Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004659.01.T01 | XP_003591220.1 | 97.727 | 308 | 7 | 0 | 1 | 308 | 34 | 341 | 0 | 615 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004659.01.T01 | Q9XGI7 | 64.63 | 311 | 97 | 2 | 8 | 307 | 9 | 317 | 1.63E-145 | 414 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004659.01.T01 | G7I9I0 | 97.727 | 308 | 7 | 0 | 1 | 308 | 34 | 341 | 0.0 | 615 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004659.01 | MsG0280006306.01 | 0.807998 | 3.844087e-50 | 2.172623e-47 |
| MsG0180004659.01 | MsG0380015849.01 | 0.820000 | 8.642940e-53 | 6.756281e-50 |
| MsG0180004659.01 | MsG0480022872.01 | 0.802605 | 5.181322e-49 | 2.548899e-46 |
| MsG0180004659.01 | MsG0880042987.01 | 0.801770 | 7.695980e-49 | 3.706242e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004659.01.T01 | MTR_1g084050 | 97.727 | 308 | 7 | 0 | 1 | 308 | 34 | 341 | 0.0 | 615 |
| MsG0180004659.01.T01 | MTR_7g112130 | 71.963 | 321 | 74 | 4 | 1 | 308 | 1 | 318 | 4.26e-164 | 459 |
| MsG0180004659.01.T01 | MTR_2g034240 | 64.103 | 273 | 96 | 2 | 1 | 271 | 1 | 273 | 1.92e-124 | 357 |
| MsG0180004659.01.T01 | MTR_2g070310 | 49.438 | 267 | 135 | 0 | 5 | 271 | 15 | 281 | 2.42e-93 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004659.01.T01 | AT1G09240 | 62.284 | 289 | 101 | 4 | 1 | 281 | 1 | 289 | 1.20e-128 | 369 |
| MsG0180004659.01.T01 | AT1G56430 | 63.838 | 271 | 95 | 3 | 4 | 271 | 5 | 275 | 2.69e-127 | 366 |
| MsG0180004659.01.T01 | AT5G04950 | 55.975 | 318 | 123 | 5 | 1 | 303 | 1 | 316 | 1.41e-122 | 354 |
| MsG0180004659.01.T01 | AT5G56080 | 60.949 | 274 | 104 | 3 | 1 | 271 | 1 | 274 | 1.72e-120 | 349 |
| MsG0180004659.01.T01 | AT4G26483 | 60.000 | 60 | 22 | 1 | 220 | 277 | 1 | 60 | 2.77e-15 | 70.5 |
Find 60 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAATAAAATTGCCTTTAT+TGG | 0.082184 | 1:+80882937 | MsG0180004659.01.T01:CDS |
| TTGAGGTAGTTTGAATAATA+AGG | 0.175075 | 1:-80882865 | None:intergenic |
| AGAATATGAAGTTGTTTATT+TGG | 0.203723 | 1:+80883149 | MsG0180004659.01.T01:CDS |
| AACATACGCTTTGATAATTC+AGG | 0.264334 | 1:-80883084 | None:intergenic |
| ACCACATAGCCTAATGAGTT+TGG | 0.315382 | 1:-80882761 | None:intergenic |
| TTGAGTAGTGACTTTCTAAA+TGG | 0.330810 | 1:-80882795 | None:intergenic |
| TGAGGTAGTTTGAATAATAA+GGG | 0.360223 | 1:-80882864 | None:intergenic |
| GAGTAATATGATTGAGGAAT+TGG | 0.382360 | 1:+80883464 | MsG0180004659.01.T01:CDS |
| AGGGACATTAGTGGAATGTT+TGG | 0.382437 | 1:-80882917 | None:intergenic |
| TCATCGGAACGACATTTGTT+TGG | 0.395433 | 1:-80883408 | None:intergenic |
| ATGGTTGATCAGTGGTTTGA+AGG | 0.398617 | 1:-80883439 | None:intergenic |
| CAATGATCTTCGAGGCTTTG+AGG | 0.404062 | 1:+80883308 | MsG0180004659.01.T01:CDS |
| AAAATTGCCTTTATTGGCTC+AGG | 0.404555 | 1:+80882943 | MsG0180004659.01.T01:CDS |
| TGAAACATCCAATATATCAT+TGG | 0.412368 | 1:-80883115 | None:intergenic |
| CAAGCAAGAAGCTTTGGAAT+TGG | 0.412940 | 1:-80883049 | None:intergenic |
| TGAAACCAAGCAAGAAGCTT+TGG | 0.415124 | 1:-80883055 | None:intergenic |
| GCTAAGTTTGGTAACATCAA+TGG | 0.417679 | 1:-80882716 | None:intergenic |
| AAGCTTTGGAATTGGCTAAA+GGG | 0.426256 | 1:-80883041 | None:intergenic |
| TGGTTAGGATATTGAATTCA+AGG | 0.430102 | 1:-80882897 | None:intergenic |
| CATTAGTGGAATGTTTGGTT+AGG | 0.432644 | 1:-80882912 | None:intergenic |
| CTCAAAGCCTCGAAGATCAT+TGG | 0.441896 | 1:-80883307 | None:intergenic |
| TGTTTATTTGGCAGCACTTG+TGG | 0.443671 | 1:+80883161 | MsG0180004659.01.T01:CDS |
| AAATTAGATCCAAACTCATT+AGG | 0.446761 | 1:+80882752 | MsG0180004659.01.T01:CDS |
| GTTTATTTGGCAGCACTTGT+GGG | 0.458446 | 1:+80883162 | MsG0180004659.01.T01:CDS |
| CGGGAGCACTTCTCATGCTC+AGG | 0.459497 | 1:+80883241 | MsG0180004659.01.T01:CDS |
| CTAAGTTTGGTAACATCAAT+GGG | 0.471982 | 1:-80882715 | None:intergenic |
| ACAAGTGAAGTAAGTGGAAG+TGG | 0.474698 | 1:-80882967 | None:intergenic |
| CAATTCCAAAGCTTCTTGCT+TGG | 0.481966 | 1:+80883050 | MsG0180004659.01.T01:CDS |
| GAAGCTTTGGAATTGGCTAA+AGG | 0.486375 | 1:-80883042 | None:intergenic |
| AGATCATTGGTATCAACAAC+CGG | 0.488411 | 1:-80883294 | None:intergenic |
| AAGTGGACCTGAGCCAATAA+AGG | 0.493489 | 1:-80882950 | None:intergenic |
| GTTGATACCAATGATCTTCG+AGG | 0.499486 | 1:+80883300 | MsG0180004659.01.T01:CDS |
| GAATAAAATCATTGAGCACT+TGG | 0.501973 | 1:+80883203 | MsG0180004659.01.T01:CDS |
| ATTATTATATGAAGCTATGA+TGG | 0.524264 | 1:-80882818 | None:intergenic |
| ATTCAAGGAGACTAAGTTTG+AGG | 0.524306 | 1:-80882882 | None:intergenic |
| CATGCATGTGAGAACAAGTT+TGG | 0.533798 | 1:-80882683 | None:intergenic |
| CCTCAATCATATTACTCCCA+TGG | 0.537816 | 1:-80883458 | None:intergenic |
| TTGTTTGGTATTAAAACCAT+CGG | 0.545141 | 1:-80883393 | None:intergenic |
| TCCAAACTCATTAGGCTATG+TGG | 0.546869 | 1:+80882760 | MsG0180004659.01.T01:CDS |
| GCTAAGACAAGTGAAGTAAG+TGG | 0.548518 | 1:-80882973 | None:intergenic |
| CCATGGGAGTAATATGATTG+AGG | 0.558526 | 1:+80883458 | MsG0180004659.01.T01:CDS |
| CTCATGCTCAGGAGTGCTCA+CGG | 0.560813 | 1:+80883252 | MsG0180004659.01.T01:CDS |
| CTGAGCATGAGAAGTGCTCC+CGG | 0.560966 | 1:-80883240 | None:intergenic |
| TGAGCACTTGGCTAAGTACA+TGG | 0.562129 | 1:+80883215 | MsG0180004659.01.T01:CDS |
| CTTGGCTAAGTACATGGCAC+CGG | 0.568991 | 1:+80883221 | MsG0180004659.01.T01:CDS |
| GAATTGATAACCTCGTCTGT+TGG | 0.576403 | 1:-80883348 | None:intergenic |
| AAAATATTGAGTTGATTGAG+TGG | 0.578772 | 1:-80882841 | None:intergenic |
| TTTGAAGGCTTGAATCTCAT+CGG | 0.581027 | 1:-80883424 | None:intergenic |
| TTGGTAACATCAATGGGACT+AGG | 0.586535 | 1:-80882709 | None:intergenic |
| TAGTGTCGACATGGTTGGCA+AGG | 0.595518 | 1:+80882567 | None:intergenic |
| TTACTCCCATGGTTGATCAG+TGG | 0.597719 | 1:-80883447 | None:intergenic |
| TTCAAACCACTGATCAACCA+TGG | 0.608081 | 1:+80883441 | MsG0180004659.01.T01:CDS |
| AGGCTATGTGGTGAAGCTGA+AGG | 0.608399 | 1:+80882772 | MsG0180004659.01.T01:CDS |
| CATAGCGCGCAAGTATCCGA+TGG | 0.624484 | 1:+80883377 | MsG0180004659.01.T01:CDS |
| CCTCATAGTGTCGACATGGT+TGG | 0.629181 | 1:+80882562 | None:intergenic |
| TTGGCTAAGTACATGGCACC+GGG | 0.642493 | 1:+80883222 | MsG0180004659.01.T01:CDS |
| TCAAACCACTGATCAACCAT+GGG | 0.645985 | 1:+80883442 | MsG0180004659.01.T01:CDS |
| GTAACATCAATGGGACTAGG+TGG | 0.681662 | 1:-80882706 | None:intergenic |
| TGTGTTTCATCCAACAGACG+AGG | 0.716463 | 1:+80883338 | MsG0180004659.01.T01:CDS |
| ACTTGTGGGAATGAGTGTTG+AGG | 0.717943 | 1:+80883176 | MsG0180004659.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATATGAAGTTGTTTATT+TGG | + | Chr1:80883149-80883168 | MsG0180004659.01.T01:CDS | 20.0% |
| !! | ATTATTATATGAAGCTATGA+TGG | - | Chr1:80882821-80882840 | None:intergenic | 20.0% |
| !!! | CAATAAAGGCAATTTTATTA+GGG | - | Chr1:80882939-80882958 | None:intergenic | 20.0% |
| !!! | TTTTTACACCAATGATATAT+TGG | + | Chr1:80883107-80883126 | MsG0180004659.01.T01:CDS | 20.0% |
| ! | AAATTAGATCCAAACTCATT+AGG | + | Chr1:80882752-80882771 | MsG0180004659.01.T01:CDS | 25.0% |
| ! | CCTAATAAAATTGCCTTTAT+TGG | + | Chr1:80882937-80882956 | MsG0180004659.01.T01:CDS | 25.0% |
| ! | TGAAACATCCAATATATCAT+TGG | - | Chr1:80883118-80883137 | None:intergenic | 25.0% |
| ! | TGAGGTAGTTTGAATAATAA+GGG | - | Chr1:80882867-80882886 | None:intergenic | 25.0% |
| ! | TTGAGGTAGTTTGAATAATA+AGG | - | Chr1:80882868-80882887 | None:intergenic | 25.0% |
| ! | TTGTTTGGTATTAAAACCAT+CGG | - | Chr1:80883396-80883415 | None:intergenic | 25.0% |
| !! | AATTTTATTAGGGACATTAG+TGG | - | Chr1:80882929-80882948 | None:intergenic | 25.0% |
| !! | CCAATAAAGGCAATTTTATT+AGG | - | Chr1:80882940-80882959 | None:intergenic | 25.0% |
| !! | TGTGAAAAATTGTGTTTTGT+AGG | - | Chr1:80883008-80883027 | None:intergenic | 25.0% |
| !!! | AAAATATTGAGTTGATTGAG+TGG | - | Chr1:80882844-80882863 | None:intergenic | 25.0% |
| GAATAAAATCATTGAGCACT+TGG | + | Chr1:80883203-80883222 | MsG0180004659.01.T01:CDS | 30.0% | |
| GAGTAATATGATTGAGGAAT+TGG | + | Chr1:80883464-80883483 | MsG0180004659.01.T01:CDS | 30.0% | |
| TGGTTAGGATATTGAATTCA+AGG | - | Chr1:80882900-80882919 | None:intergenic | 30.0% | |
| TTGAGTAGTGACTTTCTAAA+TGG | - | Chr1:80882798-80882817 | None:intergenic | 30.0% | |
| ! | AACATACGCTTTGATAATTC+AGG | - | Chr1:80883087-80883106 | None:intergenic | 30.0% |
| ! | CTAAGTTTGGTAACATCAAT+GGG | - | Chr1:80882718-80882737 | None:intergenic | 30.0% |
| ! | TTGAACTTTTGTGCTAAGTT+TGG | - | Chr1:80882731-80882750 | None:intergenic | 30.0% |
| AGATCATTGGTATCAACAAC+CGG | - | Chr1:80883297-80883316 | None:intergenic | 35.0% | |
| ATTCAAGGAGACTAAGTTTG+AGG | - | Chr1:80882885-80882904 | None:intergenic | 35.0% | |
| CATTAGTGGAATGTTTGGTT+AGG | - | Chr1:80882915-80882934 | None:intergenic | 35.0% | |
| TTTGAAGGCTTGAATCTCAT+CGG | - | Chr1:80883427-80883446 | None:intergenic | 35.0% | |
| ! | GCTAAGTTTGGTAACATCAA+TGG | - | Chr1:80882719-80882738 | None:intergenic | 35.0% |
| ! | TGTGTTTTGTAGGTGATTAG+TGG | - | Chr1:80882998-80883017 | None:intergenic | 35.0% |
| !! | AAAATTGCCTTTATTGGCTC+AGG | + | Chr1:80882943-80882962 | MsG0180004659.01.T01:CDS | 35.0% |
| !! | AAGCTTTGGAATTGGCTAAA+GGG | - | Chr1:80883044-80883063 | None:intergenic | 35.0% |
| !! | AGTGTGTTGACATTTTTGCA+AGG | - | Chr1:80882658-80882677 | None:intergenic | 35.0% |
| ACAAGTGAAGTAAGTGGAAG+TGG | - | Chr1:80882970-80882989 | None:intergenic | 40.0% | |
| ACCACATAGCCTAATGAGTT+TGG | - | Chr1:80882764-80882783 | None:intergenic | 40.0% | |
| AGGGACATTAGTGGAATGTT+TGG | - | Chr1:80882920-80882939 | None:intergenic | 40.0% | |
| ATGGTTGATCAGTGGTTTGA+AGG | - | Chr1:80883442-80883461 | None:intergenic | 40.0% | |
| CAATTCCAAAGCTTCTTGCT+TGG | + | Chr1:80883050-80883069 | MsG0180004659.01.T01:CDS | 40.0% | |
| CATGCATGTGAGAACAAGTT+TGG | - | Chr1:80882686-80882705 | None:intergenic | 40.0% | |
| CCATGGGAGTAATATGATTG+AGG | + | Chr1:80883458-80883477 | MsG0180004659.01.T01:CDS | 40.0% | |
| CCTCAATCATATTACTCCCA+TGG | - | Chr1:80883461-80883480 | None:intergenic | 40.0% | |
| GCTAAGACAAGTGAAGTAAG+TGG | - | Chr1:80882976-80882995 | None:intergenic | 40.0% | |
| GTTGATACCAATGATCTTCG+AGG | + | Chr1:80883300-80883319 | MsG0180004659.01.T01:CDS | 40.0% | |
| GTTTATTTGGCAGCACTTGT+GGG | + | Chr1:80883162-80883181 | MsG0180004659.01.T01:CDS | 40.0% | |
| TCAAACCACTGATCAACCAT+GGG | + | Chr1:80883442-80883461 | MsG0180004659.01.T01:CDS | 40.0% | |
| TCATCGGAACGACATTTGTT+TGG | - | Chr1:80883411-80883430 | None:intergenic | 40.0% | |
| TCCAAACTCATTAGGCTATG+TGG | + | Chr1:80882760-80882779 | MsG0180004659.01.T01:CDS | 40.0% | |
| TGAAACCAAGCAAGAAGCTT+TGG | - | Chr1:80883058-80883077 | None:intergenic | 40.0% | |
| TGTTTATTTGGCAGCACTTG+TGG | + | Chr1:80883161-80883180 | MsG0180004659.01.T01:CDS | 40.0% | |
| TTCAAACCACTGATCAACCA+TGG | + | Chr1:80883441-80883460 | MsG0180004659.01.T01:CDS | 40.0% | |
| ! | GAATTGATAACCTCGTCTGT+TGG | - | Chr1:80883351-80883370 | None:intergenic | 40.0% |
| ! | TTGGTAACATCAATGGGACT+AGG | - | Chr1:80882712-80882731 | None:intergenic | 40.0% |
| !! | CAAGCAAGAAGCTTTGGAAT+TGG | - | Chr1:80883052-80883071 | None:intergenic | 40.0% |
| !! | GAAGCTTTGGAATTGGCTAA+AGG | - | Chr1:80883045-80883064 | None:intergenic | 40.0% |
| AAGTGGACCTGAGCCAATAA+AGG | - | Chr1:80882953-80882972 | None:intergenic | 45.0% | |
| CAATGATCTTCGAGGCTTTG+AGG | + | Chr1:80883308-80883327 | MsG0180004659.01.T01:CDS | 45.0% | |
| CTCAAAGCCTCGAAGATCAT+TGG | - | Chr1:80883310-80883329 | None:intergenic | 45.0% | |
| GTAACATCAATGGGACTAGG+TGG | - | Chr1:80882709-80882728 | None:intergenic | 45.0% | |
| TGAGCACTTGGCTAAGTACA+TGG | + | Chr1:80883215-80883234 | MsG0180004659.01.T01:CDS | 45.0% | |
| TGTGTTTCATCCAACAGACG+AGG | + | Chr1:80883338-80883357 | MsG0180004659.01.T01:CDS | 45.0% | |
| TTACTCCCATGGTTGATCAG+TGG | - | Chr1:80883450-80883469 | None:intergenic | 45.0% | |
| ! | ACTTGTGGGAATGAGTGTTG+AGG | + | Chr1:80883176-80883195 | MsG0180004659.01.T01:CDS | 45.0% |
| ! | TGCTCGTGCTTTTCTGTATC+CGG | + | Chr1:80883275-80883294 | MsG0180004659.01.T01:CDS | 45.0% |
| AGGCTATGTGGTGAAGCTGA+AGG | + | Chr1:80882772-80882791 | MsG0180004659.01.T01:CDS | 50.0% | |
| CTTGGCTAAGTACATGGCAC+CGG | + | Chr1:80883221-80883240 | MsG0180004659.01.T01:CDS | 50.0% | |
| TTGGCTAAGTACATGGCACC+GGG | + | Chr1:80883222-80883241 | MsG0180004659.01.T01:CDS | 50.0% | |
| CATAGCGCGCAAGTATCCGA+TGG | + | Chr1:80883377-80883396 | MsG0180004659.01.T01:CDS | 55.0% | |
| !! | CTCATGCTCAGGAGTGCTCA+CGG | + | Chr1:80883252-80883271 | MsG0180004659.01.T01:CDS | 55.0% |
| !! | CTGAGCATGAGAAGTGCTCC+CGG | - | Chr1:80883243-80883262 | None:intergenic | 55.0% |
| !! | CGGGAGCACTTCTCATGCTC+AGG | + | Chr1:80883241-80883260 | MsG0180004659.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 80882577 | 80883503 | 80882577 | ID=MsG0180004659.01;Name=MsG0180004659.01 |
| Chr1 | mRNA | 80882577 | 80883503 | 80882577 | ID=MsG0180004659.01.T01;Parent=MsG0180004659.01;Name=MsG0180004659.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|308 |
| Chr1 | exon | 80882577 | 80883503 | 80882577 | ID=MsG0180004659.01.T01:exon:12035;Parent=MsG0180004659.01.T01 |
| Chr1 | CDS | 80882577 | 80883503 | 80882577 | ID=MsG0180004659.01.T01:cds;Parent=MsG0180004659.01.T01 |
| Gene Sequence |
| Protein sequence |
