Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038885.01.T01 | AAL84187.1 | 88.506 | 174 | 18 | 1 | 46 | 217 | 1 | 174 | 3.74E-103 | 305 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038885.01.T01 | Q8S4Q1 | 88.506 | 174 | 18 | 1 | 46 | 217 | 1 | 174 | 1.79e-103 | 305 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002770.01 | MsG0780038885.01 | 0.917434 | 5.333899e-86 | 1.539301e-81 |
| MsG0180005564.01 | MsG0780038885.01 | 0.899329 | 2.206910e-77 | 2.716540e-73 |
| MsG0480018276.01 | MsG0780038885.01 | 0.854924 | 8.900449e-62 | 2.019987e-58 |
| MsG0480018277.01 | MsG0780038885.01 | 0.899906 | 1.245787e-77 | 1.572127e-73 |
| MsG0580028264.01 | MsG0780038885.01 | 0.842577 | 2.375999e-58 | 3.618176e-55 |
| MsG0280008929.01 | MsG0780038885.01 | 0.801055 | 1.078492e-48 | 5.101324e-46 |
| MsG0680035744.01 | MsG0780038885.01 | 0.880296 | 6.169073e-70 | 3.456269e-66 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038885.01.T01 | MTR_3g030260 | 67.686 | 229 | 62 | 3 | 1 | 217 | 1 | 229 | 9.85e-93 | 271 |
| MsG0780038885.01.T01 | MTR_6g033455 | 61.905 | 231 | 74 | 4 | 1 | 217 | 1 | 231 | 9.90e-87 | 256 |
| MsG0780038885.01.T01 | MTR_2g009950 | 65.639 | 227 | 66 | 3 | 1 | 215 | 1 | 227 | 2.74e-86 | 257 |
| MsG0780038885.01.T01 | MTR_0510s0010 | 58.009 | 231 | 83 | 4 | 1 | 217 | 8 | 238 | 5.06e-77 | 231 |
| MsG0780038885.01.T01 | MTR_7g016020 | 63.731 | 193 | 58 | 3 | 1 | 181 | 1 | 193 | 6.55e-60 | 192 |
| MsG0780038885.01.T01 | MTR_2g083020 | 64.249 | 193 | 57 | 4 | 1 | 181 | 1 | 193 | 4.66e-57 | 193 |
| MsG0780038885.01.T01 | MTR_7g045350 | 62.774 | 137 | 39 | 3 | 92 | 216 | 23 | 159 | 2.35e-42 | 140 |
| MsG0780038885.01.T01 | MTR_0053s0080 | 52.632 | 190 | 77 | 3 | 1 | 177 | 1 | 190 | 6.44e-35 | 127 |
| MsG0780038885.01.T01 | MTR_8g461570 | 78.378 | 74 | 15 | 1 | 145 | 217 | 5 | 78 | 3.98e-33 | 114 |
| MsG0780038885.01.T01 | MTR_0012s0290 | 64.228 | 123 | 32 | 3 | 92 | 202 | 1147 | 1269 | 2.85e-32 | 123 |
| MsG0780038885.01.T01 | MTR_7g074720 | 80.822 | 73 | 13 | 1 | 146 | 217 | 2 | 74 | 5.05e-32 | 111 |
| MsG0780038885.01.T01 | MTR_5g454260 | 63.793 | 116 | 42 | 0 | 1 | 116 | 1 | 116 | 1.01e-30 | 113 |
| MsG0780038885.01.T01 | MTR_3g026190 | 48.598 | 107 | 50 | 2 | 66 | 167 | 825 | 931 | 2.91e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGACTCTCTCAAACATATT+TGG | 0.156108 | 7:-54649083 | None:intergenic |
| GTGATAAATTCTTCAATCTT+TGG | 0.234620 | 7:-54648980 | None:intergenic |
| GGAGGAATTCCATGAACTTT+AGG | 0.268489 | 7:-54649397 | None:intergenic |
| ATGTGTTGTCCGTCTCATTT+TGG | 0.313383 | 7:+54649444 | MsG0780038885.01.T01:CDS |
| TCCTTTGAAACCCTACGCTT+TGG | 0.349397 | 7:-54649419 | None:intergenic |
| GCCAACAATCAATCCATTCT+TGG | 0.351499 | 7:+54649044 | MsG0780038885.01.T01:CDS |
| CCTTTGAAACCCTACGCTTT+GGG | 0.365038 | 7:-54649418 | None:intergenic |
| GGTATCTTGCATGTTTATCA+TGG | 0.380452 | 7:-54648955 | None:intergenic |
| AGAAGTGATAGAGGTGGTAG+AGG | 0.393182 | 7:+54649235 | MsG0780038885.01.T01:CDS |
| GGAAGAAACATTTGAACAAA+AGG | 0.395964 | 7:+54649274 | MsG0780038885.01.T01:CDS |
| TGAGCAAGGCTGAAATTTAC+CGG | 0.398275 | 7:+54649306 | MsG0780038885.01.T01:CDS |
| ACATTGTTGATATTGTTGAT+AGG | 0.404560 | 7:-54648871 | None:intergenic |
| TCCAAGAATGGATTGATTGT+TGG | 0.413744 | 7:-54649045 | None:intergenic |
| GTCCATGACCTCCTGAACTT+TGG | 0.414042 | 7:-54649359 | None:intergenic |
| TCAGCTGTACCTAAAGTTCA+TGG | 0.424076 | 7:+54649388 | MsG0780038885.01.T01:CDS |
| TTATGAAGATGAGAGATATT+CGG | 0.429317 | 7:+54648896 | MsG0780038885.01.T01:CDS |
| GTTGATTCAAGCACAAATCA+TGG | 0.442225 | 7:+54649013 | MsG0780038885.01.T01:CDS |
| CCTTGTTCAAACCAAAGTTC+AGG | 0.445067 | 7:+54649348 | MsG0780038885.01.T01:CDS |
| GAATGGATTGATTGTTGGCT+TGG | 0.457063 | 7:-54649040 | None:intergenic |
| GTGGCCAACACTGTTGATAA+TGG | 0.457450 | 7:+54649212 | MsG0780038885.01.T01:CDS |
| TAAATTCTTCAATCTTTGGC+TGG | 0.462835 | 7:-54648976 | None:intergenic |
| TTGTTGATAGGATTCATACT+TGG | 0.469352 | 7:-54648859 | None:intergenic |
| GAAGAAACATTTGAACAAAA+GGG | 0.474308 | 7:+54649275 | MsG0780038885.01.T01:CDS |
| GACAAAATCATGGCTGATCA+TGG | 0.485385 | 7:+54648813 | None:intergenic |
| AGAAGTTGTTGTTCAAAATG+AGG | 0.492337 | 7:+54649118 | MsG0780038885.01.T01:CDS |
| CCTGAACTTTGGTTTGAACA+AGG | 0.502302 | 7:-54649348 | None:intergenic |
| CGCAAATTTCATCAATGACC+CGG | 0.514753 | 7:-54649325 | None:intergenic |
| TGTTGATAGGATTCATACTT+GGG | 0.517284 | 7:-54648858 | None:intergenic |
| AACTTTAGGTACAGCTGATG+TGG | 0.527312 | 7:-54649383 | None:intergenic |
| TTCTCCATTATCAACAGTGT+TGG | 0.531055 | 7:-54649216 | None:intergenic |
| AAGACGAACTTCCTCAAGAA+AGG | 0.539436 | 7:+54649177 | MsG0780038885.01.T01:CDS |
| AACCAAAGTTCAGGAGGTCA+TGG | 0.540463 | 7:+54649357 | MsG0780038885.01.T01:CDS |
| TGGAATTCCTCCCAAAGCGT+AGG | 0.545471 | 7:+54649408 | MsG0780038885.01.T01:CDS |
| CCCAAAGCGTAGGGTTTCAA+AGG | 0.548667 | 7:+54649418 | MsG0780038885.01.T01:CDS |
| TCAATATTTGACCTTTCTTG+AGG | 0.555926 | 7:-54649188 | None:intergenic |
| CCAAATATGTTTGAGAGAGT+CGG | 0.559778 | 7:+54649083 | MsG0780038885.01.T01:CDS |
| GGAATTCCTCCCAAAGCGTA+GGG | 0.566799 | 7:+54649409 | MsG0780038885.01.T01:CDS |
| TGATAATGGAGAAGTGATAG+AGG | 0.583165 | 7:+54649226 | MsG0780038885.01.T01:CDS |
| AGAAAGGTCAAATATTGAAG+TGG | 0.590226 | 7:+54649193 | MsG0780038885.01.T01:CDS |
| AGAGGAACGGTTACTTAGCA+AGG | 0.590261 | 7:+54649253 | MsG0780038885.01.T01:CDS |
| AAGAAACATTTGAACAAAAG+GGG | 0.596132 | 7:+54649276 | MsG0780038885.01.T01:CDS |
| TGATAGGATTCATACTTGGG+TGG | 0.604185 | 7:-54648855 | None:intergenic |
| TTGAAACCCTACGCTTTGGG+AGG | 0.607057 | 7:-54649415 | None:intergenic |
| TGATAGAGGTGGTAGAGGAA+CGG | 0.612805 | 7:+54649240 | MsG0780038885.01.T01:CDS |
| GTTACAACATTAGAAGAATG+TGG | 0.625446 | 7:+54649143 | MsG0780038885.01.T01:CDS |
| TAATGGAGAAGTGATAGAGG+TGG | 0.631937 | 7:+54649229 | MsG0780038885.01.T01:CDS |
| TGAGGAAGTTCGTCTTTGTG+TGG | 0.633527 | 7:-54649170 | None:intergenic |
| AAAGGGGAAGAAAGTGAGCA+AGG | 0.636089 | 7:+54649292 | MsG0780038885.01.T01:CDS |
| TGTTCAAACCAAAGTTCAGG+AGG | 0.642083 | 7:+54649351 | MsG0780038885.01.T01:CDS |
| GAGCAAGGCTGAAATTTACC+GGG | 0.643159 | 7:+54649307 | MsG0780038885.01.T01:CDS |
| TTAGGGTGGCCAAAATGAGA+CGG | 0.662957 | 7:-54649453 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAACATTTGAACAAAAG+GGG | + | Chr7:54649276-54649295 | MsG0780038885.01.T01:CDS | 25.0% |
| ! | ACATTGTTGATATTGTTGAT+AGG | - | Chr7:54648874-54648893 | None:intergenic | 25.0% |
| ! | GAAGAAACATTTGAACAAAA+GGG | + | Chr7:54649275-54649294 | MsG0780038885.01.T01:CDS | 25.0% |
| ! | GTGATAAATTCTTCAATCTT+TGG | - | Chr7:54648983-54649002 | None:intergenic | 25.0% |
| ! | TTATGAAGATGAGAGATATT+CGG | + | Chr7:54648896-54648915 | MsG0780038885.01.T01:CDS | 25.0% |
| AGAAAGGTCAAATATTGAAG+TGG | + | Chr7:54649193-54649212 | MsG0780038885.01.T01:CDS | 30.0% | |
| GGAAGAAACATTTGAACAAA+AGG | + | Chr7:54649274-54649293 | MsG0780038885.01.T01:CDS | 30.0% | |
| GTTACAACATTAGAAGAATG+TGG | + | Chr7:54649143-54649162 | MsG0780038885.01.T01:CDS | 30.0% | |
| TAAATTCTTCAATCTTTGGC+TGG | - | Chr7:54648979-54648998 | None:intergenic | 30.0% | |
| TCAATATTTGACCTTTCTTG+AGG | - | Chr7:54649191-54649210 | None:intergenic | 30.0% | |
| ! | AGAAGTTGTTGTTCAAAATG+AGG | + | Chr7:54649118-54649137 | MsG0780038885.01.T01:CDS | 30.0% |
| ! | ATTTTCTTGTTCTCCAAGAA+TGG | - | Chr7:54649060-54649079 | None:intergenic | 30.0% |
| ! | TGTTGATAGGATTCATACTT+GGG | - | Chr7:54648861-54648880 | None:intergenic | 30.0% |
| ! | TTGTTGATAGGATTCATACT+TGG | - | Chr7:54648862-54648881 | None:intergenic | 30.0% |
| CCAAATATGTTTGAGAGAGT+CGG | + | Chr7:54649083-54649102 | MsG0780038885.01.T01:CDS | 35.0% | |
| GGTATCTTGCATGTTTATCA+TGG | - | Chr7:54648958-54648977 | None:intergenic | 35.0% | |
| TTCTCCATTATCAACAGTGT+TGG | - | Chr7:54649219-54649238 | None:intergenic | 35.0% | |
| ! | GTTGATTCAAGCACAAATCA+TGG | + | Chr7:54649013-54649032 | MsG0780038885.01.T01:CDS | 35.0% |
| !! | TCCAAGAATGGATTGATTGT+TGG | - | Chr7:54649048-54649067 | None:intergenic | 35.0% |
| !! | TGATAATGGAGAAGTGATAG+AGG | + | Chr7:54649226-54649245 | MsG0780038885.01.T01:CDS | 35.0% |
| AACTTTAGGTACAGCTGATG+TGG | - | Chr7:54649386-54649405 | None:intergenic | 40.0% | |
| AAGACGAACTTCCTCAAGAA+AGG | + | Chr7:54649177-54649196 | MsG0780038885.01.T01:CDS | 40.0% | |
| CCGACTCTCTCAAACATATT+TGG | - | Chr7:54649086-54649105 | None:intergenic | 40.0% | |
| CCTTGTTCAAACCAAAGTTC+AGG | + | Chr7:54649348-54649367 | MsG0780038885.01.T01:CDS | 40.0% | |
| CGCAAATTTCATCAATGACC+CGG | - | Chr7:54649328-54649347 | None:intergenic | 40.0% | |
| GCCAACAATCAATCCATTCT+TGG | + | Chr7:54649044-54649063 | MsG0780038885.01.T01:CDS | 40.0% | |
| GGAGGAATTCCATGAACTTT+AGG | - | Chr7:54649400-54649419 | None:intergenic | 40.0% | |
| TAATGGAGAAGTGATAGAGG+TGG | + | Chr7:54649229-54649248 | MsG0780038885.01.T01:CDS | 40.0% | |
| TCAGCTGTACCTAAAGTTCA+TGG | + | Chr7:54649388-54649407 | MsG0780038885.01.T01:CDS | 40.0% | |
| TGAGCAAGGCTGAAATTTAC+CGG | + | Chr7:54649306-54649325 | MsG0780038885.01.T01:CDS | 40.0% | |
| TGATAGGATTCATACTTGGG+TGG | - | Chr7:54648858-54648877 | None:intergenic | 40.0% | |
| TGTTCAAACCAAAGTTCAGG+AGG | + | Chr7:54649351-54649370 | MsG0780038885.01.T01:CDS | 40.0% | |
| ! | ATGTGTTGTCCGTCTCATTT+TGG | + | Chr7:54649444-54649463 | MsG0780038885.01.T01:CDS | 40.0% |
| !! | CCTGAACTTTGGTTTGAACA+AGG | - | Chr7:54649351-54649370 | None:intergenic | 40.0% |
| !! | GAATGGATTGATTGTTGGCT+TGG | - | Chr7:54649043-54649062 | None:intergenic | 40.0% |
| AAAGGGGAAGAAAGTGAGCA+AGG | + | Chr7:54649292-54649311 | MsG0780038885.01.T01:CDS | 45.0% | |
| AACCAAAGTTCAGGAGGTCA+TGG | + | Chr7:54649357-54649376 | MsG0780038885.01.T01:CDS | 45.0% | |
| AGAAGTGATAGAGGTGGTAG+AGG | + | Chr7:54649235-54649254 | MsG0780038885.01.T01:CDS | 45.0% | |
| AGAGGAACGGTTACTTAGCA+AGG | + | Chr7:54649253-54649272 | MsG0780038885.01.T01:CDS | 45.0% | |
| GAGCAAGGCTGAAATTTACC+GGG | + | Chr7:54649307-54649326 | MsG0780038885.01.T01:CDS | 45.0% | |
| TGAGGAAGTTCGTCTTTGTG+TGG | - | Chr7:54649173-54649192 | None:intergenic | 45.0% | |
| TGATAGAGGTGGTAGAGGAA+CGG | + | Chr7:54649240-54649259 | MsG0780038885.01.T01:CDS | 45.0% | |
| TTAGGGTGGCCAAAATGAGA+CGG | - | Chr7:54649456-54649475 | None:intergenic | 45.0% | |
| ! | GTGGCCAACACTGTTGATAA+TGG | + | Chr7:54649212-54649231 | MsG0780038885.01.T01:CDS | 45.0% |
| !! | CCTTTGAAACCCTACGCTTT+GGG | - | Chr7:54649421-54649440 | None:intergenic | 45.0% |
| !! | TCCTTTGAAACCCTACGCTT+TGG | - | Chr7:54649422-54649441 | None:intergenic | 45.0% |
| CCCAAAGCGTAGGGTTTCAA+AGG | + | Chr7:54649418-54649437 | MsG0780038885.01.T01:CDS | 50.0% | |
| GGAATTCCTCCCAAAGCGTA+GGG | + | Chr7:54649409-54649428 | MsG0780038885.01.T01:CDS | 50.0% | |
| GTCCATGACCTCCTGAACTT+TGG | - | Chr7:54649362-54649381 | None:intergenic | 50.0% | |
| TGGAATTCCTCCCAAAGCGT+AGG | + | Chr7:54649408-54649427 | MsG0780038885.01.T01:CDS | 50.0% | |
| ! | TTGAAACCCTACGCTTTGGG+AGG | - | Chr7:54649418-54649437 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 54648822 | 54649475 | 54648822 | ID=MsG0780038885.01;Name=MsG0780038885.01 |
| Chr7 | mRNA | 54648822 | 54649475 | 54648822 | ID=MsG0780038885.01.T01;Parent=MsG0780038885.01;Name=MsG0780038885.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|217 |
| Chr7 | exon | 54648822 | 54649475 | 54648822 | ID=MsG0780038885.01.T01:exon:7629;Parent=MsG0780038885.01.T01 |
| Chr7 | CDS | 54648822 | 54649475 | 54648822 | ID=MsG0780038885.01.T01:cds;Parent=MsG0780038885.01.T01 |
| Gene Sequence |
| Protein sequence |
