AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015849.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015849.01.T01 MTR_3g078610 76.636 107 8 1 1 107 1 90 7.96e-39 129
MsG0380015849.01.T01 MTR_3g078610 76.636 107 8 1 1 107 1 90 8.37e-39 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAGGAGGTTGTTGAGAT+TGG 0.306031 3:-75830173 None:intergenic
CGGAGATGACGGTGGGGAAG+GGG 0.343868 3:-75830116 None:intergenic
CGGTGGGGAAGGGGAGGAAG+AGG 0.363507 3:-75830107 None:intergenic
CCAATTCTTCGCATCCTCAT+TGG 0.380648 3:+75830047 MsG0380015849.01.T01:CDS
CCAATGAGGATGCGAAGAAT+TGG 0.384540 3:-75830047 None:intergenic
TGGGGAAGGGGAGGAAGAGG+TGG 0.403774 3:-75830104 None:intergenic
GTTGAGATTGGAGGCGGTAG+TGG 0.410912 3:-75830161 None:intergenic
GGCGGTAGTGGTGGCGTTTG+CGG 0.421186 3:-75830149 None:intergenic
AGTGGTGGCGTTTGCGGCGG+CGG 0.425892 3:-75830143 None:intergenic
TCGGAGATGACGGTGGGGAA+GGG 0.440083 3:-75830117 None:intergenic
AATTCATTTCTTCAATTGCA+TGG 0.442372 3:+75830255 MsG0380015849.01.T01:CDS
AGATGACGGTGGGGAAGGGG+AGG 0.442459 3:-75830113 None:intergenic
ATCGGAGATGACGGTGGGGA+AGG 0.452043 3:-75830118 None:intergenic
GCGTTTGCGGCGGCGGAGAT+CGG 0.463231 3:-75830136 None:intergenic
CGGAGATCGGAGATGACGGT+GGG 0.504404 3:-75830123 None:intergenic
GAGGTTGTTGAGATTGGAGG+CGG 0.509019 3:-75830167 None:intergenic
ACCCGCGGATTCACTTTCGC+TGG 0.514723 3:+75830217 MsG0380015849.01.T01:CDS
GAGGAGGTTGTTGAGATTGG+AGG 0.537669 3:-75830170 None:intergenic
GGTAGTGGTGGCGTTTGCGG+CGG 0.570718 3:-75830146 None:intergenic
GGAAATTTGATTGAAAATCG+AGG 0.584509 3:+75830366 MsG0380015849.01.T01:CDS
ACAACCTCCTCACTGCTCCA+TGG 0.587232 3:+75830182 MsG0380015849.01.T01:CDS
CGGGTTTGAGAAGCGGACCA+TGG 0.595025 3:-75830199 None:intergenic
GCGGCGGAGATCGGAGATGA+CGG 0.602075 3:-75830127 None:intergenic
GCGGAGATCGGAGATGACGG+TGG 0.606744 3:-75830124 None:intergenic
ATCCAGCGAAAGTGAATCCG+CGG 0.606920 3:-75830219 None:intergenic
TATCACTGAAACTCAGCAAG+AGG 0.610373 3:+75830020 MsG0380015849.01.T01:CDS
GAATCCGCGGGTTTGAGAAG+CGG 0.621886 3:-75830206 None:intergenic
GAGATTGGAGGCGGTAGTGG+TGG 0.631668 3:-75830158 None:intergenic
TCCAGCGAAAGTGAATCCGC+GGG 0.636866 3:-75830218 None:intergenic
GGAGATCGGAGATGACGGTG+GGG 0.638493 3:-75830122 None:intergenic
TTTGATGAGATTCTCCAATG+AGG 0.640483 3:-75830061 None:intergenic
CGGACCATGGAGCAGTGAGG+AGG 0.665229 3:-75830186 None:intergenic
TGGTCCGCTTCTCAAACCCG+CGG 0.679979 3:+75830202 MsG0380015849.01.T01:CDS
AAGCGGACCATGGAGCAGTG+AGG 0.702686 3:-75830189 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTCATTTCTTCAATTGCA+TGG + Chr3:75830255-75830274 MsG0380015849.01.T01:CDS 25.0%
GGAAATTTGATTGAAAATCG+AGG + Chr3:75830366-75830385 MsG0380015849.01.T01:CDS 30.0%
TTTGATGAGATTCTCCAATG+AGG - Chr3:75830064-75830083 None:intergenic 35.0%
!!! ACTGTTTCTACTTGTTTTGC+AGG + Chr3:75830335-75830354 MsG0380015849.01.T01:intron 35.0%
TATCACTGAAACTCAGCAAG+AGG + Chr3:75830020-75830039 MsG0380015849.01.T01:CDS 40.0%
!!! CTTGTTTTGCAGGTTAGAGT+TGG + Chr3:75830345-75830364 MsG0380015849.01.T01:intron 40.0%
AGTGAGGAGGTTGTTGAGAT+TGG - Chr3:75830176-75830195 None:intergenic 45.0%
CCAATGAGGATGCGAAGAAT+TGG - Chr3:75830050-75830069 None:intergenic 45.0%
CCAATTCTTCGCATCCTCAT+TGG + Chr3:75830047-75830066 MsG0380015849.01.T01:CDS 45.0%
ATCCAGCGAAAGTGAATCCG+CGG - Chr3:75830222-75830241 None:intergenic 50.0%
GAGGAGGTTGTTGAGATTGG+AGG - Chr3:75830173-75830192 None:intergenic 50.0%
GAGGTTGTTGAGATTGGAGG+CGG - Chr3:75830170-75830189 None:intergenic 50.0%
ACAACCTCCTCACTGCTCCA+TGG + Chr3:75830182-75830201 MsG0380015849.01.T01:CDS 55.0%
GAATCCGCGGGTTTGAGAAG+CGG - Chr3:75830209-75830228 None:intergenic 55.0%
GTTGAGATTGGAGGCGGTAG+TGG - Chr3:75830164-75830183 None:intergenic 55.0%
TCCAGCGAAAGTGAATCCGC+GGG - Chr3:75830221-75830240 None:intergenic 55.0%
AAGCGGACCATGGAGCAGTG+AGG - Chr3:75830192-75830211 None:intergenic 60.0%
ACCCGCGGATTCACTTTCGC+TGG + Chr3:75830217-75830236 MsG0380015849.01.T01:CDS 60.0%
CGGGTTTGAGAAGCGGACCA+TGG - Chr3:75830202-75830221 None:intergenic 60.0%
TGGTCCGCTTCTCAAACCCG+CGG + Chr3:75830202-75830221 MsG0380015849.01.T01:CDS 60.0%
! ATCGGAGATGACGGTGGGGA+AGG - Chr3:75830121-75830140 None:intergenic 60.0%
! CGGAGATCGGAGATGACGGT+GGG - Chr3:75830126-75830145 None:intergenic 60.0%
! GAGATTGGAGGCGGTAGTGG+TGG - Chr3:75830161-75830180 None:intergenic 60.0%
! GGAGATCGGAGATGACGGTG+GGG - Chr3:75830125-75830144 None:intergenic 60.0%
! TCGGAGATGACGGTGGGGAA+GGG - Chr3:75830120-75830139 None:intergenic 60.0%
CGGACCATGGAGCAGTGAGG+AGG - Chr3:75830189-75830208 None:intergenic 65.0%
GCGGCGGAGATCGGAGATGA+CGG - Chr3:75830130-75830149 None:intergenic 65.0%
TGGGGAAGGGGAGGAAGAGG+TGG - Chr3:75830107-75830126 None:intergenic 65.0%
! AGATGACGGTGGGGAAGGGG+AGG - Chr3:75830116-75830135 None:intergenic 65.0%
! CGGAGATGACGGTGGGGAAG+GGG - Chr3:75830119-75830138 None:intergenic 65.0%
! GCGGAGATCGGAGATGACGG+TGG - Chr3:75830127-75830146 None:intergenic 65.0%
! GGCGGTAGTGGTGGCGTTTG+CGG - Chr3:75830152-75830171 None:intergenic 65.0%
! GGTAGTGGTGGCGTTTGCGG+CGG - Chr3:75830149-75830168 None:intergenic 65.0%
CGGTGGGGAAGGGGAGGAAG+AGG - Chr3:75830110-75830129 None:intergenic 70.0%
GCGTTTGCGGCGGCGGAGAT+CGG - Chr3:75830139-75830158 None:intergenic 70.0%
! AGTGGTGGCGTTTGCGGCGG+CGG - Chr3:75830146-75830165 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 75830010 75830404 75830010 ID=MsG0380015849.01;Name=MsG0380015849.01
Chr3 mRNA 75830010 75830404 75830010 ID=MsG0380015849.01.T01;Parent=MsG0380015849.01;Name=MsG0380015849.01.T01;_AED=0.50;_eAED=0.58;_QI=0|0|0|1|0|0|2|0|111
Chr3 exon 75830010 75830297 75830010 ID=MsG0380015849.01.T01:exon:24854;Parent=MsG0380015849.01.T01
Chr3 exon 75830357 75830404 75830357 ID=MsG0380015849.01.T01:exon:24855;Parent=MsG0380015849.01.T01
Chr3 CDS 75830010 75830297 75830010 ID=MsG0380015849.01.T01:cds;Parent=MsG0380015849.01.T01
Chr3 CDS 75830357 75830404 75830357 ID=MsG0380015849.01.T01:cds;Parent=MsG0380015849.01.T01
Gene Sequence

>MsG0380015849.01.T01

ATGCAATCTCTATCACTGAAACTCAGCAAGAGGCTCTCCAATTCTTCGCATCCTCATTGGAGAATCTCATCAAACTTCATCGCTCACGCTTCAACCACCTCTTCCTCCCCTTCCCCACCGTCATCTCCGATCTCCGCCGCCGCAAACGCCACCACTACCGCCTCCAATCTCAACAACCTCCTCACTGCTCCATGGTCCGCTTCTCAAACCCGCGGATTCACTTTCGCTGGATCTGATGTAAGCGTAATTCATTTCTTCAATTGCATGGATCTTTTCAATTTTGAAATCGTTAGAGTTGGAAATTTGATTGAAAATCGAGGTAGTTTTGATTTTTAA

Protein sequence

>MsG0380015849.01.T01

MQSLSLKLSKRLSNSSHPHWRISSNFIAHASTTSSSPSPPSSPISAAANATTTASNLNNLLTAPWSASQTRGFTFAGSDVSVIHFFNCMDLFNFEIVRVGNLIENRGSFDF*