Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007947.01.T01 | GAU26836.1 | 80 | 70 | 10 | 2 | 1 | 68 | 1 | 68 | 1.02E-28 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007947.01.T01 | A0A2Z6M4I6 | 80.000 | 70 | 10 | 2 | 1 | 68 | 1 | 68 | 4.86e-29 | 107 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048748.01 | MsG0280007947.01 | 0.828551 | 8.441512e-55 | 8.418361e-52 |
| MsG0080048938.01 | MsG0280007947.01 | 0.838852 | 2.248522e-57 | 3.049864e-54 |
| MsG0180000255.01 | MsG0280007947.01 | 0.816549 | 5.222652e-52 | 3.711799e-49 |
| MsG0180000784.01 | MsG0280007947.01 | 0.814172 | 1.763830e-51 | 1.174979e-48 |
| MsG0180000814.01 | MsG0280007947.01 | 0.809670 | 1.687337e-50 | 9.968492e-48 |
| MsG0180001100.01 | MsG0280007947.01 | 0.806407 | 8.351504e-50 | 4.529254e-47 |
| MsG0180004541.01 | MsG0280007947.01 | 0.801299 | 9.612809e-49 | 4.574724e-46 |
| MsG0180005339.01 | MsG0280007947.01 | 0.821354 | 4.222486e-53 | 3.427176e-50 |
| MsG0280007946.01 | MsG0280007947.01 | 0.946524 | 3.915013e-105 | 6.450638e-100 |
| MsG0280007947.01 | MsG0280008072.01 | 0.813440 | 2.556452e-51 | 1.669635e-48 |
| MsG0280007947.01 | MsG0280008098.01 | 0.842697 | 2.206727e-58 | 3.373018e-55 |
| MsG0280007947.01 | MsG0280009507.01 | 0.804174 | 2.451458e-49 | 1.255431e-46 |
| MsG0280007947.01 | MsG0280009699.01 | 0.820496 | 6.651068e-53 | 5.271079e-50 |
| MsG0280007947.01 | MsG0380014517.01 | 0.811557 | 6.595996e-51 | 4.095815e-48 |
| MsG0280007947.01 | MsG0380015847.01 | -0.856917 | 2.326634e-62 | 5.642033e-59 |
| MsG0280007947.01 | MsG0380015885.01 | 0.803948 | 2.732210e-49 | 1.391193e-46 |
| MsG0280007947.01 | MsG0380017338.01 | 0.808485 | 3.026583e-50 | 1.732683e-47 |
| MsG0280007947.01 | MsG0480018426.01 | 0.814081 | 1.847820e-51 | 1.227905e-48 |
| MsG0280007947.01 | MsG0480018848.01 | 0.806439 | 8.221158e-50 | 4.462365e-47 |
| MsG0280007947.01 | MsG0480021690.01 | 0.839755 | 1.310503e-57 | 1.827355e-54 |
| MsG0280007947.01 | MsG0480022239.01 | 0.801515 | 8.683918e-49 | 4.155182e-46 |
| MsG0280007947.01 | MsG0480022537.01 | 0.804695 | 1.909474e-49 | 9.910953e-47 |
| MsG0280007947.01 | MsG0480022918.01 | 0.806637 | 7.466924e-50 | 4.073287e-47 |
| MsG0280007947.01 | MsG0580024435.01 | 0.864640 | 1.051274e-64 | 3.324891e-61 |
| MsG0280007947.01 | MsG0580024775.01 | 0.809105 | 2.230229e-50 | 1.297875e-47 |
| MsG0280007947.01 | MsG0580025280.01 | 0.803385 | 3.575611e-49 | 1.794329e-46 |
| MsG0280007947.01 | MsG0580025670.01 | -0.816294 | 5.955536e-52 | 4.203672e-49 |
| MsG0280007947.01 | MsG0580025827.01 | 0.812704 | 3.707616e-51 | 2.373957e-48 |
| MsG0280007947.01 | MsG0580025906.01 | 0.805275 | 1.444185e-49 | 7.608290e-47 |
| MsG0280007947.01 | MsG0580029912.01 | 0.808436 | 3.099839e-50 | 1.772406e-47 |
| MsG0280007947.01 | MsG0680034402.01 | 0.805257 | 1.457029e-49 | 7.672300e-47 |
| MsG0280007947.01 | MsG0680035707.01 | 0.809430 | 1.900086e-50 | 1.115368e-47 |
| MsG0280007947.01 | MsG0780039336.01 | 0.807806 | 4.221502e-50 | 2.373953e-47 |
| MsG0280007947.01 | MsG0780040177.01 | 0.812833 | 3.473830e-51 | 2.231677e-48 |
| MsG0280007947.01 | MsG0780040278.01 | 0.813296 | 2.750332e-51 | 1.789126e-48 |
| MsG0280007947.01 | MsG0780041301.01 | 0.808179 | 3.517022e-50 | 1.997401e-47 |
| MsG0280007947.01 | MsG0780041747.01 | 0.809735 | 1.633626e-50 | 9.667792e-48 |
| MsG0280007947.01 | MsG0880042086.01 | 0.804361 | 2.241854e-49 | 1.153616e-46 |
| MsG0280007947.01 | MsG0880042416.01 | 0.826123 | 3.224109e-54 | 2.996595e-51 |
| MsG0280007947.01 | MsG0880042417.01 | 0.816743 | 4.725904e-52 | 3.376684e-49 |
| MsG0280007947.01 | MsG0880043829.01 | 0.821278 | 4.396551e-53 | 3.560775e-50 |
| MsG0280007947.01 | MsG0880045285.01 | 0.824603 | 7.379338e-54 | 6.568420e-51 |
| MsG0280007947.01 | MsG0880045783.01 | 0.812131 | 4.946341e-51 | 3.119040e-48 |
| MsG0280007947.01 | MsG0880045992.01 | 0.802739 | 4.863921e-49 | 2.400968e-46 |
| MsG0280007947.01 | MsG0880046334.01 | -0.827537 | 1.481509e-54 | 1.434291e-51 |
| MsG0280007947.01 | MsG0880047045.01 | 0.840199 | 1.004088e-57 | 1.419781e-54 |
| MsG0280007947.01 | MsG0880047046.01 | 0.815650 | 8.290591e-52 | 5.749676e-49 |
| MsG0280007947.01 | MsG0880047152.01 | 0.815582 | 8.587574e-52 | 5.944094e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007947.01.T01 | MTR_2g435780 | 96.078 | 51 | 2 | 0 | 1 | 51 | 1 | 51 | 7.48e-31 | 103 |
| MsG0280007947.01.T01 | MTR_2g435740 | 84.314 | 51 | 7 | 1 | 1 | 51 | 1 | 50 | 5.59e-24 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTAGTGCTTAAAATTTAA+AGG | 0.241643 | 2:+23508774 | None:intergenic |
| AGGGTCTACCCCAACGATTT+TGG | 0.250377 | 2:+23508794 | None:intergenic |
| AGCATGCAAGATCGTGCTTC+TGG | 0.277502 | 2:-23508880 | MsG0280007947.01.T01:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | 2:-23508946 | MsG0280007947.01.T01:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.323679 | 2:+23508895 | None:intergenic |
| GCATGCAAGATCGTGCTTCT+GGG | 0.397696 | 2:-23508879 | MsG0280007947.01.T01:CDS |
| AGTAGTGCTTAAAATTTAAA+GGG | 0.405868 | 2:+23508775 | None:intergenic |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | 2:-23508955 | MsG0280007947.01.T01:CDS |
| AAAGGGGACCCAAAATCGTT+GGG | 0.417735 | 2:-23508803 | MsG0280007947.01.T01:CDS |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | 2:+23508947 | None:intergenic |
| GCTTCTGGGGTTAGCAAGGA+TGG | 0.500998 | 2:-23508865 | MsG0280007947.01.T01:CDS |
| TCGAATGTAAGTTACCCAAA+GGG | 0.506137 | 2:-23508820 | MsG0280007947.01.T01:CDS |
| CAAAGGGGACCCAAAATCGT+TGG | 0.523644 | 2:-23508804 | MsG0280007947.01.T01:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | 2:-23508945 | MsG0280007947.01.T01:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | 2:-23508963 | MsG0280007947.01.T01:CDS |
| CATGCAAGATCGTGCTTCTG+GGG | 0.577242 | 2:-23508878 | MsG0280007947.01.T01:CDS |
| TCGTGCTTCTGGGGTTAGCA+AGG | 0.589442 | 2:-23508869 | MsG0280007947.01.T01:CDS |
| ATCGAATGTAAGTTACCCAA+AGG | 0.592367 | 2:-23508821 | MsG0280007947.01.T01:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | 2:+23508958 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | 2:+23508961 | None:intergenic |
| AAGGGGACCCAAAATCGTTG+GGG | 0.681552 | 2:-23508802 | MsG0280007947.01.T01:CDS |
| CGAATGTAAGTTACCCAAAG+GGG | 0.682969 | 2:-23508819 | MsG0280007947.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCTTTAATAGACAAGTTTTA+TGG | - | Chr2:23508831-23508850 | MsG0280007947.01.T01:CDS | 25.0% |
| ATCGAATGTAAGTTACCCAA+AGG | - | Chr2:23508929-23508948 | MsG0280007947.01.T01:CDS | 35.0% | |
| TCGAATGTAAGTTACCCAAA+GGG | - | Chr2:23508930-23508949 | MsG0280007947.01.T01:CDS | 35.0% | |
| ! | GTTTTATGGTCCAACAAGAA+GGG | - | Chr2:23508845-23508864 | MsG0280007947.01.T01:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | - | Chr2:23508844-23508863 | MsG0280007947.01.T01:CDS | 35.0% |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | - | Chr2:23508805-23508824 | MsG0280007947.01.T01:CDS | 35.0% |
| ATCTTCCTTAGGTCTGATGA+AGG | + | Chr2:23508795-23508814 | None:intergenic | 40.0% | |
| CGAATGTAAGTTACCCAAAG+GGG | - | Chr2:23508931-23508950 | MsG0280007947.01.T01:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | + | Chr2:23508806-23508825 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | - | Chr2:23508795-23508814 | MsG0280007947.01.T01:CDS | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | - | Chr2:23508804-23508823 | MsG0280007947.01.T01:CDS | 40.0% |
| AAAGGGGACCCAAAATCGTT+GGG | - | Chr2:23508947-23508966 | MsG0280007947.01.T01:CDS | 45.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | + | Chr2:23508792-23508811 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTT+GGG | + | Chr2:23508947-23508966 | None:intergenic | 45.0% |
| AAGGGGACCCAAAATCGTTG+GGG | - | Chr2:23508948-23508967 | MsG0280007947.01.T01:CDS | 50.0% | |
| AGCATGCAAGATCGTGCTTC+TGG | - | Chr2:23508870-23508889 | MsG0280007947.01.T01:CDS | 50.0% | |
| CAAAGGGGACCCAAAATCGT+TGG | - | Chr2:23508946-23508965 | MsG0280007947.01.T01:CDS | 50.0% | |
| CACCTCCTTCATCAGACCTA+AGG | - | Chr2:23508787-23508806 | MsG0280007947.01.T01:CDS | 50.0% | |
| CATGCAAGATCGTGCTTCTG+GGG | - | Chr2:23508872-23508891 | MsG0280007947.01.T01:CDS | 50.0% | |
| GCATGCAAGATCGTGCTTCT+GGG | - | Chr2:23508871-23508890 | MsG0280007947.01.T01:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | + | Chr2:23508858-23508877 | None:intergenic | 50.0% | |
| ! | AGGGTCTACCCCAACGATTT+TGG | + | Chr2:23508959-23508978 | None:intergenic | 50.0% |
| ! | GGGTCTACCCCAACGATTTT+GGG | + | Chr2:23508958-23508977 | None:intergenic | 50.0% |
| !! | CAACGATTTTGGGTCCCCTT+TGG | + | Chr2:23508948-23508967 | None:intergenic | 50.0% |
| !! | GCTTCTGGGGTTAGCAAGGA+TGG | - | Chr2:23508885-23508904 | MsG0280007947.01.T01:CDS | 55.0% |
| !! | TCGTGCTTCTGGGGTTAGCA+AGG | - | Chr2:23508881-23508900 | MsG0280007947.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 23508783 | 23508989 | 23508783 | ID=MsG0280007947.01;Name=MsG0280007947.01 |
| Chr2 | mRNA | 23508783 | 23508989 | 23508783 | ID=MsG0280007947.01.T01;Parent=MsG0280007947.01;Name=MsG0280007947.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|68 |
| Chr2 | exon | 23508783 | 23508989 | 23508783 | ID=MsG0280007947.01.T01:exon:4540;Parent=MsG0280007947.01.T01 |
| Chr2 | CDS | 23508783 | 23508989 | 23508783 | ID=MsG0280007947.01.T01:cds;Parent=MsG0280007947.01.T01 |
| Gene Sequence |
| Protein sequence |
