AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005048.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005048.01.T01 MTR_1g492910 100.000 137 0 0 1 137 1 137 2.39e-95 271
MsG0180005048.01.T01 MTR_1g090780 99.270 137 1 0 1 137 1 137 1.30e-94 269
MsG0180005048.01.T01 MTR_4g063240 86.395 147 10 3 1 137 1 147 7.38e-81 234
MsG0180005048.01.T01 MTR_4g063220 87.162 148 8 3 1 137 1 148 3.71e-77 225
MsG0180005048.01.T01 MTR_4g063990 87.162 148 8 3 1 137 1 148 3.71e-77 225
MsG0180005048.01.T01 MTR_4g063200 87.162 148 8 3 1 137 1 148 3.71e-77 225
MsG0180005048.01.T01 MTR_4g064020 87.162 148 8 3 1 137 1 148 3.71e-77 225
MsG0180005048.01.T01 MTR_4g071180 87.162 148 8 3 1 137 1 148 3.25e-76 223
MsG0180005048.01.T01 MTR_4g070240 87.162 148 8 3 1 137 1 148 3.25e-76 223
MsG0180005048.01.T01 MTR_4g088025 89.510 143 9 3 1 137 1 143 6.62e-76 222
MsG0180005048.01.T01 MTR_4g070245 85.811 148 10 3 1 137 1 148 1.51e-74 219
MsG0180005048.01.T01 MTR_4g063275 84.459 148 12 3 1 137 1 148 6.70e-74 217
MsG0180005048.01.T01 MTR_4g064983 86.111 144 9 3 1 133 1 144 1.59e-72 217
MsG0180005048.01.T01 MTR_7g099960 95.413 109 4 1 29 137 31 138 2.62e-64 192
MsG0180005048.01.T01 MTR_1g068600 93.578 109 5 1 29 137 30 136 3.09e-63 189
MsG0180005048.01.T01 MTR_2g084480 61.798 89 34 0 48 136 136 224 1.92e-37 127
MsG0180005048.01.T01 MTR_8g069990 69.663 89 24 1 8 96 3 88 7.98e-37 121
MsG0180005048.01.T01 MTR_4g063270 90.196 51 5 0 75 125 1 51 5.42e-27 95.5
MsG0180005048.01.T01 MTR_7g114580 90.244 41 4 0 97 137 11 51 6.05e-20 77.8
MsG0180005048.01.T01 MTR_4g032777 36.364 99 58 1 28 121 12 110 4.00e-13 62.4
MsG0180005048.01.T01 MTR_2g083800 67.273 55 15 2 8 61 11 63 1.79e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005048.01.T01 AT2G37470 88.406 138 14 2 1 136 1 138 5.10e-75 219
MsG0180005048.01.T01 AT3G45980 83.333 150 12 3 1 137 1 150 2.70e-74 218
MsG0180005048.01.T01 AT2G28720 82.119 151 13 2 1 137 1 151 4.33e-74 218
MsG0180005048.01.T01 AT3G53650 86.331 139 16 3 1 137 1 138 1.61e-71 211
MsG0180005048.01.T01 AT1G07790 83.333 150 10 4 1 137 1 148 1.96e-71 211
MsG0180005048.01.T01 AT5G59910 87.313 134 15 2 6 137 17 150 6.77e-70 207
MsG0180005048.01.T01 AT3G46030 89.147 129 12 2 11 137 17 145 2.34e-69 206
MsG0180005048.01.T01 AT5G22880 80.282 142 14 2 10 137 4 145 2.02e-66 198
MsG0180005048.01.T01 AT5G02570 84.173 139 13 2 1 137 1 132 3.08e-66 197
MsG0180005048.01.T01 AT3G09480 79.562 137 17 2 1 137 1 126 2.08e-62 187
MsG0180005048.01.T01 AT1G08170 56.989 93 40 0 42 134 143 235 7.05e-38 129

Find 32 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTCTTCTTCTTCTTAT+CGG 0.211855 1:-86177260 None:intergenic
GCTGTTCGTCTTGTTCTTCC+TGG 0.277831 1:+86177481 MsG0180005048.01.T01:CDS
CTTGAAGATGTAGATCTTGT+AGG 0.279657 1:-86177299 None:intergenic
CATTCAAGAGCTAGTGAATT+TGG 0.280274 1:-86177548 None:intergenic
TCGGTTGAGGATGCTTCCTT+TGG 0.316023 1:-86177241 None:intergenic
TCTGCAGGTGCTTTCTCTGC+AGG 0.340236 1:-86177193 None:intergenic
CGGTATCTCAAGCAAGGCTA+TGG 0.396652 1:+86177348 MsG0180005048.01.T01:CDS
CTTGTTGTAGCGAGCAAGTC+TGG 0.401541 1:-86177425 None:intergenic
GCGGGTTTCTTCTCTCCCTT+CGG 0.413410 1:-86177160 None:intergenic
CTTCTTCTTCTTATCGGTTG+AGG 0.416596 1:-86177254 None:intergenic
AGTGAATTTGGTGACAGCTT+TGG 0.420989 1:-86177536 None:intergenic
CTACAAGATCTACATCTTCA+AGG 0.431521 1:+86177300 MsG0180005048.01.T01:CDS
CGGTTGAGGATGCTTCCTTT+GGG 0.432726 1:-86177240 None:intergenic
TCCCTCGGAGACAGCGTGCT+TGG 0.447024 1:-86177512 None:intergenic
CATCTTCAAGGTTCTCAAGC+AGG 0.470454 1:+86177312 MsG0180005048.01.T01:CDS
TCTGCAGGCTTCTTCTCAGC+GGG 0.478601 1:-86177178 None:intergenic
GCGTGCTTGGCGAGTTCACC+AGG 0.485011 1:-86177499 None:intergenic
GGTGACAGCTTTGGTTCCCT+CGG 0.503972 1:-86177527 None:intergenic
CATCTTCGAGAAGCTCGCTC+AGG 0.521238 1:+86177396 MsG0180005048.01.T01:CDS
GGTATCTCAAGCAAGGCTAT+GGG 0.525343 1:+86177349 MsG0180005048.01.T01:CDS
CTCTGCAGGCTTCTTCTCAG+CGG 0.539158 1:-86177179 None:intergenic
TCGTTCAACAATGGCACCGA+AGG 0.539447 1:+86177144 None:intergenic
TTGCTTGAGATACCGATGTC+AGG 0.547522 1:-86177340 None:intergenic
CTGAATCTCACGAGAAGTGA+TGG 0.558881 1:-86177455 None:intergenic
GCCAAGCACGCTGTCTCCGA+GGG 0.566568 1:+86177511 MsG0180005048.01.T01:CDS
CGCCAAGCACGCTGTCTCCG+AGG 0.573257 1:+86177510 MsG0180005048.01.T01:CDS
CGTTCAACAATGGCACCGAA+GGG 0.602660 1:+86177145 None:intergenic
TGACATCGGTATCTCAAGCA+AGG 0.623015 1:+86177342 MsG0180005048.01.T01:CDS
AAGCAGGTTCATCCTGACAT+CGG 0.661631 1:+86177328 MsG0180005048.01.T01:CDS
AGCAGAGAAGAAGATCCCAA+AGG 0.665852 1:+86177225 MsG0180005048.01.T01:CDS
GTATCTCAAGCAAGGCTATG+GGG 0.674185 1:+86177350 MsG0180005048.01.T01:CDS
ATCTCACGAGAAGTGATGGT+AGG 0.676602 1:-86177451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTTCTCTTCTTCTTCTTAT+CGG - Chr1:86177263-86177282 None:intergenic 30.0%
CTACAAGATCTACATCTTCA+AGG + Chr1:86177300-86177319 MsG0180005048.01.T01:CDS 35.0%
CTTGAAGATGTAGATCTTGT+AGG - Chr1:86177302-86177321 None:intergenic 35.0%
! AGTGAATTTGGTGACAGCTT+TGG - Chr1:86177539-86177558 None:intergenic 40.0%
! CTTCTTCTTCTTATCGGTTG+AGG - Chr1:86177257-86177276 None:intergenic 40.0%
AAGCAGGTTCATCCTGACAT+CGG + Chr1:86177328-86177347 MsG0180005048.01.T01:CDS 45.0%
AGCAGAGAAGAAGATCCCAA+AGG + Chr1:86177225-86177244 MsG0180005048.01.T01:CDS 45.0%
ATCTCACGAGAAGTGATGGT+AGG - Chr1:86177454-86177473 None:intergenic 45.0%
CATCTTCAAGGTTCTCAAGC+AGG + Chr1:86177312-86177331 MsG0180005048.01.T01:CDS 45.0%
CTGAATCTCACGAGAAGTGA+TGG - Chr1:86177458-86177477 None:intergenic 45.0%
GGTATCTCAAGCAAGGCTAT+GGG + Chr1:86177349-86177368 MsG0180005048.01.T01:CDS 45.0%
GTATCTCAAGCAAGGCTATG+GGG + Chr1:86177350-86177369 MsG0180005048.01.T01:CDS 45.0%
TGACATCGGTATCTCAAGCA+AGG + Chr1:86177342-86177361 MsG0180005048.01.T01:CDS 45.0%
TTGCTTGAGATACCGATGTC+AGG - Chr1:86177343-86177362 None:intergenic 45.0%
! TCTGCTTTCGTTTTCTCTGC+AGG - Chr1:86177211-86177230 None:intergenic 45.0%
CGGTATCTCAAGCAAGGCTA+TGG + Chr1:86177348-86177367 MsG0180005048.01.T01:CDS 50.0%
CGGTTGAGGATGCTTCCTTT+GGG - Chr1:86177243-86177262 None:intergenic 50.0%
TCGGTTGAGGATGCTTCCTT+TGG - Chr1:86177244-86177263 None:intergenic 50.0%
! CTTGTTGTAGCGAGCAAGTC+TGG - Chr1:86177428-86177447 None:intergenic 50.0%
! GCTGTTCGTCTTGTTCTTCC+TGG + Chr1:86177481-86177500 MsG0180005048.01.T01:CDS 50.0%
CATCTTCGAGAAGCTCGCTC+AGG + Chr1:86177396-86177415 MsG0180005048.01.T01:CDS 55.0%
CTCTGCAGGCTTCTTCTCAG+CGG - Chr1:86177182-86177201 None:intergenic 55.0%
GCGGGTTTCTTCTCTCCCTT+CGG - Chr1:86177163-86177182 None:intergenic 55.0%
TCTGCAGGCTTCTTCTCAGC+GGG - Chr1:86177181-86177200 None:intergenic 55.0%
TCTGCAGGTGCTTTCTCTGC+AGG - Chr1:86177196-86177215 None:intergenic 55.0%
! GGTGACAGCTTTGGTTCCCT+CGG - Chr1:86177530-86177549 None:intergenic 55.0%
GCCAAGCACGCTGTCTCCGA+GGG + Chr1:86177511-86177530 MsG0180005048.01.T01:CDS 65.0%
GCGTGCTTGGCGAGTTCACC+AGG - Chr1:86177502-86177521 None:intergenic 65.0%
TCCCTCGGAGACAGCGTGCT+TGG - Chr1:86177515-86177534 None:intergenic 65.0%
CGCCAAGCACGCTGTCTCCG+AGG + Chr1:86177510-86177529 MsG0180005048.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 86177154 86177567 86177154 ID=MsG0180005048.01;Name=MsG0180005048.01
Chr1 mRNA 86177154 86177567 86177154 ID=MsG0180005048.01.T01;Parent=MsG0180005048.01;Name=MsG0180005048.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|137
Chr1 exon 86177154 86177567 86177154 ID=MsG0180005048.01.T01:exon:9890;Parent=MsG0180005048.01.T01
Chr1 CDS 86177154 86177567 86177154 ID=MsG0180005048.01.T01:cds;Parent=MsG0180005048.01.T01
Gene Sequence

>MsG0180005048.01.T01

ATGGCACCGAAGGGAGAGAAGAAACCCGCTGAGAAGAAGCCTGCAGAGAAAGCACCTGCAGAGAAAACGAAAGCAGAGAAGAAGATCCCAAAGGAAGCATCCTCAACCGATAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATCGGTATCTCAAGCAAGGCTATGGGGATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCTCAGGAATCTTCCAGACTTGCTCGCTACAACAAGAAACCTACCATCACTTCTCGTGAGATTCAGACTGCTGTTCGTCTTGTTCTTCCTGGTGAACTCGCCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCTCTTGA

Protein sequence

>MsG0180005048.01.T01

MAPKGEKKPAEKKPAEKAPAEKTKAEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*