AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006882.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006882.01.T01 MTR_2g016020 97.619 168 4 0 1 168 1 168 2.65e-120 336
MsG0280006882.01.T01 MTR_2g077940 35.928 167 88 4 8 161 11 171 2.90e-27 100
MsG0280006882.01.T01 MTR_2g077960 36.025 161 90 4 8 161 11 165 4.64e-27 100
MsG0280006882.01.T01 MTR_3g087540 34.091 132 79 5 28 155 25 152 7.12e-22 86.7
MsG0280006882.01.T01 MTR_3g073990 32.911 158 102 2 1 157 1 155 1.32e-21 86.3
MsG0280006882.01.T01 MTR_0390s0010 35.032 157 92 2 8 161 11 160 2.94e-19 80.1
MsG0280006882.01.T01 MTR_0786s0020 34.395 157 93 2 8 161 11 160 2.06e-18 77.8
MsG0280006882.01.T01 MTR_2g450190 33.758 157 91 3 8 161 11 157 3.57e-16 72.0
MsG0280006882.01.T01 MTR_0390s0040 33.758 157 91 3 8 161 11 157 3.57e-16 72.0
MsG0280006882.01.T01 MTR_2g077080 31.915 141 87 2 23 161 31 164 4.88e-14 66.2
MsG0280006882.01.T01 MTR_1678s0010 28.082 146 95 5 28 168 38 178 1.40e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006882.01.T01 AT1G29140 49.412 170 80 4 1 164 1 170 2.35e-50 159
MsG0280006882.01.T01 AT5G45880 53.571 140 63 2 27 164 34 173 9.33e-50 158
MsG0280006882.01.T01 AT4G18596 47.953 171 82 4 1 164 1 171 1.20e-49 158
MsG0280006882.01.T01 AT4G18596 47.953 171 82 4 1 164 47 217 6.61e-49 157
MsG0280006882.01.T01 AT4G08685 39.241 158 89 5 8 162 6 159 1.95e-31 111
MsG0280006882.01.T01 AT5G10130 37.349 166 94 3 1 162 1 160 6.85e-28 102
MsG0280006882.01.T01 AT1G78040 32.143 168 103 5 1 162 1 163 4.43e-21 85.1
MsG0280006882.01.T01 AT1G78040 32.143 168 103 5 1 162 1 163 4.43e-21 85.1
MsG0280006882.01.T01 AT1G78040 32.143 168 103 5 1 162 1 163 4.43e-21 85.1

Find 66 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGCCCACATTAATAATT+AGG 0.085387 2:+7547396 MsG0280006882.01.T01:three_prime_UTR
GATTCATCCCAATTTGTCTT+AGG 0.203921 2:+7547550 MsG0280006882.01.T01:three_prime_UTR
GGATCCTTAACCAATGTCTT+TGG 0.250333 2:-7547590 None:intergenic
TAGCAAACCCTAAGACAAAT+TGG 0.255500 2:-7547558 None:intergenic
TTTGCCCACATTAATAATTA+GGG 0.297295 2:+7547397 MsG0280006882.01.T01:three_prime_UTR
TTGAGAACCTCACCACATTT+AGG 0.300765 2:-7547312 None:intergenic
AAGACAAAATTTATACTAAT+AGG 0.306921 2:-7547611 None:intergenic
TTCTTATGTGGGATTAATAA+TGG 0.307360 2:+7547420 MsG0280006882.01.T01:three_prime_UTR
CGGTCAATCTGGTGATGAAT+TGG 0.316647 2:-7546630 None:intergenic
GCCATTGTTCTTGATATTGT+TGG 0.323757 2:-7546490 None:intergenic
GTGGAATGCAAAGAAGAAAA+TGG 0.335509 2:+7547042 MsG0280006882.01.T01:CDS
GAAAATTAATTTACTTCTAA+AGG 0.352708 2:-7546456 None:intergenic
AGAGGGCTAGCAGCACGAAC+TGG 0.354642 2:-7547264 None:intergenic
CCTGAACGCTTCTTTGTAGA+AGG 0.366196 2:+7546580 MsG0280006882.01.T01:CDS
ATTCATCCCAATTTGTCTTA+GGG 0.388089 2:+7547551 MsG0280006882.01.T01:three_prime_UTR
AAATACAGTGTGGAGATAGA+TGG 0.398924 2:+7547108 MsG0280006882.01.T01:CDS
GTTCGTGCTGCTAGCCCTCT+TGG 0.402448 2:+7547267 MsG0280006882.01.T01:CDS
GATCCTTAACCAATGTCTTT+GGG 0.404663 2:-7547589 None:intergenic
TAGGTAGACGCTCTTTCTTA+AGG 0.405456 2:-7547293 None:intergenic
TGATCCTAGCAGCTTGTTGT+AGG 0.405713 2:-7547212 None:intergenic
TTCAATGAACTCGGTCAATC+TGG 0.407937 2:-7546641 None:intergenic
TAATAATTAGGGTTCTTATG+TGG 0.408457 2:+7547408 MsG0280006882.01.T01:three_prime_UTR
TATCCCAAAGACATTGGTTA+AGG 0.425381 2:+7547586 MsG0280006882.01.T01:three_prime_UTR
TACAAAGAAGCGTTCAGGCT+TGG 0.439377 2:-7546575 None:intergenic
GAACTTGGAATTAATGAAGA+TGG 0.440677 2:+7547336 MsG0280006882.01.T01:CDS
ACCTCTGAGCAATCTTTCCT+TGG 0.443518 2:-7547174 None:intergenic
AATAATTAGGGTTCTTATGT+GGG 0.452188 2:+7547409 MsG0280006882.01.T01:three_prime_UTR
GAAGCAACAACAGATAAATC+AGG 0.454577 2:+7547084 MsG0280006882.01.T01:CDS
TAATGAAGATGGAACTGAAA+AGG 0.471543 2:+7547347 MsG0280006882.01.T01:CDS
AATGAAAGGAATATCCTTTG+AGG 0.471700 2:-7547491 None:intergenic
CCTTCTACAAAGAAGCGTTC+AGG 0.474728 2:-7546580 None:intergenic
GGAATAAGCCGCACCTAAGA+AGG 0.479861 2:-7546554 None:intergenic
AGCAAACCCTAAGACAAATT+GGG 0.491288 2:-7547557 None:intergenic
GAGGGCTAGCAGCACGAACT+GGG 0.491624 2:-7547263 None:intergenic
AGATTGACCGAGTTCATTGA+AGG 0.500832 2:+7546643 MsG0280006882.01.T01:CDS
GGTGAGGTTCTCAAAGAACT+TGG 0.517638 2:+7547321 MsG0280006882.01.T01:CDS
GGTCAATCTGGTGATGAATT+GGG 0.518245 2:-7546629 None:intergenic
ATGAAAGGAATATCCTTTGA+GGG 0.520779 2:-7547490 None:intergenic
AAGAACCCTAATTATTAATG+TGG 0.527186 2:-7547402 None:intergenic
CTTCACATGTTTCATCCTCA+TGG 0.533134 2:-7547134 None:intergenic
TTATTCTTTGCAAGAGTCCA+AGG 0.534437 2:+7547157 MsG0280006882.01.T01:CDS
CTAGATTTCATGACCCTCAA+AGG 0.544332 2:+7547477 MsG0280006882.01.T01:three_prime_UTR
TAATGTGGGCAAAGGACAGA+AGG 0.550685 2:-7547387 None:intergenic
AAAAGCAAAGGGTGAAGACA+AGG 0.570670 2:-7546528 None:intergenic
AATTAGTATCCCAAAGACAT+TGG 0.576377 2:+7547580 MsG0280006882.01.T01:three_prime_UTR
CTAATTATTAATGTGGGCAA+AGG 0.581679 2:-7547395 None:intergenic
AGAACCCTAATTATTAATGT+GGG 0.587732 2:-7547401 None:intergenic
TGAAGGTGCAACGGTGCGTG+TGG 0.602260 2:+7547023 MsG0280006882.01.T01:intron
ATCTAGTATAACTCCTCCAA+AGG 0.603577 2:-7547460 None:intergenic
CTTTCTTAAGGAAACCAAGA+GGG 0.608256 2:-7547281 None:intergenic
ACCAACAATATCAAGAACAA+TGG 0.613722 2:+7546489 MsG0280006882.01.T01:exon
ATCAGTGTCACAAACAACAA+TGG 0.613848 2:+7547231 MsG0280006882.01.T01:CDS
AAGCAAAGGGTGAAGACAAG+GGG 0.617019 2:-7546526 None:intergenic
CTCACCTACAACAAGCTGCT+AGG 0.622703 2:+7547208 MsG0280006882.01.T01:CDS
TGAAGATGGAACTGAAAAGG+AGG 0.627791 2:+7547350 MsG0280006882.01.T01:CDS
GTACGTACCTTCAATGAACT+CGG 0.638216 2:-7546650 None:intergenic
AAAGAGCGTCTACCTAAATG+TGG 0.642536 2:+7547300 MsG0280006882.01.T01:CDS
AGGGCTAGCAGCACGAACTG+GGG 0.655258 2:-7547262 None:intergenic
TATATGTAATGAAGGTGCAA+CGG 0.656510 2:+7547014 MsG0280006882.01.T01:intron
AAAGCAAAGGGTGAAGACAA+GGG 0.660463 2:-7546527 None:intergenic
GCGTCTACCTAAATGTGGTG+AGG 0.675591 2:+7547305 MsG0280006882.01.T01:CDS
TCTTTCTTAAGGAAACCAAG+AGG 0.685975 2:-7547282 None:intergenic
TAAATCAGGAAAATACAGTG+TGG 0.693438 2:+7547098 MsG0280006882.01.T01:CDS
TCCAAGGAAAGATTGCTCAG+AGG 0.698789 2:+7547173 MsG0280006882.01.T01:CDS
AGCAAAGGGTGAAGACAAGG+GGG 0.721824 2:-7546525 None:intergenic
GGAGATAGATGGAGACCATG+AGG 0.745188 2:+7547119 MsG0280006882.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGACAAAATTTATACTAAT+AGG - Chr2:7547614-7547633 None:intergenic 15.0%
!! GAAAATTAATTTACTTCTAA+AGG - Chr2:7546459-7546478 None:intergenic 15.0%
!!! ATAATCTGAATTTTACTTTT+AGG - Chr2:7546854-7546873 None:intergenic 15.0%
!!! GACATAAATAATAATTTTTG+TGG + Chr2:7546988-7547007 MsG0280006882.01.T01:intron 15.0%
!! CTAGAGAAAATAAAAATGAA+AGG - Chr2:7547508-7547527 None:intergenic 20.0%
! AAGAACCCTAATTATTAATG+TGG - Chr2:7547405-7547424 None:intergenic 25.0%
! AATAATTAGGGTTCTTATGT+GGG + Chr2:7547409-7547428 MsG0280006882.01.T01:three_prime_UTR 25.0%
! ACACATTTATTACTAAGAAG+CGG + Chr2:7546914-7546933 MsG0280006882.01.T01:intron 25.0%
! AGAACCCTAATTATTAATGT+GGG - Chr2:7547404-7547423 None:intergenic 25.0%
! TAAGAAGGATAAAAAGCAAA+GGG - Chr2:7546542-7546561 None:intergenic 25.0%
! TAATAATTAGGGTTCTTATG+TGG + Chr2:7547408-7547427 MsG0280006882.01.T01:three_prime_UTR 25.0%
! TTCTTATGTGGGATTAATAA+TGG + Chr2:7547420-7547439 MsG0280006882.01.T01:three_prime_UTR 25.0%
! TTTGCCCACATTAATAATTA+GGG + Chr2:7547397-7547416 MsG0280006882.01.T01:three_prime_UTR 25.0%
!!! TTTTACTTTTAGGTATTACG+AGG - Chr2:7546844-7546863 None:intergenic 25.0%
AATGAAAGGAATATCCTTTG+AGG - Chr2:7547494-7547513 None:intergenic 30.0%
AATTAGTATCCCAAAGACAT+TGG + Chr2:7547580-7547599 MsG0280006882.01.T01:three_prime_UTR 30.0%
ACCAACAATATCAAGAACAA+TGG + Chr2:7546489-7546508 MsG0280006882.01.T01:exon 30.0%
ATGAAAGGAATATCCTTTGA+GGG - Chr2:7547493-7547512 None:intergenic 30.0%
ATTCATCCCAATTTGTCTTA+GGG + Chr2:7547551-7547570 MsG0280006882.01.T01:three_prime_UTR 30.0%
CTAAGAAGGATAAAAAGCAA+AGG - Chr2:7546543-7546562 None:intergenic 30.0%
CTAATTATTAATGTGGGCAA+AGG - Chr2:7547398-7547417 None:intergenic 30.0%
CTTTGCCCACATTAATAATT+AGG + Chr2:7547396-7547415 MsG0280006882.01.T01:three_prime_UTR 30.0%
GAACTTGGAATTAATGAAGA+TGG + Chr2:7547336-7547355 MsG0280006882.01.T01:CDS 30.0%
TAAATCAGGAAAATACAGTG+TGG + Chr2:7547098-7547117 MsG0280006882.01.T01:CDS 30.0%
TAATGAAGATGGAACTGAAA+AGG + Chr2:7547347-7547366 MsG0280006882.01.T01:CDS 30.0%
TTGCACCTTCATTACATATA+TGG - Chr2:7547014-7547033 None:intergenic 30.0%
! AGCTTTTATGTATGTACCTT+TGG + Chr2:7547444-7547463 MsG0280006882.01.T01:three_prime_UTR 30.0%
! TATATGTAATGAAGGTGCAA+CGG + Chr2:7547014-7547033 MsG0280006882.01.T01:intron 30.0%
! TTTTATGTATGTACCTTTGG+AGG + Chr2:7547447-7547466 MsG0280006882.01.T01:three_prime_UTR 30.0%
!!! CTTTGCTTTTTATCCTTCTT+AGG + Chr2:7546541-7546560 MsG0280006882.01.T01:CDS 30.0%
AAATACAGTGTGGAGATAGA+TGG + Chr2:7547108-7547127 MsG0280006882.01.T01:CDS 35.0%
ACACGATACATATTTGACAC+CGG - Chr2:7546730-7546749 None:intergenic 35.0%
AGCAAACCCTAAGACAAATT+GGG - Chr2:7547560-7547579 None:intergenic 35.0%
ATATATGTGACAGTGTGTGT+TGG - Chr2:7546759-7546778 None:intergenic 35.0%
ATCAGTGTCACAAACAACAA+TGG + Chr2:7547231-7547250 MsG0280006882.01.T01:CDS 35.0%
ATCTAGTATAACTCCTCCAA+AGG - Chr2:7547463-7547482 None:intergenic 35.0%
CTTTCTTAAGGAAACCAAGA+GGG - Chr2:7547284-7547303 None:intergenic 35.0%
GAAGCAACAACAGATAAATC+AGG + Chr2:7547084-7547103 MsG0280006882.01.T01:CDS 35.0%
GATCCTTAACCAATGTCTTT+GGG - Chr2:7547592-7547611 None:intergenic 35.0%
GATTCATCCCAATTTGTCTT+AGG + Chr2:7547550-7547569 MsG0280006882.01.T01:three_prime_UTR 35.0%
GCCATTGTTCTTGATATTGT+TGG - Chr2:7546493-7546512 None:intergenic 35.0%
GTGGAATGCAAAGAAGAAAA+TGG + Chr2:7547042-7547061 MsG0280006882.01.T01:CDS 35.0%
TAGCAAACCCTAAGACAAAT+TGG - Chr2:7547561-7547580 None:intergenic 35.0%
TCTTTCTTAAGGAAACCAAG+AGG - Chr2:7547285-7547304 None:intergenic 35.0%
TGTGGCCATATATGTAATGA+AGG + Chr2:7547006-7547025 MsG0280006882.01.T01:intron 35.0%
TTATTCTTTGCAAGAGTCCA+AGG + Chr2:7547157-7547176 MsG0280006882.01.T01:CDS 35.0%
! CTTTTTATCCTTCTTAGGTG+CGG + Chr2:7546546-7546565 MsG0280006882.01.T01:CDS 35.0%
! TATCCCAAAGACATTGGTTA+AGG + Chr2:7547586-7547605 MsG0280006882.01.T01:three_prime_UTR 35.0%
AAAAGCAAAGGGTGAAGACA+AGG - Chr2:7546531-7546550 None:intergenic 40.0%
AAAGCAAAGGGTGAAGACAA+GGG - Chr2:7546530-7546549 None:intergenic 40.0%
AGATTGACCGAGTTCATTGA+AGG + Chr2:7546643-7546662 MsG0280006882.01.T01:CDS 40.0%
CTAGATTTCATGACCCTCAA+AGG + Chr2:7547477-7547496 MsG0280006882.01.T01:three_prime_UTR 40.0%
CTTCACATGTTTCATCCTCA+TGG - Chr2:7547137-7547156 None:intergenic 40.0%
GCAAGACATTCGATATGCTA+AGG - Chr2:7546963-7546982 None:intergenic 40.0%
GGATCCTTAACCAATGTCTT+TGG - Chr2:7547593-7547612 None:intergenic 40.0%
GTACGTACCTTCAATGAACT+CGG - Chr2:7546653-7546672 None:intergenic 40.0%
TAGGTAGACGCTCTTTCTTA+AGG - Chr2:7547296-7547315 None:intergenic 40.0%
TATACTCCTCGAATTATGCG+TGG + Chr2:7546703-7546722 MsG0280006882.01.T01:intron 40.0%
TGAAGATGGAACTGAAAAGG+AGG + Chr2:7547350-7547369 MsG0280006882.01.T01:CDS 40.0%
TTCAATGAACTCGGTCAATC+TGG - Chr2:7546644-7546663 None:intergenic 40.0%
TTGAGAACCTCACCACATTT+AGG - Chr2:7547315-7547334 None:intergenic 40.0%
! GGTCAATCTGGTGATGAATT+GGG - Chr2:7546632-7546651 None:intergenic 40.0%
!! AAAGAGCGTCTACCTAAATG+TGG + Chr2:7547300-7547319 MsG0280006882.01.T01:CDS 40.0%
AAGCAAAGGGTGAAGACAAG+GGG - Chr2:7546529-7546548 None:intergenic 45.0%
ACCTCTGAGCAATCTTTCCT+TGG - Chr2:7547177-7547196 None:intergenic 45.0%
CCTGAACGCTTCTTTGTAGA+AGG + Chr2:7546580-7546599 MsG0280006882.01.T01:CDS 45.0%
GGTGAGGTTCTCAAAGAACT+TGG + Chr2:7547321-7547340 MsG0280006882.01.T01:CDS 45.0%
TAATGTGGGCAAAGGACAGA+AGG - Chr2:7547390-7547409 None:intergenic 45.0%
TCCAAGGAAAGATTGCTCAG+AGG + Chr2:7547173-7547192 MsG0280006882.01.T01:CDS 45.0%
! CGGTCAATCTGGTGATGAAT+TGG - Chr2:7546633-7546652 None:intergenic 45.0%
!! CCTTCTACAAAGAAGCGTTC+AGG - Chr2:7546583-7546602 None:intergenic 45.0%
!! TACAAAGAAGCGTTCAGGCT+TGG - Chr2:7546578-7546597 None:intergenic 45.0%
!! TGATCCTAGCAGCTTGTTGT+AGG - Chr2:7547215-7547234 None:intergenic 45.0%
AGCAAAGGGTGAAGACAAGG+GGG - Chr2:7546528-7546547 None:intergenic 50.0%
CTCACCTACAACAAGCTGCT+AGG + Chr2:7547208-7547227 MsG0280006882.01.T01:CDS 50.0%
GCGTCTACCTAAATGTGGTG+AGG + Chr2:7547305-7547324 MsG0280006882.01.T01:CDS 50.0%
GGAATAAGCCGCACCTAAGA+AGG - Chr2:7546557-7546576 None:intergenic 50.0%
GGAGATAGATGGAGACCATG+AGG + Chr2:7547119-7547138 MsG0280006882.01.T01:CDS 50.0%
TCCTCGAATTATGCGTGGTC+CGG + Chr2:7546708-7546727 MsG0280006882.01.T01:intron 50.0%
ACCGGACCACGCATAATTCG+AGG - Chr2:7546712-7546731 None:intergenic 55.0%
! AGAGGGCTAGCAGCACGAAC+TGG - Chr2:7547267-7547286 None:intergenic 60.0%
! AGGGCTAGCAGCACGAACTG+GGG - Chr2:7547265-7547284 None:intergenic 60.0%
! GAGGGCTAGCAGCACGAACT+GGG - Chr2:7547266-7547285 None:intergenic 60.0%
! GTTCGTGCTGCTAGCCCTCT+TGG + Chr2:7547267-7547286 MsG0280006882.01.T01:CDS 60.0%
! TGAAGGTGCAACGGTGCGTG+TGG + Chr2:7547023-7547042 MsG0280006882.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 7546449 7547693 7546449 ID=MsG0280006882.01;Name=MsG0280006882.01
Chr2 mRNA 7546449 7547693 7546449 ID=MsG0280006882.01.T01;Parent=MsG0280006882.01;Name=MsG0280006882.01.T01;_AED=0.49;_eAED=0.49;_QI=59|1|1|1|1|1|2|316|168
Chr2 exon 7546449 7546664 7546449 ID=MsG0280006882.01.T01:exon:2207;Parent=MsG0280006882.01.T01
Chr2 exon 7547028 7547693 7547028 ID=MsG0280006882.01.T01:exon:2208;Parent=MsG0280006882.01.T01
Chr2 five_prime_UTR 7546449 7546507 7546449 ID=MsG0280006882.01.T01:five_prime_utr;Parent=MsG0280006882.01.T01
Chr2 CDS 7546508 7546664 7546508 ID=MsG0280006882.01.T01:cds;Parent=MsG0280006882.01.T01
Chr2 CDS 7547028 7547377 7547028 ID=MsG0280006882.01.T01:cds;Parent=MsG0280006882.01.T01
Chr2 three_prime_UTR 7547378 7547693 7547378 ID=MsG0280006882.01.T01:three_prime_utr;Parent=MsG0280006882.01.T01
Gene Sequence

>MsG0280006882.01.T01

ATGGCAAAGTCTACAATCCCCCTTGTCTTCACCCTTTGCTTTTTATCCTTCTTAGGTGCGGCTTATTCCAAGCCTGAACGCTTCTTTGTAGAAGGTGTTGTTTACTGTGACACATGTCGCACCCAATTCATCACCAGATTGACCGAGTTCATTGAAGGTGCAACGGTGCGTGTGGAATGCAAAGAAGAAAATGGAACATTGACATTCAACAAAGAAGCAACAACAGATAAATCAGGAAAATACAGTGTGGAGATAGATGGAGACCATGAGGATGAAACATGTGAAGTTATTCTTTGCAAGAGTCCAAGGAAAGATTGCTCAGAGGTTGACTCAGAAGCTCACCTACAACAAGCTGCTAGGATCAGTGTCACAAACAACAATGGAATTGTGTCCCCAGTTCGTGCTGCTAGCCCTCTTGGTTTCCTTAAGAAAGAGCGTCTACCTAAATGTGGTGAGGTTCTCAAAGAACTTGGAATTAATGAAGATGGAACTGAAAAGGAGGACTAG

Protein sequence

>MsG0280006882.01.T01

MAKSTIPLVFTLCFLSFLGAAYSKPERFFVEGVVYCDTCRTQFITRLTEFIEGATVRVECKEENGTLTFNKEATTDKSGKYSVEIDGDHEDETCEVILCKSPRKDCSEVDSEAHLQQAARISVTNNNGIVSPVRAASPLGFLKKERLPKCGEVLKELGINEDGTEKED*