Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005704.01.T01 | KEH43970.1 | 92.683 | 41 | 3 | 0 | 1 | 41 | 158 | 198 | 7.19E-17 | 83.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005704.01.T01 | A0A072W108 | 92.683 | 41 | 3 | 0 | 1 | 41 | 158 | 198 | 3.43e-17 | 83.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005146.01 | MsG0180005704.01 | 0.804856 | 1.767182e-49 | 9.211018e-47 |
| MsG0180005704.01 | MsG0380017200.01 | 0.820130 | 8.068891e-53 | 6.330931e-50 |
| MsG0180005704.01 | MsG0380017307.01 | 0.817491 | 3.208001e-52 | 2.339701e-49 |
| MsG0180005704.01 | MsG0780041447.01 | 0.806791 | 6.928828e-50 | 3.794952e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005704.01.T01 | MTR_1g105860 | 92.683 | 41 | 3 | 0 | 1 | 41 | 158 | 198 | 8.71e-21 | 83.2 |
| MsG0180005704.01.T01 | MTR_1g105860 | 92.683 | 41 | 3 | 0 | 1 | 41 | 175 | 215 | 2.20e-20 | 82.4 |
| MsG0180005704.01.T01 | MTR_1g105885 | 75.610 | 41 | 10 | 0 | 1 | 41 | 155 | 195 | 3.38e-17 | 73.6 |
| MsG0180005704.01.T01 | MTR_1g105855 | 82.927 | 41 | 7 | 0 | 1 | 41 | 175 | 215 | 4.77e-17 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAGATCAATTTAATATCT+TGG | 0.285791 | 1:+95200499 | MsG0180005704.01.T01:CDS |
| TTTCAAGATGTTGTAGCAAT+AGG | 0.335675 | 1:+95200701 | MsG0180005704.01.T01:CDS |
| TTAAGAAGCAAGGCTATAAA+TGG | 0.352413 | 1:+95200542 | MsG0180005704.01.T01:CDS |
| TTAGACCAAGCTGTAATTTG+AGG | 0.412757 | 1:+95200740 | MsG0180005704.01.T01:CDS |
| CAGTGTACACCTGACTTGTC+TGG | 0.440744 | 1:+95200637 | MsG0180005704.01.T01:CDS |
| TCACATCGTAAGTATGTTGT+AGG | 0.450527 | 1:+95200569 | MsG0180005704.01.T01:CDS |
| ATATATATTTGATTACTACT+AGG | 0.471336 | 1:-95200605 | None:intergenic |
| TAGTAGTAATCAAATATATA+TGG | 0.476766 | 1:+95200607 | MsG0180005704.01.T01:CDS |
| GTTGAATGATTTAAGAAGCA+AGG | 0.524724 | 1:+95200532 | MsG0180005704.01.T01:CDS |
| TCAAACCTCAAATTACAGCT+TGG | 0.526400 | 1:-95200745 | None:intergenic |
| AGATGTTGTAGCAATAGGAT+TGG | 0.527777 | 1:+95200706 | MsG0180005704.01.T01:CDS |
| CACTGGGGACCAGACAAGTC+AGG | 0.531473 | 1:-95200646 | None:intergenic |
| AAGCAAACAATCATCACACT+GGG | 0.585932 | 1:-95200662 | None:intergenic |
| GAAGCAAACAATCATCACAC+TGG | 0.591047 | 1:-95200663 | None:intergenic |
| TATGTTGTAGGAACAATGAC+GGG | 0.594989 | 1:+95200581 | MsG0180005704.01.T01:CDS |
| GTATGTTGTAGGAACAATGA+CGG | 0.611472 | 1:+95200580 | MsG0180005704.01.T01:CDS |
| AGCAAACAATCATCACACTG+GGG | 0.800516 | 1:-95200661 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATTTGATTACTACT+AGG | - | Chr1:95200608-95200627 | None:intergenic | 15.0% |
| !! | TAGTAGTAATCAAATATATA+TGG | + | Chr1:95200607-95200626 | MsG0180005704.01.T01:CDS | 15.0% |
| GGCAGATCAATTTAATATCT+TGG | + | Chr1:95200499-95200518 | MsG0180005704.01.T01:CDS | 30.0% | |
| GTTGAATGATTTAAGAAGCA+AGG | + | Chr1:95200532-95200551 | MsG0180005704.01.T01:CDS | 30.0% | |
| TTAAGAAGCAAGGCTATAAA+TGG | + | Chr1:95200542-95200561 | MsG0180005704.01.T01:CDS | 30.0% | |
| TTTCAAGATGTTGTAGCAAT+AGG | + | Chr1:95200701-95200720 | MsG0180005704.01.T01:CDS | 30.0% | |
| AAGCAAACAATCATCACACT+GGG | - | Chr1:95200665-95200684 | None:intergenic | 35.0% | |
| AGATGTTGTAGCAATAGGAT+TGG | + | Chr1:95200706-95200725 | MsG0180005704.01.T01:CDS | 35.0% | |
| GTATGTTGTAGGAACAATGA+CGG | + | Chr1:95200580-95200599 | MsG0180005704.01.T01:CDS | 35.0% | |
| TATGTTGTAGGAACAATGAC+GGG | + | Chr1:95200581-95200600 | MsG0180005704.01.T01:CDS | 35.0% | |
| TCACATCGTAAGTATGTTGT+AGG | + | Chr1:95200569-95200588 | MsG0180005704.01.T01:CDS | 35.0% | |
| AGCAAACAATCATCACACTG+GGG | - | Chr1:95200664-95200683 | None:intergenic | 40.0% | |
| GAAGCAAACAATCATCACAC+TGG | - | Chr1:95200666-95200685 | None:intergenic | 40.0% | |
| ! | CAGTGTACACCTGACTTGTC+TGG | + | Chr1:95200637-95200656 | MsG0180005704.01.T01:CDS | 50.0% |
| CACTGGGGACCAGACAAGTC+AGG | - | Chr1:95200649-95200668 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 95200497 | 95200760 | 95200497 | ID=MsG0180005704.01;Name=MsG0180005704.01 |
| Chr1 | mRNA | 95200497 | 95200760 | 95200497 | ID=MsG0180005704.01.T01;Parent=MsG0180005704.01;Name=MsG0180005704.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|87 |
| Chr1 | exon | 95200497 | 95200760 | 95200497 | ID=MsG0180005704.01.T01:exon:10398;Parent=MsG0180005704.01.T01 |
| Chr1 | CDS | 95200497 | 95200760 | 95200497 | ID=MsG0180005704.01.T01:cds;Parent=MsG0180005704.01.T01 |
| Gene Sequence |
| Protein sequence |
