Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048221.01.T01 | GAU12760.1 | 39.024 | 205 | 121 | 2 | 1 | 201 | 1 | 205 | 7.71E-34 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048221.01 | MsG0080048224.01 | 0.994169 | 4.198658e-205 | 4.324793e-198 |
| MsG0080048221.01 | MsG0180004599.01 | 0.806653 | 7.410092e-50 | 4.043858e-47 |
| MsG0080048221.01 | MsG0180005045.01 | 0.974439 | 3.699784e-138 | 5.122637e-132 |
| MsG0080048221.01 | MsG0180005960.01 | 0.814315 | 1.639847e-51 | 1.096715e-48 |
| MsG0080048221.01 | MsG0280009350.01 | 0.812771 | 3.584472e-51 | 2.299039e-48 |
| MsG0080048221.01 | MsG0380011895.01 | 0.811024 | 8.610102e-51 | 5.272189e-48 |
| MsG0080048221.01 | MsG0380016182.01 | 0.820709 | 5.944233e-53 | 4.738665e-50 |
| MsG0080048221.01 | MsG0480018473.01 | 0.817277 | 3.583669e-52 | 2.598238e-49 |
| MsG0080048221.01 | MsG0480021959.01 | 0.810058 | 1.392183e-50 | 8.308870e-48 |
| MsG0080048221.01 | MsG0480022933.01 | 0.823468 | 1.363020e-53 | 1.174561e-50 |
| MsG0080048221.01 | MsG0780036117.01 | 0.812032 | 5.198821e-51 | 3.269208e-48 |
| MsG0080048221.01 | MsG0780038092.01 | 0.803002 | 4.291780e-49 | 2.132613e-46 |
| MsG0080048221.01 | MsG0880042743.01 | 0.824780 | 6.704401e-54 | 5.997753e-51 |
| MsG0080048221.01 | MsG0880043804.01 | 0.815706 | 8.055888e-52 | 5.595370e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAATTCAAGGAGTTTC+TGG | 0.123756 | contig246end:+41515 | None:intergenic |
| TATTGATTACTTGTCCTTTC+AGG | 0.165047 | contig246end:-41859 | MsG0080048221.01.T01:CDS |
| GTCGAAAAGATCTAAAATTT+TGG | 0.178634 | contig246end:-42090 | MsG0080048221.01.T01:CDS |
| TCCTTTGCCTTTCGAATTCA+TGG | 0.301017 | contig246end:-42030 | MsG0080048221.01.T01:CDS |
| GCTGTTGTATTGTAGATTAT+TGG | 0.305986 | contig246end:+41761 | None:intergenic |
| GACAATACGAAAGCTCAAAT+TGG | 0.319470 | contig246end:-41829 | MsG0080048221.01.T01:CDS |
| AAGGAGGAGGAAGAGTTATT+TGG | 0.323852 | contig246end:+41958 | None:intergenic |
| CCTCAAATGGTTCGAGAAAC+AGG | 0.344909 | contig246end:-41598 | MsG0080048221.01.T01:CDS |
| TAATCAATAATTTCCAAGAT+TGG | 0.354103 | contig246end:+41873 | None:intergenic |
| AAGCTCAAATTGGCTAAGTT+TGG | 0.359836 | contig246end:-41819 | MsG0080048221.01.T01:CDS |
| ATTTGAGCTTTCGTATTGTC+AGG | 0.369492 | contig246end:+41832 | None:intergenic |
| TCTTCGCAGACTTCCAATCT+TGG | 0.389719 | contig246end:-41886 | MsG0080048221.01.T01:CDS |
| TGGCTAAGTTTGGTGGATCT+TGG | 0.390395 | contig246end:-41809 | MsG0080048221.01.T01:CDS |
| CCTGTTTCTCGAACCATTTG+AGG | 0.415023 | contig246end:+41598 | None:intergenic |
| CGCCATCTCGCATAAGGTGC+TGG | 0.424742 | contig246end:+42057 | None:intergenic |
| CTCAAATTGGCTAAGTTTGG+TGG | 0.431809 | contig246end:-41816 | MsG0080048221.01.T01:CDS |
| GGTGATCGGTTGGTGATATT+CGG | 0.443933 | contig246end:-41678 | MsG0080048221.01.T01:CDS |
| GAGGCGGTAACGCAGGATCT+TGG | 0.445121 | contig246end:+41617 | None:intergenic |
| GACCTTCGCCATCTCGCATA+AGG | 0.463108 | contig246end:+42051 | None:intergenic |
| TCTTGGCACGCTCACTAGTA+AGG | 0.467933 | contig246end:+41634 | None:intergenic |
| TTGCAGCTATTATTGAAAAG+AGG | 0.472869 | contig246end:-41554 | MsG0080048221.01.T01:CDS |
| TTCAATAATAGCTGCAAGCT+CGG | 0.476416 | contig246end:+41560 | None:intergenic |
| CAGCGAACACCTTCTCCAGC+TGG | 0.484890 | contig246end:-41742 | MsG0080048221.01.T01:CDS |
| AGCGAACACCTTCTCCAGCT+GGG | 0.486800 | contig246end:-41741 | MsG0080048221.01.T01:CDS |
| TCCTGCGTTACCGCCTCAAA+TGG | 0.501071 | contig246end:-41611 | MsG0080048221.01.T01:CDS |
| CCTTTGCCTTTCGAATTCAT+GGG | 0.505515 | contig246end:-42029 | MsG0080048221.01.T01:CDS |
| ACCATTTGAGGCGGTAACGC+AGG | 0.520151 | contig246end:+41610 | None:intergenic |
| CAGCATCGTGGGTGATCGGT+TGG | 0.552905 | contig246end:-41688 | MsG0080048221.01.T01:CDS |
| AGAGCAGCATCGTGGGTGAT+CGG | 0.565902 | contig246end:-41692 | MsG0080048221.01.T01:CDS |
| AGCATGATAAAAGAAATTCA+AGG | 0.569476 | contig246end:+41506 | None:intergenic |
| AAGAGTGAGAGCAGCATCGT+GGG | 0.573141 | contig246end:-41699 | MsG0080048221.01.T01:CDS |
| TATTGTCAGGGTCACCTGAA+AGG | 0.575314 | contig246end:+41845 | None:intergenic |
| CTATGAGACAGTATAATTCG+AGG | 0.577165 | contig246end:+41913 | None:intergenic |
| CAACAATCTTCAGAAGGAGG+AGG | 0.580609 | contig246end:+41945 | None:intergenic |
| TTTGAGCTTTCGTATTGTCA+GGG | 0.585321 | contig246end:+41833 | None:intergenic |
| TTCCAGCACCTTATGCGAGA+TGG | 0.591021 | contig246end:-42059 | MsG0080048221.01.T01:CDS |
| CCCATGAATTCGAAAGGCAA+AGG | 0.595552 | contig246end:+42029 | None:intergenic |
| GACTCGCAACAATCTTCAGA+AGG | 0.595638 | contig246end:+41939 | None:intergenic |
| TTATCGCCCATGAATTCGAA+AGG | 0.600486 | contig246end:+42023 | None:intergenic |
| GCGAACACCTTCTCCAGCTG+GGG | 0.603030 | contig246end:-41740 | MsG0080048221.01.T01:CDS |
| CACCTTATGCGAGATGGCGA+AGG | 0.605631 | contig246end:-42053 | MsG0080048221.01.T01:CDS |
| GGAGACATGCAGCAGAGAGG+TGG | 0.612152 | contig246end:-41657 | MsG0080048221.01.T01:CDS |
| TATTGGCTATGAGACTCTCG+AGG | 0.631466 | contig246end:+41778 | None:intergenic |
| TCGCAACAATCTTCAGAAGG+AGG | 0.652776 | contig246end:+41942 | None:intergenic |
| GTTTCTCGAACCATTTGAGG+CGG | 0.659651 | contig246end:+41601 | None:intergenic |
| GAAGAGTGAGAGCAGCATCG+TGG | 0.660813 | contig246end:-41700 | MsG0080048221.01.T01:CDS |
| CGAACACCTTCTCCAGCTGG+GGG | 0.680093 | contig246end:-41739 | MsG0080048221.01.T01:CDS |
| ATTGGCTATGAGACTCTCGA+GGG | 0.685902 | contig246end:+41779 | None:intergenic |
| TTCGGAGACATGCAGCAGAG+AGG | 0.705215 | contig246end:-41660 | MsG0080048221.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCAATAATTTCCAAGAT+TGG | + | contig246end:41725-41744 | None:intergenic | 20.0% |
| ! | AGCATGATAAAAGAAATTCA+AGG | + | contig246end:42092-42111 | None:intergenic | 25.0% |
| !!! | GTCGAAAAGATCTAAAATTT+TGG | - | contig246end:41505-41524 | MsG0080048221.01.T01:CDS | 25.0% |
| AAAGAAATTCAAGGAGTTTC+TGG | + | contig246end:42083-42102 | None:intergenic | 30.0% | |
| CAATAAACTCTGCACAAAAA+CGG | + | contig246end:41604-41623 | None:intergenic | 30.0% | |
| GCTGTTGTATTGTAGATTAT+TGG | + | contig246end:41837-41856 | None:intergenic | 30.0% | |
| TTGCAGCTATTATTGAAAAG+AGG | - | contig246end:42041-42060 | MsG0080048221.01.T01:CDS | 30.0% | |
| ! | TATTGATTACTTGTCCTTTC+AGG | - | contig246end:41736-41755 | MsG0080048221.01.T01:CDS | 30.0% |
| AAGCTCAAATTGGCTAAGTT+TGG | - | contig246end:41776-41795 | MsG0080048221.01.T01:CDS | 35.0% | |
| ATTTGAGCTTTCGTATTGTC+AGG | + | contig246end:41766-41785 | None:intergenic | 35.0% | |
| CTATGAGACAGTATAATTCG+AGG | + | contig246end:41685-41704 | None:intergenic | 35.0% | |
| GACAATACGAAAGCTCAAAT+TGG | - | contig246end:41766-41785 | MsG0080048221.01.T01:CDS | 35.0% | |
| TTCAATAATAGCTGCAAGCT+CGG | + | contig246end:42038-42057 | None:intergenic | 35.0% | |
| TTTGAGCTTTCGTATTGTCA+GGG | + | contig246end:41765-41784 | None:intergenic | 35.0% | |
| AAGGAGGAGGAAGAGTTATT+TGG | + | contig246end:41640-41659 | None:intergenic | 40.0% | |
| CCTTTGCCTTTCGAATTCAT+GGG | - | contig246end:41566-41585 | MsG0080048221.01.T01:CDS | 40.0% | |
| CTCAAATTGGCTAAGTTTGG+TGG | - | contig246end:41779-41798 | MsG0080048221.01.T01:CDS | 40.0% | |
| TCCTTTGCCTTTCGAATTCA+TGG | - | contig246end:41565-41584 | MsG0080048221.01.T01:CDS | 40.0% | |
| TTATCGCCCATGAATTCGAA+AGG | + | contig246end:41575-41594 | None:intergenic | 40.0% | |
| ATTGGCTATGAGACTCTCGA+GGG | + | contig246end:41819-41838 | None:intergenic | 45.0% | |
| CAACAATCTTCAGAAGGAGG+AGG | + | contig246end:41653-41672 | None:intergenic | 45.0% | |
| CCCATGAATTCGAAAGGCAA+AGG | + | contig246end:41569-41588 | None:intergenic | 45.0% | |
| CCTCAAATGGTTCGAGAAAC+AGG | - | contig246end:41997-42016 | MsG0080048221.01.T01:CDS | 45.0% | |
| GACTCGCAACAATCTTCAGA+AGG | + | contig246end:41659-41678 | None:intergenic | 45.0% | |
| GTTTCTCGAACCATTTGAGG+CGG | + | contig246end:41997-42016 | None:intergenic | 45.0% | |
| TATTGTCAGGGTCACCTGAA+AGG | + | contig246end:41753-41772 | None:intergenic | 45.0% | |
| TCGCAACAATCTTCAGAAGG+AGG | + | contig246end:41656-41675 | None:intergenic | 45.0% | |
| TCTTCGCAGACTTCCAATCT+TGG | - | contig246end:41709-41728 | MsG0080048221.01.T01:CDS | 45.0% | |
| ! | CCTGTTTCTCGAACCATTTG+AGG | + | contig246end:42000-42019 | None:intergenic | 45.0% |
| ! | TATTGGCTATGAGACTCTCG+AGG | + | contig246end:41820-41839 | None:intergenic | 45.0% |
| ! | TGGCTAAGTTTGGTGGATCT+TGG | - | contig246end:41786-41805 | MsG0080048221.01.T01:CDS | 45.0% |
| !! | GGTGATCGGTTGGTGATATT+CGG | - | contig246end:41917-41936 | MsG0080048221.01.T01:CDS | 45.0% |
| AAGAGTGAGAGCAGCATCGT+GGG | - | contig246end:41896-41915 | MsG0080048221.01.T01:CDS | 50.0% | |
| TCTTGGCACGCTCACTAGTA+AGG | + | contig246end:41964-41983 | None:intergenic | 50.0% | |
| TTCCAGCACCTTATGCGAGA+TGG | - | contig246end:41536-41555 | MsG0080048221.01.T01:CDS | 50.0% | |
| AGAGCAGCATCGTGGGTGAT+CGG | - | contig246end:41903-41922 | MsG0080048221.01.T01:CDS | 55.0% | |
| AGCGAACACCTTCTCCAGCT+GGG | - | contig246end:41854-41873 | MsG0080048221.01.T01:CDS | 55.0% | |
| CACCTTATGCGAGATGGCGA+AGG | - | contig246end:41542-41561 | MsG0080048221.01.T01:CDS | 55.0% | |
| GAAGAGTGAGAGCAGCATCG+TGG | - | contig246end:41895-41914 | MsG0080048221.01.T01:CDS | 55.0% | |
| GACCTTCGCCATCTCGCATA+AGG | + | contig246end:41547-41566 | None:intergenic | 55.0% | |
| TCCTGCGTTACCGCCTCAAA+TGG | - | contig246end:41984-42003 | MsG0080048221.01.T01:CDS | 55.0% | |
| TTCGGAGACATGCAGCAGAG+AGG | - | contig246end:41935-41954 | MsG0080048221.01.T01:CDS | 55.0% | |
| ! | ACCATTTGAGGCGGTAACGC+AGG | + | contig246end:41988-42007 | None:intergenic | 55.0% |
| ! | CATTTTCTCGATCCCCCAGC+TGG | + | contig246end:41871-41890 | None:intergenic | 55.0% |
| CAGCATCGTGGGTGATCGGT+TGG | - | contig246end:41907-41926 | MsG0080048221.01.T01:CDS | 60.0% | |
| CAGCGAACACCTTCTCCAGC+TGG | - | contig246end:41853-41872 | MsG0080048221.01.T01:CDS | 60.0% | |
| CGAACACCTTCTCCAGCTGG+GGG | - | contig246end:41856-41875 | MsG0080048221.01.T01:CDS | 60.0% | |
| CGCCATCTCGCATAAGGTGC+TGG | + | contig246end:41541-41560 | None:intergenic | 60.0% | |
| GAGGCGGTAACGCAGGATCT+TGG | + | contig246end:41981-42000 | None:intergenic | 60.0% | |
| GCGAACACCTTCTCCAGCTG+GGG | - | contig246end:41855-41874 | MsG0080048221.01.T01:CDS | 60.0% | |
| GGAGACATGCAGCAGAGAGG+TGG | - | contig246end:41938-41957 | MsG0080048221.01.T01:CDS | 60.0% | |
| CTCGATCCCCCAGCTGGAGA+AGG | + | contig246end:41865-41884 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig246end | gene | 41503 | 42114 | 41503 | ID=MsG0080048221.01;Name=MsG0080048221.01 |
| contig246end | mRNA | 41503 | 42114 | 41503 | ID=MsG0080048221.01.T01;Parent=MsG0080048221.01;Name=MsG0080048221.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|203 |
| contig246end | exon | 41503 | 42114 | 41503 | ID=MsG0080048221.01.T01:exon:8443;Parent=MsG0080048221.01.T01 |
| contig246end | CDS | 41503 | 42114 | 41503 | ID=MsG0080048221.01.T01:cds;Parent=MsG0080048221.01.T01 |
| Gene Sequence |
| Protein sequence |
