AlfalfaGEDB | MsG0180004859.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004859.01

KEGG: K08678
GO: -
PFAM: PF16363

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004859.01.T01	XP_024639560.1	89.195	435	15	3	1	403	1	435	0	756

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004859.01.T01	Q9LZI2	69.3	443	81	7	1	399	1	432	0	601

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004859.01.T01	A0A396JV24	87.140	451	10	2	1	403	1	451	0.0	785

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048212.01	MsG0180004859.01	0.923712	1.848992e-89	7.515221e-85
MsG0080048917.01	MsG0180004859.01	-0.820786	5.706547e-53	4.559038e-50
MsG0080048918.01	MsG0180004859.01	-0.806027	1.003903e-49	5.392202e-47
MsG0080048919.01	MsG0180004859.01	-0.814066	1.861163e-51	1.236323e-48
MsG0080048978.01	MsG0180004859.01	-0.813661	2.286417e-51	1.502189e-48
MsG0080049053.01	MsG0180004859.01	-0.810187	1.305713e-50	7.818462e-48
MsG0080049062.01	MsG0180004859.01	-0.801686	8.008384e-49	3.848454e-46
MsG0080049066.01	MsG0180004859.01	-0.866873	2.073563e-65	7.097619e-62
MsG0080049133.01	MsG0180004859.01	-0.830237	3.289232e-55	3.445544e-52
MsG0180000193.01	MsG0180004859.01	-0.802559	5.297710e-49	2.602962e-46

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PPI

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Gene1	Gene2	Type
MsG0080048977.01	MsG0180004859.01	PPI
MsG0180000280.01	MsG0180004859.01	PPI
MsG0180004859.01	MsG0180003694.01	PPI
MsG0180004859.01	MsG0780038271.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004859.01.T01	MTR_1g088480	88.837	430	21	1	1	403	1	430	0.0	751
MsG0180004859.01.T01	MTR_4g120030	60.412	437	125	7	1	403	1	423	5.30e-179	505
MsG0180004859.01.T01	MTR_1g067530	50.441	454	133	9	19	400	25	458	1.23e-131	386
MsG0180004859.01.T01	MTR_4g063600	59.306	317	96	3	115	398	29	345	5.53e-127	370
MsG0180004859.01.T01	MTR_4g063600	59.554	314	97	4	115	398	29	342	1.27e-125	366
MsG0180004859.01.T01	MTR_2g096660	59.486	311	93	3	115	392	35	345	1.59e-124	364
MsG0180004859.01.T01	MTR_7g115080	26.380	326	194	9	118	402	63	383	2.42e-22	98.6
MsG0180004859.01.T01	MTR_7g115080	26.380	326	194	9	118	402	29	349	3.19e-22	97.8
MsG0180004859.01.T01	MTR_7g115080	26.380	326	194	9	118	402	42	362	3.80e-22	97.4
MsG0180004859.01.T01	MTR_1g080950	25.617	324	195	8	118	399	28	347	7.10e-22	96.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004859.01.T01	AT3G62830	69.300	443	81	7	1	399	1	432	0.0	601
MsG0180004859.01.T01	AT3G62830	69.300	443	81	7	1	399	1	432	0.0	601
MsG0180004859.01.T01	AT2G47650	69.635	438	88	7	1	398	1	433	0.0	593
MsG0180004859.01.T01	AT2G47650	68.694	444	88	8	1	398	1	439	0.0	587
MsG0180004859.01.T01	AT3G53520	62.830	417	113	6	19	400	25	434	2.28e-179	506
MsG0180004859.01.T01	AT3G53520	62.350	417	113	7	19	400	25	432	4.04e-176	498
MsG0180004859.01.T01	AT3G53520	59.091	440	115	8	19	400	25	457	1.28e-170	485
MsG0180004859.01.T01	AT2G28760	60.772	311	89	3	115	392	28	338	1.10e-129	377
MsG0180004859.01.T01	AT2G28760	60.772	311	89	3	115	392	28	338	1.10e-129	377
MsG0180004859.01.T01	AT2G28760	60.772	311	89	3	115	392	28	338	1.10e-129	377

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Find 142 sgRNAs with CRISPR-Local

Find 349 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGAGCCTTTGACAGTTTA+TGG	0.132583	1:-83680792	MsG0180004859.01.T01:CDS
AATCAATCTTCAAAGATTGA+GGG	0.543476	1:+83678718	None:intergenic
AATGAAATCATGGCTGGCTA+TGG	0.506217	1:+83678690	None:intergenic
ACAGAGAGCCATGGTTTGTT+TGG	0.370816	1:+83683037	None:intergenic
ACATACCACCATCAAGAATT+AGG	0.334411	1:+83678875	None:intergenic
ACCCAAGGTGGACCTGCGCA+AGG	0.544301	1:-83678967	MsG0180004859.01.T01:CDS
ACTGGTGGAGCGGGGTTCGT+TGG	0.383783	1:-83682737	MsG0180004859.01.T01:CDS
AGATAAAGAAATAATGATAA+AGG	0.379083	1:+83682484	None:intergenic
AGATTCCGTTAGGGATAAAG+AGG	0.565904	1:-83682781	MsG0180004859.01.T01:CDS
AGCCATGGTTTGTTTGGTTT+TGG	0.308012	1:+83683043	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTACCATCTATGATGCA+CGG	+	Chr1:83679994-83680013	None:intergenic	30.0%
AATAAATTGTTGTGTGAGAC+AGG	-	Chr1:83680328-83680347	MsG0180004859.01.T01:intron	30.0%
AATTTCAAACAACAAAGCCA+CGG	-	Chr1:83680712-83680731	MsG0180004859.01.T01:intron	30.0%
ACTCTATTCTGGACTATATT+TGG	-	Chr1:83680913-83680932	MsG0180004859.01.T01:intron	30.0%
AGGTATGATTGTGAAGAATA+CGG	+	Chr1:83678654-83678673	None:intergenic	30.0%
AGTACTCCAAAATTCAAACA+TGG	+	Chr1:83681567-83681586	None:intergenic	30.0%
AGTTGGAGCAAAATAGAATA+AGG	+	Chr1:83683029-83683048	None:intergenic	30.0%
ATTTAATTATTCACGGTCAC+GGG	+	Chr1:83681918-83681937	None:intergenic	30.0%
CAAATGATTATGAGCTATTC+TGG	+	Chr1:83682154-83682173	None:intergenic	30.0%
CAAGGTAAAGATTATTAGGA+AGG	-	Chr1:83682125-83682144	MsG0180004859.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	83678491	83683125	83678491	ID=MsG0180004859.01;Name=MsG0180004859.01
Chr1	mRNA	83678491	83683125	83678491	ID=MsG0180004859.01.T01;Parent=MsG0180004859.01;Name=MsG0180004859.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0.8\|0.83\|1\|1\|1\|6\|402\|403
Chr1	exon	83678491	83679105	83678491	ID=MsG0180004859.01.T01:exon:9046;Parent=MsG0180004859.01.T01
Chr1	exon	83679488	83679580	83679488	ID=MsG0180004859.01.T01:exon:9045;Parent=MsG0180004859.01.T01
Chr1	exon	83680749	83680823	83680749	ID=MsG0180004859.01.T01:exon:9044;Parent=MsG0180004859.01.T01
Chr1	exon	83680937	83681020	83680937	ID=MsG0180004859.01.T01:exon:9043;Parent=MsG0180004859.01.T01
Chr1	exon	83681187	83681266	83681187	ID=MsG0180004859.01.T01:exon:9042;Parent=MsG0180004859.01.T01
Chr1	exon	83682459	83683125	83682459	ID=MsG0180004859.01.T01:exon:9041;Parent=MsG0180004859.01.T01
Chr1	CDS	83682459	83683125	83682459	ID=MsG0180004859.01.T01:cds;Parent=MsG0180004859.01.T01
Chr1	CDS	83681187	83681266	83681187	ID=MsG0180004859.01.T01:cds;Parent=MsG0180004859.01.T01
Chr1	CDS	83680937	83681020	83680937	ID=MsG0180004859.01.T01:cds;Parent=MsG0180004859.01.T01
Chr1	CDS	83680749	83680823	83680749	ID=MsG0180004859.01.T01:cds;Parent=MsG0180004859.01.T01
Chr1	CDS	83679488	83679580	83679488	ID=MsG0180004859.01.T01:cds;Parent=MsG0180004859.01.T01
Chr1	CDS	83678893	83679105	83678893	ID=MsG0180004859.01.T01:cds;Parent=MsG0180004859.01.T01
Chr1	three_prime_UTR	83678491	83678892	83678491	ID=MsG0180004859.01.T01:three_prime_utr;Parent=MsG0180004859.01.T01

Gene Sequence

>MsG0180004859.01.T01

ATGAGTTCAGAATTGACTTTCAGAGGACACGAAACTCAGCAAGTCAACGATGAATACTCACCAAAACCAAACAAACCATGGCTCTCTGTAATTCGTCCGATTCGTTACATGCTTCGTGAACAACGACTCGTTTTCGTCTTGATCGGAATCGTCATCGCATCCGTATTCTTCACAATCATACCTTCATCTTCAACTTCTTCATCTTCATCATTCTCAACCCGTCCATACGAATCAGACTCGATCTCTTACTTCGACCGCGAGTCAAAGACCACACCGGCAGTGTATAAGCAACGCGCTGCCGCGGTGGTTCATTCGTCCGGGAAGATTCCGTTAGGGATAAAGAGGAAGGGGCTGCGGATCGTGGTGACTGGTGGAGCGGGGTTCGTTGGGTCCCATCTAGTGGATCGGTTGATGGCGAGAGGGGACAGTGTGATTGTTGTTGATAATTTCTTCACTGGTAGAAAGGAGAACGTTATGCATCATTTTGGTAATCCGAGATTTGAGCTTATTCGACACGACGTCGTTGAGCCTCTTCTTCTTGAAGTTGATCAGATCTATCATCTTGCTTGTCCTGCTTCTCCTGTTCATTACAAGTTCAACCCCGTCAAAACTATCATATCCTTTATCATTATTTCTTTATCTGATCAAAGATGTGTCTGGTATCTGTGTGTCCGAAGCTGCTACGATGAGGGAAAGCGTACGGCTGAGACATTGACTATGGATTATCACAGAGGGGCCGGTGTAGAGGTTAGAATTGCTAGGATCTTTAATACATATGGACCCAGAATGTGCTTAGATGATGGACGTGTCGTTAGTAACTTCGTTGCTCAGGCGCTGAGGAAGGAGCCTTTGACAGTTTATGGGGATGGGAAGCAGACTAGAAGTTTCCAATACGTCTCTGACTTGGTGGAGGGTCTAATGCGCCTTATGGAAGGGGAACATGTGGGGCCGTTCAACCTTGGAAATCCTGGTGAATTTACCATGCTTGAACTTGCCAAGGTGGTCCAAGAAACAATCGACCCAGATGCAAAGATAGTGTACAGAGATAATACAGAGGATGACCCACACAAAAGAAAGCCTGATATTAGTAATGCTAAGGAGCACCTTGGCTGGGAACCCAAGGTGGACCTGCGCAAGGGCCTCCCACTAATGGTTTCCGACTTCCGACAACGCATTTTTGGTGACCATAAGGAAGGTGGATCTGATGCCTAA

Protein sequence

>MsG0180004859.01.T01

MSSELTFRGHETQQVNDEYSPKPNKPWLSVIRPIRYMLREQRLVFVLIGIVIASVFFTIIPSSSTSSSSSFSTRPYESDSISYFDRESKTTPAVYKQRAAAVVHSSGKIPLGIKRKGLRIVVTGGAGFVGSHLVDRLMARGDSVIVVDNFFTGRKENVMHHFGNPRFELIRHDVVEPLLLEVDQIYHLACPASPVHYKFNPVKTIISFIIISLSDQRCVWYLCVRSCYDEGKRTAETLTMDYHRGAGVEVRIARIFNTYGPRMCLDDGRVVSNFVAQALRKEPLTVYGDGKQTRSFQYVSDLVEGLMRLMEGEHVGPFNLGNPGEFTMLELAKVVQETIDPDAKIVYRDNTEDDPHKRKPDISNAKEHLGWEPKVDLRKGLPLMVSDFRQRIFGDHKEGGSDA*