AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048856.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048856.01.T01 MTR_7g056133 98.969 97 1 0 1 97 122 218 1.62e-60 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048856.01.T01 AT3G07190 67.033 91 30 0 1 91 122 212 6.35e-15 67.4
MsG0080048856.01.T01 AT5G48660 67.347 98 31 1 1 97 46 143 1.21e-14 65.1
MsG0080048856.01.T01 AT5G48660 67.347 98 31 1 1 97 122 219 3.34e-14 65.5
MsG0080048856.01.T01 AT5G42570 39.080 87 53 0 5 91 118 204 1.29e-11 58.5

Find 15 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTGAGCCTGAAGATTT+TGG 0.137576 contig490end:+31723 MsG0080048856.01.T01:intergenic
AACTCTCTTGTCTTTCTCTT+CGG 0.340250 contig490end:+31829 MsG0080048856.01.T01:intergenic
TCTAACAGAAGGTTTGAAAA+AGG 0.345623 contig490end:-31864 MsG0080048856.01.T01:CDS
GGAAGACAACCAAAATCTTC+AGG 0.401724 contig490end:-31732 MsG0080048856.01.T01:CDS
CAAAATCTTCAGGCTCAAGC+AGG 0.486517 contig490end:-31722 MsG0080048856.01.T01:CDS
AAATGTCGAGCTCAAAGAAA+AGG 0.491750 contig490end:-31936 MsG0080048856.01.T01:CDS
GCTTGAATATGACAGACTCT+TGG 0.508233 contig490end:-31753 MsG0080048856.01.T01:CDS
GAACTTTCAACTCTAACAGA+AGG 0.533214 contig490end:-31875 MsG0080048856.01.T01:CDS
AAAGGAGGAGAAAGCTTCCA+AGG 0.549039 contig490end:-31918 MsG0080048856.01.T01:CDS
GGGAGCTTCAAAAGAAGAAG+TGG 0.560241 contig490end:-31975 MsG0080048856.01.T01:CDS
GAAAGACAAGAGAGTTGAGA+CGG 0.586053 contig490end:-31822 MsG0080048856.01.T01:CDS
GCAGGGAGGCATAAGAGTTG+AGG 0.597943 contig490end:-31704 None:intergenic
AAAATCTTCAGGCTCAAGCA+GGG 0.625078 contig490end:-31721 MsG0080048856.01.T01:CDS
TGTCGAGCTCAAAGAAAAGG+AGG 0.645126 contig490end:-31933 MsG0080048856.01.T01:CDS
ATCTTCAGGCTCAAGCAGGG+AGG 0.695143 contig490end:-31718 MsG0080048856.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGTTTGAAAAAGGTAAAGA+TGG - contig490end:31828-31847 MsG0080048856.01.T01:CDS 30.0%
TCTAACAGAAGGTTTGAAAA+AGG - contig490end:31819-31838 MsG0080048856.01.T01:CDS 30.0%
AAATGTCGAGCTCAAAGAAA+AGG - contig490end:31747-31766 MsG0080048856.01.T01:CDS 35.0%
AACTCTCTTGTCTTTCTCTT+CGG + contig490end:31857-31876 MsG0080048856.01.T01:intergenic 35.0%
GAACTTTCAACTCTAACAGA+AGG - contig490end:31808-31827 MsG0080048856.01.T01:CDS 35.0%
!! GCTCGACATTTTCTTTTTTG+AGG + contig490end:31738-31757 MsG0080048856.01.T01:intergenic 35.0%
!! GTAAATCTGCAGCTTGTTTT+TGG + contig490end:31909-31928 MsG0080048856.01.T01:intergenic 35.0%
AAAATCTTCAGGCTCAAGCA+GGG - contig490end:31962-31981 MsG0080048856.01.T01:CDS 40.0%
GAAAGACAAGAGAGTTGAGA+CGG - contig490end:31861-31880 MsG0080048856.01.T01:CDS 40.0%
GCTTGAATATGACAGACTCT+TGG - contig490end:31930-31949 MsG0080048856.01.T01:CDS 40.0%
GGAAGACAACCAAAATCTTC+AGG - contig490end:31951-31970 MsG0080048856.01.T01:CDS 40.0%
!! CTTCAGCTGTTTTATCTCCT+TGG + contig490end:31785-31804 MsG0080048856.01.T01:intergenic 40.0%
!!! AATCTGCAGCTTGTTTTTGG+AGG + contig490end:31906-31925 MsG0080048856.01.T01:intergenic 40.0%
!!! ATCTGCAGCTTGTTTTTGGA+GGG + contig490end:31905-31924 MsG0080048856.01.T01:intergenic 40.0%
AAAGGAGGAGAAAGCTTCCA+AGG - contig490end:31765-31784 MsG0080048856.01.T01:CDS 45.0%
CAAAATCTTCAGGCTCAAGC+AGG - contig490end:31961-31980 MsG0080048856.01.T01:CDS 45.0%
GGGAGCTTCAAAAGAAGAAG+TGG - contig490end:31708-31727 MsG0080048856.01.T01:CDS 45.0%
TGTCGAGCTCAAAGAAAAGG+AGG - contig490end:31750-31769 MsG0080048856.01.T01:CDS 45.0%
! CTGCTTGAGCCTGAAGATTT+TGG + contig490end:31963-31982 MsG0080048856.01.T01:intergenic 45.0%
ATCTTCAGGCTCAAGCAGGG+AGG - contig490end:31965-31984 MsG0080048856.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
contig490end gene 31706 31999 31706 ID=MsG0080048856.01;Name=MsG0080048856.01
contig490end mRNA 31706 31999 31706 ID=MsG0080048856.01.T01;Parent=MsG0080048856.01;Name=MsG0080048856.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|97
contig490end exon 31706 31999 31706 ID=MsG0080048856.01.T01:exon:4852;Parent=MsG0080048856.01.T01
contig490end CDS 31706 31999 31706 ID=MsG0080048856.01.T01:cds;Parent=MsG0080048856.01.T01
Gene Sequence

>MsG0080048856.01.T01

ATGGGAGCTTCAAAAGAAGAAGTGGAGAACCTCAAAAAAGAAAATGTCGAGCTCAAAGAAAAGGAGGAGAAAGCTTCCAAGGAGATAAAACAGCTGAAGAAAGAACTTTCAACTCTAACAGAAGGTTTGAAAAAGGTAAAGATGGAATCCGAAGAGAAAGACAAGAGAGTTGAGACGGCTGATGCTCATGTTGCTTCCCTCCAAAAACAAGCTGCAGATTTACTGCTTGAATATGACAGACTCTTGGAAGACAACCAAAATCTTCAGGCTCAAGCAGGGAGGCATAAGAGTTGA

Protein sequence

>MsG0080048856.01.T01

MGASKEEVENLKKENVELKEKEEKASKEIKQLKKELSTLTEGLKKVKMESEEKDKRVETADAHVASLQKQAADLLLEYDRLLEDNQNLQAQAGRHKS*