AlfalfaGEDB | MsG0180006145.01

M. sativa cultivar ZhongmuNo.1 / MsG0180006145.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006145.01.T01	XP_024626420.1	48.387	62	32	0	1	62	62	123	3.28E-11	64.7

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006145.01.T01	A0A396GXA3	52.459	61	29	0	1	61	289	349	1.64e-11	67.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180006145.01	MsG0380016285.01	0.805432	1.338931e-49	7.082533e-47
MsG0180006145.01	MsG0380017202.01	0.810852	9.379579e-51	5.717000e-48
MsG0180006145.01	MsG0580025832.01	0.811198	7.891385e-51	4.854025e-48
MsG0180006145.01	MsG0580026122.01	0.812392	4.339147e-51	2.755617e-48
MsG0180006145.01	MsG0580028428.01	0.818623	1.780085e-52	1.339186e-49
MsG0180006145.01	MsG0580029925.01	0.821196	4.591385e-53	3.710547e-50
MsG0180006145.01	MsG0780039139.01	0.801144	1.034272e-48	4.902945e-46
MsG0180006145.01	MsG0780039178.01	0.821467	3.976471e-53	3.237584e-50
MsG0180006145.01	MsG0880043157.01	0.805464	1.317975e-49	6.977724e-47
MsG0180006145.01	MsG0880044209.01	0.804380	2.221499e-49	1.143711e-46
MsG0180006145.01	MsG0880045992.01	0.807834	4.164857e-50	2.343757e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006145.01.T01	MTR_7g032380	52.459	61	29	0	1	61	295	355	4.41e-15	67.8
MsG0180006145.01.T01	MTR_7g033915	48.387	62	32	0	1	62	304	365	2.21e-14	65.9
MsG0180006145.01.T01	MTR_1g041245	49.180	61	31	0	1	61	325	385	6.69e-14	64.3
MsG0180006145.01.T01	MTR_7g032370	49.123	57	29	0	5	61	295	351	3.64e-12	59.3
MsG0180006145.01.T01	MTR_7g033990	51.852	54	26	0	1	54	322	375	3.92e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTATTTATAGCGTATATTT+TGG	0.176771	1:+100695755	MsG0180006145.01.T01:CDS
CTTACAAAGGATCGACTGTT+GGG	0.467482	1:+100695730	MsG0180006145.01.T01:CDS
ACAATATTTGCATCAGAGCT+TGG	0.473610	1:-100695842	None:intergenic
ACAAATAAAATTTGACATGT+AGG	0.476036	1:-100695882	None:intergenic
TATTTGTTGAGGAAGGTTAG+TGG	0.486522	1:+100695898	MsG0180006145.01.T01:CDS
TCTTACAAAGGATCGACTGT+TGG	0.496130	1:+100695729	MsG0180006145.01.T01:CDS
CACAAGGGGATTTAAACATA+AGG	0.517250	1:+100695817	MsG0180006145.01.T01:CDS
TATGTTTAAATCCCCTTGTG+AGG	0.525248	1:-100695814	None:intergenic
GCAAATATTGTTGTGTCCAA+GGG	0.564229	1:+100695854	MsG0180006145.01.T01:CDS
TGCAAATATTGTTGTGTCCA+AGG	0.571018	1:+100695853	MsG0180006145.01.T01:CDS
TGACATGTAGGAGAGCCCCT+TGG	0.621069	1:-100695870	None:intergenic
ACCTCGAGCTTGTCTTACAA+AGG	0.635809	1:+100695717	None:intergenic
CAAATATTGTTGTGTCCAAG+GGG	0.648865	1:+100695855	MsG0180006145.01.T01:CDS
GTGACGATTAAAGCCTCACA+AGG	0.648870	1:+100695801	MsG0180006145.01.T01:CDS
TGACGATTAAAGCCTCACAA+GGG	0.662253	1:+100695802	MsG0180006145.01.T01:CDS
GACGATTAAAGCCTCACAAG+GGG	0.669603	1:+100695803	MsG0180006145.01.T01:CDS
TCCTTTGTAAGACAAGCTCG+AGG	0.719426	1:-100695718	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAATAAAATTTGACATGT+AGG	-	Chr1:100695885-100695904	None:intergenic	20.0%
!!!	ATGTCAAATTTTATTTGTTG+AGG	+	Chr1:100695887-100695906	MsG0180006145.01.T01:CDS	20.0%
!!!	TCTATTTATAGCGTATATTT+TGG	+	Chr1:100695755-100695774	MsG0180006145.01.T01:CDS	20.0%
!!!	CAAATTTTATTTGTTGAGGA+AGG	+	Chr1:100695891-100695910	MsG0180006145.01.T01:CDS	25.0%
	ACAATATTTGCATCAGAGCT+TGG	-	Chr1:100695845-100695864	None:intergenic	35.0%
	CAAATATTGTTGTGTCCAAG+GGG	+	Chr1:100695855-100695874	MsG0180006145.01.T01:CDS	35.0%
	CACAAGGGGATTTAAACATA+AGG	+	Chr1:100695817-100695836	MsG0180006145.01.T01:CDS	35.0%
	GCAAATATTGTTGTGTCCAA+GGG	+	Chr1:100695854-100695873	MsG0180006145.01.T01:CDS	35.0%
	TATGTTTAAATCCCCTTGTG+AGG	-	Chr1:100695817-100695836	None:intergenic	35.0%
	TGCAAATATTGTTGTGTCCA+AGG	+	Chr1:100695853-100695872	MsG0180006145.01.T01:CDS	35.0%
	CTTACAAAGGATCGACTGTT+GGG	+	Chr1:100695730-100695749	MsG0180006145.01.T01:CDS	40.0%
	TCTTACAAAGGATCGACTGT+TGG	+	Chr1:100695729-100695748	MsG0180006145.01.T01:CDS	40.0%
	TGACGATTAAAGCCTCACAA+GGG	+	Chr1:100695802-100695821	MsG0180006145.01.T01:CDS	40.0%
	GACGATTAAAGCCTCACAAG+GGG	+	Chr1:100695803-100695822	MsG0180006145.01.T01:CDS	45.0%
	GTGACGATTAAAGCCTCACA+AGG	+	Chr1:100695801-100695820	MsG0180006145.01.T01:CDS	45.0%
	TGACATGTAGGAGAGCCCCT+TGG	-	Chr1:100695873-100695892	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	100695720	100695917	100695720	ID=MsG0180006145.01;Name=MsG0180006145.01
Chr1	mRNA	100695720	100695917	100695720	ID=MsG0180006145.01.T01;Parent=MsG0180006145.01;Name=MsG0180006145.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|65
Chr1	exon	100695720	100695917	100695720	ID=MsG0180006145.01.T01:exon:2097;Parent=MsG0180006145.01.T01
Chr1	CDS	100695720	100695917	100695720	ID=MsG0180006145.01.T01:cds;Parent=MsG0180006145.01.T01

Gene Sequence	>MsG0180006145.01.T01 TCGAGCTTGTCTTACAAAGGATCGACTGTTGGGATTCTATTTATAGCGTATATTTTGGCGAATGCGAAATTACTGAACACTGTGACGATTAAAGCCTCACAAGGGGATTTAAACATAAGGCGCCAAGCTCTGATGCAAATATTGTTGTGTCCAAGGGGCTCTCCTACATGTCAAATTTTATTTGTTGAGGAAGGTTAG
Protein sequence	>MsG0180006145.01.T01 SSLSYKGSTVGILFIAYILANAKLLNTVTIKASQGDLNIRRQALMQILLCPRGSPTCQILFVEEG*

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