AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030555.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030555.01.T02 MTR_6g015780 94.915 59 3 0 1 59 48 106 2.98e-37 119
MsG0680030555.01.T02 MTR_6g015770 91.525 59 5 0 1 59 84 142 1.06e-35 117
MsG0680030555.01.T02 MTR_6g015715 92.857 28 2 0 1 28 48 75 2.57e-12 55.8
MsG0680030555.01.T01 MTR_6g015780 96.190 105 4 0 1 105 2 106 9.75e-72 209
MsG0680030555.01.T01 MTR_6g015770 94.253 87 5 0 19 105 56 142 2.32e-57 174
MsG0680030555.01.T01 MTR_6g015715 94.595 74 4 0 1 74 2 75 8.41e-45 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGCAGCAAGAATGCTTT+TGG 0.265450 6:-5254790 MsG0680030555.01.T01:CDS
AACAACATCATTGAAGATAA+AGG 0.308741 6:-5254877 MsG0680030555.01.T01:CDS
CTTGGACATGTTTCCATTTG+TGG 0.322045 6:-5254565 MsG0680030555.01.T01:three_prime_UTR
GTACGATAGGCTTGATCTAT+TGG 0.330021 6:+5254708 None:intergenic
TCAAGATATTGGAATAATCT+TGG 0.343890 6:-5254583 MsG0680030555.01.T01:three_prime_UTR
TTGTCTCATCAACAATGATT+TGG 0.352096 6:-5254838 MsG0680030555.01.T01:CDS
CACATATATATAACCACAAA+TGG 0.385939 6:+5254552 None:intergenic
TAAGTACCACACCTATTGTT+AGG 0.400080 6:+5254657 None:intergenic
ACAACATCATTGAAGATAAA+GGG 0.430981 6:-5254876 MsG0680030555.01.T01:CDS
CAAACACAAACTGGTAGAAC+TGG 0.449515 6:-5254759 MsG0680030555.01.T01:CDS
GATTTGAGTCAAACACAAAC+TGG 0.464256 6:-5254768 MsG0680030555.01.T01:CDS
ATGGAGGTGGACCTAACAAT+AGG 0.469104 6:-5254668 MsG0680030555.01.T01:CDS
AATATCTTGAATTAATGAAA+AGG 0.484100 6:+5254596 None:intergenic
GTAATTGGATCGATAAGATA+AGG 0.542896 6:+5254910 None:intergenic
AGAAGGCAAGCAAGTACGAT+AGG 0.551030 6:+5254695 None:intergenic
GTGAATAATTCCATGTGAGA+TGG 0.577986 6:-5254456 MsG0680030555.01.T01:three_prime_UTR
GCCAAAGAAGAAAATGAACA+AGG 0.614392 6:-5254949 None:intergenic
CCAAAGAAGAAAATGAACAA+GGG 0.665121 6:-5254948 None:intergenic
GGTAGAACTGGTAACAACAA+TGG 0.669601 6:-5254747 MsG0680030555.01.T01:CDS
GATAAAGGGTTGTACCTCAG+AGG 0.671194 6:-5254862 MsG0680030555.01.T01:CDS
GGTGGACCTAACAATAGGTG+TGG 0.694293 6:-5254663 MsG0680030555.01.T01:CDS
GTTGATGAGACAATCCTCTG+AGG 0.737752 6:+5254848 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCTTGAATTAATGAAA+AGG + Chr6:5254790-5254809 None:intergenic 15.0%
!!! TTTTCATTAATTCAAGATAT+TGG - Chr6:5254789-5254808 MsG0680030555.01.T01:CDS 15.0%
! AACAACATCATTGAAGATAA+AGG - Chr6:5254506-5254525 MsG0680030555.01.T01:three_prime_UTR 25.0%
! ACAACATCATTGAAGATAAA+GGG - Chr6:5254507-5254526 MsG0680030555.01.T01:three_prime_UTR 25.0%
! CACATATATATAACCACAAA+TGG + Chr6:5254834-5254853 None:intergenic 25.0%
! TCAAGATATTGGAATAATCT+TGG - Chr6:5254800-5254819 MsG0680030555.01.T01:CDS 25.0%
! TTCACAACAACAAAAATTCA+CGG + Chr6:5254912-5254931 None:intergenic 25.0%
GTAATTGGATCGATAAGATA+AGG + Chr6:5254476-5254495 None:intergenic 30.0%
TTGTCTCATCAACAATGATT+TGG - Chr6:5254545-5254564 MsG0680030555.01.T01:three_prime_UTR 30.0%
!! TTGTTTTGCTTAGGTGTAAT+TGG + Chr6:5254491-5254510 None:intergenic 30.0%
!!! TCAATGATGTTGTTTTGCTT+AGG + Chr6:5254500-5254519 None:intergenic 30.0%
GATTTGAGTCAAACACAAAC+TGG - Chr6:5254615-5254634 MsG0680030555.01.T01:three_prime_UTR 35.0%
GTGAATAATTCCATGTGAGA+TGG - Chr6:5254927-5254946 MsG0680030555.01.T01:CDS 35.0%
TAAGTACCACACCTATTGTT+AGG + Chr6:5254729-5254748 None:intergenic 35.0%
! ACTTGCTTGCCTTCTAAAAA+TGG - Chr6:5254696-5254715 MsG0680030555.01.T01:CDS 35.0%
CAAACACAAACTGGTAGAAC+TGG - Chr6:5254624-5254643 MsG0680030555.01.T01:exon 40.0%
CTTGGACATGTTTCCATTTG+TGG - Chr6:5254818-5254837 MsG0680030555.01.T01:CDS 40.0%
GGTAGAACTGGTAACAACAA+TGG - Chr6:5254636-5254655 MsG0680030555.01.T01:exon 40.0%
GTACGATAGGCTTGATCTAT+TGG + Chr6:5254678-5254697 None:intergenic 40.0%
! TCATGCAGCAAGAATGCTTT+TGG - Chr6:5254593-5254612 MsG0680030555.01.T01:three_prime_UTR 40.0%
! TGCTTGCCTTCTAAAAATGG+AGG - Chr6:5254699-5254718 MsG0680030555.01.T01:CDS 40.0%
! TTGCCTTCTAAAAATGGAGG+TGG - Chr6:5254702-5254721 MsG0680030555.01.T01:CDS 40.0%
AGAAGGCAAGCAAGTACGAT+AGG + Chr6:5254691-5254710 None:intergenic 45.0%
ATGGAGGTGGACCTAACAAT+AGG - Chr6:5254715-5254734 MsG0680030555.01.T01:CDS 45.0%
GATAAAGGGTTGTACCTCAG+AGG - Chr6:5254521-5254540 MsG0680030555.01.T01:three_prime_UTR 45.0%
! GTTGATGAGACAATCCTCTG+AGG + Chr6:5254538-5254557 None:intergenic 45.0%
!! GGTCCACCTCCATTTTTAGA+AGG + Chr6:5254708-5254727 None:intergenic 45.0%
GGTGGACCTAACAATAGGTG+TGG - Chr6:5254720-5254739 MsG0680030555.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 5254447 5254958 5254447 ID=MsG0680030555.01;Name=MsG0680030555.01
Chr6 mRNA 5254447 5254958 5254447 ID=MsG0680030555.01.T01;Parent=MsG0680030555.01;Name=MsG0680030555.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|194|105
Chr6 exon 5254447 5254958 5254447 ID=MsG0680030555.01.T01:exon:4377;Parent=MsG0680030555.01.T01
Chr6 CDS 5254641 5254958 5254641 ID=MsG0680030555.01.T01:cds;Parent=MsG0680030555.01.T01
Chr6 three_prime_UTR 5254447 5254640 5254447 ID=MsG0680030555.01.T01:three_prime_utr;Parent=MsG0680030555.01.T01
Chr6 mRNA 5254641 5254820 5254641 ID=MsG0680030555.01.T02;Parent=MsG0680030555.01;Name=MsG0680030555.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|59
Chr6 exon 5254641 5254820 5254641 ID=MsG0680030555.01.T02:exon:4378;Parent=MsG0680030555.01.T02
Chr6 CDS 5254641 5254820 5254641 ID=MsG0680030555.01.T02:cds;Parent=MsG0680030555.01.T02
Gene Sequence

>MsG0680030555.01.T02

ATGAGTTCTCATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAACAACAATGGTGCTGCTGTTAAGTGTCCAATAGATCAAGCCTATCGTACTTGCTTGCCTTCTAAAAATGGAGGTGGACCTAACAATAGGTGTGGTACTTACAATAGAGTTTGCTAA

>MsG0680030555.01.T01

ATGAACAAGGGTGTATCACTATTGTTCCTTATCTTATCGATCCAATTACACCTAAGCAAAACAACATCATTGAAGATAAAGGGTTGTACCTCAGAGGATTGTCTCATCAACAATGATTTGGAATCAGAAGAATTTTCTATGAGTTCTCATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAACAACAATGGTGCTGCTGTTAAGTGTCCAATAGATCAAGCCTATCGTACTTGCTTGCCTTCTAAAAATGGAGGTGGACCTAACAATAGGTGTGGTACTTACAATAGAGTTTGCTAA

Protein sequence

>MsG0680030555.01.T02

MSSHAARMLLDLSQTQTGRTGNNNGAAVKCPIDQAYRTCLPSKNGGGPNNRCGTYNRVC*

>MsG0680030555.01.T01

MNKGVSLLFLILSIQLHLSKTTSLKIKGCTSEDCLINNDLESEEFSMSSHAARMLLDLSQTQTGRTGNNNGAAVKCPIDQAYRTCLPSKNGGGPNNRCGTYNRVC*