AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039178.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039178.01.T01 MTR_7g067330 73.469 49 12 1 1 48 82 130 1.44e-18 77.4
MsG0780039178.01.T01 MTR_7g067120 65.517 58 19 1 1 57 82 139 1.04e-17 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 6 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATTGCTTTGCACCTTAT+TGG 0.235386 7:+59281400 MsG0780039178.01.T01:CDS
AAATGCTTAAATGCCACTTT+TGG 0.317499 7:-59281438 None:intergenic
AATATTGGTGGTAACCAATA+AGG 0.329461 7:-59281414 None:intergenic
CAAAGCAATACAATACAATC+AGG 0.483097 7:-59281389 None:intergenic
ACCACCAATATTACCAAAAG+TGG 0.486861 7:+59281425 MsG0780039178.01.T01:CDS
AGATATGAAACAACGTGTGT+TGG 0.586185 7:-59281309 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCCAAAAATTCAATCAA+AGG - Chr7:59281363-59281382 None:intergenic 20.0%
!! ACAACCTTTGATTGAATTTT+TGG + Chr7:59281356-59281375 MsG0780039178.01.T01:CDS 25.0%
CAAAGCAATACAATACAATC+AGG - Chr7:59281392-59281411 None:intergenic 30.0%
! AATATTGGTGGTAACCAATA+AGG - Chr7:59281417-59281436 None:intergenic 30.0%
! ATATCTCAAGAAAGCTTTTG+AGG + Chr7:59281326-59281345 MsG0780039178.01.T01:CDS 30.0%
! TATCTCAAGAAAGCTTTTGA+GGG + Chr7:59281327-59281346 MsG0780039178.01.T01:CDS 30.0%
!!! AAATGCTTAAATGCCACTTT+TGG - Chr7:59281441-59281460 None:intergenic 30.0%
!!! AATGCCACTTTTGGTAATAT+TGG - Chr7:59281432-59281451 None:intergenic 30.0%
ACCACCAATATTACCAAAAG+TGG + Chr7:59281425-59281444 MsG0780039178.01.T01:CDS 35.0%
AGATATGAAACAACGTGTGT+TGG - Chr7:59281312-59281331 None:intergenic 35.0%
TGTATTGCTTTGCACCTTAT+TGG + Chr7:59281400-59281419 MsG0780039178.01.T01:CDS 35.0%
!!! GCCACTTTTGGTAATATTGG+TGG - Chr7:59281429-59281448 None:intergenic 40.0%
Chromosome Type Strat End Strand Name
Chr7 gene 59281300 59281476 59281300 ID=MsG0780039178.01;Name=MsG0780039178.01
Chr7 mRNA 59281300 59281476 59281300 ID=MsG0780039178.01.T01;Parent=MsG0780039178.01;Name=MsG0780039178.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|58
Chr7 exon 59281300 59281476 59281300 ID=MsG0780039178.01.T01:exon:6986;Parent=MsG0780039178.01.T01
Chr7 CDS 59281300 59281476 59281300 ID=MsG0780039178.01.T01:cds;Parent=MsG0780039178.01.T01
Gene Sequence

>MsG0780039178.01.T01

ATGGACATTCCAACACACGTTGTTTCATATCTCAAGAAAGCTTTTGAGGGTCTTCAACAACCTTTGATTGAATTTTTGGAAAAATTCAACCTGATTGTATTGTATTGCTTTGCACCTTATTGGTTACCACCAATATTACCAAAAGTGGCATTTAAGCATTTATTTCTCAATTTTTAG

Protein sequence

>MsG0780039178.01.T01

MDIPTHVVSYLKKAFEGLQQPLIEFLEKFNLIVLYCFAPYWLPPILPKVAFKHLFLNF*