AlfalfaGEDB | MsG0480021300.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021300.01

KEGG: K05236
GO: GO:0005198 GO:0005515 GO:0006886 GO:0016192 GO:0030117 GO:0030126
PFAM: PF00400 PF04053 PF06957

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021300.01.T01	XP_024637924.2	99.673	1223	4	0	1	1223	1	1223	0	2543

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021300.01.T01	Q94A40	83.02	1225	197	6	1	1223	1	1216	0	2137

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021300.01.T01	A0A396I9C2	99.673	1223	4	0	1	1223	1	1223	0.0	2543

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049008.01	MsG0480021300.01	0.814167	1.768262e-51	1.177767e-48
MsG0180000535.01	MsG0480021300.01	0.862674	4.287669e-64	1.265956e-60
MsG0180003637.01	MsG0480021300.01	0.866748	2.272076e-65	7.743076e-62
MsG0180004121.01	MsG0480021300.01	0.813754	2.180902e-51	1.436454e-48
MsG0180004705.01	MsG0480021300.01	0.824643	7.221611e-54	6.435238e-51
MsG0180005000.01	MsG0480021300.01	0.867217	1.610711e-65	5.579306e-62
MsG0180005367.01	MsG0480021300.01	0.897330	1.560646e-76	1.754713e-72
MsG0180005814.01	MsG0480021300.01	0.802555	5.307222e-49	2.607391e-46
MsG0180005898.01	MsG0480021300.01	-0.805040	1.617409e-49	8.469762e-47
MsG0180005906.01	MsG0480021300.01	0.814715	1.337798e-51	9.043900e-49

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PPI

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Gene1	Gene2	Type
MsG0280011152.01	MsG0480021300.01	PPI
MsG0280011152.01	MsG0480021300.01	PPI
MsG0480021241.01	MsG0480021300.01	PPI
MsG0480021300.01	MsG0280011152.01	PPI
MsG0880042615.01	MsG0480021300.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021300.01.T01	MTR_4g071130	98.283	1223	4	1	1	1223	1	1206	0.0	2499
MsG0480021300.01.T01	MTR_1g090827	88.072	1224	137	4	1	1223	1	1216	0.0	2274
MsG0480021300.01.T01	MTR_4g011950	25.550	818	515	25	4	782	10	772	1.01e-70	256
MsG0480021300.01.T01	MTR_2g102277	25.275	819	516	24	4	782	10	772	1.10e-68	250
MsG0480021300.01.T01	MTR_2g102277	25.275	819	516	24	4	782	10	772	1.62e-68	249
MsG0480021300.01.T01	MTR_2g102267	31.329	316	184	6	4	315	12	298	8.78e-47	171
MsG0480021300.01.T01	MTR_2g102267	29.518	166	113	3	2	163	94	259	5.69e-20	92.8
MsG0480021300.01.T01	MTR_4g119620	27.000	300	189	7	37	308	13	310	7.34e-28	116
MsG0480021300.01.T01	MTR_4g119620	26.596	282	176	8	2	279	71	325	5.24e-22	99.0
MsG0480021300.01.T01	MTR_1g072140	28.929	280	174	7	50	308	26	301	4.04e-26	110

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021300.01.T01	AT1G62020	83.020	1225	197	6	1	1223	1	1216	0.0	2137
MsG0480021300.01.T01	AT2G21390	82.219	1226	207	7	1	1223	1	1218	0.0	2120
MsG0480021300.01.T01	AT3G15980	26.707	820	503	27	4	782	10	772	8.89e-73	262
MsG0480021300.01.T01	AT3G15980	26.707	820	503	27	4	782	10	772	1.24e-72	261
MsG0480021300.01.T01	AT3G15980	26.707	820	503	27	4	782	10	772	1.43e-72	261
MsG0480021300.01.T01	AT3G15980	26.707	820	503	27	4	782	10	772	1.43e-72	261
MsG0480021300.01.T01	AT3G15980	26.707	820	503	27	4	782	10	772	1.43e-72	261
MsG0480021300.01.T01	AT3G15980	26.707	820	503	27	4	782	10	772	1.43e-72	261
MsG0480021300.01.T01	AT1G52360	26.220	820	507	27	4	782	10	772	2.01e-70	255
MsG0480021300.01.T01	AT1G52360	26.220	820	507	27	4	782	10	772	2.39e-70	255

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Find 341 sgRNAs with CRISPR-Local

Find 869 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAAGCTAAGACAGCA+AGG	0.652934	4:-56442882	MsG0480021300.01.T01:CDS
AAAAGTTCAATCAACGAATC+TGG	0.306067	4:+56442623	None:intergenic
AAAATCGGTCATGTTCCCTG+CGG	0.740048	4:+56445425	None:intergenic
AAACACGAACAGTCTGATCC+AGG	0.483052	4:+56446586	None:intergenic
AAACCATTCGTATCTGGAAT+TGG	0.402705	4:-56446704	MsG0480021300.01.T01:CDS
AAACCCATTTGTAATCTGTG+GGG	0.712530	4:-56442797	MsG0480021300.01.T01:CDS
AAACTTGCTCTGTAGGTCTG+AGG	0.640502	4:-56444879	MsG0480021300.01.T01:CDS
AAAGATCACTGGTACCGATT+GGG	0.496714	4:-56444251	MsG0480021300.01.T01:CDS
AAAGGCTTGCAGCTGAACTG+GGG	0.609705	4:-56443947	MsG0480021300.01.T01:CDS
AAAGTTCAATCAACGAATCT+GGG	0.536597	4:+56442624	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGTTCAATCAACGAATC+TGG	+	Chr4:56455232-56455251	None:intergenic	30.0%
AAAGTTCAATCAACGAATCT+GGG	+	Chr4:56455231-56455250	None:intergenic	30.0%
AACATATCTATCAACTAGCT+AGG	+	Chr4:56443816-56443835	None:intergenic	30.0%
AACCAAAACCTTAAGTCATT+AGG	+	Chr4:56446038-56446057	None:intergenic	30.0%
AAGAATTATACCCTACCAAA+CGG	-	Chr4:56446845-56446864	MsG0480021300.01.T01:intron	30.0%
AAGATGTAAAAGTGAAGAGA+TGG	+	Chr4:56449404-56449423	None:intergenic	30.0%
AATCGCAAAATATCATCTGA+AGG	+	Chr4:56451327-56451346	None:intergenic	30.0%
ACATATCTATCAACTAGCTA+GGG	+	Chr4:56443815-56443834	None:intergenic	30.0%
AGACACAAGTACTTACTATT+CGG	-	Chr4:56452618-56452637	MsG0480021300.01.T01:intron	30.0%
AGACTACATACTTGATGAAA+GGG	+	Chr4:56453054-56453073	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	56442331	56455543	56442331	ID=MsG0480021300.01;Name=MsG0480021300.01
Chr4	mRNA	56442331	56455543	56442331	ID=MsG0480021300.01.T01;Parent=MsG0480021300.01;Name=MsG0480021300.01.T01;_AED=0.48;_eAED=0.48;_QI=262\|1\|1\|1\|0.5\|0.4\|5\|302\|1223
Chr4	exon	56442331	56445601	56442331	ID=MsG0480021300.01.T01:exon:19125;Parent=MsG0480021300.01.T01
Chr4	exon	56446367	56446861	56446367	ID=MsG0480021300.01.T01:exon:19124;Parent=MsG0480021300.01.T01
Chr4	exon	56448866	56449081	56448866	ID=MsG0480021300.01.T01:exon:19123;Parent=MsG0480021300.01.T01
Chr4	exon	56449207	56449378	56449207	ID=MsG0480021300.01.T01:exon:19122;Parent=MsG0480021300.01.T01
Chr4	exon	56455462	56455543	56455462	ID=MsG0480021300.01.T01:exon:19121;Parent=MsG0480021300.01.T01
Chr4	five_prime_UTR	56455462	56455543	56455462	ID=MsG0480021300.01.T01:five_prime_utr;Parent=MsG0480021300.01.T01
Chr4	five_prime_UTR	56449207	56449378	56449207	ID=MsG0480021300.01.T01:five_prime_utr;Parent=MsG0480021300.01.T01
Chr4	five_prime_UTR	56449074	56449081	56449074	ID=MsG0480021300.01.T01:five_prime_utr;Parent=MsG0480021300.01.T01
Chr4	CDS	56448866	56449073	56448866	ID=MsG0480021300.01.T01:cds;Parent=MsG0480021300.01.T01
Chr4	CDS	56446367	56446861	56446367	ID=MsG0480021300.01.T01:cds;Parent=MsG0480021300.01.T01
Chr4	CDS	56442633	56445601	56442633	ID=MsG0480021300.01.T01:cds;Parent=MsG0480021300.01.T01
Chr4	three_prime_UTR	56442331	56442632	56442331	ID=MsG0480021300.01.T01:three_prime_utr;Parent=MsG0480021300.01.T01

Gene Sequence

>MsG0480021300.01.T01

ATGTTAACGAAATTCGAGACGAAAAGTAACAGAGTGAAGGGATTGAGTTTCCACAGCAAGAGACCATGGATACTTGCGAGTCTTCACAGTGGTGTGATCCAGCTATGGGACTATCGAATGGGGACCTTGATTGATCGATTCGATGAACATGATGGTCCTGTTCGTGGCGTTCATTTTCACAATTCTCAACCTCTCTTTGTATCTGGAGGGGATGATTACAAGATTAAGGTCTGGAACTATAAGATGCATAGGTGCTTATTCACTCTTCTAGGACACCTTGATTATATTCGTACAGTGCAATTTCATCATGAGAACCCATGGATTGTGAGTGCTAGTGATGATCAAACCATTCGTATCTGGAATTGGCAGTCACGTACCTGTATATCTGTTTTAACTGGGCACAACCATTATGTTATGTGTGCTTCATTCCATCCAAAAGAGGATATTGTCGTGTCAGCCTCCCTGGATCAGACTGTTCGTGTTTGGGATATTGGTTCTCTCAAAAGGAAGGCTGGACCTCCTTCAGATGATATTTTGCGATTGAGTCAGATGAACACTGATCTTTTTGGTGGCGTCGATGCAGTTGTTAAGTATGTGTTGGAAGGTCATGACCGGGGTGTCAACTGGGCTGCTTTTCATCCTACACTGCCTCTTATTGTCTCAGGAGCTGATGACCGTCAAGTGAAACTTTGGAGGATGAATGACACTAAGGCATGGGAAGTGGATACTTTGAGAGGGCACATGAATAATGTTTCATGTGTTATGTTCCATGCCAAACAGGACATCATTGTGTCAAATTCTGAGGATAAAAGTATTCGAGTATGGGATGCAACAAAGAGGACTGGTATTCAAACCTTCCGCAGGGAACATGACCGATTTTGGATTCTTTCAACACATCCTGAAATGAATCTGTTGGCAGCTGGTCATGATAGTGGCATGATAGTCTTCAAGCTGGAGAGAGAAAGGCCCGCCTTCGCTGTTAGTAATGATTCTTTGTTCTACACCAAAGACCGCTTTTTGCGTTTCTATGAATTTTCAACACAGAGAGAGACACAAGTACTTACTATTCGGCGACCTGGTTCTTTAAGCCTGAATCAAAGTCCTAAGACTCTTTCCTACAGTCCTACTGAAAATGCAATTCTTCTCTGCTCTGATGTGGACGGTGGATCTTATGAGTTGTACTGCATATCCAAGGATAGCACAATTAAGGACAGTTTTGGTAGGGGTGATATGCAAGAACCAAAGAAAGGTCTTGGAGGATCAGCAGTCTTTGTGGCTCGGAATAGGTTTGCCGTGCTTGAAAAAAGCAGCAACCAAGTCCTTTTAAAAAACCTGAAGAACGAGCTTGTTAAAAAGAGTGTGCTCCCCATTGCCACTGATGCTATATTTTATGCTGGAACAGGAAACTTGCTCTGTAGGTCTGAGGATAGGGTTTTTATATTTGATCTTCAGCAGAGGATTGTTCTTGGCGATCTTCAGACCCCTTTCATCAAGTATGTAGTCTGGTCTAATGAGAACAATGACAGTGTTGCTTTACTCAGCAAACATGCAATTGTAATTGCTAGCAAGAAGCTGGTGCATCAATGCACCCTCCACGAGACAATCCGTGTAAAAAGCGGAGCCTGGGATGACAATGGTATTTTTATTTACACAACATTAAATCATATCAAATACTGCCTTCCAAATGGAGACAGTGGGATAATAAAAACACTGGACGTCCCAATCTATATCACCAAGGTTGTTGGAAATACCATCTTCTGCTTGGGCCGGGATGGGAAAAACAAAGCTATAACCATTGATGCTACTGAGTATATCTTTAAGCTTTCCTTGTTGAAGAAAAGGTATGACCATGTTATGAACATGATAAAGAATTCACAACTTTGTGGACAAGCTATGATTGCTTATCTACAACAGAAAGGATTTCCTGAGGTTGCTCTCCATTTTGTGAAAGATGAGAGAATTCGGTTCAATTTGGCTATAGAGAGTGGGAACATTCAAATTGCAGTTGCATCAGCTACAGCAATTGATGAGAAAGATCACTGGTACCGATTGGGAGTTGAAGCTCTTCGACAAGGCAATGCTGGTATAGTGGAGTATGCATATCAGAGGACCAAGAATTTTGAAAGATTATCTTTCCTATATCTCATCACTGGTAATGTAGAGAAACTATCAAAGATGTTGAAAATTGCTGAAGTCAAGAATGATGTCATGGGCCAGTTTCACAATGCCTTATATATGGGTGATATTCGAGAGCGTGTTAAGATCTTGGAGAATGTGGGCCACTTGCCTCTTGCTTATATCACTGCATCAACCCATGGGCTACATGACGTTGCTGAAAGGCTTGCAGCTGAACTGGGGGACAACGTTCCATCTTTGCCCGAGGGGAAAGTACCATCTCTCTTGATACCTCCATCACCTGTACTGTGTTGTGGTGATTGGCCGCTCCTTAGGGTCATGCGAGGCATATTTGATGGTGGGTTTAGAAATGCTGATCAAGATGCTGACGAAGAAGAGTTTGATGATGCTCCTGAAGGTGATTGGGTTGAGGATATCGGCTTGGATGTTGATGGATTGGAAAATGGAGATGTTACTGCAATTTTGGATGATGGAGAGGCGGCTGAAGAAGGTGATGAAGAGGGTGGATGGGAGCTGGAAGATTTAGAGCTCCCCCCTGAAGCAGACACTCCAAAAGCTTCTGTCAGTACACGGTCTTCAGTTTTTGTGGCCCCAACACCTGGGATGGCAGTTAGCCAGATATGGATTCAGAGATCATCTCTTGCTGCTGATCATGCAGCTGCTGGCAATTTTGATACTGCAATGAGATTACTGAACAGGCAGCTTGGGATAAAAAACTTTGCCCCCTTGAGATCCATGTTCCTAGATCTTCATTCTGCCAGTCACTCGTATCTTCGCGCCTTTTCATCCGCTCCAGTTGTACCACTTGCTGTTGAAAGGGGTTGGACAGAGTCGTCTAGTCCAAATGTGAGAGGCCCACCGGCACTTCCTTACAAATTGTCTCAATTAGATGAAAAGCTCAAAGCTGGTTATAAGTCAACAACTGCTGGGAAATTCACTGAGGCTCTTCGGACATTTGCCAATATTCTCCATACAATCCCTTTGATTGTCGTTGAGTCAAGGAGGGAAGTGGATGATGTAAAGGAATTGATAATCATAGTCAAAGAGTATGTTCTGGCTCTGCAGATGGAGCTGAAAAGAAGGGAAATGAAGGACAATCCAGCACGCCAGCAGGAACTCGCAGCATATTTCACCCACTGCAATCTTCAGACACCTCACTTGAGGCTAGCTTTGTTGAATGCAATGACTGTCTGCTACAAAGCAAAGAACCTTGCCACAGCTGGTAACTTCGCCAGGAGACTACTCGAGACCAATCCTAGCATCGAAAACCAAGCTAAGACAGCAAGGCAAGTGCTGGCTGCTGCAGAAAGGAATATGACTGATGCCACGGAGTTGAACTATGATTTCCGAAACCCATTTGTAATCTGTGGGGCAACCTATGTGCCCATTTATCGTGGACAGAAGGACGTCTCTTGCCCATATTGTACTTCCCGTTTTGTACCAACCCAGGAGGGGCAGCTATGCACTGTGTGTGAACTTTCAGTTGTGGGGGCCGATGCTTCTGGATTGCTCTGTTCTCCTGCCCAGATTCGTTGA

Protein sequence

>MsG0480021300.01.T01

MLTKFETKSNRVKGLSFHSKRPWILASLHSGVIQLWDYRMGTLIDRFDEHDGPVRGVHFHNSQPLFVSGGDDYKIKVWNYKMHRCLFTLLGHLDYIRTVQFHHENPWIVSASDDQTIRIWNWQSRTCISVLTGHNHYVMCASFHPKEDIVVSASLDQTVRVWDIGSLKRKAGPPSDDILRLSQMNTDLFGGVDAVVKYVLEGHDRGVNWAAFHPTLPLIVSGADDRQVKLWRMNDTKAWEVDTLRGHMNNVSCVMFHAKQDIIVSNSEDKSIRVWDATKRTGIQTFRREHDRFWILSTHPEMNLLAAGHDSGMIVFKLERERPAFAVSNDSLFYTKDRFLRFYEFSTQRETQVLTIRRPGSLSLNQSPKTLSYSPTENAILLCSDVDGGSYELYCISKDSTIKDSFGRGDMQEPKKGLGGSAVFVARNRFAVLEKSSNQVLLKNLKNELVKKSVLPIATDAIFYAGTGNLLCRSEDRVFIFDLQQRIVLGDLQTPFIKYVVWSNENNDSVALLSKHAIVIASKKLVHQCTLHETIRVKSGAWDDNGIFIYTTLNHIKYCLPNGDSGIIKTLDVPIYITKVVGNTIFCLGRDGKNKAITIDATEYIFKLSLLKKRYDHVMNMIKNSQLCGQAMIAYLQQKGFPEVALHFVKDERIRFNLAIESGNIQIAVASATAIDEKDHWYRLGVEALRQGNAGIVEYAYQRTKNFERLSFLYLITGNVEKLSKMLKIAEVKNDVMGQFHNALYMGDIRERVKILENVGHLPLAYITASTHGLHDVAERLAAELGDNVPSLPEGKVPSLLIPPSPVLCCGDWPLLRVMRGIFDGGFRNADQDADEEEFDDAPEGDWVEDIGLDVDGLENGDVTAILDDGEAAEEGDEEGGWELEDLELPPEADTPKASVSTRSSVFVAPTPGMAVSQIWIQRSSLAADHAAAGNFDTAMRLLNRQLGIKNFAPLRSMFLDLHSASHSYLRAFSSAPVVPLAVERGWTESSSPNVRGPPALPYKLSQLDEKLKAGYKSTTAGKFTEALRTFANILHTIPLIVVESRREVDDVKELIIIVKEYVLALQMELKRREMKDNPARQQELAAYFTHCNLQTPHLRLALLNAMTVCYKAKNLATAGNFARRLLETNPSIENQAKTARQVLAAAERNMTDATELNYDFRNPFVICGATYVPIYRGQKDVSCPYCTSRFVPTQEGQLCTVCELSVVGADASGLLCSPAQIR*