Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006707.01.T01 | XP_003593550.1 | 97.71 | 524 | 10 | 2 | 1 | 523 | 1 | 523 | 0 | 1022 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006707.01 | MsG0380013475.01 | 0.803238 | 3.835384e-49 | 1.917454e-46 |
| MsG0280006707.01 | MsG0480018513.01 | 0.821651 | 3.605865e-53 | 2.951131e-50 |
| MsG0280006707.01 | MsG0780040669.01 | 0.823520 | 1.325031e-53 | 1.143570e-50 |
| MsG0280006707.01 | MsG0880042918.01 | 0.803313 | 3.700965e-49 | 1.853771e-46 |
| MsG0280006707.01 | MsG0880042939.01 | 0.812688 | 3.738596e-51 | 2.392810e-48 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006707.01.T01 | MTR_2g013310 | 97.710 | 524 | 10 | 2 | 1 | 523 | 1 | 523 | 0.0 | 1022 |
| MsG0280006707.01.T01 | MTR_2g013310 | 98.222 | 450 | 8 | 0 | 74 | 523 | 1 | 450 | 0.0 | 884 |
| MsG0280006707.01.T01 | MTR_7g446340 | 66.858 | 522 | 166 | 2 | 7 | 522 | 4 | 524 | 0.0 | 707 |
| MsG0280006707.01.T01 | MTR_6g007340 | 66.858 | 522 | 166 | 2 | 7 | 522 | 4 | 524 | 0.0 | 707 |
| MsG0280006707.01.T01 | MTR_4g069500 | 52.632 | 494 | 213 | 5 | 45 | 517 | 22 | 515 | 2.61e-179 | 514 |
| MsG0280006707.01.T01 | MTR_3g116240 | 51.423 | 492 | 219 | 4 | 29 | 504 | 8 | 495 | 7.02e-176 | 505 |
| MsG0280006707.01.T01 | MTR_4g072030 | 52.017 | 471 | 213 | 4 | 45 | 502 | 19 | 489 | 3.17e-166 | 481 |
| MsG0280006707.01.T01 | MTR_4g069500 | 52.258 | 465 | 201 | 5 | 74 | 517 | 1 | 465 | 1.07e-164 | 475 |
| MsG0280006707.01.T01 | MTR_6g088450 | 48.542 | 480 | 239 | 3 | 21 | 492 | 23 | 502 | 2.30e-163 | 474 |
| MsG0280006707.01.T01 | MTR_8g103500 | 48.739 | 476 | 229 | 5 | 34 | 500 | 2 | 471 | 3.82e-154 | 449 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006707.01.T01 | AT2G20780 | 66.219 | 521 | 171 | 3 | 5 | 522 | 4 | 522 | 0.0 | 692 |
| MsG0280006707.01.T01 | AT2G20780 | 68.707 | 441 | 136 | 1 | 82 | 522 | 26 | 464 | 0.0 | 613 |
| MsG0280006707.01.T01 | AT3G18830 | 49.495 | 495 | 233 | 5 | 39 | 517 | 22 | 515 | 1.24e-164 | 478 |
| MsG0280006707.01.T01 | AT2G16120 | 47.764 | 492 | 234 | 4 | 46 | 514 | 19 | 510 | 5.15e-158 | 460 |
| MsG0280006707.01.T01 | AT2G16130 | 46.800 | 500 | 247 | 4 | 31 | 511 | 1 | 500 | 6.54e-158 | 459 |
| MsG0280006707.01.T01 | AT4G36670 | 49.790 | 476 | 224 | 3 | 50 | 510 | 14 | 489 | 1.20e-156 | 456 |
| MsG0280006707.01.T01 | AT2G18480 | 51.398 | 465 | 212 | 3 | 50 | 500 | 19 | 483 | 1.88e-153 | 448 |
| MsG0280006707.01.T01 | AT2G43330 | 34.273 | 461 | 286 | 5 | 50 | 501 | 28 | 480 | 2.69e-73 | 241 |
| MsG0280006707.01.T01 | AT5G16150 | 35.639 | 477 | 291 | 8 | 25 | 497 | 75 | 539 | 4.90e-72 | 239 |
| MsG0280006707.01.T01 | AT5G16150 | 35.639 | 477 | 291 | 8 | 25 | 497 | 75 | 539 | 4.90e-72 | 239 |
Find 115 sgRNAs with CRISPR-Local
Find 189 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTTCTCCTGCCATTGCT+AGG | 0.457205 | 2:-5207574 | MsG0280006707.01.T01:CDS |
| AAGAAGAAGGGTTGGCAACC+AGG | 0.445784 | 2:-5208878 | MsG0280006707.01.T01:CDS |
| AAGCCTCAAAGGAAATATTT+CGG | 0.407884 | 2:+5206812 | None:intergenic |
| AATTCTGTTCTTCTCGGCTA+TGG | 0.438550 | 2:-5208804 | MsG0280006707.01.T01:intron |
| AATTTCTCCTGCCATTGCTA+GGG | 0.500079 | 2:-5207573 | MsG0280006707.01.T01:CDS |
| ACAAGAAAGTGAGGTAGAAA+TGG | 0.359850 | 2:-5206565 | MsG0280006707.01.T01:CDS |
| ACAAGCCATCTTGGAGATTC+AGG | 0.338176 | 2:+5207386 | None:intergenic |
| ACATGCAAAGATATATTTCT+TGG | 0.325320 | 2:+5208845 | None:intergenic |
| ACCAAAGCAATAACAGTAGC+CGG | 0.542438 | 2:-5206711 | MsG0280006707.01.T01:CDS |
| ACCTATCATCAATACTTTAA+AGG | 0.239845 | 2:+5207658 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGAAACTAATAGGTTC+TGG | + | Chr2:5207504-5207523 | None:intergenic | 30.0% | |
| AAGCCTCAAAGGAAATATTT+CGG | + | Chr2:5208714-5208733 | None:intergenic | 30.0% | |
| AATTACTACATAAACATCGC+TGG | - | Chr2:5207556-5207575 | MsG0280006707.01.T01:CDS | 30.0% | |
| ACCAAAAACAAAGAAAGTTC+CGG | + | Chr2:5208834-5208853 | None:intergenic | 30.0% | |
| ACTTAAATCTGGTAATCGAT+AGG | - | Chr2:5207478-5207497 | MsG0280006707.01.T01:CDS | 30.0% | |
| AGCAATTATATTCATTCAGG+AGG | - | Chr2:5207668-5207687 | MsG0280006707.01.T01:CDS | 30.0% | |
| AGTTATAAGCATTCTTCGAA+CGG | + | Chr2:5208330-5208349 | None:intergenic | 30.0% | |
| ATGTACAAGAAAATGGGAAT+GGG | - | Chr2:5206546-5206565 | MsG0280006707.01.T01:CDS | 30.0% | |
| ATTCTCCAATTTATATGCGA+TGG | + | Chr2:5208059-5208078 | None:intergenic | 30.0% | |
| CAAAATTTATGAAGATCTCG+GGG | + | Chr2:5207992-5208011 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 5206533 | 5209012 | 5206533 | ID=MsG0280006707.01;Name=MsG0280006707.01 |
| Chr2 | mRNA | 5206533 | 5209012 | 5206533 | ID=MsG0280006707.01.T01;Parent=MsG0280006707.01;Name=MsG0280006707.01.T01;_AED=0.26;_eAED=0.27;_QI=0|0|0|1|1|1|2|0|523 |
| Chr2 | exon | 5208805 | 5209012 | 5208805 | ID=MsG0280006707.01.T01:exon:2094;Parent=MsG0280006707.01.T01 |
| Chr2 | exon | 5206533 | 5207896 | 5206533 | ID=MsG0280006707.01.T01:exon:2093;Parent=MsG0280006707.01.T01 |
| Chr2 | CDS | 5208805 | 5209012 | 5208805 | ID=MsG0280006707.01.T01:cds;Parent=MsG0280006707.01.T01 |
| Chr2 | CDS | 5206533 | 5207896 | 5206533 | ID=MsG0280006707.01.T01:cds;Parent=MsG0280006707.01.T01 |
| Gene Sequence |
>MsG0280006707.01.T01 ATGGGGTTGGTTGATGTACAAGAAAATGGGAATGGGAATGGAGATGTGATATCTGAGATTCCATTAGGGACTAAAAATAAGTACATTAGGATGACATCTGAAGAGGAAGAAGAAGAAGAAGGGTTGGCAACCAGGAAGCATGATTCCAAGAAATATATCTTTGCATGTGCTGTTTTTGCTTCTCTCAATTCTGTTCTTCTCGGCTATGACGTTGGTGTTATGAGTGGAGCAATTATATTCATTCAGGAGGATTTGAAGATAACAGAGGTCCAACAAGAAGTACTTGTTGGAATTTTGAGCATAATATCACTTTTGGGTAGTTTAGCTGGTGGGAAAACATCTGATTGGATCGGCAGAAAATGGACCATTGGTTTGGCAGCACTTATCTTCCAAACAGGTGGAGCTATTATGGCTCTTGCTCCATCCTTTAAAGTATTGATGATAGGTAGACTCATAGCAGGTGTAGGTATAGGTTTTGGGGTCATGATTGCACCTGTGTATATCGCCGAAATTTCTCCTGCCATTGCTAGGGGATCTCTCACCTCATTCCCCGAGATCTTCATAAATTTTGGAATTCTTCTTGGCTACATATCAAACTATGCGTTTTCTAAGCTTCCATCGCATATAAATTGGAGAATAATGCTTGGTGTTGGACTTATTCCTTCGGTTGTTATTGCCGTCGCGCTTTTTATCATACCTGAATCTCCAAGATGGCTTGTCGTGCAGAATAGGATTGAAGAAGCTAAACTAGTGCTGTCAAAGATAAGCGAAAGTGAGAAAGATGCCGAAGAAAAATTGCAAGAAATTCAGAAAGCTGCCGGATCAGGAAATGCGGATAAGTACGAAACAAAAGCTGTCTGGAAAGAAATTTTGTATCCTTCTCCTCCCGTTCGAAGAATGCTTATAACTGGTTGTGGAATTCAATGTTTCCAACAGATTACAGGCATAGATACAACAGTATATTATAGCCCGACGATTTTCAAGAACGCTGGAATCACAGGAAACTCTAAGCTTCTTGCAGCAACTGTTGCTGTTGGATTCACCAAAACAGTGTTCATCTTGATAGCTATACTTCTAATCGACAAATTGGGGAGAAAGCCTTTGCTTTACGTGAGCACAATTGGAATGACAATAAGTTTATTTAGCCTGAGCTTCGCACTGGCCTTTCTGAGTCACGCAAAAGTTGGGATCGCGTTGGCGATTCTTGCTGTTTGTGGAAATGTAGCTTCTTTTTCAATAGGACTTGGTCCTATATGTTGGGTGCTGTCATCCGAAATATTTCCTTTGAGGCTTAGAGCGCAAGCATCGGCTCTTGGAGCAGTGGGAAGTAGGGTAAGCAGCGGTGCAATTTCCATGTCCTTTCTATCCGTCACCAAAGCAATAACAGTAGCCGGAACTTTCTTTGTTTTTGGTGTTATTTCGTGCAGCGCTGTTGCGTTTGTTCATTACTGTGTTCCTGAGACAAAAGGAAAGAGTTTGGAAGAAATTGAAGCGCTGTTTCAAAATGTAGGTGAATCACAAGAAAGTGAGGTAGAAATGGGAGATGTGGAGCATCTGATGCAGAAGTCATGA |
| Protein sequence |
>MsG0280006707.01.T01 MGLVDVQENGNGNGDVISEIPLGTKNKYIRMTSEEEEEEEGLATRKHDSKKYIFACAVFASLNSVLLGYDVGVMSGAIIFIQEDLKITEVQQEVLVGILSIISLLGSLAGGKTSDWIGRKWTIGLAALIFQTGGAIMALAPSFKVLMIGRLIAGVGIGFGVMIAPVYIAEISPAIARGSLTSFPEIFINFGILLGYISNYAFSKLPSHINWRIMLGVGLIPSVVIAVALFIIPESPRWLVVQNRIEEAKLVLSKISESEKDAEEKLQEIQKAAGSGNADKYETKAVWKEILYPSPPVRRMLITGCGIQCFQQITGIDTTVYYSPTIFKNAGITGNSKLLAATVAVGFTKTVFILIAILLIDKLGRKPLLYVSTIGMTISLFSLSFALAFLSHAKVGIALAILAVCGNVASFSIGLGPICWVLSSEIFPLRLRAQASALGAVGSRVSSGAISMSFLSVTKAITVAGTFFVFGVISCSAVAFVHYCVPETKGKSLEEIEALFQNVGESQESEVEMGDVEHLMQKS* |
