AlfalfaGEDB | MsG0180005048.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005048.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005048.01.T01	XP_013469395.1	100	137	0	0	1	137	1	137	1.98E-91	271

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005048.01.T01	Q1S9I9	87.162	148	8	3	1	137	1	148	3.34E-75	223

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005048.01.T01	I3S6Z4	100.000	137	0	0	1	137	1	137	9.44e-92	271

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0180005048.01	0.841334	5.061156e-58	7.412008e-55
MsG0080048258.01	MsG0180005048.01	0.840991	6.229962e-58	9.027256e-55
MsG0080048775.01	MsG0180005048.01	0.866369	2.997608e-65	1.007832e-61
MsG0080048781.01	MsG0180005048.01	0.863083	3.206937e-64	9.603400e-61
MsG0180000718.01	MsG0180005048.01	0.829348	5.414664e-55	5.527855e-52
MsG0180001293.01	MsG0180005048.01	0.863904	1.784520e-64	5.498792e-61
MsG0180001719.01	MsG0180005048.01	0.827816	1.269186e-54	1.238751e-51
MsG0180002134.01	MsG0180005048.01	0.830291	3.190806e-55	3.347367e-52
MsG0180002218.01	MsG0180005048.01	0.895375	1.017859e-75	1.053193e-71
MsG0180002397.01	MsG0180005048.01	0.895054	1.378712e-75	1.407463e-71

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005048.01.T01	MTR_1g492910	100.000	137	0	0	1	137	1	137	2.39e-95	271
MsG0180005048.01.T01	MTR_1g090780	99.270	137	1	0	1	137	1	137	1.30e-94	269
MsG0180005048.01.T01	MTR_4g063240	86.395	147	10	3	1	137	1	147	7.38e-81	234
MsG0180005048.01.T01	MTR_4g063220	87.162	148	8	3	1	137	1	148	3.71e-77	225
MsG0180005048.01.T01	MTR_4g063990	87.162	148	8	3	1	137	1	148	3.71e-77	225
MsG0180005048.01.T01	MTR_4g063200	87.162	148	8	3	1	137	1	148	3.71e-77	225
MsG0180005048.01.T01	MTR_4g064020	87.162	148	8	3	1	137	1	148	3.71e-77	225
MsG0180005048.01.T01	MTR_4g071180	87.162	148	8	3	1	137	1	148	3.25e-76	223
MsG0180005048.01.T01	MTR_4g070240	87.162	148	8	3	1	137	1	148	3.25e-76	223
MsG0180005048.01.T01	MTR_4g088025	89.510	143	9	3	1	137	1	143	6.62e-76	222

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005048.01.T01	AT2G37470	88.406	138	14	2	1	136	1	138	5.10e-75	219
MsG0180005048.01.T01	AT3G45980	83.333	150	12	3	1	137	1	150	2.70e-74	218
MsG0180005048.01.T01	AT2G28720	82.119	151	13	2	1	137	1	151	4.33e-74	218
MsG0180005048.01.T01	AT3G53650	86.331	139	16	3	1	137	1	138	1.61e-71	211
MsG0180005048.01.T01	AT1G07790	83.333	150	10	4	1	137	1	148	1.96e-71	211
MsG0180005048.01.T01	AT5G59910	87.313	134	15	2	6	137	17	150	6.77e-70	207
MsG0180005048.01.T01	AT3G46030	89.147	129	12	2	11	137	17	145	2.34e-69	206
MsG0180005048.01.T01	AT5G22880	80.282	142	14	2	10	137	4	145	2.02e-66	198
MsG0180005048.01.T01	AT5G02570	84.173	139	13	2	1	137	1	132	3.08e-66	197
MsG0180005048.01.T01	AT3G09480	79.562	137	17	2	1	137	1	126	2.08e-62	187

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Find 32 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCAGGTTCATCCTGACAT+CGG	0.661631	1:+86177328	MsG0180005048.01.T01:CDS
AGCAGAGAAGAAGATCCCAA+AGG	0.665852	1:+86177225	MsG0180005048.01.T01:CDS
AGTGAATTTGGTGACAGCTT+TGG	0.420989	1:-86177536	None:intergenic
ATCTCACGAGAAGTGATGGT+AGG	0.676602	1:-86177451	None:intergenic
CATCTTCAAGGTTCTCAAGC+AGG	0.470454	1:+86177312	MsG0180005048.01.T01:CDS
CATCTTCGAGAAGCTCGCTC+AGG	0.521238	1:+86177396	MsG0180005048.01.T01:CDS
CATTCAAGAGCTAGTGAATT+TGG	0.280274	1:-86177548	None:intergenic
CGCCAAGCACGCTGTCTCCG+AGG	0.573257	1:+86177510	MsG0180005048.01.T01:CDS
CGGTATCTCAAGCAAGGCTA+TGG	0.396652	1:+86177348	MsG0180005048.01.T01:CDS
CGGTTGAGGATGCTTCCTTT+GGG	0.432726	1:-86177240	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTTCTCTTCTTCTTCTTAT+CGG	-	Chr1:86177263-86177282	None:intergenic	30.0%
CTACAAGATCTACATCTTCA+AGG	+	Chr1:86177300-86177319	MsG0180005048.01.T01:CDS	35.0%
CTTGAAGATGTAGATCTTGT+AGG	-	Chr1:86177302-86177321	None:intergenic	35.0%
AAGCAGGTTCATCCTGACAT+CGG	+	Chr1:86177328-86177347	MsG0180005048.01.T01:CDS	45.0%
AGCAGAGAAGAAGATCCCAA+AGG	+	Chr1:86177225-86177244	MsG0180005048.01.T01:CDS	45.0%
ATCTCACGAGAAGTGATGGT+AGG	-	Chr1:86177454-86177473	None:intergenic	45.0%
CATCTTCAAGGTTCTCAAGC+AGG	+	Chr1:86177312-86177331	MsG0180005048.01.T01:CDS	45.0%
CTGAATCTCACGAGAAGTGA+TGG	-	Chr1:86177458-86177477	None:intergenic	45.0%
GGTATCTCAAGCAAGGCTAT+GGG	+	Chr1:86177349-86177368	MsG0180005048.01.T01:CDS	45.0%
GTATCTCAAGCAAGGCTATG+GGG	+	Chr1:86177350-86177369	MsG0180005048.01.T01:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	86177154	86177567	86177154	ID=MsG0180005048.01;Name=MsG0180005048.01
Chr1	mRNA	86177154	86177567	86177154	ID=MsG0180005048.01.T01;Parent=MsG0180005048.01;Name=MsG0180005048.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|137
Chr1	exon	86177154	86177567	86177154	ID=MsG0180005048.01.T01:exon:9890;Parent=MsG0180005048.01.T01
Chr1	CDS	86177154	86177567	86177154	ID=MsG0180005048.01.T01:cds;Parent=MsG0180005048.01.T01

Gene Sequence

>MsG0180005048.01.T01

ATGGCACCGAAGGGAGAGAAGAAACCCGCTGAGAAGAAGCCTGCAGAGAAAGCACCTGCAGAGAAAACGAAAGCAGAGAAGAAGATCCCAAAGGAAGCATCCTCAACCGATAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATCGGTATCTCAAGCAAGGCTATGGGGATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCTCAGGAATCTTCCAGACTTGCTCGCTACAACAAGAAACCTACCATCACTTCTCGTGAGATTCAGACTGCTGTTCGTCTTGTTCTTCCTGGTGAACTCGCCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCTCTTGA

Protein sequence

>MsG0180005048.01.T01

MAPKGEKKPAEKKPAEKAPAEKTKAEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*