Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004493.01.T01 | XP_013468987.1 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0 | 516 |
| MsG0180004493.01.T02 | XP_013468987.1 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0 | 516 |
| MsG0180004493.01.T03 | XP_013468987.1 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0 | 516 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004493.01.T01 | Q93VR3 | 88.302 | 265 | 21 | 2 | 1 | 263 | 121 | 377 | 2.82E-172 | 483 |
| MsG0180004493.01.T02 | Q93VR3 | 88.302 | 265 | 21 | 2 | 1 | 263 | 121 | 377 | 2.82E-172 | 483 |
| MsG0180004493.01.T03 | Q93VR3 | 88.302 | 265 | 21 | 2 | 1 | 263 | 121 | 377 | 2.82E-172 | 483 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004493.01.T01 | A0A072VLZ2 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0.0 | 516 |
| MsG0180004493.01.T02 | A0A072VLZ2 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0.0 | 516 |
| MsG0180004493.01.T03 | A0A072VLZ2 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0.0 | 516 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047899.01 | MsG0180004493.01 | 0.800887 | 1.167249e-48 | 5.497595e-46 |
| MsG0080048065.01 | MsG0180004493.01 | 0.847758 | 9.444324e-60 | 1.695784e-56 |
| MsG0080048152.01 | MsG0180004493.01 | 0.843837 | 1.096148e-58 | 1.736671e-55 |
| MsG0080048307.01 | MsG0180004493.01 | 0.871043 | 9.225216e-67 | 3.668155e-63 |
| MsG0080048463.01 | MsG0180004493.01 | 0.803996 | 2.670240e-49 | 1.361288e-46 |
| MsG0080048464.01 | MsG0180004493.01 | 0.825757 | 3.939277e-54 | 3.623370e-51 |
| MsG0080048660.01 | MsG0180004493.01 | 0.804438 | 2.159878e-49 | 1.113617e-46 |
| MsG0080048727.01 | MsG0180004493.01 | 0.844415 | 7.667374e-59 | 1.236914e-55 |
| MsG0080048769.01 | MsG0180004493.01 | 0.800250 | 1.573902e-48 | 7.293621e-46 |
| MsG0080048773.01 | MsG0180004493.01 | 0.860458 | 2.037101e-63 | 5.572387e-60 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004493.01.T01 | MTR_1g080950 | 94.382 | 267 | 5 | 2 | 1 | 265 | 121 | 379 | 0.0 | 516 |
| MsG0180004493.01.T01 | MTR_1g080950 | 94.382 | 267 | 5 | 2 | 1 | 265 | 157 | 415 | 0.0 | 513 |
| MsG0180004493.01.T01 | MTR_7g115080 | 87.925 | 265 | 21 | 3 | 1 | 263 | 122 | 377 | 9.98e-172 | 479 |
| MsG0180004493.01.T01 | MTR_7g115080 | 87.925 | 265 | 21 | 3 | 1 | 263 | 156 | 411 | 1.17e-171 | 480 |
| MsG0180004493.01.T01 | MTR_7g115080 | 87.925 | 265 | 21 | 3 | 1 | 263 | 135 | 390 | 2.82e-171 | 478 |
| MsG0180004493.01.T01 | MTR_4g120030 | 29.778 | 225 | 145 | 6 | 3 | 226 | 200 | 412 | 1.98e-21 | 93.2 |
| MsG0180004493.01.T01 | MTR_7g108490 | 28.755 | 233 | 151 | 8 | 2 | 228 | 97 | 320 | 9.86e-20 | 87.0 |
| MsG0180004493.01.T01 | MTR_7g108490 | 28.755 | 233 | 151 | 8 | 2 | 228 | 96 | 319 | 1.02e-19 | 87.0 |
| MsG0180004493.01.T01 | MTR_1g088480 | 27.586 | 232 | 155 | 6 | 3 | 233 | 207 | 426 | 1.24e-18 | 85.1 |
| MsG0180004493.01.T01 | MTR_4g063600 | 28.505 | 214 | 140 | 5 | 3 | 215 | 127 | 328 | 4.18e-17 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004493.01.T01 | AT5G28840 | 88.302 | 265 | 21 | 2 | 1 | 263 | 121 | 377 | 2.88e-173 | 483 |
| MsG0180004493.01.T01 | AT5G28840 | 88.302 | 265 | 21 | 2 | 1 | 263 | 121 | 377 | 2.88e-173 | 483 |
| MsG0180004493.01.T01 | AT1G73250 | 29.333 | 225 | 146 | 7 | 2 | 221 | 99 | 315 | 6.86e-21 | 90.5 |
| MsG0180004493.01.T01 | AT1G17890 | 28.511 | 235 | 153 | 8 | 2 | 230 | 94 | 319 | 1.81e-20 | 89.4 |
| MsG0180004493.01.T01 | AT1G17890 | 28.511 | 235 | 153 | 8 | 2 | 230 | 94 | 319 | 1.81e-20 | 89.4 |
| MsG0180004493.01.T01 | AT1G17890 | 28.511 | 235 | 153 | 8 | 2 | 230 | 102 | 327 | 1.99e-20 | 89.4 |
| MsG0180004493.01.T01 | AT3G62830 | 28.448 | 232 | 153 | 6 | 3 | 233 | 213 | 432 | 2.54e-20 | 90.1 |
| MsG0180004493.01.T01 | AT3G62830 | 28.448 | 232 | 153 | 6 | 3 | 233 | 213 | 432 | 2.54e-20 | 90.1 |
| MsG0180004493.01.T01 | AT2G47650 | 30.374 | 214 | 136 | 6 | 3 | 215 | 215 | 416 | 3.73e-20 | 89.7 |
| MsG0180004493.01.T01 | AT3G53520 | 28.814 | 236 | 154 | 7 | 1 | 234 | 212 | 435 | 9.36e-20 | 88.6 |
Find 67 sgRNAs with CRISPR-Local
Find 355 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCACTTGTCGGTGCTTG+GGG | 0.472833 | 1:+78843269 | MsG0180004493.01.T01:three_prime_UTR |
| AAAGCTTAAATTTCCATCAC+TGG | 0.449830 | 1:+78841112 | MsG0180004493.01.T01:five_prime_UTR |
| AAATTTCCATCACTGGTGCT+GGG | 0.427786 | 1:+78841119 | MsG0180004493.01.T01:five_prime_UTR |
| AACTGCTTAACGGTTACTAA+AGG | 0.478063 | 1:+78841283 | MsG0180004493.01.T01:five_prime_UTR |
| AAGCACTTGTCGGTGCTTGG+GGG | 0.603889 | 1:+78843270 | MsG0180004493.01.T01:three_prime_UTR |
| AATCTTGCCGCTGATATGGG+TGG | 0.591075 | 1:+78841322 | MsG0180004493.01.T01:five_prime_UTR |
| AATTCCATCTTGTTGATCTC+AGG | 0.363929 | 1:+78841251 | MsG0180004493.01.T01:five_prime_UTR |
| AATTTCCATCACTGGTGCTG+GGG | 0.500111 | 1:+78841120 | MsG0180004493.01.T01:five_prime_UTR |
| ACCCAAGCACCGGTCCAATT+AGG | 0.477394 | 1:+78843052 | MsG0180004493.01.T01:CDS |
| ACCCATTCCACCCATATCAG+CGG | 0.650854 | 1:-78841329 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGCATCAAACAACCAA+AGG | - | Chr1:78841680-78841699 | None:intergenic | 30.0% | |
| AAAATGGGTGAAAAAAGAAG+TGG | - | Chr1:78843603-78843622 | None:intergenic | 30.0% | |
| AAACATAGGATAACCTTCAT+AGG | - | Chr1:78844580-78844599 | None:intergenic | 30.0% | |
| AAAGCTTAAATTTCCATCAC+TGG | + | Chr1:78841112-78841131 | MsG0180004493.01.T01:five_prime_UTR | 30.0% | |
| AAATATTGGAAGTGATGAGA+TGG | + | Chr1:78842556-78842575 | MsG0180004493.01.T01:CDS | 30.0% | |
| AAATGGGTGAAAAAAGAAGT+GGG | - | Chr1:78843602-78843621 | None:intergenic | 30.0% | |
| AAATTCTGGTATTGTAGGAT+GGG | + | Chr1:78842933-78842952 | MsG0180004493.01.T01:intron | 30.0% | |
| AAGCACTACAACAAAGATTT+CGG | + | Chr1:78841808-78841827 | MsG0180004493.01.T01:CDS | 30.0% | |
| AATAAAAGAGAAACTTGGCT+GGG | + | Chr1:78842691-78842710 | MsG0180004493.01.T01:CDS | 30.0% | |
| AATCCATCTAAAAAGAAGGT+GGG | + | Chr1:78844991-78845010 | MsG0180004493.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 78840096 | 78845421 | 78840096 | ID=MsG0180004493.01;Name=MsG0180004493.01 |
| Chr1 | mRNA | 78840096 | 78845421 | 78840096 | ID=MsG0180004493.01.T01;Parent=MsG0180004493.01;Name=MsG0180004493.01.T01;_AED=0.26;_eAED=0.27;_QI=585|0.85|0.87|1|0.42|0.25|8|304|265 |
| Chr1 | five_prime_UTR | 78840096 | 78840318 | 78840096 | ID=MsG0180004493.01.T01:five_prime_utr;Parent=MsG0180004493.01.T01 |
| Chr1 | five_prime_UTR | 78841034 | 78841395 | 78841034 | ID=MsG0180004493.01.T01:five_prime_utr;Parent=MsG0180004493.01.T01 |
| Chr1 | CDS | 78841396 | 78841445 | 78841396 | ID=MsG0180004493.01.T01:cds;Parent=MsG0180004493.01.T01 |
| Chr1 | CDS | 78841566 | 78841662 | 78841566 | ID=MsG0180004493.01.T01:cds;Parent=MsG0180004493.01.T01 |
| Chr1 | CDS | 78841760 | 78841889 | 78841760 | ID=MsG0180004493.01.T01:cds;Parent=MsG0180004493.01.T01 |
| Chr1 | CDS | 78841990 | 78842140 | 78841990 | ID=MsG0180004493.01.T01:cds;Parent=MsG0180004493.01.T01 |
| Chr1 | CDS | 78842526 | 78842733 | 78842526 | ID=MsG0180004493.01.T01:cds;Parent=MsG0180004493.01.T01 |
| Chr1 | CDS | 78842950 | 78843111 | 78842950 | ID=MsG0180004493.01.T01:cds;Parent=MsG0180004493.01.T01 |
| Chr1 | three_prime_UTR | 78843112 | 78843393 | 78843112 | ID=MsG0180004493.01.T01:three_prime_utr;Parent=MsG0180004493.01.T01 |
| Chr1 | three_prime_UTR | 78845400 | 78845421 | 78845400 | ID=MsG0180004493.01.T01:three_prime_utr;Parent=MsG0180004493.01.T01 |
| Chr1 | mRNA | 78840096 | 78843491 | 78840096 | ID=MsG0180004493.01.T02;Parent=MsG0180004493.01;Name=MsG0180004493.01.T02;_AED=0.25;_eAED=0.26;_QI=585|0.83|0.85|1|0.5|0.42|7|380|265 |
| Chr1 | exon | 78842950 | 78843491 | 78842950 | ID=MsG0180004493.01.T02:exon:45469;Parent=MsG0180004493.01.T02 |
| Chr1 | five_prime_UTR | 78840096 | 78840318 | 78840096 | ID=MsG0180004493.01.T02:five_prime_utr;Parent=MsG0180004493.01.T02 |
| Chr1 | five_prime_UTR | 78841034 | 78841395 | 78841034 | ID=MsG0180004493.01.T02:five_prime_utr;Parent=MsG0180004493.01.T02 |
| Chr1 | CDS | 78841396 | 78841445 | 78841396 | ID=MsG0180004493.01.T02:cds;Parent=MsG0180004493.01.T02 |
| Chr1 | CDS | 78841566 | 78841662 | 78841566 | ID=MsG0180004493.01.T02:cds;Parent=MsG0180004493.01.T02 |
| Chr1 | CDS | 78841760 | 78841889 | 78841760 | ID=MsG0180004493.01.T02:cds;Parent=MsG0180004493.01.T02 |
| Chr1 | CDS | 78841990 | 78842140 | 78841990 | ID=MsG0180004493.01.T02:cds;Parent=MsG0180004493.01.T02 |
| Chr1 | CDS | 78842526 | 78842733 | 78842526 | ID=MsG0180004493.01.T02:cds;Parent=MsG0180004493.01.T02 |
| Chr1 | CDS | 78842950 | 78843111 | 78842950 | ID=MsG0180004493.01.T02:cds;Parent=MsG0180004493.01.T02 |
| Chr1 | three_prime_UTR | 78843112 | 78843491 | 78843112 | ID=MsG0180004493.01.T02:three_prime_utr;Parent=MsG0180004493.01.T02 |
| Chr1 | mRNA | 78840956 | 78845421 | 78840956 | ID=MsG0180004493.01.T03;Parent=MsG0180004493.01;Name=MsG0180004493.01.T03;_AED=0.28;_eAED=0.29;_QI=440|0.83|0.85|1|0.5|0.42|7|304|265 |
| Chr1 | exon | 78840956 | 78841445 | 78840956 | ID=MsG0180004493.01.T03:exon:45470;Parent=MsG0180004493.01.T03 |
| Chr1 | five_prime_UTR | 78840956 | 78841395 | 78840956 | ID=MsG0180004493.01.T03:five_prime_utr;Parent=MsG0180004493.01.T03 |
| Chr1 | CDS | 78841396 | 78841445 | 78841396 | ID=MsG0180004493.01.T03:cds;Parent=MsG0180004493.01.T03 |
| Chr1 | CDS | 78841566 | 78841662 | 78841566 | ID=MsG0180004493.01.T03:cds;Parent=MsG0180004493.01.T03 |
| Chr1 | CDS | 78841760 | 78841889 | 78841760 | ID=MsG0180004493.01.T03:cds;Parent=MsG0180004493.01.T03 |
| Chr1 | CDS | 78841990 | 78842140 | 78841990 | ID=MsG0180004493.01.T03:cds;Parent=MsG0180004493.01.T03 |
| Chr1 | CDS | 78842526 | 78842733 | 78842526 | ID=MsG0180004493.01.T03:cds;Parent=MsG0180004493.01.T03 |
| Chr1 | CDS | 78842950 | 78843111 | 78842950 | ID=MsG0180004493.01.T03:cds;Parent=MsG0180004493.01.T03 |
| Chr1 | three_prime_UTR | 78843112 | 78843393 | 78843112 | ID=MsG0180004493.01.T03:three_prime_utr;Parent=MsG0180004493.01.T03 |
| Chr1 | three_prime_UTR | 78845400 | 78845421 | 78845400 | ID=MsG0180004493.01.T03:three_prime_utr;Parent=MsG0180004493.01.T03 |
| Gene Sequence |
>MsG0180004493.01.T03 ATGATTAGTTTCAATATGATTGAAGCTGCTAGGATTAATGGAATTAAGAGGTTCTTCTATGCCTCAAGTGCTTGTATCTACCCTGAATTTAAACAGTTAGAAACTACTAATGTCAGCTTGAAGGAGTCTGATGCATGGCCTGCTGAGCCACAAGATGCGTATGGGCTAGAGAAGCTTGCAACAGAGGAGATTTGCAAGCACTACAACAAAGATTTCGGAATTGAGTGCCGCATTGGGAGGTTCCATAACATATATGGTCCTTTTGGAACATGGAAAGTTTGTTGCTTTGTCATACAGGTGGAAGGGAGAAGGCCTGCTGCTTTTTGTCGGAAAGCAATCACGTCTACAGACAAATTTGAGATGTGGGGAGATGGTTTGCAAACACGATCATTTACCTTCATTGATGAGTGTGTTGAAGGTGTACTTAGACTGACTAAATCCGATTTCCGCGAGCCGGTAAATATTGGAAGTGATGAGATGGTCAGCATGAATGAGATGGCTGAGATCGTTCTTGGTTTTGAGGACAAGAAGACTCCTATACATCACATTCCTGGCCCAGAGGGTGTTCGTGGTCGTAACTCAGACAATACACTAATAAAAGAGAAACTTGGCTGGGCTCCAACAATGAAGTTGAAGGATGGGCTGAGGATTACATATGTCTGGATTAAGGAACAACTTGAAAGGGAGAAGGCTCAAGGTCTTGATACATCAGGATACGGCTCATCAAAAGTGGTGCAAACCCAAGCACCGGTCCAATTAGGCTCACTTCGGGCAGCAGACGGCAAAGAGAGTAGTTAA >MsG0180004493.01.T01 ATGATTAGTTTCAATATGATTGAAGCTGCTAGGATTAATGGAATTAAGAGGTTCTTCTATGCCTCAAGTGCTTGTATCTACCCTGAATTTAAACAGTTAGAAACTACTAATGTCAGCTTGAAGGAGTCTGATGCATGGCCTGCTGAGCCACAAGATGCGTATGGGCTAGAGAAGCTTGCAACAGAGGAGATTTGCAAGCACTACAACAAAGATTTCGGAATTGAGTGCCGCATTGGGAGGTTCCATAACATATATGGTCCTTTTGGAACATGGAAAGTTTGTTGCTTTGTCATACAGGTGGAAGGGAGAAGGCCTGCTGCTTTTTGTCGGAAAGCAATCACGTCTACAGACAAATTTGAGATGTGGGGAGATGGTTTGCAAACACGATCATTTACCTTCATTGATGAGTGTGTTGAAGGTGTACTTAGACTGACTAAATCCGATTTCCGCGAGCCGGTAAATATTGGAAGTGATGAGATGGTCAGCATGAATGAGATGGCTGAGATCGTTCTTGGTTTTGAGGACAAGAAGACTCCTATACATCACATTCCTGGCCCAGAGGGTGTTCGTGGTCGTAACTCAGACAATACACTAATAAAAGAGAAACTTGGCTGGGCTCCAACAATGAAGTTGAAGGATGGGCTGAGGATTACATATGTCTGGATTAAGGAACAACTTGAAAGGGAGAAGGCTCAAGGTCTTGATACATCAGGATACGGCTCATCAAAAGTGGTGCAAACCCAAGCACCGGTCCAATTAGGCTCACTTCGGGCAGCAGACGGCAAAGAGAGTAGTTAA >MsG0180004493.01.T02 ATGATTAGTTTCAATATGATTGAAGCTGCTAGGATTAATGGAATTAAGAGGTTCTTCTATGCCTCAAGTGCTTGTATCTACCCTGAATTTAAACAGTTAGAAACTACTAATGTCAGCTTGAAGGAGTCTGATGCATGGCCTGCTGAGCCACAAGATGCGTATGGGCTAGAGAAGCTTGCAACAGAGGAGATTTGCAAGCACTACAACAAAGATTTCGGAATTGAGTGCCGCATTGGGAGGTTCCATAACATATATGGTCCTTTTGGAACATGGAAAGTTTGTTGCTTTGTCATACAGGTGGAAGGGAGAAGGCCTGCTGCTTTTTGTCGGAAAGCAATCACGTCTACAGACAAATTTGAGATGTGGGGAGATGGTTTGCAAACACGATCATTTACCTTCATTGATGAGTGTGTTGAAGGTGTACTTAGACTGACTAAATCCGATTTCCGCGAGCCGGTAAATATTGGAAGTGATGAGATGGTCAGCATGAATGAGATGGCTGAGATCGTTCTTGGTTTTGAGGACAAGAAGACTCCTATACATCACATTCCTGGCCCAGAGGGTGTTCGTGGTCGTAACTCAGACAATACACTAATAAAAGAGAAACTTGGCTGGGCTCCAACAATGAAGTTGAAGGATGGGCTGAGGATTACATATGTCTGGATTAAGGAACAACTTGAAAGGGAGAAGGCTCAAGGTCTTGATACATCAGGATACGGCTCATCAAAAGTGGTGCAAACCCAAGCACCGGTCCAATTAGGCTCACTTCGGGCAGCAGACGGCAAAGAGAGTAGTTAA |
| Protein sequence |
>MsG0180004493.01.T03 MISFNMIEAARINGIKRFFYASSACIYPEFKQLETTNVSLKESDAWPAEPQDAYGLEKLATEEICKHYNKDFGIECRIGRFHNIYGPFGTWKVCCFVIQVEGRRPAAFCRKAITSTDKFEMWGDGLQTRSFTFIDECVEGVLRLTKSDFREPVNIGSDEMVSMNEMAEIVLGFEDKKTPIHHIPGPEGVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYVWIKEQLEREKAQGLDTSGYGSSKVVQTQAPVQLGSLRAADGKESS* >MsG0180004493.01.T01 MISFNMIEAARINGIKRFFYASSACIYPEFKQLETTNVSLKESDAWPAEPQDAYGLEKLATEEICKHYNKDFGIECRIGRFHNIYGPFGTWKVCCFVIQVEGRRPAAFCRKAITSTDKFEMWGDGLQTRSFTFIDECVEGVLRLTKSDFREPVNIGSDEMVSMNEMAEIVLGFEDKKTPIHHIPGPEGVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYVWIKEQLEREKAQGLDTSGYGSSKVVQTQAPVQLGSLRAADGKESS* >MsG0180004493.01.T02 MISFNMIEAARINGIKRFFYASSACIYPEFKQLETTNVSLKESDAWPAEPQDAYGLEKLATEEICKHYNKDFGIECRIGRFHNIYGPFGTWKVCCFVIQVEGRRPAAFCRKAITSTDKFEMWGDGLQTRSFTFIDECVEGVLRLTKSDFREPVNIGSDEMVSMNEMAEIVLGFEDKKTPIHHIPGPEGVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYVWIKEQLEREKAQGLDTSGYGSSKVVQTQAPVQLGSLRAADGKESS* |
