AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005814.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005814.01.T01 MTR_1g107430 100.000 71 0 0 186 256 59 129 1.23e-44 151
MsG0180005814.01.T01 MTR_1g107430 88.462 52 6 0 205 256 10 61 9.87e-23 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005814.01.T01 AT2G15240 68.675 83 21 1 179 256 42 124 9.58e-25 99.4

Find 45 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATTGACTGGCATATTTC+TGG 0.151764 1:-96525263 MsG0180005814.01.T01:CDS
CTATATACTTACACAACTTT+TGG 0.255052 1:+96526619 None:intergenic
ATCGCGTGCCCATTGATTCT+TGG 0.282291 1:+96525772 None:intergenic
TGTGATCTGCAGCCTTCTTT+TGG 0.305006 1:-96525735 MsG0180005814.01.T01:CDS
CAACAGCATTTGCAATGAAA+TGG 0.310155 1:+96527811 None:intergenic
GAAGTGAATTAGCGAAATTA+AGG 0.315424 1:+96527847 None:intergenic
ATATGCCAGTCAATAGAAAA+TGG 0.326818 1:+96525269 None:intergenic
TTCAGGTATGACCATAGTTC+TGG 0.341007 1:-96525328 MsG0180005814.01.T01:intron
GTAAGGAAGAAGAGTGGAAT+TGG 0.353691 1:+96527924 None:intergenic
AACAGCATTTGCAATGAAAT+GGG 0.370345 1:+96527812 None:intergenic
CCTTTGGTTGTGTATGTGTT+TGG 0.377398 1:-96526429 MsG0180005814.01.T01:CDS
TACTCACCCAGTCACCCCTT+TGG 0.410451 1:-96526445 MsG0180005814.01.T01:intron
GAAGAAATGAAGAGATCAGT+TGG 0.431029 1:+96527879 None:intergenic
AAGTGAATTAGCGAAATTAA+GGG 0.438229 1:+96527848 None:intergenic
TTCATTGTAGTGGCAACAAA+TGG 0.444628 1:-96526686 MsG0180005814.01.T01:intron
CTGCAGATCACAACAAATGC+AGG 0.457525 1:+96525746 None:intergenic
AATTAGAAACCAAGAATCAA+TGG 0.462371 1:-96525781 MsG0180005814.01.T01:intron
AAAGTGCATGGCCAGAACTA+TGG 0.469079 1:+96525317 None:intergenic
TTGATAATTCTGCGGAGGTA+AGG 0.492063 1:+96527907 None:intergenic
ATTGTTGTTGCATGAGATCA+TGG 0.493519 1:+96526539 None:intergenic
TTACCTCCGCAGAATTATCA+AGG 0.501424 1:-96527905 MsG0180005814.01.T01:CDS
GGGTCGAGAGGAAGATGAAG+AGG 0.502177 1:+96527945 None:intergenic
TTTGCAATGAAATGGGGAAT+CGG 0.502682 1:+96527819 None:intergenic
TAAGGAAGAAGAGTGGAATT+GGG 0.507072 1:+96527925 None:intergenic
AAGAGTGGAATTGGGTCGAG+AGG 0.513571 1:+96527933 None:intergenic
ATTAGAAACCAAGAATCAAT+GGG 0.517637 1:-96525780 MsG0180005814.01.T01:intron
CAAACACATACACAACCAAA+GGG 0.527703 1:+96526430 None:intergenic
GAGGGGTCAATCTAATCCTA+TGG 0.531341 1:+96527766 None:intergenic
AAGAAATGAAGAGATCAGTT+GGG 0.537604 1:+96527880 None:intergenic
AAGTTGCAACAGCCAAAAGA+AGG 0.559727 1:+96525723 None:intergenic
CCAAACACATACACAACCAA+AGG 0.571051 1:+96526429 None:intergenic
GAGCGTCCTTTCGAAACTGT+TGG 0.575354 1:+96527979 None:intergenic
TTGCAAATGCTGTTGAATTG+TGG 0.577184 1:-96527803 MsG0180005814.01.T01:CDS
CTTGATATGGAACTGTGCAA+TGG 0.612580 1:-96526366 MsG0180005814.01.T01:CDS
ATGTTACCAACAGTTTCGAA+AGG 0.623237 1:-96527985 MsG0180005814.01.T01:CDS
ACAGCATTTGCAATGAAATG+GGG 0.627788 1:+96527813 None:intergenic
TGCAGATCACAACAAATGCA+GGG 0.638452 1:+96525747 None:intergenic
GTACCTTGATAATTCTGCGG+AGG 0.646223 1:+96527902 None:intergenic
GGCGCAATATTATAAACCAT+AGG 0.656207 1:-96527782 MsG0180005814.01.T01:CDS
TGGGTACCTTGATAATTCTG+CGG 0.658546 1:+96527899 None:intergenic
GCGGAGGTAAGGAAGAAGAG+TGG 0.669478 1:+96527918 None:intergenic
ACAGTGCTCAAGTAATCACG+AGG 0.680267 1:+96527747 None:intergenic
CAGTGCTCAAGTAATCACGA+GGG 0.716478 1:+96527748 None:intergenic
AGTGCTCAAGTAATCACGAG+GGG 0.729515 1:+96527749 None:intergenic
AAACACATACACAACCAAAG+GGG 0.739177 1:+96526431 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAATCCAAAATATTA+AGG + Chr1:96526123-96526142 None:intergenic 10.0%
!! TACATTTAATCAAATAAAAT+AGG + Chr1:96526738-96526757 None:intergenic 10.0%
!! AAAAAGTAGATAATAGAAAA+TGG + Chr1:96525829-96525848 None:intergenic 15.0%
!!! AATGGATTTTTCTAAATAAA+AGG + Chr1:96525811-96525830 None:intergenic 15.0%
!!! AATTTTATTATTCATTGTAG+TGG - Chr1:96526549-96526568 MsG0180005814.01.T01:CDS 15.0%
!!! ATTTTGTTTTCTTTTCAATT+TGG - Chr1:96526362-96526381 MsG0180005814.01.T01:CDS 15.0%
!!! TTGGATTATAATTTTTTGAA+TGG - Chr1:96526130-96526149 MsG0180005814.01.T01:intron 15.0%
!! AAAATCATAATATTCTCAGT+AGG - Chr1:96526287-96526306 MsG0180005814.01.T01:intron 20.0%
!! AATTAACTGCAACATTTAAA+TGG + Chr1:96527262-96527281 None:intergenic 20.0%
!! TCTTGATGTTAAAAAAAAGA+TGG + Chr1:96526667-96526686 None:intergenic 20.0%
!! TTTGTAGATCATATATTTAG+TGG - Chr1:96527675-96527694 MsG0180005814.01.T01:intron 20.0%
!!! ATAACATGTTTCTATAAGAA+TGG + Chr1:96527724-96527743 None:intergenic 20.0%
!!! TAAACAAAGCCTTAATATTT+TGG - Chr1:96526111-96526130 MsG0180005814.01.T01:intron 20.0%
!!! TCTGTATTGTTTTGATTTAT+TGG - Chr1:96526956-96526975 MsG0180005814.01.T01:intron 20.0%
! AAGTGAATTAGCGAAATTAA+GGG + Chr1:96525400-96525419 None:intergenic 25.0%
! AATTAGAAACCAAGAATCAA+TGG - Chr1:96527464-96527483 MsG0180005814.01.T01:intron 25.0%
! AGATCATATATTTAGTGGTA+GGG - Chr1:96527680-96527699 MsG0180005814.01.T01:intron 25.0%
! ATGTAACTATATAGTGTTTC+TGG - Chr1:96527347-96527366 MsG0180005814.01.T01:intron 25.0%
! ATTAGAAACCAAGAATCAAT+GGG - Chr1:96527465-96527484 MsG0180005814.01.T01:intron 25.0%
! CATGTTATCACTTTAAACTT+TGG - Chr1:96527736-96527755 MsG0180005814.01.T01:CDS 25.0%
! GGATAATTAATTGCAGATAA+AGG + Chr1:96527059-96527078 None:intergenic 25.0%
! GTGTTAGCATAGAAAATAAA+GGG - Chr1:96527113-96527132 MsG0180005814.01.T01:intron 25.0%
! TAAAAGCGAACACATAATTT+AGG + Chr1:96525613-96525632 None:intergenic 25.0%
! TAAAATTACAGAGAACGAAA+AGG + Chr1:96526536-96526555 None:intergenic 25.0%
! TAAATAAAAGGTTCACACAA+AGG + Chr1:96525799-96525818 None:intergenic 25.0%
! TAGATCATATATTTAGTGGT+AGG - Chr1:96527679-96527698 MsG0180005814.01.T01:intron 25.0%
! TGTTAGCATAGAAAATAAAG+GGG - Chr1:96527114-96527133 MsG0180005814.01.T01:intron 25.0%
!! CTATATACTTACACAACTTT+TGG + Chr1:96526629-96526648 None:intergenic 25.0%
!! TAATTTTCCCATTCATTTTC+AGG - Chr1:96527900-96527919 MsG0180005814.01.T01:CDS 25.0%
!! TGCAGTTAATTCTTTTGATA+AGG - Chr1:96527271-96527290 MsG0180005814.01.T01:intron 25.0%
!! TTCTTTTCAATTTGGTATAG+AGG - Chr1:96526370-96526389 MsG0180005814.01.T01:CDS 25.0%
!!! ATTGGTACATGTTTTAAACA+GGG - Chr1:96526974-96526993 MsG0180005814.01.T01:intron 25.0%
!!! TATTGGTACATGTTTTAAAC+AGG - Chr1:96526973-96526992 MsG0180005814.01.T01:intron 25.0%
AAATATCCTGCATAAATTGG+AGG - Chr1:96527157-96527176 MsG0180005814.01.T01:intron 30.0%
AACAGCATTTGCAATGAAAT+GGG + Chr1:96525436-96525455 None:intergenic 30.0%
AACTAGAAGATGTTAGAATC+TGG - Chr1:96525639-96525658 MsG0180005814.01.T01:intron 30.0%
AAGAAATGAAGAGATCAGTT+GGG + Chr1:96525368-96525387 None:intergenic 30.0%
ATATGCCAGTCAATAGAAAA+TGG + Chr1:96527979-96527998 None:intergenic 30.0%
CGTGTTAGCATAGAAAATAA+AGG - Chr1:96527112-96527131 MsG0180005814.01.T01:intron 30.0%
GAAGTGAATTAGCGAAATTA+AGG + Chr1:96525401-96525420 None:intergenic 30.0%
GAGAAATATCCTGCATAAAT+TGG - Chr1:96527154-96527173 MsG0180005814.01.T01:intron 30.0%
GGCACTTAATGAACTAAATA+GGG + Chr1:96527847-96527866 None:intergenic 30.0%
TAATCGTGAATATTGAACTG+TGG - Chr1:96527776-96527795 MsG0180005814.01.T01:CDS 30.0%
TAATTATCCACAGTGCATTA+TGG - Chr1:96527070-96527089 MsG0180005814.01.T01:intron 30.0%
TCAATAATTCATGAACTGAG+CGG + Chr1:96525535-96525554 None:intergenic 30.0%
TCACACTAGTTATATATTCC+TGG + Chr1:96527652-96527671 None:intergenic 30.0%
TTTACATTTGTAGAACAACC+AGG - Chr1:96527631-96527650 MsG0180005814.01.T01:intron 30.0%
! CTATTCCATTTTCTATTGAC+TGG - Chr1:96527971-96527990 MsG0180005814.01.T01:CDS 30.0%
! TGGATGTTGAATACACTTTT+TGG - Chr1:96526579-96526598 MsG0180005814.01.T01:CDS 30.0%
!! TACATGTTTTAAACAGGGTT+TGG - Chr1:96526979-96526998 MsG0180005814.01.T01:intron 30.0%
!!! ATCTACTTTTTTGTGTGAAC+CGG - Chr1:96525838-96525857 MsG0180005814.01.T01:intron 30.0%
!!! TATTTCTGGCAACTTTTTGT+TGG - Chr1:96527996-96528015 MsG0180005814.01.T01:CDS 30.0%
!!! TTTGCTGTATTTTTTGACTC+AGG - Chr1:96526495-96526514 MsG0180005814.01.T01:intron 30.0%
AAACACATACACAACCAAAG+GGG + Chr1:96526817-96526836 None:intergenic 35.0%
ACAGCATTTGCAATGAAATG+GGG + Chr1:96525435-96525454 None:intergenic 35.0%
ATAATAGTCTCATTCCCCAA+TGG - Chr1:96526198-96526217 MsG0180005814.01.T01:intron 35.0%
ATACAGATATTCGTGAGAAG+TGG + Chr1:96526943-96526962 None:intergenic 35.0%
ATGGTGTGCTATGTATGATA+CGG - Chr1:96526167-96526186 MsG0180005814.01.T01:intron 35.0%
ATGTTACCAACAGTTTCGAA+AGG - Chr1:96525260-96525279 MsG0180005814.01.T01:CDS 35.0%
CAAACACATACACAACCAAA+GGG + Chr1:96526818-96526837 None:intergenic 35.0%
CAACAGCATTTGCAATGAAA+TGG + Chr1:96525437-96525456 None:intergenic 35.0%
GAAGAAATGAAGAGATCAGT+TGG + Chr1:96525369-96525388 None:intergenic 35.0%
GCTTGTACATGAACTTTGTT+TGG - Chr1:96527320-96527339 MsG0180005814.01.T01:intron 35.0%
GGCGCAATATTATAAACCAT+AGG - Chr1:96525463-96525482 MsG0180005814.01.T01:intron 35.0%
GGGCACTTAATGAACTAAAT+AGG + Chr1:96527848-96527867 None:intergenic 35.0%
GGTCATACCTGAAAATGAAT+GGG + Chr1:96527910-96527929 None:intergenic 35.0%
TAAGGAAGAAGAGTGGAATT+GGG + Chr1:96525323-96525342 None:intergenic 35.0%
TAAGTAAACTGCATAGCACA+AGG + Chr1:96526457-96526476 None:intergenic 35.0%
TAGCTAGTCATCTAATCATG+AGG + Chr1:96527597-96527616 None:intergenic 35.0%
TATCTCTTCCAGTTTAACCA+GGG + Chr1:96525989-96526008 None:intergenic 35.0%
TCATATATTTAGTGGTAGGG+TGG - Chr1:96527683-96527702 MsG0180005814.01.T01:intron 35.0%
TGCATAAATTGGAGGAAACA+CGG - Chr1:96527165-96527184 MsG0180005814.01.T01:intron 35.0%
TGGTCATACCTGAAAATGAA+TGG + Chr1:96527911-96527930 None:intergenic 35.0%
TTCATTGTAGTGGCAACAAA+TGG - Chr1:96526559-96526578 MsG0180005814.01.T01:CDS 35.0%
TTGCAAATGCTGTTGAATTG+TGG - Chr1:96525442-96525461 MsG0180005814.01.T01:intron 35.0%
TTTGCAATGAAATGGGGAAT+CGG + Chr1:96525429-96525448 None:intergenic 35.0%
! AAGTTTCTCATGCATTTTGG+CGG - Chr1:96527563-96527582 MsG0180005814.01.T01:intron 35.0%
! AGAAGACAACAAAAAGTGCA+TGG + Chr1:96527943-96527962 None:intergenic 35.0%
! ATTGTTGTTGCATGAGATCA+TGG + Chr1:96526709-96526728 None:intergenic 35.0%
! CGTAAGTTTCTCATGCATTT+TGG - Chr1:96527560-96527579 MsG0180005814.01.T01:intron 35.0%
! CGTTTTTCGTACACTTGATA+TGG - Chr1:96526866-96526885 MsG0180005814.01.T01:intron 35.0%
! CTAAGGAACTTTGGCTTATT+AGG - Chr1:96527298-96527317 MsG0180005814.01.T01:intron 35.0%
! GAAGGAAAAGCATAATGTAG+AGG - Chr1:96526262-96526281 MsG0180005814.01.T01:intron 35.0%
! TCTATTGACTGGCATATTTC+TGG - Chr1:96527982-96528001 MsG0180005814.01.T01:CDS 35.0%
! TCTTTTGATAAGGAGCTCTA+AGG - Chr1:96527281-96527300 MsG0180005814.01.T01:intron 35.0%
! TGCTTTTCCTTCATTTTCCA+CGG + Chr1:96526254-96526273 None:intergenic 35.0%
!!! TCTACTTTTTTGTGTGAACC+GGG - Chr1:96525839-96525858 MsG0180005814.01.T01:intron 35.0%
!!! TTTTTTGAATGGTGTGTGTG+TGG - Chr1:96526141-96526160 MsG0180005814.01.T01:intron 35.0%
AAGTTGCAACAGCCAAAAGA+AGG + Chr1:96527525-96527544 None:intergenic 40.0%
AGCTGTGTTAAATCTCTTGC+GGG + Chr1:96525935-96525954 None:intergenic 40.0%
ATCTCTTCCAGTTTAACCAG+GGG + Chr1:96525988-96526007 None:intergenic 40.0%
CAGCTGTGTTAAATCTCTTG+CGG + Chr1:96525936-96525955 None:intergenic 40.0%
CCAAACACATACACAACCAA+AGG + Chr1:96526819-96526838 None:intergenic 40.0%
CCTTTGGTTGTGTATGTGTT+TGG - Chr1:96526816-96526835 MsG0180005814.01.T01:intron 40.0%
CTCAACCAACCATCAGTTTA+TGG + Chr1:96526776-96526795 None:intergenic 40.0%
CTTCTATTCCCTACACCATT+GGG + Chr1:96526216-96526235 None:intergenic 40.0%
CTTGATATGGAACTGTGCAA+TGG - Chr1:96526879-96526898 MsG0180005814.01.T01:intron 40.0%
GGATAGAGGGGAATAATGAT+GGG - Chr1:96527186-96527205 MsG0180005814.01.T01:intron 40.0%
GTAAAACTGACAGCAGCTAA+TGG + Chr1:96526320-96526339 None:intergenic 40.0%
GTAACTACCATAATGCACTG+TGG + Chr1:96527080-96527099 None:intergenic 40.0%
GTAAGGAAGAAGAGTGGAAT+TGG + Chr1:96525324-96525343 None:intergenic 40.0%
GTATCTCTTCCAGTTTAACC+AGG + Chr1:96525990-96526009 None:intergenic 40.0%
GTGTTTCCTCCAATTTATGC+AGG + Chr1:96527166-96527185 None:intergenic 40.0%
TAAATGTACCACCAAACGCA+TGG - Chr1:96526750-96526769 MsG0180005814.01.T01:intron 40.0%
TAGTTCATTAAGTGCCCCTA+GGG - Chr1:96527851-96527870 MsG0180005814.01.T01:CDS 40.0%
TCTTCTATTCCCTACACCAT+TGG + Chr1:96526217-96526236 None:intergenic 40.0%
TGAGATACCGTGGAAAATGA+AGG - Chr1:96526244-96526263 MsG0180005814.01.T01:intron 40.0%
TGCAGATCACAACAAATGCA+GGG + Chr1:96527501-96527520 None:intergenic 40.0%
TGGTGTGCTATGTATGATAC+GGG - Chr1:96526168-96526187 MsG0180005814.01.T01:intron 40.0%
TTACCTCCGCAGAATTATCA+AGG - Chr1:96525340-96525359 MsG0180005814.01.T01:intron 40.0%
TTAGTTCATTAAGTGCCCCT+AGG - Chr1:96527850-96527869 MsG0180005814.01.T01:CDS 40.0%
TTCAGGTATGACCATAGTTC+TGG - Chr1:96527917-96527936 MsG0180005814.01.T01:CDS 40.0%
TTCTATTCCCTACACCATTG+GGG + Chr1:96526215-96526234 None:intergenic 40.0%
! TGGGTACCTTGATAATTCTG+CGG + Chr1:96525349-96525368 None:intergenic 40.0%
! TTGATAATTCTGCGGAGGTA+AGG + Chr1:96525341-96525360 None:intergenic 40.0%
!! TAAGGAGCTCTAAGGAACTT+TGG - Chr1:96527289-96527308 MsG0180005814.01.T01:intron 40.0%
!! TCTTTTGGCTGTTGCAACTT+TGG - Chr1:96527525-96527544 MsG0180005814.01.T01:intron 40.0%
!! TGGTGCTTTTCTGGGAATAA+TGG - Chr1:96525698-96525717 MsG0180005814.01.T01:intron 40.0%
AAAGTGCATGGCCAGAACTA+TGG + Chr1:96527931-96527950 None:intergenic 45.0%
AAATGTTAGCAGATGCCCCT+AGG + Chr1:96527870-96527889 None:intergenic 45.0%
AACGCATGGCCATAAACTGA+TGG - Chr1:96526764-96526783 MsG0180005814.01.T01:intron 45.0%
AATGTTAGCAGATGCCCCTA+GGG + Chr1:96527869-96527888 None:intergenic 45.0%
ACAGTGCTCAAGTAATCACG+AGG + Chr1:96525501-96525520 None:intergenic 45.0%
AGTGCTCAAGTAATCACGAG+GGG + Chr1:96525499-96525518 None:intergenic 45.0%
AGTTCATTAAGTGCCCCTAG+GGG - Chr1:96527852-96527871 MsG0180005814.01.T01:CDS 45.0%
ATTGGAGGAAACACGGATAG+AGG - Chr1:96527172-96527191 MsG0180005814.01.T01:intron 45.0%
CAGTGCTCAAGTAATCACGA+GGG + Chr1:96525500-96525519 None:intergenic 45.0%
CATCTCATCCATGTGCACTT+GGG - Chr1:96526015-96526034 MsG0180005814.01.T01:intron 45.0%
CATGGCCATAAACTGATGGT+TGG - Chr1:96526768-96526787 MsG0180005814.01.T01:intron 45.0%
CGGATAGAGGGGAATAATGA+TGG - Chr1:96527185-96527204 MsG0180005814.01.T01:intron 45.0%
CTGCAGATCACAACAAATGC+AGG + Chr1:96527502-96527521 None:intergenic 45.0%
CTGGAAGTGATCCATTGTGA+TGG - Chr1:96525658-96525677 MsG0180005814.01.T01:intron 45.0%
GAGGGGTCAATCTAATCCTA+TGG + Chr1:96525482-96525501 None:intergenic 45.0%
GTCTTAACAGTTGCGCACAA+AGG + Chr1:96525868-96525887 None:intergenic 45.0%
TCAGTTTATGGCCATGCGTT+TGG + Chr1:96526764-96526783 None:intergenic 45.0%
TCTCATTCCCCAATGGTGTA+GGG - Chr1:96526205-96526224 MsG0180005814.01.T01:intron 45.0%
TGCACATGGATGAGATGGTA+AGG + Chr1:96526012-96526031 None:intergenic 45.0%
TTGGAGGAAACACGGATAGA+GGG - Chr1:96527173-96527192 MsG0180005814.01.T01:intron 45.0%
TTTGAATGGTGTGTGTGTGG+TGG - Chr1:96526144-96526163 MsG0180005814.01.T01:intron 45.0%
! AGAGAAAGTGTGAGATACCG+TGG - Chr1:96526234-96526253 MsG0180005814.01.T01:intron 45.0%
! GGTAGGTATGGTGCTTTTCT+GGG - Chr1:96525690-96525709 MsG0180005814.01.T01:intron 45.0%
! GTACCTTGATAATTCTGCGG+AGG + Chr1:96525346-96525365 None:intergenic 45.0%
! TGGTAGGTATGGTGCTTTTC+TGG - Chr1:96525689-96525708 MsG0180005814.01.T01:intron 45.0%
! TGTGATCTGCAGCCTTCTTT+TGG - Chr1:96527510-96527529 MsG0180005814.01.T01:intron 45.0%
! TTGGTCAGTGACGTTTGTTG+AGG - Chr1:96526998-96527017 MsG0180005814.01.T01:intron 45.0%
AAGAGTGGAATTGGGTCGAG+AGG + Chr1:96525315-96525334 None:intergenic 50.0%
ACACAACCAAAGGGGTGACT+GGG + Chr1:96526809-96526828 None:intergenic 50.0%
AGTTGCGCACAAAGGTTACC+CGG + Chr1:96525860-96525879 None:intergenic 50.0%
ATAGACTGCCCAAGTGCACA+TGG + Chr1:96526026-96526045 None:intergenic 50.0%
ATGTTAGCAGATGCCCCTAG+GGG + Chr1:96527868-96527887 None:intergenic 50.0%
CCAAGTGCACATGGATGAGA+TGG + Chr1:96526017-96526036 None:intergenic 50.0%
CCATCTCATCCATGTGCACT+TGG - Chr1:96526014-96526033 MsG0180005814.01.T01:intron 50.0%
CCATGCTCTCTCCATCACAA+TGG + Chr1:96525672-96525691 None:intergenic 50.0%
CTGGGTTTGAATCCAGCCAT+GGG + Chr1:96525963-96525982 None:intergenic 50.0%
GAGCGTCCTTTCGAAACTGT+TGG + Chr1:96525269-96525288 None:intergenic 50.0%
GTCTCATTCCCCAATGGTGT+AGG - Chr1:96526204-96526223 MsG0180005814.01.T01:intron 50.0%
GTTTATGGCCATGCGTTTGG+TGG + Chr1:96526761-96526780 None:intergenic 50.0%
TACACAACCAAAGGGGTGAC+TGG + Chr1:96526810-96526829 None:intergenic 50.0%
TGGAGGAAACACGGATAGAG+GGG - Chr1:96527174-96527193 MsG0180005814.01.T01:intron 50.0%
TTAACACAGCTGCATGCCCA+TGG - Chr1:96525944-96525963 MsG0180005814.01.T01:intron 50.0%
! AATGGTGTGTGTGTGGTGGA+TGG - Chr1:96526148-96526167 MsG0180005814.01.T01:intron 50.0%
! CCATTGTGATGGAGAGAGCA+TGG - Chr1:96525669-96525688 MsG0180005814.01.T01:intron 50.0%
! GTCAGTGACGTTTGTTGAGG+AGG - Chr1:96527001-96527020 MsG0180005814.01.T01:intron 50.0%
! TGGAGAGAGCATGGTAGGTA+TGG - Chr1:96525678-96525697 MsG0180005814.01.T01:intron 50.0%
! TGTGATGGAGAGAGCATGGT+AGG - Chr1:96525673-96525692 MsG0180005814.01.T01:intron 50.0%
! TTAAGACTAGTCCCTCGAGC+CGG - Chr1:96525881-96525900 MsG0180005814.01.T01:intron 50.0%
!! ATCGCGTGCCCATTGATTCT+TGG + Chr1:96527476-96527495 None:intergenic 50.0%
!! CTCATGCATTTTGGCGGCAA+TGG - Chr1:96527569-96527588 MsG0180005814.01.T01:intron 50.0%
CCAGTTTAACCAGGGGTCCT+GGG + Chr1:96525981-96526000 None:intergenic 55.0%
CCATGGCTGGATTCAAACCC+AGG - Chr1:96525961-96525980 MsG0180005814.01.T01:intron 55.0%
CCTGGGTTTGAATCCAGCCA+TGG + Chr1:96525964-96525983 None:intergenic 55.0%
GCGGAGGTAAGGAAGAAGAG+TGG + Chr1:96525330-96525349 None:intergenic 55.0%
GGGTCGAGAGGAAGATGAAG+AGG + Chr1:96525303-96525322 None:intergenic 55.0%
TACTCACCCAGTCACCCCTT+TGG - Chr1:96526800-96526819 MsG0180005814.01.T01:intron 55.0%
TCCAGTTTAACCAGGGGTCC+TGG + Chr1:96525982-96526001 None:intergenic 55.0%
! TAAGACTAGTCCCTCGAGCC+GGG - Chr1:96525882-96525901 MsG0180005814.01.T01:intron 55.0%
CCCAGGACCCCTGGTTAAAC+TGG - Chr1:96525978-96525997 MsG0180005814.01.T01:intron 60.0%
GGATTCAAACCCAGGACCCC+TGG - Chr1:96525969-96525988 MsG0180005814.01.T01:intron 60.0%
TCACCCGTTGCAGTCCTACC+CGG + Chr1:96525903-96525922 None:intergenic 60.0%
ACTAGTCCCTCGAGCCGGGT+AGG - Chr1:96525886-96525905 MsG0180005814.01.T01:intron 65.0%
CACAGCTGCATGCCCATGGC+TGG - Chr1:96525948-96525967 MsG0180005814.01.T01:intron 65.0%
CGAGCCGGGTAGGACTGCAA+CGG - Chr1:96525896-96525915 MsG0180005814.01.T01:intron 65.0%
GAGCCGGGTAGGACTGCAAC+GGG - Chr1:96525897-96525916 MsG0180005814.01.T01:intron 65.0%
! TGCAGTCCTACCCGGCTCGA+GGG + Chr1:96525895-96525914 None:intergenic 65.0%
! TTGCAGTCCTACCCGGCTCG+AGG + Chr1:96525896-96525915 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 96525246 96528021 96525246 ID=MsG0180005814.01;Name=MsG0180005814.01
Chr1 mRNA 96525246 96528021 96525246 ID=MsG0180005814.01.T01;Parent=MsG0180005814.01;Name=MsG0180005814.01.T01;_AED=0.49;_eAED=0.81;_QI=0|0.5|0.2|1|0|0|5|0|256
Chr1 exon 96527729 96528021 96527729 ID=MsG0180005814.01.T01:exon:10620;Parent=MsG0180005814.01.T01
Chr1 exon 96526535 96526698 96526535 ID=MsG0180005814.01.T01:exon:10619;Parent=MsG0180005814.01.T01
Chr1 exon 96526334 96526456 96526334 ID=MsG0180005814.01.T01:exon:10618;Parent=MsG0180005814.01.T01
Chr1 exon 96525707 96525797 96525707 ID=MsG0180005814.01.T01:exon:10617;Parent=MsG0180005814.01.T01
Chr1 exon 96525246 96525345 96525246 ID=MsG0180005814.01.T01:exon:10616;Parent=MsG0180005814.01.T01
Chr1 CDS 96527729 96528021 96527729 ID=MsG0180005814.01.T01:cds;Parent=MsG0180005814.01.T01
Chr1 CDS 96526535 96526698 96526535 ID=MsG0180005814.01.T01:cds;Parent=MsG0180005814.01.T01
Chr1 CDS 96526334 96526456 96526334 ID=MsG0180005814.01.T01:cds;Parent=MsG0180005814.01.T01
Chr1 CDS 96525707 96525797 96525707 ID=MsG0180005814.01.T01:cds;Parent=MsG0180005814.01.T01
Chr1 CDS 96525246 96525345 96525246 ID=MsG0180005814.01.T01:cds;Parent=MsG0180005814.01.T01
Gene Sequence

>MsG0180005814.01.T01

TTGAACGAGCAAAGATGTTACCAACAGTTTCGAAAGGACGCTCTTCTTCTTCTTCCTCTTCATCTTCCTCTCGACCCAATTCCACTCTTCTTCCTTACCTCCGCAGAATTATCAAGGTACCCAACTGATCTCTTCATTTCTTCATCTTCTTCCCTTAATTTCGCTAATTCACTTCAAGATCCGATTCCCCATTTCATTGCAAATGCTGTTGAATTGTGGCGCAATATTATAAACCATAGGATTAGATTGACCCCTCGTGATTACTTGAGCACTGTGAAACTCAGAACCGCTCATGGCAACAAATGGATGTTGAATACACTTTTTGGCAAATGCTTCATCTATGTACGTCGCCAAAAGTTGTGTAAGTATATAGAAATCGTCTTCTGTTCCATCTTTTTTTTAACATCAAGAGTCAAGACTCACATTGTTACCATGATCTCATGCAACAACAATATGATCACCCCTTTGGTTGTGTATGTGTTTGGTGTTTTGAGTGACTGCTTCTCTGATTGCGTTTTTCGTACACTTGATATGGAACTGTGCAATGGATGCAGCTATCAGCATACAAAATATCACAAACAAACCAAGAATCAATGGGCACGCGATGACCCTGCATTTGTTGTGATCTGCAGCCTTCTTTTGGCTGTTGCAACTTTGGCATATTGTGCAGCGTATGACCATAGTTCTGGCCATGCACTTTTTGTTGTCTTCTCAGTGTTGCTATTCCATTTTCTATTGACTGGCATATTTCTGGCAACTTTTTGTTGGTGA
Protein sequence

>MsG0180005814.01.T01

LNEQRCYQQFRKDALLLLPLHLPLDPIPLFFLTSAELSRYPTDLFISSSSSLNFANSLQDPIPHFIANAVELWRNIINHRIRLTPRDYLSTVKLRTAHGNKWMLNTLFGKCFIYVRRQKLCKYIEIVFCSIFFLTSRVKTHIVTMISCNNNMITPLVVYVFGVLSDCFSDCVFRTLDMELCNGCSYQHTKYHKQTKNQWARDDPAFVVICSLLLAVATLAYCAAYDHSSGHALFVVFSVLLFHFLLTGIFLATFCW*