Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005564.01.T01 | AAL84187.1 | 87.931 | 174 | 19 | 1 | 46 | 217 | 1 | 174 | 1.83E-102 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005564.01.T01 | Q8S4Q1 | 87.931 | 174 | 19 | 1 | 46 | 217 | 1 | 174 | 8.75e-103 | 303 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002770.01 | MsG0180005564.01 | 0.865497 | 5.659860e-65 | 1.845194e-61 |
| MsG0180005564.01 | MsG0480018276.01 | 0.800311 | 1.529772e-48 | 7.100460e-46 |
| MsG0180005564.01 | MsG0480018277.01 | 0.845130 | 4.919416e-59 | 8.116676e-56 |
| MsG0180005564.01 | MsG0680035744.01 | 0.828303 | 9.692498e-55 | 9.595579e-52 |
| MsG0180005564.01 | MsG0780038885.01 | 0.899329 | 2.206910e-77 | 2.716540e-73 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005564.01.T01 | MTR_3g030260 | 67.249 | 229 | 63 | 3 | 1 | 217 | 1 | 229 | 6.46e-92 | 269 |
| MsG0180005564.01.T01 | MTR_6g033455 | 61.905 | 231 | 74 | 4 | 1 | 217 | 1 | 231 | 1.29e-86 | 256 |
| MsG0180005564.01.T01 | MTR_2g009950 | 65.198 | 227 | 67 | 3 | 1 | 215 | 1 | 227 | 1.57e-85 | 255 |
| MsG0180005564.01.T01 | MTR_0510s0010 | 58.009 | 231 | 83 | 4 | 1 | 217 | 8 | 238 | 6.44e-77 | 231 |
| MsG0180005564.01.T01 | MTR_7g016020 | 63.212 | 193 | 59 | 3 | 1 | 181 | 1 | 193 | 3.48e-59 | 190 |
| MsG0180005564.01.T01 | MTR_2g083020 | 63.731 | 193 | 58 | 4 | 1 | 181 | 1 | 193 | 2.09e-56 | 191 |
| MsG0180005564.01.T01 | MTR_7g045350 | 62.774 | 137 | 39 | 3 | 92 | 216 | 23 | 159 | 2.30e-42 | 140 |
| MsG0180005564.01.T01 | MTR_0053s0080 | 52.632 | 190 | 77 | 3 | 1 | 177 | 1 | 190 | 7.30e-35 | 127 |
| MsG0180005564.01.T01 | MTR_8g461570 | 77.027 | 74 | 16 | 1 | 145 | 217 | 5 | 78 | 1.98e-32 | 112 |
| MsG0180005564.01.T01 | MTR_0012s0290 | 64.228 | 123 | 32 | 3 | 92 | 202 | 1147 | 1269 | 3.26e-32 | 123 |
| MsG0180005564.01.T01 | MTR_7g074720 | 79.452 | 73 | 14 | 1 | 146 | 217 | 2 | 74 | 2.20e-31 | 110 |
| MsG0180005564.01.T01 | MTR_5g454260 | 63.793 | 116 | 42 | 0 | 1 | 116 | 1 | 116 | 1.03e-30 | 113 |
| MsG0180005564.01.T01 | MTR_3g026190 | 48.598 | 107 | 50 | 2 | 66 | 167 | 825 | 931 | 3.11e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGACTCTCTCAAACATATT+TGG | 0.156108 | 1:-93435466 | None:intergenic |
| GTGATAAATTCTTCAATCTT+TGG | 0.234620 | 1:-93435363 | None:intergenic |
| GGAGGAATTCCATGAACTTT+AGG | 0.268489 | 1:-93435780 | None:intergenic |
| ATGTGTTGTCCGTCTCATTT+TGG | 0.313383 | 1:+93435827 | MsG0180005564.01.T01:CDS |
| TCCTTTGAAACCCTACGCTT+TGG | 0.349397 | 1:-93435802 | None:intergenic |
| GCCAACAATCAATCCATTCT+TGG | 0.351499 | 1:+93435427 | MsG0180005564.01.T01:CDS |
| CCTTTGAAACCCTACGCTTT+GGG | 0.365038 | 1:-93435801 | None:intergenic |
| GGTATCTTGCATGTTTATCA+TGG | 0.380452 | 1:-93435338 | None:intergenic |
| AGAAGTGATAGAGGTGGTAG+AGG | 0.393182 | 1:+93435618 | MsG0180005564.01.T01:CDS |
| GGAAGAAACATTTGAACAAA+AGG | 0.395964 | 1:+93435657 | MsG0180005564.01.T01:CDS |
| ACATTGTTGATATTGTTGAT+AGG | 0.404560 | 1:-93435254 | None:intergenic |
| TGAGCAAGACTGAAATTTAC+CGG | 0.405679 | 1:+93435689 | MsG0180005564.01.T01:CDS |
| TCCAAGAATGGATTGATTGT+TGG | 0.413744 | 1:-93435428 | None:intergenic |
| GTCCATGACCTCCTGAACTT+TGG | 0.414042 | 1:-93435742 | None:intergenic |
| TCAGCTGTACCTAAAGTTCA+TGG | 0.424076 | 1:+93435771 | MsG0180005564.01.T01:CDS |
| TTATGAAGATGAGAGATATT+CGG | 0.429317 | 1:+93435279 | MsG0180005564.01.T01:CDS |
| GTTGATTCAAGCACAAATCA+TGG | 0.442225 | 1:+93435396 | MsG0180005564.01.T01:CDS |
| CCTTGTTCAAACCAAAGTTC+AGG | 0.445067 | 1:+93435731 | MsG0180005564.01.T01:CDS |
| GAATGGATTGATTGTTGGCT+TGG | 0.457063 | 1:-93435423 | None:intergenic |
| GTGGCCAACACTGTTGATAA+TGG | 0.457450 | 1:+93435595 | MsG0180005564.01.T01:CDS |
| TAAATTCTTCAATCTTTGGC+TGG | 0.462835 | 1:-93435359 | None:intergenic |
| TTGTTGATAGGATTCATACT+TGG | 0.469352 | 1:-93435242 | None:intergenic |
| GAAGAAACATTTGAACAAAA+GGG | 0.474308 | 1:+93435658 | MsG0180005564.01.T01:CDS |
| GACAAAATCATGGCTGATCA+TGG | 0.485385 | 1:+93435196 | None:intergenic |
| AGAAGTTGTTGTTCAAAATG+AGG | 0.492337 | 1:+93435501 | MsG0180005564.01.T01:CDS |
| CCTGAACTTTGGTTTGAACA+AGG | 0.502302 | 1:-93435731 | None:intergenic |
| CGCAAATTTCATCAATGACC+CGG | 0.514753 | 1:-93435708 | None:intergenic |
| TGTTGATAGGATTCATACTT+GGG | 0.517284 | 1:-93435241 | None:intergenic |
| AACTTTAGGTACAGCTGATG+TGG | 0.527312 | 1:-93435766 | None:intergenic |
| TTCTCCATTATCAACAGTGT+TGG | 0.531055 | 1:-93435599 | None:intergenic |
| AAGACGAACTTCCTCAAGAA+AGG | 0.539436 | 1:+93435560 | MsG0180005564.01.T01:CDS |
| AACCAAAGTTCAGGAGGTCA+TGG | 0.540463 | 1:+93435740 | MsG0180005564.01.T01:CDS |
| TGGAATTCCTCCCAAAGCGT+AGG | 0.545471 | 1:+93435791 | MsG0180005564.01.T01:CDS |
| CCCAAAGCGTAGGGTTTCAA+AGG | 0.548667 | 1:+93435801 | MsG0180005564.01.T01:CDS |
| TCAATATTTGACCTTTCTTG+AGG | 0.555926 | 1:-93435571 | None:intergenic |
| CCAAATATGTTTGAGAGAGT+CGG | 0.559778 | 1:+93435466 | MsG0180005564.01.T01:CDS |
| GGAATTCCTCCCAAAGCGTA+GGG | 0.566799 | 1:+93435792 | MsG0180005564.01.T01:CDS |
| TGATAATGGAGAAGTGATAG+AGG | 0.583165 | 1:+93435609 | MsG0180005564.01.T01:CDS |
| AGAAAGGTCAAATATTGAAG+TGG | 0.590226 | 1:+93435576 | MsG0180005564.01.T01:CDS |
| AAGAAACATTTGAACAAAAG+GGG | 0.596132 | 1:+93435659 | MsG0180005564.01.T01:CDS |
| TGATAGGATTCATACTTGGG+TGG | 0.604185 | 1:-93435238 | None:intergenic |
| TTGAAACCCTACGCTTTGGG+AGG | 0.607057 | 1:-93435798 | None:intergenic |
| AGAGGAACGATTACTTAGCA+AGG | 0.614620 | 1:+93435636 | MsG0180005564.01.T01:CDS |
| GTTACAACATTAGAAGAATG+TGG | 0.625446 | 1:+93435526 | MsG0180005564.01.T01:CDS |
| TAATGGAGAAGTGATAGAGG+TGG | 0.631937 | 1:+93435612 | MsG0180005564.01.T01:CDS |
| TGAGGAAGTTCGTCTTTGTG+TGG | 0.633527 | 1:-93435553 | None:intergenic |
| TGTTCAAACCAAAGTTCAGG+AGG | 0.642083 | 1:+93435734 | MsG0180005564.01.T01:CDS |
| GAGCAAGACTGAAATTTACC+GGG | 0.661857 | 1:+93435690 | MsG0180005564.01.T01:CDS |
| TTAGGGTGGCCAAAATGAGA+CGG | 0.662957 | 1:-93435836 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAACATTTGAACAAAAG+GGG | + | Chr1:93435659-93435678 | MsG0180005564.01.T01:CDS | 25.0% |
| ! | ACATTGTTGATATTGTTGAT+AGG | - | Chr1:93435257-93435276 | None:intergenic | 25.0% |
| ! | GAAGAAACATTTGAACAAAA+GGG | + | Chr1:93435658-93435677 | MsG0180005564.01.T01:CDS | 25.0% |
| ! | GTGATAAATTCTTCAATCTT+TGG | - | Chr1:93435366-93435385 | None:intergenic | 25.0% |
| ! | TTATGAAGATGAGAGATATT+CGG | + | Chr1:93435279-93435298 | MsG0180005564.01.T01:CDS | 25.0% |
| AGAAAGGTCAAATATTGAAG+TGG | + | Chr1:93435576-93435595 | MsG0180005564.01.T01:CDS | 30.0% | |
| GGAAGAAACATTTGAACAAA+AGG | + | Chr1:93435657-93435676 | MsG0180005564.01.T01:CDS | 30.0% | |
| GTTACAACATTAGAAGAATG+TGG | + | Chr1:93435526-93435545 | MsG0180005564.01.T01:CDS | 30.0% | |
| TAAATTCTTCAATCTTTGGC+TGG | - | Chr1:93435362-93435381 | None:intergenic | 30.0% | |
| TCAATATTTGACCTTTCTTG+AGG | - | Chr1:93435574-93435593 | None:intergenic | 30.0% | |
| ! | AGAAGTTGTTGTTCAAAATG+AGG | + | Chr1:93435501-93435520 | MsG0180005564.01.T01:CDS | 30.0% |
| ! | ATTTTCTTGTTCTCCAAGAA+TGG | - | Chr1:93435443-93435462 | None:intergenic | 30.0% |
| ! | TGTTGATAGGATTCATACTT+GGG | - | Chr1:93435244-93435263 | None:intergenic | 30.0% |
| ! | TTGTTGATAGGATTCATACT+TGG | - | Chr1:93435245-93435264 | None:intergenic | 30.0% |
| CCAAATATGTTTGAGAGAGT+CGG | + | Chr1:93435466-93435485 | MsG0180005564.01.T01:CDS | 35.0% | |
| GGTATCTTGCATGTTTATCA+TGG | - | Chr1:93435341-93435360 | None:intergenic | 35.0% | |
| TGAGCAAGACTGAAATTTAC+CGG | + | Chr1:93435689-93435708 | MsG0180005564.01.T01:CDS | 35.0% | |
| TTCTCCATTATCAACAGTGT+TGG | - | Chr1:93435602-93435621 | None:intergenic | 35.0% | |
| ! | GTTGATTCAAGCACAAATCA+TGG | + | Chr1:93435396-93435415 | MsG0180005564.01.T01:CDS | 35.0% |
| !! | TCCAAGAATGGATTGATTGT+TGG | - | Chr1:93435431-93435450 | None:intergenic | 35.0% |
| !! | TGATAATGGAGAAGTGATAG+AGG | + | Chr1:93435609-93435628 | MsG0180005564.01.T01:CDS | 35.0% |
| AACTTTAGGTACAGCTGATG+TGG | - | Chr1:93435769-93435788 | None:intergenic | 40.0% | |
| AAGACGAACTTCCTCAAGAA+AGG | + | Chr1:93435560-93435579 | MsG0180005564.01.T01:CDS | 40.0% | |
| AGAGGAACGATTACTTAGCA+AGG | + | Chr1:93435636-93435655 | MsG0180005564.01.T01:CDS | 40.0% | |
| CCGACTCTCTCAAACATATT+TGG | - | Chr1:93435469-93435488 | None:intergenic | 40.0% | |
| CCTTGTTCAAACCAAAGTTC+AGG | + | Chr1:93435731-93435750 | MsG0180005564.01.T01:CDS | 40.0% | |
| CGCAAATTTCATCAATGACC+CGG | - | Chr1:93435711-93435730 | None:intergenic | 40.0% | |
| GAGCAAGACTGAAATTTACC+GGG | + | Chr1:93435690-93435709 | MsG0180005564.01.T01:CDS | 40.0% | |
| GCCAACAATCAATCCATTCT+TGG | + | Chr1:93435427-93435446 | MsG0180005564.01.T01:CDS | 40.0% | |
| GGAGGAATTCCATGAACTTT+AGG | - | Chr1:93435783-93435802 | None:intergenic | 40.0% | |
| TAATGGAGAAGTGATAGAGG+TGG | + | Chr1:93435612-93435631 | MsG0180005564.01.T01:CDS | 40.0% | |
| TCAGCTGTACCTAAAGTTCA+TGG | + | Chr1:93435771-93435790 | MsG0180005564.01.T01:CDS | 40.0% | |
| TGATAGGATTCATACTTGGG+TGG | - | Chr1:93435241-93435260 | None:intergenic | 40.0% | |
| TGTTCAAACCAAAGTTCAGG+AGG | + | Chr1:93435734-93435753 | MsG0180005564.01.T01:CDS | 40.0% | |
| ! | ATGTGTTGTCCGTCTCATTT+TGG | + | Chr1:93435827-93435846 | MsG0180005564.01.T01:CDS | 40.0% |
| !! | CCTGAACTTTGGTTTGAACA+AGG | - | Chr1:93435734-93435753 | None:intergenic | 40.0% |
| !! | GAATGGATTGATTGTTGGCT+TGG | - | Chr1:93435426-93435445 | None:intergenic | 40.0% |
| AACCAAAGTTCAGGAGGTCA+TGG | + | Chr1:93435740-93435759 | MsG0180005564.01.T01:CDS | 45.0% | |
| AGAAGTGATAGAGGTGGTAG+AGG | + | Chr1:93435618-93435637 | MsG0180005564.01.T01:CDS | 45.0% | |
| TGAGGAAGTTCGTCTTTGTG+TGG | - | Chr1:93435556-93435575 | None:intergenic | 45.0% | |
| TTAGGGTGGCCAAAATGAGA+CGG | - | Chr1:93435839-93435858 | None:intergenic | 45.0% | |
| ! | GTGGCCAACACTGTTGATAA+TGG | + | Chr1:93435595-93435614 | MsG0180005564.01.T01:CDS | 45.0% |
| !! | CCTTTGAAACCCTACGCTTT+GGG | - | Chr1:93435804-93435823 | None:intergenic | 45.0% |
| !! | TCCTTTGAAACCCTACGCTT+TGG | - | Chr1:93435805-93435824 | None:intergenic | 45.0% |
| CCCAAAGCGTAGGGTTTCAA+AGG | + | Chr1:93435801-93435820 | MsG0180005564.01.T01:CDS | 50.0% | |
| GGAATTCCTCCCAAAGCGTA+GGG | + | Chr1:93435792-93435811 | MsG0180005564.01.T01:CDS | 50.0% | |
| GTCCATGACCTCCTGAACTT+TGG | - | Chr1:93435745-93435764 | None:intergenic | 50.0% | |
| TGGAATTCCTCCCAAAGCGT+AGG | + | Chr1:93435791-93435810 | MsG0180005564.01.T01:CDS | 50.0% | |
| ! | TTGAAACCCTACGCTTTGGG+AGG | - | Chr1:93435801-93435820 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 93435205 | 93435858 | 93435205 | ID=MsG0180005564.01;Name=MsG0180005564.01 |
| Chr1 | mRNA | 93435205 | 93435858 | 93435205 | ID=MsG0180005564.01.T01;Parent=MsG0180005564.01;Name=MsG0180005564.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|217 |
| Chr1 | exon | 93435205 | 93435858 | 93435205 | ID=MsG0180005564.01.T01:exon:16092;Parent=MsG0180005564.01.T01 |
| Chr1 | CDS | 93435205 | 93435858 | 93435205 | ID=MsG0180005564.01.T01:cds;Parent=MsG0180005564.01.T01 |
| Gene Sequence |
| Protein sequence |
