AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018337.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018337.01.T01 MTR_4g010140 70.370 162 39 5 17 171 121 280 1.53e-67 207
MsG0480018337.01.T01 MTR_4g010140 54.762 84 34 3 17 98 121 202 3.70e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018337.01.T01 AT3G15840 54.762 168 66 7 11 171 101 265 3.44e-48 157
MsG0480018337.01.T01 AT3G15840 54.762 168 66 7 11 171 104 268 3.61e-48 157
MsG0480018337.01.T01 AT3G15840 54.762 168 66 7 11 171 84 248 3.75e-48 157
MsG0480018337.01.T01 AT3G15840 54.762 168 66 7 11 171 50 214 3.90e-48 155
MsG0480018337.01.T01 AT3G15840 53.571 168 68 7 11 171 104 268 1.97e-46 153

Find 44 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCACCACCGCACATAAT+TGG 0.252908 4:+3504762 None:intergenic
GGCTTGTTCTGGTAAGGTTT+TGG 0.256890 4:+3504384 None:intergenic
TGGACTTGATTGCCAGTATA+TGG 0.267356 4:+3504404 None:intergenic
ATACTAATGGGAAGAAATTA+AGG 0.283709 4:-3503384 MsG0480018337.01.T01:three_prime_UTR
AGAATTCAATTGGCTTGTTC+TGG 0.295407 4:+3504373 None:intergenic
CTTCCCATTAGTATAGTAGA+AGG 0.305852 4:+3503393 None:intergenic
CATATTTGAATGCTGTATAT+AGG 0.316965 4:+3504700 None:intergenic
TGCGAACTGAATCTTGTTCC+AGG 0.318141 4:-3503645 MsG0480018337.01.T01:CDS
AATTTGTTAGTACGTAAATA+AGG 0.336841 4:-3503481 MsG0480018337.01.T01:three_prime_UTR
TGTCCCTTCTACTATACTAA+TGG 0.345634 4:-3503397 MsG0480018337.01.T01:three_prime_UTR
ATATTTGAATGCTGTATATA+GGG 0.361838 4:+3504701 None:intergenic
AGTAACATAGTACCATGTTT+AGG 0.374925 4:+3504673 None:intergenic
GATTGGGATGGTCCATATAC+TGG 0.402469 4:-3504416 MsG0480018337.01.T01:CDS
ACCTCGTTAAAGAATTCAAT+TGG 0.407330 4:+3504363 None:intergenic
GAATCAGAATCCACTTCAAT+TGG 0.409503 4:+3503561 None:intergenic
TCTATAACAGCAGTTGTGTC+TGG 0.416558 4:+3503907 None:intergenic
TCAATTGGCTTGTTCTGGTA+AGG 0.448143 4:+3504378 None:intergenic
TCCTTTCTGAGCATAGCTCT+TGG 0.448560 4:+3504741 None:intergenic
GTCCCTTCTACTATACTAAT+GGG 0.455766 4:-3503396 MsG0480018337.01.T01:three_prime_UTR
TGGGAAGAAATTAAGGTTCA+AGG 0.471518 4:-3503377 MsG0480018337.01.T01:three_prime_UTR
GGATCATACAAGGGCGAGCT+AGG 0.494764 4:+3503612 None:intergenic
TAGTAGTTAGCACTGAACTT+TGG 0.523402 4:-3503426 MsG0480018337.01.T01:three_prime_UTR
CTCGCCCTTGTATGATCCAA+TGG 0.534387 4:-3503607 MsG0480018337.01.T01:CDS
CCATCATCCACATTAGCCAT+TGG 0.540095 4:+3503591 None:intergenic
CCAATGGCTAATGTGGATGA+TGG 0.545754 4:-3503591 MsG0480018337.01.T01:CDS
ATTAGCCATTGGATCATACA+AGG 0.545804 4:+3503602 None:intergenic
TTTGTTGTAGGGCTTGGCAG+AGG 0.558067 4:-3503965 MsG0480018337.01.T01:intron
CAAATATGTGTTCCTAAACA+TGG 0.562988 4:-3504685 MsG0480018337.01.T01:CDS
AATTACAACAGCATGCATGC+TGG 0.565034 4:+3503500 None:intergenic
TTCCCATTAGTATAGTAGAA+GGG 0.567662 4:+3503394 None:intergenic
CTATGTTACTCAATTTCTAA+CGG 0.571229 4:-3504660 MsG0480018337.01.T01:CDS
GCTATGCTCAGAAAGGATAG+AGG 0.573110 4:-3504735 MsG0480018337.01.T01:CDS
GCGAACTGAATCTTGTTCCA+GGG 0.581743 4:-3503644 MsG0480018337.01.T01:CDS
ACCAAGAGCTATGCTCAGAA+AGG 0.582588 4:-3504742 MsG0480018337.01.T01:CDS
AATCAACCAATTATGTGCGG+TGG 0.594257 4:-3504768 MsG0480018337.01.T01:CDS
TTGGCAGAGGAACTAAGTAA+AGG 0.600606 4:-3503952 MsG0480018337.01.T01:CDS
GTATGATCCAATGGCTAATG+TGG 0.607801 4:-3503598 MsG0480018337.01.T01:CDS
TCAGTGCTAACTACTAAGTG+TGG 0.616075 4:+3503433 None:intergenic
ACAAATGGAGTAGATTGGGA+TGG 0.618203 4:-3504428 MsG0480018337.01.T01:intron
TGGATCATGTCCAATTGAAG+TGG 0.628823 4:-3503571 MsG0480018337.01.T01:CDS
TTTAATCAACCAATTATGTG+CGG 0.634307 4:-3504771 MsG0480018337.01.T01:CDS
GAGCTAGGATCGATGCACCC+TGG 0.654050 4:+3503627 None:intergenic
GCCAATTGAATTCTTTAACG+AGG 0.659443 4:-3504364 MsG0480018337.01.T01:intron
TTAGCCATTGGATCATACAA+GGG 0.708949 4:+3503603 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTCTCTTTTCATA+AGG - Chr4:3504390-3504409 MsG0480018337.01.T01:CDS 15.0%
!!! TATTTTTTTTATGATCAAGA+GGG + Chr4:3504196-3504215 None:intergenic 15.0%
!!! TTATTTTTTTTATGATCAAG+AGG + Chr4:3504197-3504216 None:intergenic 15.0%
!! AATTACATTGGACAAAATTT+CGG - Chr4:3503666-3503685 MsG0480018337.01.T01:intron 20.0%
!! AGATATTTCTTGAACAAAAA+TGG - Chr4:3503989-3504008 MsG0480018337.01.T01:intron 20.0%
!! ATATTTGAATGCTGTATATA+GGG + Chr4:3503514-3503533 None:intergenic 20.0%
!! TTAAATCATTGTAATAGAGT+TGG - Chr4:3504502-3504521 MsG0480018337.01.T01:intron 20.0%
!! TTATAGAAAAATGCTATGAT+TGG - Chr4:3504321-3504340 MsG0480018337.01.T01:intron 20.0%
!!! AATTTGTTAGTACGTAAATA+AGG - Chr4:3504731-3504750 MsG0480018337.01.T01:CDS 20.0%
!!! AGTTTTAATTTTGCATGTTT+AGG - Chr4:3503414-3503433 MsG0480018337.01.T01:three_prime_UTR 20.0%
!!! ATGTATTATTCTTTGTTGTA+GGG - Chr4:3504236-3504255 MsG0480018337.01.T01:intron 20.0%
!!! ATTGGTAATTTATCAAAAGA+AGG - Chr4:3504339-3504358 MsG0480018337.01.T01:intron 20.0%
!!! ATTTTGTCCAATGTAATTTT+AGG + Chr4:3503664-3503683 None:intergenic 20.0%
!!! ATTTTTTTTATGATCAAGAG+GGG + Chr4:3504195-3504214 None:intergenic 20.0%
!!! TTGATTTTCTCATTTACAAA+TGG - Chr4:3503769-3503788 MsG0480018337.01.T01:intron 20.0%
!!! TTGGTAATTTATCAAAAGAA+GGG - Chr4:3504340-3504359 MsG0480018337.01.T01:intron 20.0%
! ATACTAATGGGAAGAAATTA+AGG - Chr4:3504828-3504847 MsG0480018337.01.T01:CDS 25.0%
! ATTTAAAGAGTAACTACAAG+TGG + Chr4:3504488-3504507 None:intergenic 25.0%
! ATTTACAAATGGAGTAGATT+GGG - Chr4:3503780-3503799 MsG0480018337.01.T01:intron 25.0%
! CATATTTGAATGCTGTATAT+AGG + Chr4:3503515-3503534 None:intergenic 25.0%
! CTATGTTACTCAATTTCTAA+CGG - Chr4:3503552-3503571 MsG0480018337.01.T01:CDS 25.0%
! GTTATAACATGAAGATTAGA+TGG - Chr4:3504049-3504068 MsG0480018337.01.T01:intron 25.0%
! TGGTAATTTATCAAAAGAAG+GGG - Chr4:3504341-3504360 MsG0480018337.01.T01:intron 25.0%
! TTTAATCAACCAATTATGTG+CGG - Chr4:3503441-3503460 MsG0480018337.01.T01:three_prime_UTR 25.0%
!! AGATTAGATGGTTTAGATTT+TGG - Chr4:3504061-3504080 MsG0480018337.01.T01:intron 25.0%
!! CATGTATTATTCTTTGTTGT+AGG - Chr4:3504235-3504254 MsG0480018337.01.T01:intron 25.0%
!!! GTTTTGACCTAAAATTACAT+TGG - Chr4:3503654-3503673 MsG0480018337.01.T01:CDS 25.0%
!!! TTTAATTTTGCATGTTTAGG+TGG - Chr4:3503417-3503436 MsG0480018337.01.T01:three_prime_UTR 25.0%
ACCTCGTTAAAGAATTCAAT+TGG + Chr4:3503852-3503871 None:intergenic 30.0%
AGTAACATAGTACCATGTTT+AGG + Chr4:3503542-3503561 None:intergenic 30.0%
ATTAAGTGACACTGTAATTG+TGG + Chr4:3503614-3503633 None:intergenic 30.0%
CAAATATGTGTTCCTAAACA+TGG - Chr4:3503527-3503546 MsG0480018337.01.T01:three_prime_UTR 30.0%
CATTTACAAATGGAGTAGAT+TGG - Chr4:3503779-3503798 MsG0480018337.01.T01:intron 30.0%
GCCATTAGATGAAGATTAAA+CGG + Chr4:3504127-3504146 None:intergenic 30.0%
GTTTAATCTTCATCTAATGG+CGG - Chr4:3504126-3504145 MsG0480018337.01.T01:intron 30.0%
TTCCCATTAGTATAGTAGAA+GGG + Chr4:3504821-3504840 None:intergenic 30.0%
! ATGGTCGAAAAAATCTTTTC+AGG - Chr4:3504536-3504555 MsG0480018337.01.T01:intron 30.0%
! TGGTCGAAAAAATCTTTTCA+GGG - Chr4:3504537-3504556 MsG0480018337.01.T01:intron 30.0%
ATGTTGAATTGACAGTGAGT+TGG - Chr4:3503588-3503607 MsG0480018337.01.T01:CDS 35.0%
ATTAGCCATTGGATCATACA+AGG + Chr4:3504613-3504632 None:intergenic 35.0%
CTTCCCATTAGTATAGTAGA+AGG + Chr4:3504822-3504841 None:intergenic 35.0%
GAATCAGAATCCACTTCAAT+TGG + Chr4:3504654-3504673 None:intergenic 35.0%
GCCAATTGAATTCTTTAACG+AGG - Chr4:3503848-3503867 MsG0480018337.01.T01:intron 35.0%
GCCGTTTAATCTTCATCTAA+TGG - Chr4:3504123-3504142 MsG0480018337.01.T01:intron 35.0%
GTCCCTTCTACTATACTAAT+GGG - Chr4:3504816-3504835 MsG0480018337.01.T01:CDS 35.0%
GTTATAACTGAGATTGCAAG+TGG + Chr4:3504037-3504056 None:intergenic 35.0%
TACACTATTTCACGACTACA+AGG - Chr4:3504161-3504180 MsG0480018337.01.T01:intron 35.0%
TGGGAAGAAATTAAGGTTCA+AGG - Chr4:3504835-3504854 MsG0480018337.01.T01:CDS 35.0%
TGTCCCTTCTACTATACTAA+TGG - Chr4:3504815-3504834 MsG0480018337.01.T01:CDS 35.0%
TTAGCCATTGGATCATACAA+GGG + Chr4:3504612-3504631 None:intergenic 35.0%
! TAGTAGTTAGCACTGAACTT+TGG - Chr4:3504786-3504805 MsG0480018337.01.T01:CDS 35.0%
! TTATTCTTTGTTGTAGGGCT+TGG - Chr4:3504241-3504260 MsG0480018337.01.T01:intron 35.0%
!! AGAATTCAATTGGCTTGTTC+TGG + Chr4:3503842-3503861 None:intergenic 35.0%
!!! AGAGTTGGTTTTCTAAACGA+TGG - Chr4:3504517-3504536 MsG0480018337.01.T01:intron 35.0%
AATCAACCAATTATGTGCGG+TGG - Chr4:3503444-3503463 MsG0480018337.01.T01:three_prime_UTR 40.0%
AATTACAACAGCATGCATGC+TGG + Chr4:3504715-3504734 None:intergenic 40.0%
ACAAATGGAGTAGATTGGGA+TGG - Chr4:3503784-3503803 MsG0480018337.01.T01:intron 40.0%
GTATGATCCAATGGCTAATG+TGG - Chr4:3504614-3504633 MsG0480018337.01.T01:intron 40.0%
TCAGTGCTAACTACTAAGTG+TGG + Chr4:3504782-3504801 None:intergenic 40.0%
TCTATAACAGCAGTTGTGTC+TGG + Chr4:3504308-3504327 None:intergenic 40.0%
TGGACTTGATTGCCAGTATA+TGG + Chr4:3503811-3503830 None:intergenic 40.0%
TGGATCATGTCCAATTGAAG+TGG - Chr4:3504641-3504660 MsG0480018337.01.T01:intron 40.0%
TTGGCAGAGGAACTAAGTAA+AGG - Chr4:3504260-3504279 MsG0480018337.01.T01:intron 40.0%
!! TCAATTGGCTTGTTCTGGTA+AGG + Chr4:3503837-3503856 None:intergenic 40.0%
ACCAAGAGCTATGCTCAGAA+AGG - Chr4:3503470-3503489 MsG0480018337.01.T01:three_prime_UTR 45.0%
CCAATGGCTAATGTGGATGA+TGG - Chr4:3504621-3504640 MsG0480018337.01.T01:intron 45.0%
CCATCATCCACATTAGCCAT+TGG + Chr4:3504624-3504643 None:intergenic 45.0%
GATTGGGATGGTCCATATAC+TGG - Chr4:3503796-3503815 MsG0480018337.01.T01:intron 45.0%
GCGAACTGAATCTTGTTCCA+GGG - Chr4:3504568-3504587 MsG0480018337.01.T01:intron 45.0%
GCTATGCTCAGAAAGGATAG+AGG - Chr4:3503477-3503496 MsG0480018337.01.T01:three_prime_UTR 45.0%
TCCTTTCTGAGCATAGCTCT+TGG + Chr4:3503474-3503493 None:intergenic 45.0%
TGCGAACTGAATCTTGTTCC+AGG - Chr4:3504567-3504586 MsG0480018337.01.T01:intron 45.0%
!! GGCTTGTTCTGGTAAGGTTT+TGG + Chr4:3503831-3503850 None:intergenic 45.0%
!! AATAAATAATAATAACTTAA+TGG - Chr4:3504212-3504231 MsG0480018337.01.T01:intron 5.0%
CTCGCCCTTGTATGATCCAA+TGG - Chr4:3504605-3504624 MsG0480018337.01.T01:intron 50.0%
GGTTCACCACCGCACATAAT+TGG + Chr4:3503453-3503472 None:intergenic 50.0%
! TTTGTTGTAGGGCTTGGCAG+AGG - Chr4:3504247-3504266 MsG0480018337.01.T01:intron 50.0%
GGATCATACAAGGGCGAGCT+AGG + Chr4:3504603-3504622 None:intergenic 55.0%
GAGCTAGGATCGATGCACCC+TGG + Chr4:3504588-3504607 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 3503369 3504865 3503369 ID=MsG0480018337.01;Name=MsG0480018337.01
Chr4 mRNA 3503369 3504865 3503369 ID=MsG0480018337.01.T01;Parent=MsG0480018337.01;Name=MsG0480018337.01.T01;_AED=0.44;_eAED=0.49;_QI=0|0.25|0.2|1|0|0|5|182|171
Chr4 exon 3503369 3503676 3503369 ID=MsG0480018337.01.T01:exon:1008;Parent=MsG0480018337.01.T01
Chr4 exon 3503813 3503822 3503813 ID=MsG0480018337.01.T01:exon:1007;Parent=MsG0480018337.01.T01
Chr4 exon 3503892 3503977 3503892 ID=MsG0480018337.01.T01:exon:1006;Parent=MsG0480018337.01.T01
Chr4 exon 3504365 3504437 3504365 ID=MsG0480018337.01.T01:exon:1005;Parent=MsG0480018337.01.T01
Chr4 exon 3504645 3504865 3504645 ID=MsG0480018337.01.T01:exon:1004;Parent=MsG0480018337.01.T01
Chr4 CDS 3504645 3504865 3504645 ID=MsG0480018337.01.T01:cds;Parent=MsG0480018337.01.T01
Chr4 CDS 3504365 3504437 3504365 ID=MsG0480018337.01.T01:cds;Parent=MsG0480018337.01.T01
Chr4 CDS 3503892 3503977 3503892 ID=MsG0480018337.01.T01:cds;Parent=MsG0480018337.01.T01
Chr4 CDS 3503813 3503822 3503813 ID=MsG0480018337.01.T01:cds;Parent=MsG0480018337.01.T01
Chr4 CDS 3503551 3503676 3503551 ID=MsG0480018337.01.T01:cds;Parent=MsG0480018337.01.T01
Chr4 three_prime_UTR 3503369 3503550 3503369 ID=MsG0480018337.01.T01:three_prime_utr;Parent=MsG0480018337.01.T01
Gene Sequence

>MsG0480018337.01.T01

ATGTTAGTGTTTTGTCTTCATTTTGCTGTTATTTTTCTCAAATTGAGTTTTAATTTTGCATGTTTAGGTGGTTTTAATCAACCAATTATGTGCGGTGGTGAACCAAGAGCTATGCTCAGAAAGGATAGAGGCAAAGCTGATTCCCTATATACAGCATTCAAATATGTGTTCCTAAACATGGTACTATGTTACTCAATTTCTAACGGTATGTCATTCAGTATATTGGGATGGTCCATATACTGGCAATCAAGTCCAAAACCTTACCAGAACAAGCCAATTGAATTCTTTAACGAGGGCTTGGCAGAGGAACTAAGTAAAGGAGCATGTGAACAAGCAATATTTCCAGACACAACTGCTGTTATAGAAAAATGCTATGATTGGAAACATTATGGTGATCGCTGCGAACTGAATCTTGTTCCAGGGTGCATCGATCCTAGCTCGCCCTTGTATGATCCAATGGCTAATGTGGATGATGGATCATGTCCAATTGAAGTGGATTCTGATTCTGATGAATAA

Protein sequence

>MsG0480018337.01.T01

MLVFCLHFAVIFLKLSFNFACLGGFNQPIMCGGEPRAMLRKDRGKADSLYTAFKYVFLNMVLCYSISNGMSFSILGWSIYWQSSPKPYQNKPIEFFNEGLAEELSKGACEQAIFPDTTAVIEKCYDWKHYGDRCELNLVPGCIDPSSPLYDPMANVDDGSCPIEVDSDSDE*