Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007944.01.T01 | RHN73156.1 | 96.629 | 89 | 3 | 0 | 1 | 89 | 47 | 135 | 1.96E-57 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007944.01.T01 | A0A396J4Y6 | 96.629 | 89 | 3 | 0 | 1 | 89 | 47 | 135 | 9.37e-58 | 182 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004493.01 | MsG0280007944.01 | 0.800376 | 1.483495e-48 | 6.897015e-46 |
| MsG0280007944.01 | MsG0480021776.01 | -0.813706 | 2.235043e-51 | 1.470241e-48 |
| MsG0280007944.01 | MsG0480022248.01 | 0.804171 | 2.455176e-49 | 1.257235e-46 |
| MsG0280007944.01 | MsG0580025627.01 | 0.804035 | 2.620539e-49 | 1.337284e-46 |
| MsG0280007944.01 | MsG0580026206.01 | 0.808945 | 2.413386e-50 | 1.398369e-47 |
| MsG0280007944.01 | MsG0680030555.01 | 0.800627 | 1.319022e-48 | 6.171264e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007944.01.T01 | MTR_2g435650 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 2.91e-61 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATCAACTTCAGGTTAA+AGG | 0.182716 | 2:-23385372 | MsG0280007944.01.T01:CDS |
| TCAACTTCAGGTTAAAGGTT+TGG | 0.389188 | 2:-23385367 | MsG0280007944.01.T01:CDS |
| AACACTTGTTTGCACCACTT+TGG | 0.438820 | 2:+23385446 | None:intergenic |
| AAAGAGAAAAGATCAACTTC+AGG | 0.458185 | 2:-23385379 | MsG0280007944.01.T01:CDS |
| CTTCAGGTTAAAGGTTTGGT+TGG | 0.475441 | 2:-23385363 | MsG0280007944.01.T01:CDS |
| GGTAACAGTAGTAGCACCAT+AGG | 0.534911 | 2:+23385467 | None:intergenic |
| ACAGTAGTAGCACCATAGGT+TGG | 0.582294 | 2:+23385471 | None:intergenic |
| GCAATAGCAAAATCTGCTTG+AGG | 0.632778 | 2:+23385597 | None:intergenic |
| TGCTACTACTGTTACCAAAG+TGG | 0.670327 | 2:-23385460 | MsG0280007944.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGAAATTGAAAAATTCTT+TGG | - | Chr2:23385395-23385414 | MsG0280007944.01.T01:CDS | 15.0% |
| !! | TACAAATTTTATCCAACCTA+TGG | - | Chr2:23385476-23385495 | MsG0280007944.01.T01:CDS | 25.0% |
| AAAGAGAAAAGATCAACTTC+AGG | - | Chr2:23385580-23385599 | MsG0280007944.01.T01:CDS | 30.0% | |
| ! | AAAGATCAACTTCAGGTTAA+AGG | - | Chr2:23385587-23385606 | MsG0280007944.01.T01:CDS | 30.0% |
| !!! | GATTTTGCTATTGCTAAGAT+TGG | - | Chr2:23385371-23385390 | MsG0280007944.01.T01:CDS | 30.0% |
| ! | TCAACTTCAGGTTAAAGGTT+TGG | - | Chr2:23385592-23385611 | MsG0280007944.01.T01:CDS | 35.0% |
| !! | AAAGATTTTGAGTAGCTGGA+GGG | + | Chr2:23385451-23385470 | None:intergenic | 35.0% |
| !! | TGACAAAGATTTTGAGTAGC+TGG | + | Chr2:23385455-23385474 | None:intergenic | 35.0% |
| AACACTTGTTTGCACCACTT+TGG | + | Chr2:23385516-23385535 | None:intergenic | 40.0% | |
| GCAATAGCAAAATCTGCTTG+AGG | + | Chr2:23385365-23385384 | None:intergenic | 40.0% | |
| TGCTACTACTGTTACCAAAG+TGG | - | Chr2:23385499-23385518 | MsG0280007944.01.T01:CDS | 40.0% | |
| ! | CTTCAGGTTAAAGGTTTGGT+TGG | - | Chr2:23385596-23385615 | MsG0280007944.01.T01:CDS | 40.0% |
| !! | CAAAGATTTTGAGTAGCTGG+AGG | + | Chr2:23385452-23385471 | None:intergenic | 40.0% |
| ACAGTAGTAGCACCATAGGT+TGG | + | Chr2:23385491-23385510 | None:intergenic | 45.0% | |
| GGTAACAGTAGTAGCACCAT+AGG | + | Chr2:23385495-23385514 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 23385356 | 23385625 | 23385356 | ID=MsG0280007944.01;Name=MsG0280007944.01 |
| Chr2 | mRNA | 23385356 | 23385625 | 23385356 | ID=MsG0280007944.01.T01;Parent=MsG0280007944.01;Name=MsG0280007944.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr2 | exon | 23385356 | 23385625 | 23385356 | ID=MsG0280007944.01.T01:exon:2992;Parent=MsG0280007944.01.T01 |
| Chr2 | CDS | 23385356 | 23385625 | 23385356 | ID=MsG0280007944.01.T01:cds;Parent=MsG0280007944.01.T01 |
| Gene Sequence |
| Protein sequence |
