Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007946.01.T01 | GAU26836.1 | 80 | 70 | 10 | 2 | 1 | 68 | 1 | 68 | 1.02E-28 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007946.01.T01 | A0A2Z6M4I6 | 80.000 | 70 | 10 | 2 | 1 | 68 | 1 | 68 | 4.86e-29 | 107 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007946.01 | MsG0280007947.01 | 0.946524 | 3.915013e-105 | 6.450638e-100 |
| MsG0280007946.01 | MsG0380014517.01 | 0.801441 | 8.989426e-49 | 4.293399e-46 |
| MsG0280007946.01 | MsG0380015847.01 | -0.803386 | 3.573464e-49 | 1.793307e-46 |
| MsG0280007946.01 | MsG0480021690.01 | 0.818920 | 1.523888e-52 | 1.156085e-49 |
| MsG0280007946.01 | MsG0580024435.01 | 0.839809 | 1.268729e-57 | 1.772217e-54 |
| MsG0280007946.01 | MsG0680034402.01 | 0.809490 | 1.844449e-50 | 1.084408e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007946.01.T01 | MTR_2g435780 | 96.078 | 51 | 2 | 0 | 1 | 51 | 1 | 51 | 7.48e-31 | 103 |
| MsG0280007946.01.T01 | MTR_2g435740 | 84.314 | 51 | 7 | 1 | 1 | 51 | 1 | 50 | 5.59e-24 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTAGTGCTTAAAATTTAA+AGG | 0.241643 | 2:+23495984 | None:intergenic |
| AGGGTCTGCCCCAACGATTT+TGG | 0.269496 | 2:+23496004 | None:intergenic |
| AGCATGCAAGATCGTGCTTC+TGG | 0.277502 | 2:-23496090 | MsG0280007946.01.T01:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | 2:-23496156 | MsG0280007946.01.T01:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.323679 | 2:+23496105 | None:intergenic |
| AAAGGGGACCCAAAATCGTT+GGG | 0.395758 | 2:-23496013 | MsG0280007946.01.T01:CDS |
| GCATGCAAGATCGTGCTTCT+GGG | 0.397696 | 2:-23496089 | MsG0280007946.01.T01:CDS |
| AGTAGTGCTTAAAATTTAAA+GGG | 0.405868 | 2:+23495985 | None:intergenic |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | 2:-23496165 | MsG0280007946.01.T01:CDS |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | 2:+23496157 | None:intergenic |
| GCTTCTGGGGTTAGCAAGGA+TGG | 0.500998 | 2:-23496075 | MsG0280007946.01.T01:CDS |
| TCGAATGTAAGTTACCCAAA+GGG | 0.506137 | 2:-23496030 | MsG0280007946.01.T01:CDS |
| CAAAGGGGACCCAAAATCGT+TGG | 0.523644 | 2:-23496014 | MsG0280007946.01.T01:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | 2:-23496155 | MsG0280007946.01.T01:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | 2:-23496173 | MsG0280007946.01.T01:CDS |
| CATGCAAGATCGTGCTTCTG+GGG | 0.577242 | 2:-23496088 | MsG0280007946.01.T01:CDS |
| TCGTGCTTCTGGGGTTAGCA+AGG | 0.589442 | 2:-23496079 | MsG0280007946.01.T01:CDS |
| ATCGAATGTAAGTTACCCAA+AGG | 0.592367 | 2:-23496031 | MsG0280007946.01.T01:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | 2:+23496168 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | 2:+23496171 | None:intergenic |
| AAGGGGACCCAAAATCGTTG+GGG | 0.676227 | 2:-23496012 | MsG0280007946.01.T01:CDS |
| CGAATGTAAGTTACCCAAAG+GGG | 0.682969 | 2:-23496029 | MsG0280007946.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCTTTAATAGACAAGTTTTA+TGG | - | Chr2:23496041-23496060 | MsG0280007946.01.T01:CDS | 25.0% |
| ATCGAATGTAAGTTACCCAA+AGG | - | Chr2:23496139-23496158 | MsG0280007946.01.T01:CDS | 35.0% | |
| TCGAATGTAAGTTACCCAAA+GGG | - | Chr2:23496140-23496159 | MsG0280007946.01.T01:CDS | 35.0% | |
| ! | GTTTTATGGTCCAACAAGAA+GGG | - | Chr2:23496055-23496074 | MsG0280007946.01.T01:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | - | Chr2:23496054-23496073 | MsG0280007946.01.T01:CDS | 35.0% |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | - | Chr2:23496015-23496034 | MsG0280007946.01.T01:CDS | 35.0% |
| ATCTTCCTTAGGTCTGATGA+AGG | + | Chr2:23496005-23496024 | None:intergenic | 40.0% | |
| CGAATGTAAGTTACCCAAAG+GGG | - | Chr2:23496141-23496160 | MsG0280007946.01.T01:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | + | Chr2:23496016-23496035 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | - | Chr2:23496005-23496024 | MsG0280007946.01.T01:CDS | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | - | Chr2:23496014-23496033 | MsG0280007946.01.T01:CDS | 40.0% |
| AAAGGGGACCCAAAATCGTT+GGG | - | Chr2:23496157-23496176 | MsG0280007946.01.T01:CDS | 45.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | + | Chr2:23496002-23496021 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTT+GGG | + | Chr2:23496157-23496176 | None:intergenic | 45.0% |
| AAGGGGACCCAAAATCGTTG+GGG | - | Chr2:23496158-23496177 | MsG0280007946.01.T01:CDS | 50.0% | |
| AGCATGCAAGATCGTGCTTC+TGG | - | Chr2:23496080-23496099 | MsG0280007946.01.T01:CDS | 50.0% | |
| CAAAGGGGACCCAAAATCGT+TGG | - | Chr2:23496156-23496175 | MsG0280007946.01.T01:CDS | 50.0% | |
| CACCTCCTTCATCAGACCTA+AGG | - | Chr2:23495997-23496016 | MsG0280007946.01.T01:CDS | 50.0% | |
| CATGCAAGATCGTGCTTCTG+GGG | - | Chr2:23496082-23496101 | MsG0280007946.01.T01:CDS | 50.0% | |
| GCATGCAAGATCGTGCTTCT+GGG | - | Chr2:23496081-23496100 | MsG0280007946.01.T01:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | + | Chr2:23496068-23496087 | None:intergenic | 50.0% | |
| !! | CAACGATTTTGGGTCCCCTT+TGG | + | Chr2:23496158-23496177 | None:intergenic | 50.0% |
| ! | AGGGTCTGCCCCAACGATTT+TGG | + | Chr2:23496169-23496188 | None:intergenic | 55.0% |
| ! | GGGTCTGCCCCAACGATTTT+GGG | + | Chr2:23496168-23496187 | None:intergenic | 55.0% |
| !! | GCTTCTGGGGTTAGCAAGGA+TGG | - | Chr2:23496095-23496114 | MsG0280007946.01.T01:CDS | 55.0% |
| !! | TCGTGCTTCTGGGGTTAGCA+AGG | - | Chr2:23496091-23496110 | MsG0280007946.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 23495993 | 23496199 | 23495993 | ID=MsG0280007946.01;Name=MsG0280007946.01 |
| Chr2 | mRNA | 23495993 | 23496199 | 23495993 | ID=MsG0280007946.01.T01;Parent=MsG0280007946.01;Name=MsG0280007946.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|68 |
| Chr2 | exon | 23495993 | 23496199 | 23495993 | ID=MsG0280007946.01.T01:exon:18504;Parent=MsG0280007946.01.T01 |
| Chr2 | CDS | 23495993 | 23496199 | 23495993 | ID=MsG0280007946.01.T01:cds;Parent=MsG0280007946.01.T01 |
| Gene Sequence |
| Protein sequence |
