AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006834.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006834.01.T01 MTR_2g016030 96.471 85 2 1 1 84 1 85 4.72e-55 167
MsG0280006834.01.T01 MTR_4g082060 62.687 67 25 0 18 84 94 160 3.57e-27 102
MsG0280006834.01.T01 MTR_7g010950 62.319 69 25 1 17 84 105 173 9.22e-27 101
MsG0280006834.01.T01 MTR_8g044220 68.852 61 18 1 25 84 96 156 1.02e-26 101
MsG0280006834.01.T01 MTR_6g012450 66.667 66 21 1 19 84 110 174 1.25e-26 101
MsG0280006834.01.T01 MTR_6g027460 73.214 56 14 1 30 84 79 134 6.40e-25 95.5
MsG0280006834.01.T01 MTR_5g041380 60.870 69 25 1 18 84 97 165 7.47e-25 95.5
MsG0280006834.01.T01 MTR_7g086780 70.690 58 16 1 28 84 80 137 4.85e-24 93.6
MsG0280006834.01.T01 MTR_5g041400 69.811 53 16 0 32 84 105 157 9.03e-24 92.4
MsG0280006834.01.T01 MTR_6g027450 69.643 56 16 1 30 84 72 127 1.65e-23 91.3
MsG0280006834.01.T01 MTR_5g041420 75.556 45 11 0 40 84 132 176 2.20e-23 90.9
MsG0280006834.01.T01 MTR_5g041530 61.667 60 22 1 25 84 120 178 3.93e-23 90.9
MsG0280006834.01.T01 MTR_3g435480 53.333 75 26 1 19 84 100 174 7.81e-23 90.5
MsG0280006834.01.T01 MTR_4g022370 69.048 42 13 0 43 84 79 120 3.39e-20 82.8
MsG0280006834.01.T01 MTR_2g096740 51.667 60 29 0 25 84 41 100 5.55e-20 81.3
MsG0280006834.01.T01 MTR_2g096740 51.667 60 29 0 25 84 56 115 7.26e-20 81.3
MsG0280006834.01.T01 MTR_4g109980 68.293 41 13 0 44 84 38 78 1.97e-19 80.1
MsG0280006834.01.T01 MTR_7g059400 69.048 42 13 0 43 84 73 114 5.25e-19 79.3
MsG0280006834.01.T01 MTR_8g079060 74.359 39 10 0 46 84 29 67 5.93e-19 77.8
MsG0280006834.01.T01 MTR_8g068210 55.172 58 25 1 28 84 13 70 5.98e-19 79.0
MsG0280006834.01.T01 MTR_2g014170 69.231 39 12 0 46 84 45 83 9.37e-19 78.2
MsG0280006834.01.T01 MTR_8g015840 56.140 57 19 1 34 84 2 58 9.91e-19 77.0
MsG0280006834.01.T01 MTR_7g024670 75.676 37 9 0 48 84 79 115 1.08e-18 78.6
MsG0280006834.01.T01 MTR_8g479350 75.676 37 9 0 48 84 70 106 1.30e-18 78.2
MsG0280006834.01.T01 MTR_4g088580 72.973 37 10 0 48 84 57 93 1.56e-18 78.2
MsG0280006834.01.T01 MTR_2g059540 71.795 39 11 0 46 84 31 69 2.32e-18 75.5
MsG0280006834.01.T01 MTR_2g093220 55.556 54 24 0 31 84 25 78 2.33e-18 77.0
MsG0280006834.01.T01 MTR_5g031440 66.667 42 14 0 43 84 40 81 2.35e-18 77.4
MsG0280006834.01.T01 MTR_4g063780 69.048 42 13 0 43 84 72 113 2.70e-18 77.4
MsG0280006834.01.T01 MTR_2g014060 47.619 63 33 0 22 84 1 63 3.19e-18 77.0
MsG0280006834.01.T01 MTR_3g077750 66.667 39 13 0 46 84 61 99 6.11e-18 76.3
MsG0280006834.01.T01 MTR_3g091820 61.905 42 16 0 43 84 40 81 2.86e-17 74.3
MsG0280006834.01.T01 MTR_8g027295 69.231 39 12 0 46 84 24 62 3.00e-17 73.9
MsG0280006834.01.T01 MTR_4g461080 49.180 61 31 0 24 84 7 67 3.56e-17 73.6
MsG0280006834.01.T01 MTR_2g013370 66.667 39 13 0 46 84 46 84 4.37e-17 73.6
MsG0280006834.01.T01 MTR_1g056810 64.444 45 15 1 41 84 6 50 5.20e-17 73.6
MsG0280006834.01.T01 MTR_4g089095 70.270 37 11 0 48 84 52 88 6.38e-17 73.2
MsG0280006834.01.T01 MTR_1g077600 64.286 42 15 0 43 84 40 81 6.95e-17 72.8
MsG0280006834.01.T01 MTR_1g077600 64.286 42 15 0 43 84 40 81 7.59e-17 72.8
MsG0280006834.01.T01 MTR_3g090430 65.116 43 15 0 42 84 47 89 1.88e-16 72.4
MsG0280006834.01.T01 MTR_4g089095 70.270 37 11 0 48 84 18 54 4.60e-16 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006834.01.T01 AT1G29160 55.670 97 23 1 8 84 4 100 1.54e-32 111
MsG0280006834.01.T01 AT2G34140 56.989 93 24 1 8 84 4 96 1.67e-31 108
MsG0280006834.01.T01 AT5G62430 56.471 85 25 1 12 84 8 92 1.19e-27 101
MsG0280006834.01.T01 AT3G47500 63.077 65 24 0 20 84 84 148 4.82e-27 102
MsG0280006834.01.T01 AT1G26790 66.129 62 21 0 23 84 88 149 1.95e-26 99.8
MsG0280006834.01.T01 AT5G39660 84.783 46 7 0 39 84 131 176 1.54e-25 98.2
MsG0280006834.01.T01 AT5G39660 84.783 46 7 0 39 84 131 176 1.54e-25 98.2
MsG0280006834.01.T01 AT5G39660 84.783 46 7 0 39 84 131 176 1.54e-25 98.2
MsG0280006834.01.T01 AT1G69570 72.549 51 14 0 34 84 120 170 3.29e-24 94.0
MsG0280006834.01.T01 AT2G28810 71.429 42 12 0 43 84 74 115 2.91e-20 82.4
MsG0280006834.01.T01 AT2G28810 71.429 42 12 0 43 84 91 132 2.98e-20 82.8
MsG0280006834.01.T01 AT2G37590 63.265 49 18 0 36 84 79 127 5.26e-20 82.0
MsG0280006834.01.T01 AT1G07640 56.604 53 23 0 32 84 7 59 1.30e-19 80.1
MsG0280006834.01.T01 AT3G55370 69.048 42 13 0 43 84 118 159 2.05e-19 80.9
MsG0280006834.01.T01 AT1G07640 56.604 53 23 0 32 84 63 115 2.07e-19 80.5
MsG0280006834.01.T01 AT1G07640 56.604 53 23 0 32 84 71 123 2.28e-19 80.5
MsG0280006834.01.T01 AT5G60850 71.795 39 11 0 46 84 51 89 2.40e-19 80.1
MsG0280006834.01.T01 AT5G02460 69.048 42 13 0 43 84 92 133 3.21e-19 80.5
MsG0280006834.01.T01 AT3G55370 69.048 42 13 0 43 84 73 114 5.06e-19 79.3
MsG0280006834.01.T01 AT3G50410 74.359 39 10 0 46 84 30 68 5.59e-19 78.2
MsG0280006834.01.T01 AT3G55370 69.048 42 13 0 43 84 73 114 5.67e-19 79.3
MsG0280006834.01.T01 AT1G28310 69.231 39 12 0 46 84 27 65 1.00e-18 78.2
MsG0280006834.01.T01 AT5G66940 74.359 39 10 0 46 84 32 70 1.02e-18 77.0
MsG0280006834.01.T01 AT1G28310 69.231 39 12 0 46 84 51 89 1.70e-18 77.8
MsG0280006834.01.T01 AT1G51700 71.795 39 11 0 46 84 33 71 2.60e-18 75.5
MsG0280006834.01.T01 AT5G65590 63.043 46 17 0 39 84 34 79 2.66e-18 77.4
MsG0280006834.01.T01 AT3G21270 78.378 37 8 0 48 84 31 67 2.78e-18 75.5
MsG0280006834.01.T01 AT1G47655 63.636 44 16 0 41 84 24 67 2.86e-18 75.5
MsG0280006834.01.T01 AT5G62940 70.270 37 11 0 48 84 75 111 3.92e-18 77.0
MsG0280006834.01.T01 AT4G38000 49.254 67 27 1 18 84 20 79 7.75e-18 75.1
MsG0280006834.01.T01 AT2G28510 72.973 37 10 0 48 84 50 86 8.78e-18 75.5
MsG0280006834.01.T01 AT1G64620 64.286 42 15 0 43 84 46 87 9.38e-18 75.9
MsG0280006834.01.T01 AT3G52440 66.667 42 13 1 44 84 41 82 1.88e-17 74.3
MsG0280006834.01.T01 AT4G24060 61.905 42 16 0 43 84 50 91 2.29e-17 74.7
MsG0280006834.01.T01 AT3G45610 66.667 42 14 0 43 84 37 78 2.50e-17 73.6
MsG0280006834.01.T01 AT2G46590 70.270 37 11 0 48 84 70 106 2.72e-17 74.7
MsG0280006834.01.T01 AT2G46590 70.270 37 11 0 48 84 82 118 3.47e-17 74.7
MsG0280006834.01.T01 AT3G61850 55.102 49 22 0 36 84 52 100 3.92e-17 73.6
MsG0280006834.01.T01 AT3G61850 55.102 49 22 0 36 84 52 100 3.92e-17 73.6
MsG0280006834.01.T01 AT5G60200 51.613 62 27 1 26 84 32 93 3.95e-17 73.2
MsG0280006834.01.T01 AT3G61850 55.102 49 22 0 36 84 64 112 4.73e-17 73.6
MsG0280006834.01.T01 AT3G61850 55.102 49 22 0 36 84 52 100 5.19e-17 73.6
MsG0280006834.01.T01 AT3G61850 55.102 49 22 0 36 84 64 112 6.08e-17 73.6
MsG0280006834.01.T01 AT1G21340 70.270 37 11 0 48 84 40 76 5.92e-16 70.1
MsG0280006834.01.T01 AT3G52440 70.270 37 11 0 48 84 27 63 1.13e-15 69.3
MsG0280006834.01.T01 AT4G00940 70.270 37 11 0 48 84 70 106 4.67e-15 68.2
MsG0280006834.01.T01 AT4G00940 70.270 37 11 0 48 84 70 106 4.67e-15 68.2
MsG0280006834.01.T01 AT4G00940 70.270 37 11 0 48 84 70 106 4.67e-15 68.2
MsG0280006834.01.T01 AT4G21050 67.568 37 12 0 48 84 26 62 9.40e-15 66.2
MsG0280006834.01.T01 AT4G21040 67.568 37 12 0 48 84 27 63 2.23e-13 63.2
MsG0280006834.01.T01 AT4G21080 67.568 37 12 0 48 84 27 63 1.21e-12 61.2

Find 19 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACAAGGGATTAAGCTATT+TGG 0.296201 2:-6872857 MsG0280006834.01.T01:CDS
GTAAGAGCTGTCAAAGATAC+TGG 0.350596 2:-6872667 MsG0280006834.01.T01:CDS
ATGTCCAAGATGCAAGAGTA+TGG 0.357770 2:-6872744 MsG0280006834.01.T01:CDS
GTTTCCATACTCTTGCATCT+TGG 0.414049 2:+6872740 None:intergenic
TGTCAAAGATACTGGACGGC+TGG 0.464609 2:-6872659 MsG0280006834.01.T01:CDS
TCAAAGATACTGGACGGCTG+GGG 0.524887 2:-6872657 MsG0280006834.01.T01:CDS
AAAAGAAAGTGAAGATCAAA+CGG 0.525439 2:-6872795 MsG0280006834.01.T01:CDS
GTCAAAGATACTGGACGGCT+GGG 0.527396 2:-6872658 MsG0280006834.01.T01:CDS
TTGCATGAGGAATTAAAAGA+AGG 0.537463 2:-6872821 MsG0280006834.01.T01:CDS
AGAAAAGGTGAAGAGATACA+AGG 0.544735 2:-6872872 MsG0280006834.01.T01:CDS
ATACTCTTGCATCTTGGACA+TGG 0.546078 2:+6872746 None:intergenic
CAAAGATACTGGACGGCTGG+GGG 0.569997 2:-6872656 MsG0280006834.01.T01:CDS
AAAATGGCTGAAGTGAGAAA+AGG 0.579551 2:-6872887 MsG0280006834.01.T01:CDS
GAAAAGGTGAAGAGATACAA+GGG 0.603099 2:-6872871 MsG0280006834.01.T01:CDS
CACAACAATAAAATTGCATG+AGG 0.620119 2:-6872834 MsG0280006834.01.T01:CDS
GAGCTGTCAAAGATACTGGA+CGG 0.644495 2:-6872663 MsG0280006834.01.T01:CDS
TCCAACCGGAACGTTACGGA+GGG 0.647718 2:+6872633 None:intergenic
CTCCAACCGGAACGTTACGG+AGG 0.649811 2:+6872632 None:intergenic
AGAAAGTGAAGATCAAACGG+TGG 0.709703 2:-6872792 MsG0280006834.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAAGTGAAGATCAAA+CGG - Chr2:6872735-6872754 MsG0280006834.01.T01:CDS 25.0%
ATACAAGGGATTAAGCTATT+TGG - Chr2:6872673-6872692 MsG0280006834.01.T01:CDS 30.0%
CACAACAATAAAATTGCATG+AGG - Chr2:6872696-6872715 MsG0280006834.01.T01:CDS 30.0%
TTGCATGAGGAATTAAAAGA+AGG - Chr2:6872709-6872728 MsG0280006834.01.T01:CDS 30.0%
AGAAAAGGTGAAGAGATACA+AGG - Chr2:6872658-6872677 MsG0280006834.01.T01:CDS 35.0%
CAGCTCTTACAAAAATGTCT+TGG + Chr2:6872853-6872872 None:intergenic 35.0%
GAAAAGGTGAAGAGATACAA+GGG - Chr2:6872659-6872678 MsG0280006834.01.T01:CDS 35.0%
AGAAAGTGAAGATCAAACGG+TGG - Chr2:6872738-6872757 MsG0280006834.01.T01:CDS 40.0%
ATACTCTTGCATCTTGGACA+TGG + Chr2:6872787-6872806 None:intergenic 40.0%
ATGTCCAAGATGCAAGAGTA+TGG - Chr2:6872786-6872805 MsG0280006834.01.T01:CDS 40.0%
GTAAGAGCTGTCAAAGATAC+TGG - Chr2:6872863-6872882 MsG0280006834.01.T01:CDS 40.0%
GTTTCCATACTCTTGCATCT+TGG + Chr2:6872793-6872812 None:intergenic 40.0%
GAGCTGTCAAAGATACTGGA+CGG - Chr2:6872867-6872886 MsG0280006834.01.T01:CDS 45.0%
GTCAAAGATACTGGACGGCT+GGG - Chr2:6872872-6872891 MsG0280006834.01.T01:CDS 50.0%
TCAAAGATACTGGACGGCTG+GGG - Chr2:6872873-6872892 MsG0280006834.01.T01:CDS 50.0%
TGTCAAAGATACTGGACGGC+TGG - Chr2:6872871-6872890 MsG0280006834.01.T01:CDS 50.0%
CAAAGATACTGGACGGCTGG+GGG - Chr2:6872874-6872893 MsG0280006834.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 6872646 6872906 6872646 ID=MsG0280006834.01;Name=MsG0280006834.01
Chr2 mRNA 6872646 6872906 6872646 ID=MsG0280006834.01.T01;Parent=MsG0280006834.01;Name=MsG0280006834.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|86
Chr2 exon 6872646 6872906 6872646 ID=MsG0280006834.01.T01:exon:2904;Parent=MsG0280006834.01.T01
Chr2 CDS 6872646 6872906 6872646 ID=MsG0280006834.01.T01:cds;Parent=MsG0280006834.01.T01
Gene Sequence

>MsG0280006834.01.T01

ATGGCTGAAGTGAGAAAAGGTGAAGAGATACAAGGGATTAAGCTATTTGGCACAACAATAAAATTGCATGAGGAATTAAAAGAAGGTGAAAAAGAAAGTGAAGATCAAACGGTGGTGAAGAAGATAGAAAAGATCATACCATGTCCAAGATGCAAGAGTATGGAAACTAAATTTTGTTATTTCAATAATTATAACGTTAATCAACCAAGACATTTTTGTAAGAGCTGTCAAAGATACTGGACGGCTGGGGGCCCTCCGTAA

Protein sequence

>MsG0280006834.01.T01

MAEVRKGEEIQGIKLFGTTIKLHEELKEGEKESEDQTVVKKIEKIIPCPRCKSMETKFCYFNNYNVNQPRHFCKSCQRYWTAGGPP*