AlfalfaGEDB | MsG0280006744.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006744.01

KEGG: -
GO: GO:0003677 GO:0005634
PFAM: PF03110

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006744.01.T01	XP_003593617.1	89.189	111	10	1	1	109	1	111	1.05E-50	168

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006744.01.T01	Q38741	52.294	109	42	3	1	109	1	99	1.07E-28	104

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006744.01.T01	G7ISD5	89.189	111	10	1	1	109	1	111	5.02e-51	168

TFs/TRs:

Gene ID	Type	Classification
MsG0280006744.01.T01	TF	SBP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048660.01	MsG0280006744.01	0.822620	2.148368e-53	1.807731e-50
MsG0280006744.01	MsG0580025612.01	0.811690	6.170315e-51	3.844800e-48
MsG0280006744.01	MsG0880041861.01	0.800324	1.520455e-48	7.059431e-46
MsG0280006744.01	MsG0880041864.01	0.805596	1.236856e-49	6.570114e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006744.01.T01	MTR_2g014200	89.189	111	10	1	1	109	1	111	1.27e-54	168
MsG0280006744.01.T01	MTR_4g088555	58.559	111	43	2	1	109	1	110	1.10e-31	110
MsG0280006744.01.T01	MTR_8g005960	64.286	56	20	0	54	109	179	234	2.82e-20	84.3
MsG0280006744.01.T01	MTR_1g035010	50.575	87	38	2	23	109	70	151	1.43e-19	83.6
MsG0280006744.01.T01	MTR_2g078770	56.897	58	25	0	52	109	48	105	2.43e-19	79.3
MsG0280006744.01.T01	MTR_7g110320	50.649	77	28	1	33	109	126	192	3.98e-19	82.4
MsG0280006744.01.T01	MTR_8g463140	62.000	50	19	0	60	109	63	112	2.42e-18	76.6
MsG0280006744.01.T01	MTR_7g110320	50.649	77	28	1	33	109	126	192	4.42e-18	79.3
MsG0280006744.01.T01	MTR_2g046550	64.583	48	17	0	62	109	139	186	1.49e-17	77.8
MsG0280006744.01.T01	MTR_1g086250	56.452	62	26	1	49	109	133	194	6.35e-17	76.3
MsG0280006744.01.T01	MTR_5g046670	51.562	64	31	0	46	109	228	291	6.76e-17	75.9
MsG0280006744.01.T01	MTR_3g085180	49.231	65	32	1	46	109	148	212	1.28e-16	75.1
MsG0280006744.01.T01	MTR_7g092930	59.322	59	20	1	55	109	57	115	1.44e-16	74.3
MsG0280006744.01.T01	MTR_1g053715	59.649	57	23	0	53	109	65	121	1.95e-16	74.3
MsG0280006744.01.T01	MTR_7g444860	58.929	56	23	0	54	109	141	196	1.97e-16	73.9
MsG0280006744.01.T01	MTR_3g099080	62.264	53	20	0	57	109	93	145	2.48e-16	73.9
MsG0280006744.01.T01	MTR_7g028740	61.111	54	21	0	56	109	71	124	2.54e-16	73.9
MsG0280006744.01.T01	MTR_4g109770	62.264	53	20	0	57	109	118	170	4.99e-16	73.6
MsG0280006744.01.T01	MTR_2g461920	64.583	48	17	0	62	109	171	218	9.63e-16	72.8
MsG0280006744.01.T01	MTR_8g096780	59.259	54	22	0	56	109	93	146	1.37e-14	69.3
MsG0280006744.01.T01	MTR_8g080690	53.846	52	24	0	60	111	149	200	2.02e-13	65.9
MsG0280006744.01.T01	MTR_8g080680	54.000	50	23	0	60	109	130	179	8.80e-13	63.9
MsG0280006744.01.T01	MTR_8g080670	54.000	50	23	0	60	109	160	209	3.09e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006744.01.T01	AT1G02065	62.295	61	23	0	59	119	185	245	2.52e-21	86.3
MsG0280006744.01.T01	AT1G76580	68.000	50	16	0	60	109	112	161	1.06e-19	84.3
MsG0280006744.01.T01	AT2G33810	63.333	60	19	1	53	109	42	101	1.29e-19	79.0
MsG0280006744.01.T01	AT1G20980	62.295	61	21	1	49	109	109	167	1.30e-19	84.0
MsG0280006744.01.T01	AT1G02065	68.627	51	16	0	59	109	185	235	1.36e-19	82.8
MsG0280006744.01.T01	AT1G76580	68.000	50	16	0	60	109	112	161	1.72e-19	83.6
MsG0280006744.01.T01	AT1G53160	55.072	69	31	0	41	109	33	101	1.89e-19	79.7
MsG0280006744.01.T01	AT1G53160	55.072	69	31	0	41	109	33	101	1.89e-19	79.7
MsG0280006744.01.T01	AT1G53160	55.072	69	31	0	41	109	33	101	1.97e-19	79.3
MsG0280006744.01.T01	AT1G76580	68.000	50	16	0	60	109	112	161	2.03e-19	83.2
MsG0280006744.01.T01	AT3G60030	60.938	64	20	2	50	109	112	174	5.05e-19	82.0
MsG0280006744.01.T01	AT2G47070	55.224	67	26	1	43	109	91	153	2.03e-18	80.5
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	3.39e-17	76.3
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	5.14e-17	76.3
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	5.14e-17	76.3
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	5.14e-17	76.3
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	5.14e-17	76.3
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	5.14e-17	76.3
MsG0280006744.01.T01	AT1G27370	47.887	71	35	1	41	109	153	223	5.14e-17	76.3
MsG0280006744.01.T01	AT5G43270	58.824	51	21	0	59	109	166	216	1.85e-16	74.7
MsG0280006744.01.T01	AT5G43270	58.824	51	21	0	59	109	166	216	1.85e-16	74.7
MsG0280006744.01.T01	AT5G43270	58.824	51	21	0	59	109	166	216	1.85e-16	74.7
MsG0280006744.01.T01	AT1G27360	49.296	71	35	1	40	109	152	222	4.83e-16	73.6
MsG0280006744.01.T01	AT1G27360	49.296	71	35	1	40	109	152	222	4.83e-16	73.6
MsG0280006744.01.T01	AT1G27360	49.296	71	35	1	40	109	152	222	4.83e-16	73.6
MsG0280006744.01.T01	AT1G27360	49.296	71	35	1	40	109	152	222	4.83e-16	73.6
MsG0280006744.01.T01	AT1G69170	56.452	62	25	1	50	109	110	171	6.69e-16	73.2
MsG0280006744.01.T01	AT3G15270	56.250	48	21	0	62	109	63	110	1.60e-15	69.7
MsG0280006744.01.T01	AT5G50670	52.542	59	28	0	51	109	90	148	4.85e-15	70.5
MsG0280006744.01.T01	AT5G50670	52.542	59	28	0	51	109	90	148	4.85e-15	70.5
MsG0280006744.01.T01	AT5G50570	52.542	59	28	0	51	109	90	148	4.85e-15	70.5
MsG0280006744.01.T01	AT5G50570	52.542	59	28	0	51	109	90	148	4.85e-15	70.5
MsG0280006744.01.T01	AT3G57920	47.436	78	36	1	32	109	34	106	9.05e-15	69.7
MsG0280006744.01.T01	AT2G42200	54.000	50	23	0	60	109	72	121	9.38e-14	67.0

Find 29 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAAACCTTATGGCGCTTA+TGG	0.248219	2:-5763086	None:intergenic
AATGGTATTCACAAACCTTA+TGG	0.308780	2:-5763095	None:intergenic
TTTACTTATGGATGGAAGTT+GGG	0.337434	2:+5762849	None:intergenic
AAATATTGAGGTTCAATATA+AGG	0.346436	2:-5763241	None:intergenic
GGTTTGTGAATACCATTCTA+AGG	0.368542	2:+5763101	MsG0280006744.01.T01:CDS
TTGTCAACCTGCTACATTGC+TGG	0.372075	2:-5763164	None:intergenic
GAAGAGGAAGATGTGAGTTA+TGG	0.375825	2:+5762931	MsG0280006744.01.T01:CDS
CTTTACTTATGGATGGAAGT+TGG	0.392836	2:+5762848	None:intergenic
GAGATGATGGGAAGAAGAAA+AGG	0.443829	2:+5762953	MsG0280006744.01.T01:CDS
TGAAGTAGAAGTAGAAGAAG+AGG	0.463169	2:+5762915	MsG0280006744.01.T01:CDS
ATGGATGGAAGTTGGGGTGA+GGG	0.465253	2:+5762856	MsG0280006744.01.T01:CDS
GATGTGAGTTATGGAGATGA+TGG	0.466595	2:+5762940	MsG0280006744.01.T01:CDS
AACAGGAAAGGGTCAAAAGC+TGG	0.479614	2:+5762997	MsG0280006744.01.T01:CDS
TACTAATACTAAGATATTCA+AGG	0.487533	2:-5763209	None:intergenic
TATGGATGGAAGTTGGGGTG+AGG	0.500828	2:+5762855	None:intergenic
TGACTGATCATCTTTACAAC+AGG	0.501584	2:+5762980	MsG0280006744.01.T01:CDS
ATCATCTTTACAACAGGAAA+GGG	0.510630	2:+5762986	MsG0280006744.01.T01:CDS
GATTTACCTTTACTTATGGA+TGG	0.521525	2:+5762841	None:intergenic
TTGAAGCTCTGAAATGAGTA+CGG	0.530837	2:-5763130	None:intergenic
GCAGTACCATAAGCGCCATA+AGG	0.534642	2:+5763080	MsG0280006744.01.T01:CDS
TTACTTATGGATGGAAGTTG+GGG	0.539498	2:+5762850	None:intergenic
GATCATCTTTACAACAGGAA+AGG	0.569404	2:+5762985	MsG0280006744.01.T01:CDS
GTACGGAATGAGCCTTAGAA+TGG	0.572289	2:-5763113	None:intergenic
AGATGATGGGAAGAAGAAAA+GGG	0.572790	2:+5762954	MsG0280006744.01.T01:CDS
CAGTTATCAACTTGACAAGA+AGG	0.576877	2:-5763033	None:intergenic
ATGTGAGTTATGGAGATGAT+GGG	0.595276	2:+5762941	MsG0280006744.01.T01:CDS
TCATTTCAGAGCTTCAACAA+AGG	0.599380	2:+5763136	MsG0280006744.01.T01:CDS
TGATGGGAAGAAGAAAAGGG+TGG	0.655448	2:+5762957	MsG0280006744.01.T01:CDS
AGGAAAGGGTCAAAAGCTGG+AGG	0.666819	2:+5763000	MsG0280006744.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGATATTCAAGGAAAAAAA+AGG	-	Chr2:5763202-5763221	None:intergenic	20.0%
!!	TACTAATACTAAGATATTCA+AGG	-	Chr2:5763212-5763231	None:intergenic	20.0%
!	AGGAAAAAAAAGGTGAAATT+AGG	-	Chr2:5763192-5763211	None:intergenic	25.0%
!	GGAAAAAAAAGGTGAAATTA+GGG	-	Chr2:5763191-5763210	None:intergenic	25.0%
	AATGGTATTCACAAACCTTA+TGG	-	Chr2:5763098-5763117	None:intergenic	30.0%
	ATCATCTTTACAACAGGAAA+GGG	+	Chr2:5762986-5763005	MsG0280006744.01.T01:CDS	30.0%
	ATGTGAGTTATGGAGATGAT+GGG	+	Chr2:5762941-5762960	MsG0280006744.01.T01:CDS	35.0%
	CAGTTATCAACTTGACAAGA+AGG	-	Chr2:5763036-5763055	None:intergenic	35.0%
	GATCATCTTTACAACAGGAA+AGG	+	Chr2:5762985-5763004	MsG0280006744.01.T01:CDS	35.0%
	GGTTTGTGAATACCATTCTA+AGG	+	Chr2:5763101-5763120	MsG0280006744.01.T01:CDS	35.0%
	TCATTTCAGAGCTTCAACAA+AGG	+	Chr2:5763136-5763155	MsG0280006744.01.T01:CDS	35.0%
	TGAAGTAGAAGTAGAAGAAG+AGG	+	Chr2:5762915-5762934	MsG0280006744.01.T01:CDS	35.0%
	TGACTGATCATCTTTACAAC+AGG	+	Chr2:5762980-5762999	MsG0280006744.01.T01:CDS	35.0%
!!	AGATGATGGGAAGAAGAAAA+GGG	+	Chr2:5762954-5762973	MsG0280006744.01.T01:CDS	35.0%
!!	TTGAAGCTCTGAAATGAGTA+CGG	-	Chr2:5763133-5763152	None:intergenic	35.0%
	GAAGAGGAAGATGTGAGTTA+TGG	+	Chr2:5762931-5762950	MsG0280006744.01.T01:CDS	40.0%
	GATGTGAGTTATGGAGATGA+TGG	+	Chr2:5762940-5762959	MsG0280006744.01.T01:CDS	40.0%
!!	GAGATGATGGGAAGAAGAAA+AGG	+	Chr2:5762953-5762972	MsG0280006744.01.T01:CDS	40.0%
	AACAGGAAAGGGTCAAAAGC+TGG	+	Chr2:5762997-5763016	MsG0280006744.01.T01:CDS	45.0%
	CACAAACCTTATGGCGCTTA+TGG	-	Chr2:5763089-5763108	None:intergenic	45.0%
	GTACGGAATGAGCCTTAGAA+TGG	-	Chr2:5763116-5763135	None:intergenic	45.0%
	TGATGGGAAGAAGAAAAGGG+TGG	+	Chr2:5762957-5762976	MsG0280006744.01.T01:CDS	45.0%
	TTGTCAACCTGCTACATTGC+TGG	-	Chr2:5763167-5763186	None:intergenic	45.0%
	AGGAAAGGGTCAAAAGCTGG+AGG	+	Chr2:5763000-5763019	MsG0280006744.01.T01:CDS	50.0%
	ATGGATGGAAGTTGGGGTGA+GGG	+	Chr2:5762856-5762875	MsG0280006744.01.T01:CDS	50.0%
	GCAGTACCATAAGCGCCATA+AGG	+	Chr2:5763080-5763099	MsG0280006744.01.T01:CDS	50.0%
!	GGTTTTGCCAGCAATGTAGC+AGG	+	Chr2:5763157-5763176	MsG0280006744.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	5762856	5763251	5762856	ID=MsG0280006744.01;Name=MsG0280006744.01
Chr2	mRNA	5762856	5763251	5762856	ID=MsG0280006744.01.T01;Parent=MsG0280006744.01;Name=MsG0280006744.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|131
Chr2	exon	5762856	5763251	5762856	ID=MsG0280006744.01.T01:exon:679;Parent=MsG0280006744.01.T01
Chr2	CDS	5762856	5763251	5762856	ID=MsG0280006744.01.T01:cds;Parent=MsG0280006744.01.T01

Gene Sequence

>MsG0280006744.01.T01

ATGGATGGAAGTTGGGGTGAGGGAAAGAGAATCTATGAGTACAGAGAAGAAGATGAGTATGAAGTAGAAGTAGAAGAAGAGGAAGATGTGAGTTATGGAGATGATGGGAAGAAGAAAAGGGTGGTGACTGATCATCTTTACAACAGGAAAGGGTCAAAAGCTGGAGGATCAGTGACACCTTCTTGTCAAGTTGATAACTGTAATGCTGATCTAAGTGTTGCTAAGCAGTACCATAAGCGCCATAAGGTTTGTGAATACCATTCTAAGGCTCATTCCGTACTCATTTCAGAGCTTCAACAAAGGTTTTGCCAGCAATGTAGCAGGTTGACAAACCCTAATTTCACCTTTTTTTTCCTTGAATATCTTAGTATTAGTATTCGTATATCCTTATATTGA

Protein sequence

>MsG0280006744.01.T01

MDGSWGEGKRIYEYREEDEYEVEVEEEEDVSYGDDGKKKRVVTDHLYNRKGSKAGGSVTPSCQVDNCNADLSVAKQYHKRHKVCEYHSKAHSVLISELQQRFCQQCSRLTNPNFTFFFLEYLSISIRISLY*