAGCAGATATGGATGGTGA+TGG	0.608277	1:+76232595	MsG0180004296.01.T01:CDS
TTAGGACACTGATGCAGAGG+AGG	0.624491	1:+76231772	MsG0180004296.01.T01:intron
TGCAGAGGAGGAGCTTAGAG+AGG	0.630033	1:+76231784	MsG0180004296.01.T01:CDS
TGGCTATATATCAGCCAGTG+AGG	0.688712	1:+76231835	MsG0180004296.01.T01:CDS
GCAAACAGGTTGCATTACTG+TGG	0.701892	1:+76231442	MsG0180004296.01.T01:intron
ATCCAATGACCTGATAACAG+TGG	0.720716	1:-76231478	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATAAATAATGAATAGAA+TGG	-	Chr1:76231639-76231658	None:intergenic	10.0%
!!!	AAAATTTTAGAAAATCTATA+TGG	-	Chr1:76231923-76231942	None:intergenic	10.0%
!!!	AAATTTTAGAAAATCTATAT+GGG	-	Chr1:76231922-76231941	None:intergenic	10.0%
!!!	TCTAAAATTTTATTTTTTGA+TGG	+	Chr1:76231932-76231951	MsG0180004296.01.T01:intron	10.0%
!!	AAAAAGAAAAACTATGTAAA+TGG	-	Chr1:76232062-76232081	None:intergenic	15.0%
!!	TTATTAAGGTGAAAAAAAAA+TGG	-	Chr1:76232039-76232058	None:intergenic	15.0%
!!!	CTGTTATTTTATTTTCATTT+AGG	+	Chr1:76231754-76231773	MsG0180004296.01.T01:intron	15.0%
!!	AACTATGTAAATGGTTATTA+AGG	-	Chr1:76232053-76232072	None:intergenic	20.0%
!!	ATAGAAATTAAGATAGCAAT+GGG	+	Chr1:76232373-76232392	MsG0180004296.01.T01:intron	20.0%
!!	TAAATAATGAATAGAATGGA+AGG	-	Chr1:76231635-76231654	None:intergenic	20.0%
!	ATATCTTGTATTGTACATGA+AGG	+	Chr1:76232441-76232460	MsG0180004296.01.T01:intron	25.0%
!	ATCTTTGTCAAACAAACTAA+AGG	-	Chr1:76231316-76231335	None:intergenic	25.0%
!	CTTTCAATATCAAAGTTCTA+AGG	+	Chr1:76231669-76231688	MsG0180004296.01.T01:intron	25.0%
!	GATAGAAATTAAGATAGCAA+TGG	+	Chr1:76232372-76232391	MsG0180004296.01.T01:intron	25.0%
!	GATGGTAAAAAATTAATGTG+TGG	+	Chr1:76232496-76232515	MsG0180004296.01.T01:intron	25.0%
!	TACAATACAAGATATATACG+TGG	-	Chr1:76232436-76232455	None:intergenic	25.0%
!!	AACAATTTTATATACCTCAC+TGG	-	Chr1:76231852-76231871	None:intergenic	25.0%
!!	ATATTTTCATTTCCTCTGTT+TGG	+	Chr1:76232192-76232211	MsG0180004296.01.T01:intron	25.0%
!!	TATTTTCATTTCCTCTGTTT+GGG	+	Chr1:76232193-76232212	MsG0180004296.01.T01:intron	25.0%
!!!	ACAAATTGTTGATTTCAAAG+AGG	+	Chr1:76231290-76231309	MsG0180004296.01.T01:CDS	25.0%
!!!	TTTAGTTTGTTTGACAAAGA+TGG	+	Chr1:76231315-76231334	MsG0180004296.01.T01:CDS	25.0%
	ACAAGATATATACGTGGAAA+AGG	-	Chr1:76232430-76232449	None:intergenic	30.0%
	AGTGATGAGAATTCTGTTAA+AGG	-	Chr1:76231360-76231379	None:intergenic	30.0%
	ATTAAAGAAGCAGATATGGA+TGG	+	Chr1:76232589-76232608	MsG0180004296.01.T01:CDS	30.0%
	CAAGAACTCATCAAATTCAA+TGG	-	Chr1:76231568-76231587	None:intergenic	30.0%
	CAATAACCAAAGACTAAGTT+TGG	+	Chr1:76231729-76231748	MsG0180004296.01.T01:intron	30.0%
	GACAATGCATAATATATGCA+AGG	-	Chr1:76231978-76231997	None:intergenic	30.0%
	GATGATTAAAGAAGCAGATA+TGG	+	Chr1:76232585-76232604	MsG0180004296.01.T01:CDS	30.0%
	GTCTTTGACAAAGATCAAAA+TGG	+	Chr1:76231815-76231834	MsG0180004296.01.T01:CDS	30.0%
	TGAAAAACTAAGTGATGAAG+AGG	+	Chr1:76232555-76232574	MsG0180004296.01.T01:CDS	30.0%
	TTGGATAGTGAAATATACGA+TGG	+	Chr1:76232478-76232497	MsG0180004296.01.T01:intron	30.0%
!	CAACAATTTGTTCTTCACTA+AGG	-	Chr1:76231281-76231300	None:intergenic	30.0%
!!	ATCAAAGTTCTAAGGTCTAT+AGG	+	Chr1:76231677-76231696	MsG0180004296.01.T01:intron	30.0%
	AAGAATTAGCCACTGTTATC+AGG	+	Chr1:76231469-76231488	MsG0180004296.01.T01:CDS	35.0%
	AATCTTGTGAATGTGCAAAC+AGG	+	Chr1:76231428-76231447	MsG0180004296.01.T01:intron	35.0%
	AATGATTACTTCCCAAACAG+AGG	-	Chr1:76232207-76232226	None:intergenic	35.0%
	CATAATATATGCAAGGTCTG+GGG	-	Chr1:76231971-76231990	None:intergenic	35.0%
	CATATCTTGTAGCTCTTCTT+CGG	-	Chr1:76231514-76231533	None:intergenic	35.0%
	GCATAATATATGCAAGGTCT+GGG	-	Chr1:76231972-76231991	None:intergenic	35.0%
	TCATACAGCATCATATGCAT+AGG	+	Chr1:76231381-76231400	MsG0180004296.01.T01:intron	35.0%
	TGATGAGTTCTTGAACTTGA+TGG	+	Chr1:76231575-76231594	MsG0180004296.01.T01:intron	35.0%
	TGCATAATATATGCAAGGTC+TGG	-	Chr1:76231973-76231992	None:intergenic	35.0%
	TTATATCCACATGCACATCT+AGG	+	Chr1:76232297-76232316	MsG0180004296.01.T01:intron	35.0%
	TTGTTTGACAAAGATGGAGA+TGG	+	Chr1:76231321-76231340	MsG0180004296.01.T01:CDS	35.0%
!	ACAGAACCAAACTTAGTCTT+TGG	-	Chr1:76231738-76231757	None:intergenic	35.0%
!	GAAGGCATAAACATGCATAT+TGG	+	Chr1:76232459-76232478	MsG0180004296.01.T01:intron	35.0%
!	TGAGTTTAGTTCAGTTGGTA+AGG	-	Chr1:76232000-76232019	None:intergenic	35.0%
!!	ATAAGTGAAGTTGATGCAGA+TGG	+	Chr1:76231534-76231553	MsG0180004296.01.T01:intron	35.0%
	AGACACGTGATGATCAATCT+AGG	+	Chr1:76232532-76232551	MsG0180004296.01.T01:CDS	40.0%
	ATCCAATGACCTGATAACAG+TGG	-	Chr1:76231481-76231500	None:intergenic	40.0%
	CAGCTAATATTCACTGCATG+TGG	-	Chr1:76232341-76232360	None:intergenic	40.0%
	CCAACTGAACTAAACTCACA+AGG	+	Chr1:76232002-76232021	MsG0180004296.01.T01:intron	40.0%
!	AGTTCTTGAACTTGATGGCT+AGG	+	Chr1:76231580-76231599	MsG0180004296.01.T01:intron	40.0%
!	CCTTGTGAGTTTAGTTCAGT+TGG	-	Chr1:76232005-76232024	None:intergenic	40.0%
	AGCCACTGTTATCAGGTCAT+TGG	+	Chr1:76231476-76231495	MsG0180004296.01.T01:intron	45.0%
	ATCTTGTAGCTCTTCTTCGG+TGG	-	Chr1:76231511-76231530	None:intergenic	45.0%
	GAAGCAGATATGGATGGTGA+TGG	+	Chr1:76232595-76232614	MsG0180004296.01.T01:CDS	45.0%
	GCAAACAGGTTGCATTACTG+TGG	+	Chr1:76231442-76231461	MsG0180004296.01.T01:intron	45.0%
	TACTGTCCTAGATGTGCATG+TGG	-	Chr1:76232306-76232325	None:intergenic	45.0%
	TCACTGCATGTGGAAACGTA+CGG	-	Chr1:76232331-76232350	None:intergenic	45.0%
	TCTTGTAGCTCTTCTTCGGT+GGG	-	Chr1:76231510-76231529	None:intergenic	45.0%
	TGGCTATATATCAGCCAGTG+AGG	+	Chr1:76231835-76231854	MsG0180004296.01.T01:CDS	45.0%
!	CATTTAGGACACTGATGCAG+AGG	+	Chr1:76231769-76231788	MsG0180004296.01.T01:intron	45.0%
!!	GAAGTTGATGCAGATGGCAA+TGG	+	Chr1:76231540-76231559	MsG0180004296.01.T01:intron	45.0%
!!!	TTTAAAATTTGTATTAAAAA+TGG	-	Chr1:76232095-76232114	None:intergenic	5.0%
	GGAGCTTAGAGAGGCTTTCA+AGG	+	Chr1:76231793-76231812	MsG0180004296.01.T01:CDS	50.0%
	TTAGGACACTGATGCAGAGG+AGG	+	Chr1:76231772-76231791	MsG0180004296.01.T01:intron	50.0%
	TGCAGAGGAGGAGCTTAGAG+AGG	+	Chr1:76231784-76231803	MsG0180004296.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	76231267	76232666	76231267	ID=MsG0180004296.01;Name=MsG0180004296.01
Chr1	mRNA	76231267	76232666	76231267	ID=MsG0180004296.01.T01;Parent=MsG0180004296.01;Name=MsG0180004296.01.T01;_AED=0.42;_eAED=0.46;_QI=0\|0\|0\|1\|0.33\|0.5\|4\|0\|111
Chr1	exon	76231267	76231342	76231267	ID=MsG0180004296.01.T01:exon:12592;Parent=MsG0180004296.01.T01
Chr1	exon	76231450	76231490	76231450	ID=MsG0180004296.01.T01:exon:12593;Parent=MsG0180004296.01.T01
Chr1	exon	76231776	76231856	76231776	ID=MsG0180004296.01.T01:exon:12594;Parent=MsG0180004296.01.T01
Chr1	exon	76232529	76232666	76232529	ID=MsG0180004296.01.T01:exon:12595;Parent=MsG0180004296.01.T01
Chr1	CDS	76231267	76231342	76231267	ID=MsG0180004296.01.T01:cds;Parent=MsG0180004296.01.T01
Chr1	CDS	76231450	76231490	76231450	ID=MsG0180004296.01.T01:cds;Parent=MsG0180004296.01.T01
Chr1	CDS	76231776	76231856	76231776	ID=MsG0180004296.01.T01:cds;Parent=MsG0180004296.01.T01
Chr1	CDS	76232529	76232666	76232529	ID=MsG0180004296.01.T01:cds;Parent=MsG0180004296.01.T01

Gene Sequence	>MsG0180004296.01.T01 ATGGCACATGTCCTTAGTGAAGAACAAATTGTTGATTTCAAAGAGGCCTTTAGTTTGTTTGACAAAGATGGAGATGGTTGCATTACTGTGGAAGAAAGAATTAGCCACTGTTATCAGGACACTGATGCAGAGGAGGAGCTTAGAGAGGCTTTCAAGGTCTTTGACAAAGATCAAAATGGCTATATATCAGCCAGTGAGTTGAGACACGTGATGATCAATCTAGGTGAAAAACTAAGTGATGAAGAGGTAGAGCAGATGATTAAAGAAGCAGATATGGATGGTGATGGTCAAGTTGACTTTGATGAATTCGTCAAGATGATGATGACCATTCGATGA
Protein sequence	>MsG0180004296.01.T01 MAHVLSEEQIVDFKEAFSLFDKDGDGCITVEERISHCYQDTDAEEELREAFKVFDKDQNGYISASELRHVMINLGEKLSDEEVEQMIKEADMDGDGQVDFDEFVKMMMTIR*