Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018277.01.T01 | AAL84187.1 | 87.931 | 174 | 19 | 1 | 46 | 217 | 1 | 174 | 8.78E-102 | 302 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018277.01.T01 | Q8S4Q1 | 87.931 | 174 | 19 | 1 | 46 | 217 | 1 | 174 | 4.19e-102 | 302 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002770.01 | MsG0480018277.01 | 0.884471 | 1.864901e-71 | 1.231673e-67 |
| MsG0180004025.01 | MsG0480018277.01 | 0.801069 | 1.071383e-48 | 5.069351e-46 |
| MsG0180005564.01 | MsG0480018277.01 | 0.845130 | 4.919416e-59 | 8.116676e-56 |
| MsG0480018276.01 | MsG0480018277.01 | 0.930137 | 2.534414e-93 | 1.473123e-88 |
| MsG0480018277.01 | MsG0580028264.01 | 0.852335 | 4.937814e-61 | 1.028428e-57 |
| MsG0480018277.01 | MsG0680035596.01 | 0.808030 | 3.783968e-50 | 2.140405e-47 |
| MsG0480018277.01 | MsG0680035744.01 | 0.817473 | 3.238176e-52 | 2.360617e-49 |
| MsG0480018277.01 | MsG0780038885.01 | 0.899906 | 1.245787e-77 | 1.572127e-73 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018277.01.T01 | MTR_3g030260 | 67.249 | 229 | 63 | 3 | 1 | 217 | 1 | 229 | 1.85e-91 | 268 |
| MsG0480018277.01.T01 | MTR_6g033455 | 61.905 | 231 | 74 | 4 | 1 | 217 | 1 | 231 | 4.46e-87 | 257 |
| MsG0480018277.01.T01 | MTR_2g009950 | 65.198 | 227 | 67 | 3 | 1 | 215 | 1 | 227 | 5.94e-85 | 253 |
| MsG0480018277.01.T01 | MTR_0510s0010 | 58.009 | 231 | 83 | 4 | 1 | 217 | 8 | 238 | 2.63e-77 | 232 |
| MsG0480018277.01.T01 | MTR_7g016020 | 63.212 | 193 | 59 | 3 | 1 | 181 | 1 | 193 | 1.31e-58 | 189 |
| MsG0480018277.01.T01 | MTR_2g083020 | 63.731 | 193 | 58 | 4 | 1 | 181 | 1 | 193 | 5.91e-56 | 190 |
| MsG0480018277.01.T01 | MTR_7g045350 | 62.774 | 137 | 39 | 3 | 92 | 216 | 23 | 159 | 1.79e-42 | 141 |
| MsG0480018277.01.T01 | MTR_0053s0080 | 52.632 | 190 | 77 | 3 | 1 | 177 | 1 | 190 | 3.62e-35 | 128 |
| MsG0480018277.01.T01 | MTR_8g461570 | 78.378 | 74 | 15 | 1 | 145 | 217 | 5 | 78 | 3.77e-33 | 114 |
| MsG0480018277.01.T01 | MTR_0012s0290 | 64.228 | 123 | 32 | 3 | 92 | 202 | 1147 | 1269 | 2.59e-32 | 124 |
| MsG0480018277.01.T01 | MTR_7g074720 | 80.822 | 73 | 13 | 1 | 146 | 217 | 2 | 74 | 4.78e-32 | 111 |
| MsG0480018277.01.T01 | MTR_5g454260 | 62.931 | 116 | 43 | 0 | 1 | 116 | 1 | 116 | 1.65e-29 | 110 |
| MsG0480018277.01.T01 | MTR_3g026190 | 48.598 | 107 | 50 | 2 | 66 | 167 | 825 | 931 | 1.33e-15 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGACTCTCTCAAACATATT+TGG | 0.156108 | 4:+2672536 | None:intergenic |
| GTGATAAATTCTTCAATCTT+TGG | 0.234620 | 4:+2672639 | None:intergenic |
| GGAGGAATTCCATGAACTTT+AGG | 0.268489 | 4:+2672222 | None:intergenic |
| ATGTGTTGTCCGTCTCATTT+TGG | 0.303724 | 4:-2672175 | MsG0480018277.01.T01:CDS |
| TCCTTTGAAACCCTACGCTT+TGG | 0.349397 | 4:+2672200 | None:intergenic |
| CCTTTGAAACCCTACGCTTT+GGG | 0.365038 | 4:+2672201 | None:intergenic |
| GGTATCTTGCATGTTTATCA+TGG | 0.380452 | 4:+2672664 | None:intergenic |
| AGAAGTGATAGAGGTGGTAG+AGG | 0.393182 | 4:-2672384 | MsG0480018277.01.T01:CDS |
| GGAAGAAACATTTGAACAAA+AGG | 0.395964 | 4:-2672345 | MsG0480018277.01.T01:CDS |
| TGAGCAAGGCTGAAATTTAC+CGG | 0.398275 | 4:-2672313 | MsG0480018277.01.T01:CDS |
| ACATTGTTGATATTGTTGAT+AGG | 0.404560 | 4:+2672748 | None:intergenic |
| TCGAAGAATGGATTGATTGT+TGG | 0.413463 | 4:+2672574 | None:intergenic |
| GTCCATGACCTCCTGAACTT+TGG | 0.414042 | 4:+2672260 | None:intergenic |
| TCAGCTGTACCTAAAGTTCA+TGG | 0.424076 | 4:-2672231 | MsG0480018277.01.T01:CDS |
| TTATGAAGATGAGAGATATT+CGG | 0.429317 | 4:-2672723 | MsG0480018277.01.T01:CDS |
| GTTGATTCAAGCACAAATCA+TGG | 0.442225 | 4:-2672606 | MsG0480018277.01.T01:CDS |
| CCTTGTTCAAACCAAAGTTC+AGG | 0.445067 | 4:-2672271 | MsG0480018277.01.T01:CDS |
| GTGGCCAACACTGTTGATAA+TGG | 0.457450 | 4:-2672407 | MsG0480018277.01.T01:CDS |
| TAAATTCTTCAATCTTTGGC+TGG | 0.462835 | 4:+2672643 | None:intergenic |
| GAATGGATTGATTGTTGGCT+TGG | 0.464055 | 4:+2672579 | None:intergenic |
| TTGTTGATAGGATTCATACT+TGG | 0.469352 | 4:+2672760 | None:intergenic |
| GAAGAAACATTTGAACAAAA+GGG | 0.474308 | 4:-2672344 | MsG0480018277.01.T01:CDS |
| GACAAAATCATGGCTGATCA+TGG | 0.485385 | 4:-2672806 | None:intergenic |
| AGAAGTTGTTGTTCAAAATG+AGG | 0.492337 | 4:-2672501 | MsG0480018277.01.T01:CDS |
| CCTGAACTTTGGTTTGAACA+AGG | 0.502302 | 4:+2672271 | None:intergenic |
| CGCAAATTTCATCAATGACC+CGG | 0.514753 | 4:+2672294 | None:intergenic |
| TGTTGATAGGATTCATACTT+GGG | 0.517284 | 4:+2672761 | None:intergenic |
| AACTTTAGGTACAGCTGATG+TGG | 0.527312 | 4:+2672236 | None:intergenic |
| TTCTCCATTATCAACAGTGT+TGG | 0.531055 | 4:+2672403 | None:intergenic |
| AAGACGAACTTCCTCAAGAA+AGG | 0.539436 | 4:-2672442 | MsG0480018277.01.T01:CDS |
| AACCAAAGTTCAGGAGGTCA+TGG | 0.540463 | 4:-2672262 | MsG0480018277.01.T01:CDS |
| TGGAATTCCTCCCAAAGCGT+AGG | 0.545471 | 4:-2672211 | MsG0480018277.01.T01:CDS |
| CCCAAAGCGTAGGGTTTCAA+AGG | 0.548667 | 4:-2672201 | MsG0480018277.01.T01:CDS |
| TCAATATTTGACCTTTCTTG+AGG | 0.555926 | 4:+2672431 | None:intergenic |
| CCAAATATGTTTGAGAGAGT+CGG | 0.559778 | 4:-2672536 | MsG0480018277.01.T01:CDS |
| GGAATTCCTCCCAAAGCGTA+GGG | 0.566799 | 4:-2672210 | MsG0480018277.01.T01:CDS |
| TGATAATGGAGAAGTGATAG+AGG | 0.583165 | 4:-2672393 | MsG0480018277.01.T01:CDS |
| AGAAAGGTCAAATATTGAAG+TGG | 0.590226 | 4:-2672426 | MsG0480018277.01.T01:CDS |
| AGAGGAACGGTTACTTAGCA+AGG | 0.590261 | 4:-2672366 | MsG0480018277.01.T01:CDS |
| AAGAAACATTTGAACAAAAG+GGG | 0.596132 | 4:-2672343 | MsG0480018277.01.T01:CDS |
| TGATAGGATTCATACTTGGG+TGG | 0.604185 | 4:+2672764 | None:intergenic |
| TTGAAACCCTACGCTTTGGG+AGG | 0.607057 | 4:+2672204 | None:intergenic |
| TGATAGAGGTGGTAGAGGAA+CGG | 0.612805 | 4:-2672379 | MsG0480018277.01.T01:CDS |
| GTTACAACATTAGAAGAATG+TGG | 0.625446 | 4:-2672476 | MsG0480018277.01.T01:CDS |
| TAATGGAGAAGTGATAGAGG+TGG | 0.631937 | 4:-2672390 | MsG0480018277.01.T01:CDS |
| TGAGGAAGTTCGTCTTTGTG+TGG | 0.633527 | 4:+2672449 | None:intergenic |
| AAAGGGGAAGAAAGTGAGCA+AGG | 0.636089 | 4:-2672327 | MsG0480018277.01.T01:CDS |
| TGTTCAAACCAAAGTTCAGG+AGG | 0.642083 | 4:-2672268 | MsG0480018277.01.T01:CDS |
| GAGCAAGGCTGAAATTTACC+GGG | 0.643159 | 4:-2672312 | MsG0480018277.01.T01:CDS |
| TTAGGGCGGCCAAAATGAGA+CGG | 0.676286 | 4:+2672166 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAACATTTGAACAAAAG+GGG | - | Chr4:2672620-2672639 | MsG0480018277.01.T01:CDS | 25.0% |
| ! | ACATTGTTGATATTGTTGAT+AGG | + | Chr4:2672218-2672237 | None:intergenic | 25.0% |
| ! | GAAGAAACATTTGAACAAAA+GGG | - | Chr4:2672619-2672638 | MsG0480018277.01.T01:CDS | 25.0% |
| ! | GTGATAAATTCTTCAATCTT+TGG | + | Chr4:2672327-2672346 | None:intergenic | 25.0% |
| ! | TTATGAAGATGAGAGATATT+CGG | - | Chr4:2672240-2672259 | MsG0480018277.01.T01:CDS | 25.0% |
| AGAAAGGTCAAATATTGAAG+TGG | - | Chr4:2672537-2672556 | MsG0480018277.01.T01:CDS | 30.0% | |
| GGAAGAAACATTTGAACAAA+AGG | - | Chr4:2672618-2672637 | MsG0480018277.01.T01:CDS | 30.0% | |
| GTTACAACATTAGAAGAATG+TGG | - | Chr4:2672487-2672506 | MsG0480018277.01.T01:CDS | 30.0% | |
| TAAATTCTTCAATCTTTGGC+TGG | + | Chr4:2672323-2672342 | None:intergenic | 30.0% | |
| TCAATATTTGACCTTTCTTG+AGG | + | Chr4:2672535-2672554 | None:intergenic | 30.0% | |
| ! | AGAAGTTGTTGTTCAAAATG+AGG | - | Chr4:2672462-2672481 | MsG0480018277.01.T01:CDS | 30.0% |
| ! | ATTTTCTTGTTCTCGAAGAA+TGG | + | Chr4:2672404-2672423 | None:intergenic | 30.0% |
| ! | TGTTGATAGGATTCATACTT+GGG | + | Chr4:2672205-2672224 | None:intergenic | 30.0% |
| ! | TTGTTGATAGGATTCATACT+TGG | + | Chr4:2672206-2672225 | None:intergenic | 30.0% |
| CCAAATATGTTTGAGAGAGT+CGG | - | Chr4:2672427-2672446 | MsG0480018277.01.T01:CDS | 35.0% | |
| GGTATCTTGCATGTTTATCA+TGG | + | Chr4:2672302-2672321 | None:intergenic | 35.0% | |
| TTCTCCATTATCAACAGTGT+TGG | + | Chr4:2672563-2672582 | None:intergenic | 35.0% | |
| ! | GTTGATTCAAGCACAAATCA+TGG | - | Chr4:2672357-2672376 | MsG0480018277.01.T01:CDS | 35.0% |
| !! | TCGAAGAATGGATTGATTGT+TGG | + | Chr4:2672392-2672411 | None:intergenic | 35.0% |
| !! | TGATAATGGAGAAGTGATAG+AGG | - | Chr4:2672570-2672589 | MsG0480018277.01.T01:CDS | 35.0% |
| AACTTTAGGTACAGCTGATG+TGG | + | Chr4:2672730-2672749 | None:intergenic | 40.0% | |
| AAGACGAACTTCCTCAAGAA+AGG | - | Chr4:2672521-2672540 | MsG0480018277.01.T01:CDS | 40.0% | |
| CCGACTCTCTCAAACATATT+TGG | + | Chr4:2672430-2672449 | None:intergenic | 40.0% | |
| CCTTGTTCAAACCAAAGTTC+AGG | - | Chr4:2672692-2672711 | MsG0480018277.01.T01:CDS | 40.0% | |
| CGCAAATTTCATCAATGACC+CGG | + | Chr4:2672672-2672691 | None:intergenic | 40.0% | |
| GGAGGAATTCCATGAACTTT+AGG | + | Chr4:2672744-2672763 | None:intergenic | 40.0% | |
| TAATGGAGAAGTGATAGAGG+TGG | - | Chr4:2672573-2672592 | MsG0480018277.01.T01:CDS | 40.0% | |
| TCAGCTGTACCTAAAGTTCA+TGG | - | Chr4:2672732-2672751 | MsG0480018277.01.T01:CDS | 40.0% | |
| TGAGCAAGGCTGAAATTTAC+CGG | - | Chr4:2672650-2672669 | MsG0480018277.01.T01:CDS | 40.0% | |
| TGATAGGATTCATACTTGGG+TGG | + | Chr4:2672202-2672221 | None:intergenic | 40.0% | |
| TGTTCAAACCAAAGTTCAGG+AGG | - | Chr4:2672695-2672714 | MsG0480018277.01.T01:CDS | 40.0% | |
| ! | ATGTGTTGTCCGTCTCATTT+TGG | - | Chr4:2672788-2672807 | MsG0480018277.01.T01:CDS | 40.0% |
| !! | CCTGAACTTTGGTTTGAACA+AGG | + | Chr4:2672695-2672714 | None:intergenic | 40.0% |
| !! | GAATGGATTGATTGTTGGCT+TGG | + | Chr4:2672387-2672406 | None:intergenic | 40.0% |
| AAAGGGGAAGAAAGTGAGCA+AGG | - | Chr4:2672636-2672655 | MsG0480018277.01.T01:CDS | 45.0% | |
| AACCAAAGTTCAGGAGGTCA+TGG | - | Chr4:2672701-2672720 | MsG0480018277.01.T01:CDS | 45.0% | |
| AGAAGTGATAGAGGTGGTAG+AGG | - | Chr4:2672579-2672598 | MsG0480018277.01.T01:CDS | 45.0% | |
| AGAGGAACGGTTACTTAGCA+AGG | - | Chr4:2672597-2672616 | MsG0480018277.01.T01:CDS | 45.0% | |
| GAGCAAGGCTGAAATTTACC+GGG | - | Chr4:2672651-2672670 | MsG0480018277.01.T01:CDS | 45.0% | |
| TGAGGAAGTTCGTCTTTGTG+TGG | + | Chr4:2672517-2672536 | None:intergenic | 45.0% | |
| TGATAGAGGTGGTAGAGGAA+CGG | - | Chr4:2672584-2672603 | MsG0480018277.01.T01:CDS | 45.0% | |
| ! | GTGGCCAACACTGTTGATAA+TGG | - | Chr4:2672556-2672575 | MsG0480018277.01.T01:CDS | 45.0% |
| !! | CCTTTGAAACCCTACGCTTT+GGG | + | Chr4:2672765-2672784 | None:intergenic | 45.0% |
| !! | TCCTTTGAAACCCTACGCTT+TGG | + | Chr4:2672766-2672785 | None:intergenic | 45.0% |
| CCCAAAGCGTAGGGTTTCAA+AGG | - | Chr4:2672762-2672781 | MsG0480018277.01.T01:CDS | 50.0% | |
| GGAATTCCTCCCAAAGCGTA+GGG | - | Chr4:2672753-2672772 | MsG0480018277.01.T01:CDS | 50.0% | |
| GTCCATGACCTCCTGAACTT+TGG | + | Chr4:2672706-2672725 | None:intergenic | 50.0% | |
| TGGAATTCCTCCCAAAGCGT+AGG | - | Chr4:2672752-2672771 | MsG0480018277.01.T01:CDS | 50.0% | |
| TTAGGGCGGCCAAAATGAGA+CGG | + | Chr4:2672800-2672819 | None:intergenic | 50.0% | |
| ! | TTGAAACCCTACGCTTTGGG+AGG | + | Chr4:2672762-2672781 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 2672166 | 2672819 | 2672166 | ID=MsG0480018277.01;Name=MsG0480018277.01 |
| Chr4 | mRNA | 2672166 | 2672819 | 2672166 | ID=MsG0480018277.01.T01;Parent=MsG0480018277.01;Name=MsG0480018277.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|217 |
| Chr4 | exon | 2672166 | 2672819 | 2672166 | ID=MsG0480018277.01.T01:exon:819;Parent=MsG0480018277.01.T01 |
| Chr4 | CDS | 2672166 | 2672819 | 2672166 | ID=MsG0480018277.01.T01:cds;Parent=MsG0480018277.01.T01 |
| Gene Sequence |
| Protein sequence |
