AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018807.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018807.01.T01 MTR_4g018880 98.333 180 3 0 1 180 1 180 5.39e-129 359
MsG0480018807.01.T01 MTR_4g018860 84.024 169 27 0 1 169 1 169 5.31e-105 300
MsG0480018807.01.T01 MTR_4g018850 81.564 179 33 0 1 179 1 179 9.38e-101 289
MsG0480018807.01.T01 MTR_4g018880 97.727 132 3 0 1 132 1 132 2.01e-92 265
MsG0480018807.01.T01 MTR_7g066110 68.452 168 53 0 11 178 1 168 4.63e-85 248
MsG0480018807.01.T01 MTR_6g452730 52.201 159 74 2 9 165 5 163 5.62e-57 177
MsG0480018807.01.T01 MTR_1g101120 54.248 153 69 1 15 166 18 170 7.63e-57 177
MsG0480018807.01.T01 MTR_7g066090 63.158 76 28 0 1 76 1 76 2.59e-29 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018807.01.T01 AT3G54040 54.011 187 76 5 1 181 1 183 1.03e-63 194
MsG0480018807.01.T01 AT3G54040 54.011 187 76 5 1 181 57 239 3.15e-63 195
MsG0480018807.01.T01 AT5G52390 43.956 182 93 2 7 179 1 182 1.54e-50 161

Find 54 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTCTTCTCAGTAATTT+TGG 0.107847 4:-10151907 MsG0480018807.01.T01:exon
GTTCTCCGATTATCAAATTT+TGG 0.174880 4:-10151701 MsG0480018807.01.T01:three_prime_UTR
AAAGGTATAGTTTGGGAAAA+TGG 0.222736 4:+10153445 None:intergenic
AATATATAAATTTAATGTTT+TGG 0.227941 4:+10151866 None:intergenic
TTCTAAATAAAGGTATAGTT+TGG 0.253324 4:+10153437 None:intergenic
TTTCTCATCCATGCATCTTT+AGG 0.256802 4:-10153367 MsG0480018807.01.T01:intron
AAAATGGAGAAGTTGTATTT+AGG 0.267495 4:+10153461 None:intergenic
AAGAAAAGGTCAACAATGTT+AGG 0.281319 4:+10152922 None:intergenic
TTGTGTTTCTCACATAGTTC+TGG 0.298056 4:+10152031 None:intergenic
TTTGGAATTGTTTCTACTCT+TGG 0.333913 4:-10151683 MsG0480018807.01.T01:three_prime_UTR
TTTCTCAAGCAAACTATCAT+TGG 0.351663 4:-10153407 MsG0480018807.01.T01:CDS
AAAGACATGGTTCTAAATAA+AGG 0.352345 4:+10153427 None:intergenic
CAATTCCAAAATTTGATAAT+CGG 0.354491 4:+10151696 None:intergenic
TGCATTTGGTCCCGCTTCTT+CGG 0.355225 4:-10151963 MsG0480018807.01.T01:CDS
GTGAGCTCCGGAGCTGCATT+TGG 0.357959 4:-10151977 MsG0480018807.01.T01:CDS
ATCTGGAAAGAGATGTTTGT+TGG 0.389390 4:-10153151 MsG0480018807.01.T01:CDS
GAAGTTGTATTTAGGAAAGA+AGG 0.394771 4:+10153469 None:intergenic
TCTAAATAAAGGTATAGTTT+GGG 0.400681 4:+10153438 None:intergenic
TGTTTGTTGGAGACTCAAAA+AGG 0.420286 4:-10153138 MsG0480018807.01.T01:CDS
ACTTTCTCAGTAGCTTCATC+TGG 0.424996 4:-10153168 MsG0480018807.01.T01:CDS
GAGAAAGTACACACTTCCTT+TGG 0.445012 4:+10153183 None:intergenic
AGCGGGACCAAATGCAGCTC+CGG 0.458285 4:+10151970 None:intergenic
CAGCAGCCATATTGAAGAAA+AGG 0.460023 4:+10152908 None:intergenic
CTCCAAAATTACTGAGAAGA+AGG 0.460063 4:+10151905 None:intergenic
TTCAATATGGCTGCTGGAGA+GGG 0.471243 4:-10152901 MsG0480018807.01.T01:intron
TTCTTTGAACCACAATTCAC+TGG 0.475595 4:-10152979 MsG0480018807.01.T01:CDS
GTAATAATATCAAGTTGAGC+TGG 0.478219 4:-10151768 MsG0480018807.01.T01:three_prime_UTR
CTATAGTAGAGGATATATAG+AGG 0.488277 4:-10151833 MsG0480018807.01.T01:three_prime_UTR
TAGAACATCTGAAGTTATAG+TGG 0.488747 4:-10153088 MsG0480018807.01.T01:CDS
CTTCAATATGGCTGCTGGAG+AGG 0.505716 4:-10152902 MsG0480018807.01.T01:CDS
CAAAGTTTGCCAGTGAATTG+TGG 0.513259 4:+10152970 None:intergenic
ATGGTCGAGCTTGTGAGCTC+CGG 0.514705 4:-10151989 MsG0480018807.01.T01:CDS
AGAAAAGGTCAACAATGTTA+GGG 0.524892 4:+10152923 None:intergenic
TGAGAAGAAGGTGCAGCTGC+AGG 0.526134 4:+10151917 None:intergenic
GACCAACCCTCGTCGTGCTA+TGG 0.530876 4:-10152008 MsG0480018807.01.T01:CDS
TCCTCTGAAACCGAAGAAGC+GGG 0.533162 4:+10151953 None:intergenic
GTCCTCTGAAACCGAAGAAG+CGG 0.562733 4:+10151952 None:intergenic
TCCCGCTTCTTCGGTTTCAG+AGG 0.566231 4:-10151954 MsG0480018807.01.T01:CDS
TCCTATGTTTACTATAGTAG+AGG 0.567624 4:-10151844 MsG0480018807.01.T01:three_prime_UTR
TATAGTAGAGGATATATAGA+GGG 0.575325 4:-10151832 MsG0480018807.01.T01:three_prime_UTR
GACCATAGCACGACGAGGGT+TGG 0.575610 4:+10152006 None:intergenic
TAATAATATCAAGTTGAGCT+GGG 0.579142 4:-10151767 MsG0480018807.01.T01:three_prime_UTR
TGCAGCTGAAATGGCATGTG+AGG 0.589265 4:-10153211 MsG0480018807.01.T01:intron
TATACATACCTAAAGATGCA+TGG 0.589615 4:+10153359 None:intergenic
TCCTCTACTATAGTAAACAT+AGG 0.591635 4:+10151843 None:intergenic
CACCACATGCTTCCACACAT+TGG 0.599473 4:+10153037 None:intergenic
GGTGTTGATAGAAGTTCTGT+TGG 0.610517 4:-10153018 MsG0480018807.01.T01:CDS
TAGTTTGCTTGAGAAAGACA+TGG 0.639175 4:+10153414 None:intergenic
TCAAAACAAATTCAATAACA+AGG 0.649748 4:+10151796 None:intergenic
GGCATGTGAGGACTTACCAA+AGG 0.656235 4:-10153199 MsG0480018807.01.T01:CDS
AGCTCGACCATAGCACGACG+AGG 0.664011 4:+10152001 None:intergenic
CATTGAGACAGACCAATGTG+TGG 0.673666 4:-10153049 MsG0480018807.01.T01:CDS
GCTCGACCATAGCACGACGA+GGG 0.698174 4:+10152002 None:intergenic
GACCAATGTGTGGAAGCATG+TGG 0.721045 4:-10153039 MsG0480018807.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATATAATTATATGTGT+TGG - Chr4:10152476-10152495 MsG0480018807.01.T01:intron 10.0%
!!! TTTGAAGATAAAATATTTTA+TGG + Chr4:10152932-10152951 None:intergenic 10.0%
!! ATTAAAAAAAAATGTAACCT+AGG - Chr4:10153001-10153020 MsG0480018807.01.T01:CDS 15.0%
!!! GATTTTTTTCAAATTATTAG+TGG + Chr4:10152450-10152469 None:intergenic 15.0%
!! ATTGTAGATATTGGAAAATT+TGG + Chr4:10152698-10152717 None:intergenic 20.0%
!! TAGAAAATAAACAAATTCCT+AGG + Chr4:10153021-10153040 None:intergenic 20.0%
!! TCAAAACAAATTCAATAACA+AGG + Chr4:10153387-10153406 None:intergenic 20.0%
!! TTTATCTTCAAATCTAATCA+AGG - Chr4:10152940-10152959 MsG0480018807.01.T01:CDS 20.0%
!!! CAATTCCAAAATTTGATAAT+CGG + Chr4:10153487-10153506 None:intergenic 20.0%
!!! TCTAAATAAAGGTATAGTTT+GGG + Chr4:10151745-10151764 None:intergenic 20.0%
!!! TTAGTTGTTCATTTTTTTCT+TGG - Chr4:10151902-10151921 MsG0480018807.01.T01:exon 20.0%
!!! TTCTAAATAAAGGTATAGTT+TGG + Chr4:10151746-10151765 None:intergenic 20.0%
!!! TTTTCCAATATCTACAATTT+TGG - Chr4:10152700-10152719 MsG0480018807.01.T01:intron 20.0%
! AAAATGGAGAAGTTGTATTT+AGG + Chr4:10151722-10151741 None:intergenic 25.0%
! AGTTGTGTCTAAATTACAAT+AGG - Chr4:10152357-10152376 MsG0480018807.01.T01:intron 25.0%
! ATGCCAATTAACAAAACATA+AGG + Chr4:10153083-10153102 None:intergenic 25.0%
! TATAGTAGAGGATATATAGA+GGG - Chr4:10153348-10153367 MsG0480018807.01.T01:intron 25.0%
! TTCTTTAATTCTTCTATGCA+AGG - Chr4:10152781-10152800 MsG0480018807.01.T01:intron 25.0%
!! TAAACATTGCTCTAAATGTA+AGG + Chr4:10152853-10152872 None:intergenic 25.0%
!! TTAAACTATTCTAGAGAAAC+CGG + Chr4:10152585-10152604 None:intergenic 25.0%
!!! AAAGACATGGTTCTAAATAA+AGG + Chr4:10151756-10151775 None:intergenic 25.0%
!!! TAATAATATCAAGTTGAGCT+GGG - Chr4:10153413-10153432 MsG0480018807.01.T01:CDS 25.0%
!!! TCTCCTTATGTTTTGTTAAT+TGG - Chr4:10153077-10153096 MsG0480018807.01.T01:CDS 25.0%
AAGAAAAGGTCAACAATGTT+AGG + Chr4:10152261-10152280 None:intergenic 30.0%
AGAAAAGGTCAACAATGTTA+GGG + Chr4:10152260-10152279 None:intergenic 30.0%
CCTGTTGTCACATAAATTTA+AGG + Chr4:10153119-10153138 None:intergenic 30.0%
CCTTAAATTTATGTGACAAC+AGG - Chr4:10153116-10153135 MsG0480018807.01.T01:CDS 30.0%
CTATAGTAGAGGATATATAG+AGG - Chr4:10153347-10153366 MsG0480018807.01.T01:intron 30.0%
GAAGTTGTATTTAGGAAAGA+AGG + Chr4:10151714-10151733 None:intergenic 30.0%
TATACATACCTAAAGATGCA+TGG + Chr4:10151824-10151843 None:intergenic 30.0%
TCCTATGTTTACTATAGTAG+AGG - Chr4:10153336-10153355 MsG0480018807.01.T01:intron 30.0%
TCCTCTACTATAGTAAACAT+AGG + Chr4:10153340-10153359 None:intergenic 30.0%
TTTCTCAAGCAAACTATCAT+TGG - Chr4:10151773-10151792 MsG0480018807.01.T01:three_prime_UTR 30.0%
! AAAGGTATAGTTTGGGAAAA+TGG + Chr4:10151738-10151757 None:intergenic 30.0%
! ACAAATTTTTGCAGCTGAAA+TGG - Chr4:10151960-10151979 MsG0480018807.01.T01:CDS 30.0%
! ATTGCTCTAAATGTAAGGAA+AGG + Chr4:10152848-10152867 None:intergenic 30.0%
! GTTCTCCGATTATCAAATTT+TGG - Chr4:10153479-10153498 MsG0480018807.01.T01:five_prime_UTR 30.0%
! TAGAACATCTGAAGTTATAG+TGG - Chr4:10152092-10152111 MsG0480018807.01.T01:intron 30.0%
! TCCAATATCTACAATTTTGG+AGG - Chr4:10152703-10152722 MsG0480018807.01.T01:intron 30.0%
! TGTTGACCTTTTCTTCAATA+TGG - Chr4:10152266-10152285 MsG0480018807.01.T01:intron 30.0%
!! GTAATAATATCAAGTTGAGC+TGG - Chr4:10153412-10153431 MsG0480018807.01.T01:CDS 30.0%
!! TTTGGAATTGTTTCTACTCT+TGG - Chr4:10153497-10153516 MsG0480018807.01.T01:five_prime_UTR 30.0%
ACTTTCTTAGTGATTGTCGA+AGG + Chr4:10152900-10152919 None:intergenic 35.0%
ATCTGGAAAGAGATGTTTGT+TGG - Chr4:10152029-10152048 MsG0480018807.01.T01:CDS 35.0%
CTCCAAAATTACTGAGAAGA+AGG + Chr4:10153278-10153297 None:intergenic 35.0%
GCCTCCAAAATTGTAGATAT+TGG + Chr4:10152707-10152726 None:intergenic 35.0%
GTCAATATCTGTGTCATATC+AGG + Chr4:10152418-10152437 None:intergenic 35.0%
TATGAAGCATAGACACTCAT+CGG + Chr4:10152554-10152573 None:intergenic 35.0%
TTCTTTGAACCACAATTCAC+TGG - Chr4:10152201-10152220 MsG0480018807.01.T01:intron 35.0%
TTGTGTTTCTCACATAGTTC+TGG + Chr4:10153152-10153171 None:intergenic 35.0%
TTTCTCATCCATGCATCTTT+AGG - Chr4:10151813-10151832 MsG0480018807.01.T01:three_prime_UTR 35.0%
! CACCTTCTTCTCAGTAATTT+TGG - Chr4:10153273-10153292 MsG0480018807.01.T01:intron 35.0%
! TGTTTGTTGGAGACTCAAAA+AGG - Chr4:10152042-10152061 MsG0480018807.01.T01:CDS 35.0%
!! TAGTTTGCTTGAGAAAGACA+TGG + Chr4:10151769-10151788 None:intergenic 35.0%
!! TCTCAGTAATTTTGGAGAGA+TGG - Chr4:10153281-10153300 MsG0480018807.01.T01:intron 35.0%
ACTTTCTCAGTAGCTTCATC+TGG - Chr4:10152012-10152031 MsG0480018807.01.T01:CDS 40.0%
CAAAGTTTGCCAGTGAATTG+TGG + Chr4:10152213-10152232 None:intergenic 40.0%
CAGCAGCCATATTGAAGAAA+AGG + Chr4:10152275-10152294 None:intergenic 40.0%
GATTAGATGTGTCCTGATGT+TGG + Chr4:10152532-10152551 None:intergenic 40.0%
TATGCTTCATAGCAACTCAC+CGG - Chr4:10152563-10152582 MsG0480018807.01.T01:intron 40.0%
! CTTTTCTTCAATATGGCTGC+TGG - Chr4:10152273-10152292 MsG0480018807.01.T01:intron 40.0%
! GAGAAAGTACACACTTCCTT+TGG + Chr4:10152000-10152019 None:intergenic 40.0%
!! GAGACTCAAAAAGGTGTCAA+TGG - Chr4:10152051-10152070 MsG0480018807.01.T01:intron 40.0%
!! GCACTTTTTGTAACAAGCTG+GGG + Chr4:10152238-10152257 None:intergenic 40.0%
!! GGCACTTTTTGTAACAAGCT+GGG + Chr4:10152239-10152258 None:intergenic 40.0%
!! GGTGTTGATAGAAGTTCTGT+TGG - Chr4:10152162-10152181 MsG0480018807.01.T01:intron 40.0%
CATTGAGACAGACCAATGTG+TGG - Chr4:10152131-10152150 MsG0480018807.01.T01:intron 45.0%
TCTTAGTGATTGTCGAAGGC+AGG + Chr4:10152896-10152915 None:intergenic 45.0%
TTCAATATGGCTGCTGGAGA+GGG - Chr4:10152279-10152298 MsG0480018807.01.T01:intron 45.0%
!! GGGCACTTTTTGTAACAAGC+TGG + Chr4:10152240-10152259 None:intergenic 45.0%
!!! AATATATAAATTTAATGTTT+TGG + Chr4:10153317-10153336 None:intergenic 5.0%
!!! ATATTGATTTTTTTTTTAAT+CGG + Chr4:10152620-10152639 None:intergenic 5.0%
CACCACATGCTTCCACACAT+TGG + Chr4:10152146-10152165 None:intergenic 50.0%
CTTCAATATGGCTGCTGGAG+AGG - Chr4:10152278-10152297 MsG0480018807.01.T01:intron 50.0%
GGCATGTGAGGACTTACCAA+AGG - Chr4:10151981-10152000 MsG0480018807.01.T01:CDS 50.0%
TGCAGCTGAAATGGCATGTG+AGG - Chr4:10151969-10151988 MsG0480018807.01.T01:CDS 50.0%
TGCATTTGGTCCCGCTTCTT+CGG - Chr4:10153217-10153236 MsG0480018807.01.T01:intron 50.0%
! GACCAATGTGTGGAAGCATG+TGG - Chr4:10152141-10152160 MsG0480018807.01.T01:intron 50.0%
!! GTCCTCTGAAACCGAAGAAG+CGG + Chr4:10153231-10153250 None:intergenic 50.0%
!! TCCTCTGAAACCGAAGAAGC+GGG + Chr4:10153230-10153249 None:intergenic 50.0%
ATGGTCGAGCTTGTGAGCTC+CGG - Chr4:10153191-10153210 MsG0480018807.01.T01:CDS 55.0%
CACGACACAGGTCCAACATC+AGG - Chr4:10152517-10152536 MsG0480018807.01.T01:intron 55.0%
GTCTGTGTCAGACACGACAC+AGG - Chr4:10152505-10152524 MsG0480018807.01.T01:intron 55.0%
TCCCGCTTCTTCGGTTTCAG+AGG - Chr4:10153226-10153245 MsG0480018807.01.T01:intron 55.0%
! TGAGAAGAAGGTGCAGCTGC+AGG + Chr4:10153266-10153285 None:intergenic 55.0%
AGCGGGACCAAATGCAGCTC+CGG + Chr4:10153213-10153232 None:intergenic 60.0%
AGCTCGACCATAGCACGACG+AGG + Chr4:10153182-10153201 None:intergenic 60.0%
GACCAACCCTCGTCGTGCTA+TGG - Chr4:10153172-10153191 MsG0480018807.01.T01:CDS 60.0%
GACCATAGCACGACGAGGGT+TGG + Chr4:10153177-10153196 None:intergenic 60.0%
GCTCGACCATAGCACGACGA+GGG + Chr4:10153181-10153200 None:intergenic 60.0%
GTGAGCTCCGGAGCTGCATT+TGG - Chr4:10153203-10153222 MsG0480018807.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 10151631 10153571 10151631 ID=MsG0480018807.01;Name=MsG0480018807.01
Chr4 mRNA 10151631 10153571 10151631 ID=MsG0480018807.01.T01;Parent=MsG0480018807.01;Name=MsG0480018807.01.T01;_AED=0.49;_eAED=0.49;_QI=137|1|1|1|0.5|0.66|3|282|181
Chr4 exon 10151631 10152064 10151631 ID=MsG0480018807.01.T01:exon:2862;Parent=MsG0480018807.01.T01
Chr4 exon 10152902 10153228 10152902 ID=MsG0480018807.01.T01:exon:2861;Parent=MsG0480018807.01.T01
Chr4 exon 10153368 10153571 10153368 ID=MsG0480018807.01.T01:exon:2860;Parent=MsG0480018807.01.T01
Chr4 five_prime_UTR 10153435 10153571 10153435 ID=MsG0480018807.01.T01:five_prime_utr;Parent=MsG0480018807.01.T01
Chr4 CDS 10153368 10153434 10153368 ID=MsG0480018807.01.T01:cds;Parent=MsG0480018807.01.T01
Chr4 CDS 10152902 10153228 10152902 ID=MsG0480018807.01.T01:cds;Parent=MsG0480018807.01.T01
Chr4 CDS 10151913 10152064 10151913 ID=MsG0480018807.01.T01:cds;Parent=MsG0480018807.01.T01
Chr4 three_prime_UTR 10151631 10151912 10151631 ID=MsG0480018807.01.T01:three_prime_utr;Parent=MsG0480018807.01.T01
Gene Sequence

>MsG0480018807.01.T01

ATGTCTTTCTCAAGCAAACTATCATTGGTTTTGTTATTTTCTTCTTTTCTCATCCATGCATCTTTAGCTGAAATGGCATGTGAGGACTTACCAAAGGAAGTGTGTACTTTCTCAGTAGCTTCATCTGGAAAGAGATGTTTGTTGGAGACTCAAAAAGGTGTCAATGGTGAAATTGAGTATCAATGTAGAACATCTGAAGTTATAGTGGAGAGAATATCAGAATACATTGAGACAGACCAATGTGTGGAAGCATGTGGTGTTGATAGAAGTTCTGTTGGTATTTCATCTGATGCTTTCTTTGAACCACAATTCACTGGCAAACTTTGCTCCCCAGCTTGTTACAAAAAGTGCCCTAACATTGTTGACCTTTTCTTCAATATGGCTGCTGGAGAGGGAGTATTTTTACCAGAACTATGTGAGAAACACAAGACCAACCCTCGTCGTGCTATGGTCGAGCTTGTGAGCTCCGGAGCTGCATTTGGTCCCGCTTCTTCGGTTTCAGAGGACATTGTGTTAGCACCTGCAGCTGCACCTTCTTCTCAGTAA

Protein sequence

>MsG0480018807.01.T01

MSFSSKLSLVLLFSSFLIHASLAEMACEDLPKEVCTFSVASSGKRCLLETQKGVNGEIEYQCRTSEVIVERISEYIETDQCVEACGVDRSSVGISSDAFFEPQFTGKLCSPACYKKCPNIVDLFFNMAAGEGVFLPELCEKHKTNPRRAMVELVSSGAAFGPASSVSEDIVLAPAAAPSSQ*