AlfalfaGEDB | MsG0280006886.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006886.01

KEGG: -
GO: -
PFAM: PF04852

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006886.01.T01	XP_013462558.1	98.913	184	2	0	1	184	1	184	5.62E-134	382

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006886.01.T01	Q9S7R3	71.519	158	35	2	1	158	1	148	1.47E-79	237

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006886.01.T01	I3T9J9	98.913	184	2	0	1	184	1	184	2.69e-134	382

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005127.01	MsG0280006886.01	0.809380	1.947392e-50	1.141635e-47
MsG0180005146.01	MsG0280006886.01	0.803291	3.738877e-49	1.871739e-46
MsG0280006886.01	MsG0280011016.01	0.802015	6.854489e-49	3.321676e-46
MsG0280006886.01	MsG0380012875.01	0.816215	6.203678e-52	4.369226e-49
MsG0280006886.01	MsG0480022244.01	-0.804509	2.088255e-49	1.078629e-46
MsG0280006886.01	MsG0580027517.01	0.807706	4.433335e-50	2.486565e-47
MsG0280006886.01	MsG0780038399.01	0.803075	4.144114e-49	2.063079e-46
MsG0280006886.01	MsG0780041100.01	0.808019	3.804341e-50	2.151344e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006886.01.T01	MTR_2g016110	98.913	184	2	0	1	184	1	184	6.81e-138	382
MsG0280006886.01.T01	MTR_2g076400	71.598	169	47	1	8	176	7	174	3.26e-84	246
MsG0280006886.01.T01	MTR_2g092950	85.714	126	18	0	33	158	21	146	2.77e-83	243
MsG0280006886.01.T01	MTR_4g079930	81.538	130	24	0	29	158	23	152	3.38e-82	241
MsG0280006886.01.T01	MTR_5g072510	69.655	145	43	1	25	168	26	170	2.66e-76	228
MsG0280006886.01.T01	MTR_4g094428	69.784	139	42	0	27	165	38	176	2.19e-72	218
MsG0280006886.01.T01	MTR_8g086350	80.282	142	26	1	32	171	28	169	7.36e-70	210
MsG0280006886.01.T01	MTR_1g442860	75.000	124	31	0	35	158	65	188	7.04e-69	209
MsG0280006886.01.T01	MTR_1g075990	75.000	140	35	0	26	165	44	183	1.42e-68	207
MsG0280006886.01.T01	MTR_7g115700	60.390	154	60	1	12	164	4	157	1.06e-65	199
MsG0280006886.01.T01	MTR_3g031830	74.046	131	34	0	35	165	42	172	2.73e-64	197
MsG0280006886.01.T01	MTR_7g097030	75.758	132	32	0	34	165	63	194	1.71e-63	195
MsG0280006886.01.T01	MTR_1g069825	75.573	131	32	0	35	165	52	182	6.25e-63	194
MsG0280006886.01.T01	MTR_1g080210	72.581	124	34	0	35	158	40	163	2.33e-58	181
MsG0280006886.01.T01	MTR_1g114020	66.667	45	15	0	34	78	70	114	1.50e-14	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006886.01.T01	AT2G42610	71.519	158	35	2	1	158	1	148	1.50e-80	237
MsG0280006886.01.T01	AT2G42610	71.519	158	35	2	1	158	1	148	1.50e-80	237
MsG0280006886.01.T01	AT4G18610	76.984	126	28	1	34	158	36	161	1.37e-73	220
MsG0280006886.01.T01	AT1G78815	74.603	126	32	0	33	158	38	163	7.77e-73	218
MsG0280006886.01.T01	AT1G07090	66.216	148	47	1	21	165	14	161	1.63e-72	217
MsG0280006886.01.T01	AT5G58500	72.932	133	35	1	34	165	18	150	1.83e-71	214
MsG0280006886.01.T01	AT1G16910	64.583	144	49	1	33	176	21	162	9.78e-67	201
MsG0280006886.01.T01	AT2G31160	67.722	158	47	1	12	165	27	184	2.94e-65	200
MsG0280006886.01.T01	AT3G23290	78.571	126	27	0	33	158	46	171	4.01e-64	196
MsG0280006886.01.T01	AT5G28490	71.324	136	39	0	34	169	24	159	3.43e-61	188
MsG0280006886.01.T01	AT3G04510	70.000	130	39	0	35	164	33	162	2.75e-60	186

Find 50 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCGTTACTTAGATCAATT+TGG	0.223804	2:+7621824	MsG0280006886.01.T01:CDS
AAACGTGACTGGAACACTTT+TGG	0.282441	2:+7621731	MsG0280006886.01.T01:CDS
ATGAATCTCAAGAAGCTAAT+TGG	0.317938	2:-7622150	None:intergenic
GAAGAAGAGGAGTCAACTTA+AGG	0.371496	2:+7622096	MsG0280006886.01.T01:CDS
GAGAATAATCCGTTTGGAAC+CGG	0.391211	2:+7621998	MsG0280006886.01.T01:CDS
TGTTTACCTTCGTGAAGTTA+AGG	0.392744	2:+7622030	MsG0280006886.01.T01:CDS
GTTGATCGGAGCCGCCGGGC+CGG	0.405992	2:-7621661	None:intergenic
TTGTCCGTTGAGACAAGCTT+GGG	0.422201	2:+7621913	MsG0280006886.01.T01:CDS
TTGATCGGAGCCGCCGGGCC+GGG	0.425263	2:-7621660	None:intergenic
AAAGGAGCTACTGCCGTTGC+TGG	0.426810	2:-7621686	None:intergenic
AGATCAATTTGGAAAGACTA+AGG	0.447537	2:+7621835	MsG0280006886.01.T01:CDS
TTGCACTGAGACAAAGAAAC+TGG	0.458457	2:-7621779	None:intergenic
AAGAAGAGGAGTCAACTTAA+GGG	0.459586	2:+7622097	MsG0280006886.01.T01:CDS
GTACACGGTGCCGGCGGATC+CGG	0.461206	2:-7621890	None:intergenic
TGCCGTTGCTGGTTGTTGAT+CGG	0.471645	2:-7621675	None:intergenic
TCGCCGGAGAATAATCCGTT+TGG	0.482372	2:+7621992	MsG0280006886.01.T01:CDS
TGAAGAACACGGTGGATCGC+CGG	0.490859	2:+7621976	MsG0280006886.01.T01:CDS
TGGTTGTTGATCGGAGCCGC+CGG	0.503692	2:-7621666	None:intergenic
CGAATAGCTCCGGTTCCAAA+CGG	0.517509	2:-7622007	None:intergenic
TCATCTTCATCGTCCCGGCC+CGG	0.520026	2:+7621647	MsG0280006886.01.T01:CDS
TTTCGGACAACCGGATCCGC+CGG	0.521709	2:+7621880	MsG0280006886.01.T01:CDS
CGATGAAGATGAGGCTTGAA+CGG	0.529432	2:-7621636	None:intergenic
AAGACTAAGGTTCACTTGCA+CGG	0.541275	2:+7621848	MsG0280006886.01.T01:CDS
CTTGTCCGTTGAGACAAGCT+TGG	0.546352	2:+7621912	MsG0280006886.01.T01:CDS
AAGCCTCATCTTCATCGTCC+CGG	0.547057	2:+7621642	MsG0280006886.01.T01:CDS
GTTCCAAACGGATTATTCTC+CGG	0.548543	2:-7621995	None:intergenic
ACAAAGAAACTGGTGGTGAT+TGG	0.549062	2:-7621769	None:intergenic
GGTTGTTGATCGGAGCCGCC+GGG	0.549752	2:-7621665	None:intergenic
AACGGACAAGTACACGGTGC+CGG	0.553548	2:-7621899	None:intergenic
CTTCCCCAAGCTTGTCTCAA+CGG	0.565292	2:-7621917	None:intergenic
TGTGACTCGTAACGGCTCAA+AGG	0.580787	2:-7621704	None:intergenic
CTCCGATCAACAACCAGCAA+CGG	0.582443	2:+7621673	MsG0280006886.01.T01:CDS
GTCTTGATGCACTCATCGGA+CGG	0.586993	2:+7621939	MsG0280006886.01.T01:CDS
AAGGTAAACACGAATAGCTC+CGG	0.596382	2:-7622017	None:intergenic
AGTTAAGGAGTGTCAATCAA+AGG	0.600949	2:+7622045	MsG0280006886.01.T01:CDS
AGTGTCAATCAAAGGCTAGA+GGG	0.602927	2:+7622053	MsG0280006886.01.T01:CDS
AGTCACAAAAGAAACGTGAC+TGG	0.604650	2:+7621720	MsG0280006886.01.T01:CDS
GGAAGTCTTGATGCACTCAT+CGG	0.605369	2:+7621935	MsG0280006886.01.T01:CDS
CAAATTGATCTAAGTAACGA+AGG	0.614321	2:-7621823	None:intergenic
GGACAAGTACACGGTGCCGG+CGG	0.627407	2:-7621896	None:intergenic
GAGTGTCAATCAAAGGCTAG+AGG	0.627751	2:+7622052	MsG0280006886.01.T01:CDS
GGGCCGGGACGATGAAGATG+AGG	0.628323	2:-7621645	None:intergenic
GACACTCCTTAACTTCACGA+AGG	0.661380	2:-7622036	None:intergenic
TGTCTCAACGGACAAGTACA+CGG	0.662830	2:-7621905	None:intergenic
AACGAAGGAACTCAAGCACA+TGG	0.689469	2:-7621808	None:intergenic
TCTTCATCGTCCCGGCCCGG+CGG	0.699949	2:+7621650	MsG0280006886.01.T01:CDS
TGTCCGTTGAGACAAGCTTG+GGG	0.714712	2:+7621914	MsG0280006886.01.T01:CDS
AGAGCTGCTTATGAAGAACA+CGG	0.720741	2:+7621965	MsG0280006886.01.T01:CDS
CACTGAGACAAAGAAACTGG+TGG	0.739687	2:-7621776	None:intergenic
GCTGCTTATGAAGAACACGG+TGG	0.793205	2:+7621968	MsG0280006886.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTCTTTTTCTTTGTGTAT+GGG	-	Chr2:7622081-7622100	None:intergenic	25.0%
!!	TTCTTCTTTTTCTTTGTGTA+TGG	-	Chr2:7622082-7622101	None:intergenic	25.0%
	ACACAAAGAAAAAGAAGAAG+AGG	+	Chr2:7622083-7622102	MsG0280006886.01.T01:CDS	30.0%
	AGATCAATTTGGAAAGACTA+AGG	+	Chr2:7621835-7621854	MsG0280006886.01.T01:CDS	30.0%
	CAAATTGATCTAAGTAACGA+AGG	-	Chr2:7621826-7621845	None:intergenic	30.0%
	CTTCGTTACTTAGATCAATT+TGG	+	Chr2:7621824-7621843	MsG0280006886.01.T01:CDS	30.0%
!	ACACTTTTGGACAATACTTA+AGG	+	Chr2:7621744-7621763	MsG0280006886.01.T01:CDS	30.0%
	AAGAAGAGGAGTCAACTTAA+GGG	+	Chr2:7622097-7622116	MsG0280006886.01.T01:CDS	35.0%
	TGTTTACCTTCGTGAAGTTA+AGG	+	Chr2:7622030-7622049	MsG0280006886.01.T01:CDS	35.0%
!	GTTTCTTTTGTGACTCGTAA+CGG	-	Chr2:7621715-7621734	None:intergenic	35.0%
!!	AGTTAAGGAGTGTCAATCAA+AGG	+	Chr2:7622045-7622064	MsG0280006886.01.T01:CDS	35.0%
	AAGACTAAGGTTCACTTGCA+CGG	+	Chr2:7621848-7621867	MsG0280006886.01.T01:CDS	40.0%
	AAGGTAAACACGAATAGCTC+CGG	-	Chr2:7622020-7622039	None:intergenic	40.0%
	AGAGCTGCTTATGAAGAACA+CGG	+	Chr2:7621965-7621984	MsG0280006886.01.T01:CDS	40.0%
	AGTCACAAAAGAAACGTGAC+TGG	+	Chr2:7621720-7621739	MsG0280006886.01.T01:CDS	40.0%
	AGTGTCAATCAAAGGCTAGA+GGG	+	Chr2:7622053-7622072	MsG0280006886.01.T01:CDS	40.0%
	GAAGAAGAGGAGTCAACTTA+AGG	+	Chr2:7622096-7622115	MsG0280006886.01.T01:CDS	40.0%
	TTGCACTGAGACAAAGAAAC+TGG	-	Chr2:7621782-7621801	None:intergenic	40.0%
!	AAACGTGACTGGAACACTTT+TGG	+	Chr2:7621731-7621750	MsG0280006886.01.T01:CDS	40.0%
!	ACAAAGAAACTGGTGGTGAT+TGG	-	Chr2:7621772-7621791	None:intergenic	40.0%
!	GAGAATAATCCGTTTGGAAC+CGG	+	Chr2:7621998-7622017	MsG0280006886.01.T01:CDS	40.0%
!	TTGCACGGTTGCATCTTTTT+CGG	+	Chr2:7621863-7621882	MsG0280006886.01.T01:CDS	40.0%
!!	GTTCCAAACGGATTATTCTC+CGG	-	Chr2:7621998-7622017	None:intergenic	40.0%
!!!	TTGCATCTTTTTCGGACAAC+CGG	+	Chr2:7621871-7621890	MsG0280006886.01.T01:CDS	40.0%
	AACGAAGGAACTCAAGCACA+TGG	-	Chr2:7621811-7621830	None:intergenic	45.0%
	CACTGAGACAAAGAAACTGG+TGG	-	Chr2:7621779-7621798	None:intergenic	45.0%
	CGATGAAGATGAGGCTTGAA+CGG	-	Chr2:7621639-7621658	None:intergenic	45.0%
	GACACTCCTTAACTTCACGA+AGG	-	Chr2:7622039-7622058	None:intergenic	45.0%
	GAGTGTCAATCAAAGGCTAG+AGG	+	Chr2:7622052-7622071	MsG0280006886.01.T01:CDS	45.0%
	GGAAGTCTTGATGCACTCAT+CGG	+	Chr2:7621935-7621954	MsG0280006886.01.T01:CDS	45.0%
	TGTCTCAACGGACAAGTACA+CGG	-	Chr2:7621908-7621927	None:intergenic	45.0%
	TTGTCCGTTGAGACAAGCTT+GGG	+	Chr2:7621913-7621932	MsG0280006886.01.T01:CDS	45.0%
	AAGCCTCATCTTCATCGTCC+CGG	+	Chr2:7621642-7621661	MsG0280006886.01.T01:CDS	50.0%
	CGAATAGCTCCGGTTCCAAA+CGG	-	Chr2:7622010-7622029	None:intergenic	50.0%
	CTCCGATCAACAACCAGCAA+CGG	+	Chr2:7621673-7621692	MsG0280006886.01.T01:CDS	50.0%
	CTTCCCCAAGCTTGTCTCAA+CGG	-	Chr2:7621920-7621939	None:intergenic	50.0%
	CTTGTCCGTTGAGACAAGCT+TGG	+	Chr2:7621912-7621931	MsG0280006886.01.T01:CDS	50.0%
	GCTGCTTATGAAGAACACGG+TGG	+	Chr2:7621968-7621987	MsG0280006886.01.T01:CDS	50.0%
	GTCTTGATGCACTCATCGGA+CGG	+	Chr2:7621939-7621958	MsG0280006886.01.T01:CDS	50.0%
	TCGCCGGAGAATAATCCGTT+TGG	+	Chr2:7621992-7622011	MsG0280006886.01.T01:CDS	50.0%
	TGTCCGTTGAGACAAGCTTG+GGG	+	Chr2:7621914-7621933	MsG0280006886.01.T01:CDS	50.0%
	TGTGACTCGTAACGGCTCAA+AGG	-	Chr2:7621707-7621726	None:intergenic	50.0%
!	TGCCGTTGCTGGTTGTTGAT+CGG	-	Chr2:7621678-7621697	None:intergenic	50.0%
	AAAGGAGCTACTGCCGTTGC+TGG	-	Chr2:7621689-7621708	None:intergenic	55.0%
	AACGGACAAGTACACGGTGC+CGG	-	Chr2:7621902-7621921	None:intergenic	55.0%
	TGAAGAACACGGTGGATCGC+CGG	+	Chr2:7621976-7621995	MsG0280006886.01.T01:CDS	55.0%
	TCATCTTCATCGTCCCGGCC+CGG	+	Chr2:7621647-7621666	MsG0280006886.01.T01:CDS	60.0%
	TGGTTGTTGATCGGAGCCGC+CGG	-	Chr2:7621669-7621688	None:intergenic	60.0%
	TTTCGGACAACCGGATCCGC+CGG	+	Chr2:7621880-7621899	MsG0280006886.01.T01:CDS	60.0%
	GGGCCGGGACGATGAAGATG+AGG	-	Chr2:7621648-7621667	None:intergenic	65.0%
	GGTTGTTGATCGGAGCCGCC+GGG	-	Chr2:7621668-7621687	None:intergenic	65.0%
!	GGACAAGTACACGGTGCCGG+CGG	-	Chr2:7621899-7621918	None:intergenic	65.0%
	TCTTCATCGTCCCGGCCCGG+CGG	+	Chr2:7621650-7621669	MsG0280006886.01.T01:CDS	70.0%
!!	GTACACGGTGCCGGCGGATC+CGG	-	Chr2:7621893-7621912	None:intergenic	70.0%
	GTTGATCGGAGCCGCCGGGC+CGG	-	Chr2:7621664-7621683	None:intergenic	75.0%
	TTGATCGGAGCCGCCGGGCC+GGG	-	Chr2:7621663-7621682	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	7621611	7622165	7621611	ID=MsG0280006886.01;Name=MsG0280006886.01
Chr2	mRNA	7621611	7622165	7621611	ID=MsG0280006886.01.T01;Parent=MsG0280006886.01;Name=MsG0280006886.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|184
Chr2	exon	7621611	7622165	7621611	ID=MsG0280006886.01.T01:exon:2215;Parent=MsG0280006886.01.T01
Chr2	CDS	7621611	7622165	7621611	ID=MsG0280006886.01.T01:cds;Parent=MsG0280006886.01.T01

Gene Sequence

>MsG0280006886.01.T01

ATGTCAAGCTCAATGCAAAACGACACCGTTCAAGCCTCATCTTCATCGTCCCGGCCCGGCGGCTCCGATCAACAACCAGCAACGGCAGTAGCTCCTTTGAGCCGTTACGAGTCACAAAAGAAACGTGACTGGAACACTTTTGGACAATACTTAAGGAACCAATCACCACCAGTTTCTTTGTCTCAGTGCAACTTCAACCATGTGCTTGAGTTCCTTCGTTACTTAGATCAATTTGGAAAGACTAAGGTTCACTTGCACGGTTGCATCTTTTTCGGACAACCGGATCCGCCGGCACCGTGTACTTGTCCGTTGAGACAAGCTTGGGGAAGTCTTGATGCACTCATCGGACGGCTAAGAGCTGCTTATGAAGAACACGGTGGATCGCCGGAGAATAATCCGTTTGGAACCGGAGCTATTCGTGTTTACCTTCGTGAAGTTAAGGAGTGTCAATCAAAGGCTAGAGGGATCCCATACACAAAGAAAAAGAAGAAGAGGAGTCAACTTAAGGGCACTCATAATACTACTTCTAAGTCTTTCAACCAATTAGCTTCTTGA

Protein sequence

>MsG0280006886.01.T01

MSSSMQNDTVQASSSSSRPGGSDQQPATAVAPLSRYESQKKRDWNTFGQYLRNQSPPVSLSQCNFNHVLEFLRYLDQFGKTKVHLHGCIFFGQPDPPAPCTCPLRQAWGSLDALIGRLRAAYEEHGGSPENNPFGTGAIRVYLREVKECQSKARGIPYTKKKKKRSQLKGTHNTTSKSFNQLAS*