Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048224.01.T01 | GAU12760.1 | 39.024 | 205 | 121 | 2 | 1 | 201 | 1 | 205 | 7.71E-34 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048221.01 | MsG0080048224.01 | 0.994169 | 4.198658e-205 | 4.324793e-198 |
| MsG0080048224.01 | MsG0180005045.01 | 0.981647 | 4.215371e-153 | 1.119424e-146 |
| MsG0080048224.01 | MsG0180005960.01 | 0.810256 | 1.261656e-50 | 7.568743e-48 |
| MsG0080048224.01 | MsG0280009350.01 | 0.806571 | 7.711333e-50 | 4.199720e-47 |
| MsG0080048224.01 | MsG0380016182.01 | 0.810717 | 1.003152e-50 | 6.092308e-48 |
| MsG0080048224.01 | MsG0480018473.01 | 0.813320 | 2.717585e-51 | 1.769019e-48 |
| MsG0080048224.01 | MsG0480021959.01 | 0.806012 | 1.011155e-49 | 5.429206e-47 |
| MsG0080048224.01 | MsG0480022933.01 | 0.818264 | 2.146580e-52 | 1.598990e-49 |
| MsG0080048224.01 | MsG0780036117.01 | 0.803853 | 2.858910e-49 | 1.452123e-46 |
| MsG0080048224.01 | MsG0880042743.01 | 0.817862 | 2.645937e-52 | 1.949452e-49 |
| MsG0080048224.01 | MsG0880043804.01 | 0.805229 | 1.476486e-49 | 7.769431e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAATTCAAGGAGTTTC+TGG | 0.123756 | contig246end:+97178 | None:intergenic |
| TATTGATTACTTGTCCTTTC+AGG | 0.165047 | contig246end:-97522 | MsG0080048224.01.T01:CDS |
| GTCGAAAAGATCTAAAATTT+TGG | 0.178634 | contig246end:-97753 | MsG0080048224.01.T01:CDS |
| TCCTTTGCCTTTCGAATTCA+TGG | 0.301017 | contig246end:-97693 | MsG0080048224.01.T01:CDS |
| GCTGTTGTATTGTAGATTAT+TGG | 0.305986 | contig246end:+97424 | None:intergenic |
| GACAATACGAAAGCTCAAAT+TGG | 0.319470 | contig246end:-97492 | MsG0080048224.01.T01:CDS |
| AAGGAGGAGGAAGAGTTATT+TGG | 0.323852 | contig246end:+97621 | None:intergenic |
| CCTCAAATGGTTCGAGAAAC+AGG | 0.344909 | contig246end:-97261 | MsG0080048224.01.T01:CDS |
| TAATCAATAATTTCCAAGAT+TGG | 0.354103 | contig246end:+97536 | None:intergenic |
| AAGCTCAAATTGGCTAAGTT+TGG | 0.359836 | contig246end:-97482 | MsG0080048224.01.T01:CDS |
| ATTTGAGCTTTCGTATTGTC+AGG | 0.369492 | contig246end:+97495 | None:intergenic |
| TCTTCGCAGACTTCCAATCT+TGG | 0.389719 | contig246end:-97549 | MsG0080048224.01.T01:CDS |
| TGGCTAAGTTTGGTGGATCT+TGG | 0.390395 | contig246end:-97472 | MsG0080048224.01.T01:CDS |
| CCTGTTTCTCGAACCATTTG+AGG | 0.415023 | contig246end:+97261 | None:intergenic |
| CGCCATCTCGCATAAGGTGC+TGG | 0.424742 | contig246end:+97720 | None:intergenic |
| CTCAAATTGGCTAAGTTTGG+TGG | 0.431809 | contig246end:-97479 | MsG0080048224.01.T01:CDS |
| GGTGATCGGTTGGTGATATT+CGG | 0.443933 | contig246end:-97341 | MsG0080048224.01.T01:CDS |
| GAGGCGGTAACGCAGGATCT+TGG | 0.445121 | contig246end:+97280 | None:intergenic |
| GACCTTCGCCATCTCGCATA+AGG | 0.463108 | contig246end:+97714 | None:intergenic |
| TCTTGGCACGCTCACTAGTA+AGG | 0.467933 | contig246end:+97297 | None:intergenic |
| TTGCAGCTATTATTGAAAAG+AGG | 0.472869 | contig246end:-97217 | MsG0080048224.01.T01:CDS |
| TTCAATAATAGCTGCAAGCT+CGG | 0.476416 | contig246end:+97223 | None:intergenic |
| CAGCGAACACCTTCTCCAGC+TGG | 0.484890 | contig246end:-97405 | MsG0080048224.01.T01:CDS |
| AGCGAACACCTTCTCCAGCT+GGG | 0.486800 | contig246end:-97404 | MsG0080048224.01.T01:CDS |
| TCCTGCGTTACCGCCTCAAA+TGG | 0.501071 | contig246end:-97274 | MsG0080048224.01.T01:CDS |
| CCTTTGCCTTTCGAATTCAT+GGG | 0.505515 | contig246end:-97692 | MsG0080048224.01.T01:CDS |
| ACCATTTGAGGCGGTAACGC+AGG | 0.520151 | contig246end:+97273 | None:intergenic |
| CAGCATCGTGGGTGATCGGT+TGG | 0.552905 | contig246end:-97351 | MsG0080048224.01.T01:CDS |
| AGAGCAGCATCGTGGGTGAT+CGG | 0.565902 | contig246end:-97355 | MsG0080048224.01.T01:CDS |
| AGCATGATAAAAGAAATTCA+AGG | 0.569476 | contig246end:+97169 | None:intergenic |
| AAGAGTGAGAGCAGCATCGT+GGG | 0.573141 | contig246end:-97362 | MsG0080048224.01.T01:CDS |
| TATTGTCAGGGTCACCTGAA+AGG | 0.575314 | contig246end:+97508 | None:intergenic |
| CTATGAGACAGTATAATTCG+AGG | 0.577165 | contig246end:+97576 | None:intergenic |
| CAACAATCTTCAGAAGGAGG+AGG | 0.580609 | contig246end:+97608 | None:intergenic |
| TTTGAGCTTTCGTATTGTCA+GGG | 0.585321 | contig246end:+97496 | None:intergenic |
| TTCCAGCACCTTATGCGAGA+TGG | 0.591021 | contig246end:-97722 | MsG0080048224.01.T01:CDS |
| CCCATGAATTCGAAAGGCAA+AGG | 0.595552 | contig246end:+97692 | None:intergenic |
| GACTCGCAACAATCTTCAGA+AGG | 0.595638 | contig246end:+97602 | None:intergenic |
| TTATCGCCCATGAATTCGAA+AGG | 0.600486 | contig246end:+97686 | None:intergenic |
| GCGAACACCTTCTCCAGCTG+GGG | 0.603030 | contig246end:-97403 | MsG0080048224.01.T01:CDS |
| CACCTTATGCGAGATGGCGA+AGG | 0.605631 | contig246end:-97716 | MsG0080048224.01.T01:CDS |
| GGAGACATGCAGCAGAGAGG+TGG | 0.612152 | contig246end:-97320 | MsG0080048224.01.T01:CDS |
| TATTGGCTATGAGACTCTCG+AGG | 0.631466 | contig246end:+97441 | None:intergenic |
| TCGCAACAATCTTCAGAAGG+AGG | 0.652776 | contig246end:+97605 | None:intergenic |
| GTTTCTCGAACCATTTGAGG+CGG | 0.659651 | contig246end:+97264 | None:intergenic |
| GAAGAGTGAGAGCAGCATCG+TGG | 0.660813 | contig246end:-97363 | MsG0080048224.01.T01:CDS |
| CGAACACCTTCTCCAGCTGG+GGG | 0.680093 | contig246end:-97402 | MsG0080048224.01.T01:CDS |
| ATTGGCTATGAGACTCTCGA+GGG | 0.685902 | contig246end:+97442 | None:intergenic |
| TTCGGAGACATGCAGCAGAG+AGG | 0.705215 | contig246end:-97323 | MsG0080048224.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCAATAATTTCCAAGAT+TGG | + | contig246end:97388-97407 | None:intergenic | 20.0% |
| ! | AGCATGATAAAAGAAATTCA+AGG | + | contig246end:97755-97774 | None:intergenic | 25.0% |
| !!! | GTCGAAAAGATCTAAAATTT+TGG | - | contig246end:97168-97187 | MsG0080048224.01.T01:CDS | 25.0% |
| AAAGAAATTCAAGGAGTTTC+TGG | + | contig246end:97746-97765 | None:intergenic | 30.0% | |
| CAATAAACTCTGCACAAAAA+CGG | + | contig246end:97267-97286 | None:intergenic | 30.0% | |
| GCTGTTGTATTGTAGATTAT+TGG | + | contig246end:97500-97519 | None:intergenic | 30.0% | |
| TTGCAGCTATTATTGAAAAG+AGG | - | contig246end:97704-97723 | MsG0080048224.01.T01:CDS | 30.0% | |
| ! | TATTGATTACTTGTCCTTTC+AGG | - | contig246end:97399-97418 | MsG0080048224.01.T01:CDS | 30.0% |
| AAGCTCAAATTGGCTAAGTT+TGG | - | contig246end:97439-97458 | MsG0080048224.01.T01:CDS | 35.0% | |
| ATTTGAGCTTTCGTATTGTC+AGG | + | contig246end:97429-97448 | None:intergenic | 35.0% | |
| CTATGAGACAGTATAATTCG+AGG | + | contig246end:97348-97367 | None:intergenic | 35.0% | |
| GACAATACGAAAGCTCAAAT+TGG | - | contig246end:97429-97448 | MsG0080048224.01.T01:CDS | 35.0% | |
| TTCAATAATAGCTGCAAGCT+CGG | + | contig246end:97701-97720 | None:intergenic | 35.0% | |
| TTTGAGCTTTCGTATTGTCA+GGG | + | contig246end:97428-97447 | None:intergenic | 35.0% | |
| AAGGAGGAGGAAGAGTTATT+TGG | + | contig246end:97303-97322 | None:intergenic | 40.0% | |
| CCTTTGCCTTTCGAATTCAT+GGG | - | contig246end:97229-97248 | MsG0080048224.01.T01:CDS | 40.0% | |
| CTCAAATTGGCTAAGTTTGG+TGG | - | contig246end:97442-97461 | MsG0080048224.01.T01:CDS | 40.0% | |
| TCCTTTGCCTTTCGAATTCA+TGG | - | contig246end:97228-97247 | MsG0080048224.01.T01:CDS | 40.0% | |
| TTATCGCCCATGAATTCGAA+AGG | + | contig246end:97238-97257 | None:intergenic | 40.0% | |
| ATTGGCTATGAGACTCTCGA+GGG | + | contig246end:97482-97501 | None:intergenic | 45.0% | |
| CAACAATCTTCAGAAGGAGG+AGG | + | contig246end:97316-97335 | None:intergenic | 45.0% | |
| CCCATGAATTCGAAAGGCAA+AGG | + | contig246end:97232-97251 | None:intergenic | 45.0% | |
| CCTCAAATGGTTCGAGAAAC+AGG | - | contig246end:97660-97679 | MsG0080048224.01.T01:CDS | 45.0% | |
| GACTCGCAACAATCTTCAGA+AGG | + | contig246end:97322-97341 | None:intergenic | 45.0% | |
| GTTTCTCGAACCATTTGAGG+CGG | + | contig246end:97660-97679 | None:intergenic | 45.0% | |
| TATTGTCAGGGTCACCTGAA+AGG | + | contig246end:97416-97435 | None:intergenic | 45.0% | |
| TCGCAACAATCTTCAGAAGG+AGG | + | contig246end:97319-97338 | None:intergenic | 45.0% | |
| TCTTCGCAGACTTCCAATCT+TGG | - | contig246end:97372-97391 | MsG0080048224.01.T01:CDS | 45.0% | |
| ! | CCTGTTTCTCGAACCATTTG+AGG | + | contig246end:97663-97682 | None:intergenic | 45.0% |
| ! | TATTGGCTATGAGACTCTCG+AGG | + | contig246end:97483-97502 | None:intergenic | 45.0% |
| ! | TGGCTAAGTTTGGTGGATCT+TGG | - | contig246end:97449-97468 | MsG0080048224.01.T01:CDS | 45.0% |
| !! | GGTGATCGGTTGGTGATATT+CGG | - | contig246end:97580-97599 | MsG0080048224.01.T01:CDS | 45.0% |
| AAGAGTGAGAGCAGCATCGT+GGG | - | contig246end:97559-97578 | MsG0080048224.01.T01:CDS | 50.0% | |
| TCTTGGCACGCTCACTAGTA+AGG | + | contig246end:97627-97646 | None:intergenic | 50.0% | |
| TTCCAGCACCTTATGCGAGA+TGG | - | contig246end:97199-97218 | MsG0080048224.01.T01:CDS | 50.0% | |
| AGAGCAGCATCGTGGGTGAT+CGG | - | contig246end:97566-97585 | MsG0080048224.01.T01:CDS | 55.0% | |
| AGCGAACACCTTCTCCAGCT+GGG | - | contig246end:97517-97536 | MsG0080048224.01.T01:CDS | 55.0% | |
| CACCTTATGCGAGATGGCGA+AGG | - | contig246end:97205-97224 | MsG0080048224.01.T01:CDS | 55.0% | |
| GAAGAGTGAGAGCAGCATCG+TGG | - | contig246end:97558-97577 | MsG0080048224.01.T01:CDS | 55.0% | |
| GACCTTCGCCATCTCGCATA+AGG | + | contig246end:97210-97229 | None:intergenic | 55.0% | |
| TCCTGCGTTACCGCCTCAAA+TGG | - | contig246end:97647-97666 | MsG0080048224.01.T01:CDS | 55.0% | |
| TTCGGAGACATGCAGCAGAG+AGG | - | contig246end:97598-97617 | MsG0080048224.01.T01:CDS | 55.0% | |
| ! | ACCATTTGAGGCGGTAACGC+AGG | + | contig246end:97651-97670 | None:intergenic | 55.0% |
| ! | CATTTTCTCGATCCCCCAGC+TGG | + | contig246end:97534-97553 | None:intergenic | 55.0% |
| CAGCATCGTGGGTGATCGGT+TGG | - | contig246end:97570-97589 | MsG0080048224.01.T01:CDS | 60.0% | |
| CAGCGAACACCTTCTCCAGC+TGG | - | contig246end:97516-97535 | MsG0080048224.01.T01:CDS | 60.0% | |
| CGAACACCTTCTCCAGCTGG+GGG | - | contig246end:97519-97538 | MsG0080048224.01.T01:CDS | 60.0% | |
| CGCCATCTCGCATAAGGTGC+TGG | + | contig246end:97204-97223 | None:intergenic | 60.0% | |
| GAGGCGGTAACGCAGGATCT+TGG | + | contig246end:97644-97663 | None:intergenic | 60.0% | |
| GCGAACACCTTCTCCAGCTG+GGG | - | contig246end:97518-97537 | MsG0080048224.01.T01:CDS | 60.0% | |
| GGAGACATGCAGCAGAGAGG+TGG | - | contig246end:97601-97620 | MsG0080048224.01.T01:CDS | 60.0% | |
| CTCGATCCCCCAGCTGGAGA+AGG | + | contig246end:97528-97547 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig246end | gene | 97166 | 97777 | 97166 | ID=MsG0080048224.01;Name=MsG0080048224.01 |
| contig246end | mRNA | 97166 | 97777 | 97166 | ID=MsG0080048224.01.T01;Parent=MsG0080048224.01;Name=MsG0080048224.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|203 |
| contig246end | exon | 97166 | 97777 | 97166 | ID=MsG0080048224.01.T01:exon:36863;Parent=MsG0080048224.01.T01 |
| contig246end | CDS | 97166 | 97777 | 97166 | ID=MsG0080048224.01.T01:cds;Parent=MsG0080048224.01.T01 |
| Gene Sequence |
| Protein sequence |
