AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042939.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042939.01.T01 MTR_8g024790 94.495 109 6 0 1 109 356 464 1.68e-68 212
MsG0880042939.01.T01 MTR_8g024770 79.279 111 19 3 1 107 344 454 4.83e-50 164
MsG0880042939.01.T01 MTR_5g030430 49.367 79 39 1 30 108 597 674 8.13e-20 83.6
MsG0880042939.01.T01 MTR_8g067280 44.304 79 43 1 30 108 566 643 7.73e-17 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042939.01.T01 AT1G32640 50.000 80 39 1 29 108 545 623 8.61e-20 83.2
MsG0880042939.01.T01 AT4G17880 36.522 115 62 4 2 109 477 587 3.24e-18 79.0
MsG0880042939.01.T01 AT5G46760 44.304 79 43 1 31 109 513 590 7.21e-17 75.1
MsG0880042939.01.T01 AT5G46830 47.826 69 35 1 31 98 434 502 1.08e-12 63.2
MsG0880042939.01.T01 AT4G00870 48.193 83 39 2 19 98 332 413 4.84e-11 58.5

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAGGATTTGGAGTTTC+AGG 0.165432 8:-16311737 MsG0880042939.01.T01:CDS
GAGATTGATGTGAAGATAAT+AGG 0.216324 8:-16311826 MsG0880042939.01.T01:CDS
AATGAGTGTATTGAAGGATT+TGG 0.327266 8:-16311746 MsG0880042939.01.T01:CDS
TCAGCCATTCTCATGAAATT+AGG 0.386390 8:-16311616 MsG0880042939.01.T01:CDS
ATCGACTGTTAATGAAGTTA+TGG 0.398979 8:-16311698 MsG0880042939.01.T01:CDS
AAGATTAATGAGTGTATTGA+AGG 0.416800 8:-16311752 MsG0880042939.01.T01:CDS
AAAAGTGAAGATGGAAATGA+TGG 0.445716 8:-16311926 None:intergenic
TCTTCCGCGCTCATCTCGTT+AGG 0.463829 8:+16311652 None:intergenic
CTGACCTAATTTCATGAGAA+TGG 0.482002 8:+16311612 None:intergenic
ACACTCATTAATCTTGCACT+AGG 0.528247 8:+16311760 None:intergenic
TCAAAAGCATTAACATTACA+AGG 0.560113 8:+16311847 None:intergenic
GATAATAGGAAATGATGCTA+TGG 0.577854 8:-16311812 MsG0880042939.01.T01:CDS
TGCAACGGCAACTTCAACTG+TGG 0.578390 8:-16311884 MsG0880042939.01.T01:CDS
TGTCGATAATAACAGTGCAA+CGG 0.631393 8:-16311899 MsG0880042939.01.T01:CDS
CACTCATTAATCTTGCACTA+GGG 0.639299 8:+16311761 None:intergenic
AGTTCCTAACGAGATGAGCG+CGG 0.706023 8:-16311656 MsG0880042939.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGATTAATGAGTGTATTGA+AGG - Chr8:16311773-16311792 MsG0880042939.01.T01:CDS 25.0%
!!! TAACATTACAAGGTCTTTTT+TGG + Chr8:16311671-16311690 None:intergenic 25.0%
!!! TCAAAAGCATTAACATTACA+AGG + Chr8:16311681-16311700 None:intergenic 25.0%
AATGAGTGTATTGAAGGATT+TGG - Chr8:16311779-16311798 MsG0880042939.01.T01:CDS 30.0%
ATCGACTGTTAATGAAGTTA+TGG - Chr8:16311827-16311846 MsG0880042939.01.T01:CDS 30.0%
GAGATTGATGTGAAGATAAT+AGG - Chr8:16311699-16311718 MsG0880042939.01.T01:CDS 30.0%
GATAATAGGAAATGATGCTA+TGG - Chr8:16311713-16311732 MsG0880042939.01.T01:CDS 30.0%
ACACTCATTAATCTTGCACT+AGG + Chr8:16311768-16311787 None:intergenic 35.0%
CACTCATTAATCTTGCACTA+GGG + Chr8:16311767-16311786 None:intergenic 35.0%
CTGACCTAATTTCATGAGAA+TGG + Chr8:16311916-16311935 None:intergenic 35.0%
TCAGCCATTCTCATGAAATT+AGG - Chr8:16311909-16311928 MsG0880042939.01.T01:CDS 35.0%
TGTCGATAATAACAGTGCAA+CGG - Chr8:16311626-16311645 MsG0880042939.01.T01:CDS 35.0%
! ATTGAAGGATTTGGAGTTTC+AGG - Chr8:16311788-16311807 MsG0880042939.01.T01:CDS 35.0%
AGTTCCTAACGAGATGAGCG+CGG - Chr8:16311869-16311888 MsG0880042939.01.T01:CDS 50.0%
TGCAACGGCAACTTCAACTG+TGG - Chr8:16311641-16311660 MsG0880042939.01.T01:CDS 50.0%
TCTTCCGCGCTCATCTCGTT+AGG + Chr8:16311876-16311895 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 16311609 16311938 16311609 ID=MsG0880042939.01;Name=MsG0880042939.01
Chr8 mRNA 16311609 16311938 16311609 ID=MsG0880042939.01.T01;Parent=MsG0880042939.01;Name=MsG0880042939.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|109
Chr8 exon 16311609 16311938 16311609 ID=MsG0880042939.01.T01:exon:4279;Parent=MsG0880042939.01.T01
Chr8 CDS 16311609 16311938 16311609 ID=MsG0880042939.01.T01:cds;Parent=MsG0880042939.01.T01
Gene Sequence

>MsG0880042939.01.T01

ATGGAAATGATGGAAATTGTCGATAATAACAGTGCAACGGCAACTTCAACTGTGGTAGACCAAAAAAGACCTTGTAATGTTAATGCTTTTGAGATTGATGTGAAGATAATAGGAAATGATGCTATGGTGAGAGTTCAAAGTGAGAATGTTAATCACCCTAGTGCAAGATTAATGAGTGTATTGAAGGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACTGTTAATGAAGTTATGGTTCAAGATGTTGTTGTTCGAGTTCCTAACGAGATGAGCGCGGAAGAGAGTCTAATTAGATCAGCCATTCTCATGAAATTAGGTCAGTAG

Protein sequence

>MsG0880042939.01.T01

MEMMEIVDNNSATATSTVVDQKRPCNVNAFEIDVKIIGNDAMVRVQSENVNHPSARLMSVLKDLEFQVHHASISTVNEVMVQDVVVRVPNEMSAEESLIRSAILMKLGQ*