Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005045.01.T01 | GAU12760.1 | 39.024 | 205 | 121 | 2 | 1 | 201 | 1 | 205 | 7.71E-34 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048221.01 | MsG0180005045.01 | 0.974439 | 3.699784e-138 | 5.122637e-132 |
| MsG0080048224.01 | MsG0180005045.01 | 0.981647 | 4.215371e-153 | 1.119424e-146 |
| MsG0180005045.01 | MsG0380016182.01 | 0.803818 | 2.906848e-49 | 1.475132e-46 |
| MsG0180005045.01 | MsG0480022933.01 | 0.806450 | 8.177805e-50 | 4.440092e-47 |
| MsG0180005045.01 | MsG0780036117.01 | 0.800946 | 1.135122e-48 | 5.354238e-46 |
| MsG0180005045.01 | MsG0880042743.01 | 0.815558 | 8.691141e-52 | 6.011954e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAATTCAAGGAGTTTC+TGG | 0.123756 | 1:-86157957 | None:intergenic |
| TATTGATTACTTGTCCTTTC+AGG | 0.165047 | 1:+86157613 | MsG0180005045.01.T01:CDS |
| GTCGAAAAGATCTAAAATTT+TGG | 0.178634 | 1:+86157382 | MsG0180005045.01.T01:CDS |
| TCCTTTGCCTTTCGAATTCA+TGG | 0.301017 | 1:+86157442 | MsG0180005045.01.T01:CDS |
| GCTGTTGTATTGTAGATTAT+TGG | 0.305986 | 1:-86157711 | None:intergenic |
| GACAATACGAAAGCTCAAAT+TGG | 0.319470 | 1:+86157643 | MsG0180005045.01.T01:CDS |
| AAGGAGGAGGAAGAGTTATT+TGG | 0.323852 | 1:-86157514 | None:intergenic |
| CCTCAAATGGTTCGAGAAAC+AGG | 0.344909 | 1:+86157874 | MsG0180005045.01.T01:CDS |
| TAATCAATAATTTCCAAGAT+TGG | 0.354103 | 1:-86157599 | None:intergenic |
| AAGCTCAAATTGGCTAAGTT+TGG | 0.359836 | 1:+86157653 | MsG0180005045.01.T01:CDS |
| ATTTGAGCTTTCGTATTGTC+AGG | 0.369492 | 1:-86157640 | None:intergenic |
| TCTTCGCAGACTTCCAATCT+TGG | 0.389719 | 1:+86157586 | MsG0180005045.01.T01:CDS |
| TGGCTAAGTTTGGTGGATCT+TGG | 0.390395 | 1:+86157663 | MsG0180005045.01.T01:CDS |
| CCTGTTTCTCGAACCATTTG+AGG | 0.415023 | 1:-86157874 | None:intergenic |
| CGCCATCTCGCATAAGGTGC+TGG | 0.424742 | 1:-86157415 | None:intergenic |
| CTCAAATTGGCTAAGTTTGG+TGG | 0.431809 | 1:+86157656 | MsG0180005045.01.T01:CDS |
| GGTGATCGGTTGGTGATATT+CGG | 0.443933 | 1:+86157794 | MsG0180005045.01.T01:CDS |
| GAGGCGGTAACGCAGGATCT+TGG | 0.445121 | 1:-86157855 | None:intergenic |
| GACCTTCGCCATCTCGCATA+AGG | 0.463108 | 1:-86157421 | None:intergenic |
| TCTTGGCACGCTCACTAGTA+AGG | 0.467933 | 1:-86157838 | None:intergenic |
| TTGCAGCTATTATTGAAAAG+AGG | 0.472869 | 1:+86157918 | MsG0180005045.01.T01:CDS |
| TTCAATAATAGCTGCAAGCT+CGG | 0.476416 | 1:-86157912 | None:intergenic |
| CAGCGAACACCTTCTCCAGC+TGG | 0.484890 | 1:+86157730 | MsG0180005045.01.T01:CDS |
| AGCGAACACCTTCTCCAGCT+GGG | 0.486800 | 1:+86157731 | MsG0180005045.01.T01:CDS |
| TCCTGCGTTACCGCCTCAAA+TGG | 0.501071 | 1:+86157861 | MsG0180005045.01.T01:CDS |
| CCTTTGCCTTTCGAATTCAT+GGG | 0.505515 | 1:+86157443 | MsG0180005045.01.T01:CDS |
| ACCATTTGAGGCGGTAACGC+AGG | 0.520151 | 1:-86157862 | None:intergenic |
| CAGCATCGTGGGTGATCGGT+TGG | 0.552905 | 1:+86157784 | MsG0180005045.01.T01:CDS |
| AGAGCAGCATCGTGGGTGAT+CGG | 0.565902 | 1:+86157780 | MsG0180005045.01.T01:CDS |
| AGCATGATAAAAGAAATTCA+AGG | 0.569476 | 1:-86157966 | None:intergenic |
| AAGAGTGAGAGCAGCATCGT+GGG | 0.573141 | 1:+86157773 | MsG0180005045.01.T01:CDS |
| TATTGTCAGGGTCACCTGAA+AGG | 0.575314 | 1:-86157627 | None:intergenic |
| CTATGAGACAGTATAATTCG+AGG | 0.577165 | 1:-86157559 | None:intergenic |
| CAACAATCTTCAGAAGGAGG+AGG | 0.580609 | 1:-86157527 | None:intergenic |
| TTTGAGCTTTCGTATTGTCA+GGG | 0.585321 | 1:-86157639 | None:intergenic |
| TTCCAGCACCTTATGCGAGA+TGG | 0.591021 | 1:+86157413 | MsG0180005045.01.T01:CDS |
| CCCATGAATTCGAAAGGCAA+AGG | 0.595552 | 1:-86157443 | None:intergenic |
| GACTCGCAACAATCTTCAGA+AGG | 0.595638 | 1:-86157533 | None:intergenic |
| TTATCGCCCATGAATTCGAA+AGG | 0.600486 | 1:-86157449 | None:intergenic |
| GCGAACACCTTCTCCAGCTG+GGG | 0.603030 | 1:+86157732 | MsG0180005045.01.T01:CDS |
| CACCTTATGCGAGATGGCGA+AGG | 0.605631 | 1:+86157419 | MsG0180005045.01.T01:CDS |
| GGAGACATGCAGCAGAGAGG+TGG | 0.612152 | 1:+86157815 | MsG0180005045.01.T01:CDS |
| TATTGGCTATGAGACTCTCG+AGG | 0.631466 | 1:-86157694 | None:intergenic |
| TCGCAACAATCTTCAGAAGG+AGG | 0.652776 | 1:-86157530 | None:intergenic |
| GTTTCTCGAACCATTTGAGG+CGG | 0.659651 | 1:-86157871 | None:intergenic |
| GAAGAGTGAGAGCAGCATCG+TGG | 0.660813 | 1:+86157772 | MsG0180005045.01.T01:CDS |
| CGAACACCTTCTCCAGCTGG+GGG | 0.680093 | 1:+86157733 | MsG0180005045.01.T01:CDS |
| ATTGGCTATGAGACTCTCGA+GGG | 0.685902 | 1:-86157693 | None:intergenic |
| TTCGGAGACATGCAGCAGAG+AGG | 0.705215 | 1:+86157812 | MsG0180005045.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCAATAATTTCCAAGAT+TGG | - | Chr1:86157602-86157621 | None:intergenic | 20.0% |
| ! | AGCATGATAAAAGAAATTCA+AGG | - | Chr1:86157969-86157988 | None:intergenic | 25.0% |
| !!! | GTCGAAAAGATCTAAAATTT+TGG | + | Chr1:86157382-86157401 | MsG0180005045.01.T01:CDS | 25.0% |
| AAAGAAATTCAAGGAGTTTC+TGG | - | Chr1:86157960-86157979 | None:intergenic | 30.0% | |
| CAATAAACTCTGCACAAAAA+CGG | - | Chr1:86157481-86157500 | None:intergenic | 30.0% | |
| GCTGTTGTATTGTAGATTAT+TGG | - | Chr1:86157714-86157733 | None:intergenic | 30.0% | |
| TTGCAGCTATTATTGAAAAG+AGG | + | Chr1:86157918-86157937 | MsG0180005045.01.T01:CDS | 30.0% | |
| ! | TATTGATTACTTGTCCTTTC+AGG | + | Chr1:86157613-86157632 | MsG0180005045.01.T01:CDS | 30.0% |
| AAGCTCAAATTGGCTAAGTT+TGG | + | Chr1:86157653-86157672 | MsG0180005045.01.T01:CDS | 35.0% | |
| ATTTGAGCTTTCGTATTGTC+AGG | - | Chr1:86157643-86157662 | None:intergenic | 35.0% | |
| CTATGAGACAGTATAATTCG+AGG | - | Chr1:86157562-86157581 | None:intergenic | 35.0% | |
| GACAATACGAAAGCTCAAAT+TGG | + | Chr1:86157643-86157662 | MsG0180005045.01.T01:CDS | 35.0% | |
| TTCAATAATAGCTGCAAGCT+CGG | - | Chr1:86157915-86157934 | None:intergenic | 35.0% | |
| TTTGAGCTTTCGTATTGTCA+GGG | - | Chr1:86157642-86157661 | None:intergenic | 35.0% | |
| AAGGAGGAGGAAGAGTTATT+TGG | - | Chr1:86157517-86157536 | None:intergenic | 40.0% | |
| CCTTTGCCTTTCGAATTCAT+GGG | + | Chr1:86157443-86157462 | MsG0180005045.01.T01:CDS | 40.0% | |
| CTCAAATTGGCTAAGTTTGG+TGG | + | Chr1:86157656-86157675 | MsG0180005045.01.T01:CDS | 40.0% | |
| TCCTTTGCCTTTCGAATTCA+TGG | + | Chr1:86157442-86157461 | MsG0180005045.01.T01:CDS | 40.0% | |
| TTATCGCCCATGAATTCGAA+AGG | - | Chr1:86157452-86157471 | None:intergenic | 40.0% | |
| ATTGGCTATGAGACTCTCGA+GGG | - | Chr1:86157696-86157715 | None:intergenic | 45.0% | |
| CAACAATCTTCAGAAGGAGG+AGG | - | Chr1:86157530-86157549 | None:intergenic | 45.0% | |
| CCCATGAATTCGAAAGGCAA+AGG | - | Chr1:86157446-86157465 | None:intergenic | 45.0% | |
| CCTCAAATGGTTCGAGAAAC+AGG | + | Chr1:86157874-86157893 | MsG0180005045.01.T01:CDS | 45.0% | |
| GACTCGCAACAATCTTCAGA+AGG | - | Chr1:86157536-86157555 | None:intergenic | 45.0% | |
| GTTTCTCGAACCATTTGAGG+CGG | - | Chr1:86157874-86157893 | None:intergenic | 45.0% | |
| TATTGTCAGGGTCACCTGAA+AGG | - | Chr1:86157630-86157649 | None:intergenic | 45.0% | |
| TCGCAACAATCTTCAGAAGG+AGG | - | Chr1:86157533-86157552 | None:intergenic | 45.0% | |
| TCTTCGCAGACTTCCAATCT+TGG | + | Chr1:86157586-86157605 | MsG0180005045.01.T01:CDS | 45.0% | |
| ! | CCTGTTTCTCGAACCATTTG+AGG | - | Chr1:86157877-86157896 | None:intergenic | 45.0% |
| ! | TATTGGCTATGAGACTCTCG+AGG | - | Chr1:86157697-86157716 | None:intergenic | 45.0% |
| ! | TGGCTAAGTTTGGTGGATCT+TGG | + | Chr1:86157663-86157682 | MsG0180005045.01.T01:CDS | 45.0% |
| !! | GGTGATCGGTTGGTGATATT+CGG | + | Chr1:86157794-86157813 | MsG0180005045.01.T01:CDS | 45.0% |
| AAGAGTGAGAGCAGCATCGT+GGG | + | Chr1:86157773-86157792 | MsG0180005045.01.T01:CDS | 50.0% | |
| TCTTGGCACGCTCACTAGTA+AGG | - | Chr1:86157841-86157860 | None:intergenic | 50.0% | |
| TTCCAGCACCTTATGCGAGA+TGG | + | Chr1:86157413-86157432 | MsG0180005045.01.T01:CDS | 50.0% | |
| AGAGCAGCATCGTGGGTGAT+CGG | + | Chr1:86157780-86157799 | MsG0180005045.01.T01:CDS | 55.0% | |
| AGCGAACACCTTCTCCAGCT+GGG | + | Chr1:86157731-86157750 | MsG0180005045.01.T01:CDS | 55.0% | |
| CACCTTATGCGAGATGGCGA+AGG | + | Chr1:86157419-86157438 | MsG0180005045.01.T01:CDS | 55.0% | |
| GAAGAGTGAGAGCAGCATCG+TGG | + | Chr1:86157772-86157791 | MsG0180005045.01.T01:CDS | 55.0% | |
| GACCTTCGCCATCTCGCATA+AGG | - | Chr1:86157424-86157443 | None:intergenic | 55.0% | |
| TCCTGCGTTACCGCCTCAAA+TGG | + | Chr1:86157861-86157880 | MsG0180005045.01.T01:CDS | 55.0% | |
| TTCGGAGACATGCAGCAGAG+AGG | + | Chr1:86157812-86157831 | MsG0180005045.01.T01:CDS | 55.0% | |
| ! | ACCATTTGAGGCGGTAACGC+AGG | - | Chr1:86157865-86157884 | None:intergenic | 55.0% |
| ! | CATTTTCTCGATCCCCCAGC+TGG | - | Chr1:86157748-86157767 | None:intergenic | 55.0% |
| CAGCATCGTGGGTGATCGGT+TGG | + | Chr1:86157784-86157803 | MsG0180005045.01.T01:CDS | 60.0% | |
| CAGCGAACACCTTCTCCAGC+TGG | + | Chr1:86157730-86157749 | MsG0180005045.01.T01:CDS | 60.0% | |
| CGAACACCTTCTCCAGCTGG+GGG | + | Chr1:86157733-86157752 | MsG0180005045.01.T01:CDS | 60.0% | |
| CGCCATCTCGCATAAGGTGC+TGG | - | Chr1:86157418-86157437 | None:intergenic | 60.0% | |
| GAGGCGGTAACGCAGGATCT+TGG | - | Chr1:86157858-86157877 | None:intergenic | 60.0% | |
| GCGAACACCTTCTCCAGCTG+GGG | + | Chr1:86157732-86157751 | MsG0180005045.01.T01:CDS | 60.0% | |
| GGAGACATGCAGCAGAGAGG+TGG | + | Chr1:86157815-86157834 | MsG0180005045.01.T01:CDS | 60.0% | |
| CTCGATCCCCCAGCTGGAGA+AGG | - | Chr1:86157742-86157761 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 86157380 | 86157991 | 86157380 | ID=MsG0180005045.01;Name=MsG0180005045.01 |
| Chr1 | mRNA | 86157380 | 86157991 | 86157380 | ID=MsG0180005045.01.T01;Parent=MsG0180005045.01;Name=MsG0180005045.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|203 |
| Chr1 | exon | 86157380 | 86157991 | 86157380 | ID=MsG0180005045.01.T01:exon:36863;Parent=MsG0180005045.01.T01 |
| Chr1 | CDS | 86157380 | 86157991 | 86157380 | ID=MsG0180005045.01.T01:cds;Parent=MsG0180005045.01.T01 |
| Gene Sequence |
| Protein sequence |
