AlfalfaGEDB | MsG0180004311.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004311.01

KEGG: K06995
GO: -
PFAM: PF05899

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004311.01.T01	XP_013468793.1	96.241	133	3	1	3	135	2	132	4.65E-87	259

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004311.01.T01	A0A072VXN1	96.241	133	3	1	3	135	2	132	2.22e-87	259

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0180004311.01	0.856498	3.089368e-62	7.389555e-59
MsG0080047945.01	MsG0180004311.01	0.831814	1.348860e-55	1.480335e-52
MsG0080047955.01	MsG0180004311.01	0.856039	4.211478e-62	9.919167e-59
MsG0080047956.01	MsG0180004311.01	0.847270	1.286225e-59	2.273821e-56
MsG0080047989.01	MsG0180004311.01	0.876231	1.645454e-68	7.915152e-65
MsG0080048059.01	MsG0180004311.01	0.825550	4.409912e-54	4.032358e-51
MsG0080048068.01	MsG0180004311.01	0.826147	3.182650e-54	2.960080e-51
MsG0080048198.01	MsG0180004311.01	0.820047	8.432693e-53	6.600886e-50
MsG0080048256.01	MsG0180004311.01	-0.808274	3.356346e-50	1.910962e-47
MsG0080048273.01	MsG0180004311.01	0.866520	2.685794e-65	9.078770e-62

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004311.01.T01	MTR_1g076840	96.241	133	3	1	3	135	2	132	5.63e-91	259
MsG0180004311.01.T01	MTR_6g060170	82.474	97	17	0	39	135	2	98	1.77e-57	174
MsG0180004311.01.T01	MTR_1g069370	60.440	91	36	0	45	135	9	99	4.77e-38	125
MsG0180004311.01.T01	MTR_7g099200	59.341	91	37	0	45	135	5	95	2.80e-37	123
MsG0180004311.01.T01	MTR_6g059920	53.763	93	43	0	40	132	33	125	1.59e-30	106
MsG0180004311.01.T01	MTR_5g005820	51.087	92	45	0	40	131	2	93	2.54e-26	95.1
MsG0180004311.01.T01	MTR_6g060000	86.047	43	6	0	76	118	19	61	3.78e-22	83.6
MsG0180004311.01.T01	MTR_6g060090	68.000	50	14	1	68	117	9	56	2.28e-18	73.6
MsG0180004311.01.T01	MTR_1g086000	32.500	80	54	0	37	116	39	118	1.45e-12	61.2
MsG0180004311.01.T01	MTR_6g060030	55.172	58	8	1	96	135	7	64	1.68e-12	58.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004311.01.T01	AT4G10300	67.669	133	39	3	6	135	3	134	1.07e-59	181
MsG0180004311.01.T01	AT3G04300	56.044	91	40	0	45	135	1	91	1.72e-35	118
MsG0180004311.01.T01	AT4G28703	48.454	97	41	1	47	134	8	104	5.09e-29	102
MsG0180004311.01.T01	AT4G10280	48.454	97	47	1	42	135	43	139	1.60e-26	97.1
MsG0180004311.01.T01	AT4G10290	43.617	94	50	1	42	132	15	108	3.39e-20	80.1
MsG0180004311.01.T01	AT2G32180	30.380	79	55	0	41	119	40	118	1.02e-11	58.9
MsG0180004311.01.T01	AT2G32180	30.380	79	55	0	41	119	40	118	1.02e-11	58.9
MsG0180004311.01.T01	AT2G32650	30.380	79	55	0	41	119	40	118	1.12e-11	58.5
MsG0180004311.01.T01	AT2G32650	30.380	79	55	0	41	119	40	118	1.12e-11	58.5

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Find 35 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGATGAGTTGTACTT+GGG	0.609307	1:+76344076	MsG0180004311.01.T01:CDS
AAAGTGAAGGTCACTCCTAG+TGG	0.587646	1:+76344005	MsG0180004311.01.T01:CDS
AACACCAAGTTGAGTGAGCT+TGG	0.455326	1:-76343496	None:intergenic
AACAGAGACATGCTATCTTT+TGG	0.368243	1:+76343977	MsG0180004311.01.T01:CDS
AAGTGAAGGTCACTCCTAGT+GGG	0.564562	1:+76344006	MsG0180004311.01.T01:CDS
ACAACTCTTCTCCTTGTTGA+AGG	0.406999	1:-76343417	None:intergenic
ACTGCCTCATTAGCCCCACT+AGG	0.662701	1:-76344020	None:intergenic
AGCTCACTCAACTTGGTGTT+AGG	0.443465	1:+76343499	MsG0180004311.01.T01:CDS
AGGCAAAGTTACACTTACCT+TGG	0.501016	1:-76343397	None:intergenic
AGTCAAATGTTTGGTTTGTG+AGG	0.547845	1:-76343364	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGAAATGTGGTATTTACT+TGG	-	Chr1:76343532-76343551	None:intergenic	30.0%
GGTGTAAATAAAAACAGATG+GGG	+	Chr1:76343918-76343937	MsG0180004311.01.T01:intron	30.0%
TATGTCCATGGAAAATTACT+TGG	-	Chr1:76343951-76343970	None:intergenic	30.0%
AAAAGGGATGAGTTGTACTT+GGG	+	Chr1:76344076-76344095	MsG0180004311.01.T01:CDS	35.0%
TGAAATTTCTGCTGGTGATT+TGG	+	Chr1:76344043-76344062	MsG0180004311.01.T01:CDS	35.0%
ACAACTCTTCTCCTTGTTGA+AGG	-	Chr1:76343420-76343439	None:intergenic	40.0%
ACTAGAAGAGCAACTAGCAT+AGG	-	Chr1:76343696-76343715	None:intergenic	40.0%
AGGCAAAGTTACACTTACCT+TGG	-	Chr1:76343400-76343419	None:intergenic	40.0%
ATGGCATCATCATCATGTGT+AGG	+	Chr1:76343330-76343349	MsG0180004311.01.T01:CDS	40.0%
CAAAAGGGATGAGTTGTACT+TGG	+	Chr1:76344075-76344094	MsG0180004311.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	76343321	76344166	76343321	ID=MsG0180004311.01;Name=MsG0180004311.01
Chr1	mRNA	76343321	76344166	76343321	ID=MsG0180004311.01.T01;Parent=MsG0180004311.01;Name=MsG0180004311.01.T01;_AED=0.49;_eAED=0.49;_QI=9\|1\|1\|1\|1\|1\|2\|27\|135
Chr1	exon	76343321	76343532	76343321	ID=MsG0180004311.01.T01:exon:14800;Parent=MsG0180004311.01.T01
Chr1	exon	76343935	76344166	76343935	ID=MsG0180004311.01.T01:exon:14801;Parent=MsG0180004311.01.T01
Chr1	five_prime_UTR	76343321	76343329	76343321	ID=MsG0180004311.01.T01:five_prime_utr;Parent=MsG0180004311.01.T01
Chr1	CDS	76343330	76343532	76343330	ID=MsG0180004311.01.T01:cds;Parent=MsG0180004311.01.T01
Chr1	CDS	76343935	76344139	76343935	ID=MsG0180004311.01.T01:cds;Parent=MsG0180004311.01.T01
Chr1	three_prime_UTR	76344140	76344166	76344140	ID=MsG0180004311.01.T01:three_prime_utr;Parent=MsG0180004311.01.T01

Gene Sequence

>MsG0180004311.01.T01

ATGGCATCATCATCATGTGTAGGAACTCTATTTTCCTCACAAACCAAACATTTGACTGCTACCAAGCCCAAGGTAAGTGTAACTTTGCCTTCAACAAGGAGAAGAGTTGTAAGAGCAGCTGTCACAGAGAAACTTGGCATCAAGATTGAGAGCAACCCTCCTGAGTCCAAGCTCACTCAACTTGGTGTTAGGCAGTGGCCCAAATGGGGTTGTCCTCCAAGTAATTTTCCATGGACATATGATGCAACAGAGACATGCTATCTTTTGGAAGGAAAAGTGAAGGTCACTCCTAGTGGGGCTAATGAGGCAGTTGAAATTTCTGCTGGTGATTTGGTTGTTTTCCCAAAAGGGATGAGTTGTACTTGGGATGTTTCTGTTGGTGTGGATAAGCACTATAAATTTGAATAA

Protein sequence

>MsG0180004311.01.T01

MASSSCVGTLFSSQTKHLTATKPKVSVTLPSTRRRVVRAAVTEKLGIKIESNPPESKLTQLGVRQWPKWGCPPSNFPWTYDATETCYLLEGKVKVTPSGANEAVEISAGDLVVFPKGMSCTWDVSVGVDKHYKFE*