Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007498.01.T01 | RHN72680.1 | 92.727 | 55 | 4 | 0 | 12 | 66 | 108 | 162 | 2.34E-27 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007498.01.T01 | A0A396J444 | 92.727 | 55 | 4 | 0 | 12 | 66 | 108 | 162 | 1.12e-27 | 106 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003350.01 | MsG0280007498.01 | 0.806419 | 8.303257e-50 | 4.504450e-47 |
| MsG0180004865.01 | MsG0280007498.01 | 0.803668 | 3.122965e-49 | 1.578620e-46 |
| MsG0280007498.01 | MsG0380015636.01 | 0.824473 | 7.918793e-54 | 7.020907e-51 |
| MsG0280007498.01 | MsG0480018658.01 | 0.807041 | 6.133644e-50 | 3.381287e-47 |
| MsG0280007498.01 | MsG0480022150.01 | 0.808715 | 2.702806e-50 | 1.556502e-47 |
| MsG0280007498.01 | MsG0480022411.01 | 0.803558 | 3.292109e-49 | 1.659583e-46 |
| MsG0280007498.01 | MsG0480022458.01 | 0.800073 | 1.709920e-48 | 7.888452e-46 |
| MsG0280007498.01 | MsG0480022935.01 | 0.801430 | 9.036973e-49 | 4.314811e-46 |
| MsG0280007498.01 | MsG0780037963.01 | 0.834929 | 2.255090e-56 | 2.715896e-53 |
| MsG0280007498.01 | MsG0780038030.01 | 0.812757 | 3.611131e-51 | 2.315334e-48 |
| MsG0280007498.01 | MsG0780038108.01 | 0.809143 | 2.188420e-50 | 1.274870e-47 |
| MsG0280007498.01 | MsG0780038756.01 | 0.800124 | 1.669468e-48 | 7.711868e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007498.01.T01 | MTR_2g026575 | 95.238 | 42 | 2 | 0 | 25 | 66 | 128 | 169 | 2.43e-23 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCCACTACTGTCTTCT+TGG | 0.158910 | 2:-16312167 | MsG0280007498.01.T01:CDS |
| TTGGTAGCAATTATAGCCTT+TGG | 0.333991 | 2:-16312148 | MsG0280007498.01.T01:CDS |
| TGAGACTCTTGAGTATGTTC+AGG | 0.412384 | 2:+16312049 | None:intergenic |
| AAAAGCTGGTGAAGGACTCT+TGG | 0.437845 | 2:-16312089 | MsG0280007498.01.T01:CDS |
| GGACTCTTGGATTATGTAGC+AGG | 0.481838 | 2:-16312076 | MsG0280007498.01.T01:CDS |
| GTAGCAATTATAGCCTTTGG+AGG | 0.486278 | 2:-16312145 | MsG0280007498.01.T01:CDS |
| AAATTCTCAACTGAGACTCA+TGG | 0.600519 | 2:-16312115 | MsG0280007498.01.T01:CDS |
| TGCTACCAAGAAGACAGTAG+TGG | 0.636834 | 2:+16312162 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGCAAAAAAGGCCAAAAAT+AGG | + | Chr2:16312068-16312087 | None:intergenic | 30.0% | |
| ! | TTACCATTCTAGCCTATTTT+TGG | - | Chr2:16312053-16312072 | MsG0280007498.01.T01:CDS | 30.0% |
| AAATTCTCAACTGAGACTCA+TGG | - | Chr2:16312135-16312154 | MsG0280007498.01.T01:CDS | 35.0% | |
| ! | TTGGTAGCAATTATAGCCTT+TGG | - | Chr2:16312102-16312121 | MsG0280007498.01.T01:CDS | 35.0% |
| !!! | GAATTTTGAAACACCTCCAA+AGG | + | Chr2:16312121-16312140 | None:intergenic | 35.0% |
| AGGCCAAAAATAGGCTAGAA+TGG | + | Chr2:16312059-16312078 | None:intergenic | 40.0% | |
| GGCTAGAATGGTAAAAAGTG+TGG | + | Chr2:16312047-16312066 | None:intergenic | 40.0% | |
| GTAGCAATTATAGCCTTTGG+AGG | - | Chr2:16312105-16312124 | MsG0280007498.01.T01:CDS | 40.0% | |
| TGAGACTCTTGAGTATGTTC+AGG | + | Chr2:16312204-16312223 | None:intergenic | 40.0% | |
| ! | GACAGTAGTGGAAGCAAAAA+AGG | + | Chr2:16312079-16312098 | None:intergenic | 40.0% |
| AAAAGCTGGTGAAGGACTCT+TGG | - | Chr2:16312161-16312180 | MsG0280007498.01.T01:CDS | 45.0% | |
| CATGGTGAAAAAGCTGGTGA+AGG | - | Chr2:16312153-16312172 | MsG0280007498.01.T01:CDS | 45.0% | |
| GAGACTCATGGTGAAAAAGC+TGG | - | Chr2:16312147-16312166 | MsG0280007498.01.T01:CDS | 45.0% | |
| GGACTCTTGGATTATGTAGC+AGG | - | Chr2:16312174-16312193 | MsG0280007498.01.T01:CDS | 45.0% | |
| TGCTACCAAGAAGACAGTAG+TGG | + | Chr2:16312091-16312110 | None:intergenic | 45.0% | |
| TGCTTCCACTACTGTCTTCT+TGG | - | Chr2:16312083-16312102 | MsG0280007498.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 16312036 | 16312236 | 16312036 | ID=MsG0280007498.01;Name=MsG0280007498.01 |
| Chr2 | mRNA | 16312036 | 16312236 | 16312036 | ID=MsG0280007498.01.T01;Parent=MsG0280007498.01;Name=MsG0280007498.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|66 |
| Chr2 | exon | 16312036 | 16312236 | 16312036 | ID=MsG0280007498.01.T01:exon:4331;Parent=MsG0280007498.01.T01 |
| Chr2 | CDS | 16312036 | 16312236 | 16312036 | ID=MsG0280007498.01.T01:cds;Parent=MsG0280007498.01.T01 |
| Gene Sequence |
| Protein sequence |
