AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008108.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008108.01.T01 MTR_2g035790 94.057 488 22 2 4 484 1 488 0.0 880
MsG0280008108.01.T01 MTR_4g094732 66.804 485 152 6 4 484 1 480 0.0 624
MsG0280008108.01.T01 MTR_2g035760 52.657 414 176 8 65 472 82 481 1.08e-140 414
MsG0280008108.01.T01 MTR_6g015785 52.998 417 173 7 72 484 93 490 1.34e-134 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008108.01.T01 AT4G20040 58.635 469 180 7 24 484 21 483 0.0 541
MsG0280008108.01.T01 AT4G20050 55.059 425 167 7 65 484 74 479 2.49e-151 440
MsG0280008108.01.T01 AT4G20050 55.059 425 167 7 65 484 74 479 2.49e-151 440
MsG0280008108.01.T01 AT4G20050 55.059 425 167 7 65 484 74 479 2.49e-151 440
MsG0280008108.01.T01 AT4G20050 55.059 425 167 7 65 484 74 479 2.49e-151 440
MsG0280008108.01.T01 AT4G20050 55.059 425 167 7 65 484 99 504 2.52e-151 441
MsG0280008108.01.T01 AT4G20050 54.750 400 157 7 90 484 1 381 1.75e-139 406
MsG0280008108.01.T01 AT4G20050 54.750 400 157 7 90 484 1 381 1.75e-139 406

Find 88 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTTGATATTGATAATTT+AGG 0.107857 2:+25570328 MsG0280008108.01.T01:CDS
ACAATTGGAACATTATTATT+AGG 0.129299 2:-25573000 None:intergenic
GGATCTGCACCATACTTAAT+TGG 0.153680 2:-25570256 None:intergenic
TCAATTAAAGGGAAAGTTTA+TGG 0.171584 2:+25572943 MsG0280008108.01.T01:CDS
AGCATTTCTCTTATCACTTT+TGG 0.183808 2:+25569793 MsG0280008108.01.T01:CDS
CACCATCCTCACACACAAAA+AGG 0.217441 2:+25569930 MsG0280008108.01.T01:CDS
TGTATGCTGTTGTTGCATTT+TGG 0.236487 2:-25572372 None:intergenic
CATTTGATATTGATAATTTA+GGG 0.251344 2:+25570329 MsG0280008108.01.T01:CDS
GTTCCAATTGTAAAATTAGA+TGG 0.268649 2:+25573012 MsG0280008108.01.T01:CDS
CAATTGGAACATTATTATTA+GGG 0.285274 2:-25572999 None:intergenic
TATAACAAAGCAACAGGTTT+TGG 0.286379 2:+25572769 MsG0280008108.01.T01:CDS
TGAAAATGACTAATCCTATT+AGG 0.294977 2:-25573174 None:intergenic
ATTGTTAATAATATGTTTAG+TGG 0.301676 2:+25572973 MsG0280008108.01.T01:CDS
GTTCAAGTTCATGTTACTAA+TGG 0.312426 2:+25572886 MsG0280008108.01.T01:CDS
CAGCCATTGGAATTGCTGTT+AGG 0.315911 2:+25572713 MsG0280008108.01.T01:CDS
AAGAAAACTACTTGATATAA+AGG 0.336779 2:-25572581 None:intergenic
AAATGTATTGGAAGCTCTTA+AGG 0.341331 2:-25572299 None:intergenic
TGGATGGTAATGGCACAAAA+TGG 0.367127 2:+25573121 MsG0280008108.01.T01:CDS
TAATTCCTTCTGTGTTGAAA+TGG 0.369391 2:-25572537 None:intergenic
CCTCCAAGGTCTCTAATTCC+TGG 0.397675 2:-25570364 None:intergenic
TACTTGATATAAAGGTTTCA+TGG 0.400711 2:-25572573 None:intergenic
CATTGTTATAACAAAGCAAC+AGG 0.404226 2:+25572763 MsG0280008108.01.T01:CDS
TTGATCAAGTGGTGATTGAT+AGG 0.431617 2:+25573049 MsG0280008108.01.T01:CDS
AGCTTCTACAGCTTTCATAA+TGG 0.438110 2:-25570305 None:intergenic
AAGTATGGTGCAGATCCAAC+AGG 0.439437 2:+25570262 MsG0280008108.01.T01:CDS
GATCTGCACCATACTTAATT+GGG 0.441924 2:-25570255 None:intergenic
ATCACATTTCCTTCATCTGT+TGG 0.444373 2:+25570433 MsG0280008108.01.T01:CDS
TTAGTAACATGAACTTGAAC+AGG 0.445078 2:-25572883 None:intergenic
GAATTACTTCAACATACAAT+AGG 0.445561 2:+25572400 MsG0280008108.01.T01:CDS
ACTGAATGAGCAACAAACTT+TGG 0.451213 2:-25573222 None:intergenic
TCTATCAGTTGGAAATGTAT+TGG 0.453459 2:-25572311 None:intergenic
TTCCAATTGTAAAATTAGAT+GGG 0.461356 2:+25573013 MsG0280008108.01.T01:CDS
AGCCATTGGAATTGCTGTTA+GGG 0.462522 2:+25572714 MsG0280008108.01.T01:CDS
AAGGAAACAACACCATGAAC+TGG 0.487041 2:-25573288 None:intergenic
TTCATCTGTTGGCAATCTTG+TGG 0.488576 2:+25570444 MsG0280008108.01.T01:CDS
TTACCAGGAATTAGAGACCT+TGG 0.494991 2:+25570361 MsG0280008108.01.T01:CDS
GATTCGAGCTACCGAGGTGG+AGG 0.505643 2:+25572463 MsG0280008108.01.T01:CDS
TTCCTCCATTTCAACACAGA+AGG 0.508555 2:+25572532 MsG0280008108.01.T01:CDS
AAGAATTGATAATTGTTACA+TGG 0.510868 2:+25572834 MsG0280008108.01.T01:CDS
TGTTGAACAATGAGTTGGTA+TGG 0.511720 2:+25573314 MsG0280008108.01.T01:exon
AACTCATTGTTCAACAAAGA+AGG 0.513850 2:-25573307 None:intergenic
TTAGGGCTTGAATTATTACC+AGG 0.514044 2:+25570346 MsG0280008108.01.T01:CDS
TTCTTTGTTGAACAATGAGT+TGG 0.516326 2:+25573309 MsG0280008108.01.T01:exon
ACAAGATTGCCAACAGATGA+AGG 0.517369 2:-25570442 None:intergenic
GTGGTGTACCCAATTAAGTA+TGG 0.526549 2:+25570247 MsG0280008108.01.T01:CDS
CTTGGACAACACTCAACAGT+CGG 0.527472 2:+25572601 MsG0280008108.01.T01:CDS
TCAACAAGGTATCTATCAGT+TGG 0.531153 2:-25572322 None:intergenic
GAAAGTGAGAAACCAGTTCA+TGG 0.531426 2:+25573276 MsG0280008108.01.T01:CDS
ATGAAATGTTTGAAGGTGAA+AGG 0.538395 2:+25572271 MsG0280008108.01.T01:intron
GATGGTGATGGAGAAGGAGA+GGG 0.541177 2:-25569914 None:intergenic
ATTGATAGGAACAATGTGAT+TGG 0.546202 2:+25573063 MsG0280008108.01.T01:CDS
GAAGGAATTAAAGTTAAAAG+TGG 0.548093 2:+25572550 MsG0280008108.01.T01:CDS
TGGTAATGGCACAAAATGGG+TGG 0.549729 2:+25573125 MsG0280008108.01.T01:CDS
TTCCTTCTGTGTTGAAATGG+AGG 0.550409 2:-25572534 None:intergenic
GGATGGTAATGGCACAAAAT+GGG 0.551911 2:+25573122 MsG0280008108.01.T01:CDS
GGTGATGGAGAAGGAGAGGG+TGG 0.556663 2:-25569911 None:intergenic
TCAACAGTCGGCGGAGACAA+AGG 0.561802 2:+25572613 MsG0280008108.01.T01:CDS
GGATTATAATTCTATAGTCA+TGG 0.563335 2:+25572855 MsG0280008108.01.T01:CDS
GGATGGTGATGGAGAAGGAG+AGG 0.566266 2:-25569915 None:intergenic
TCCAATTGTAAAATTAGATG+GGG 0.567825 2:+25573014 MsG0280008108.01.T01:CDS
ATTTAGTAACATTGATCAAG+TGG 0.575859 2:+25573038 MsG0280008108.01.T01:CDS
TGTGAGGATGGTGATGGAGA+AGG 0.580376 2:-25569920 None:intergenic
AAGTGATAAGAGAAATGCTA+AGG 0.585584 2:-25569789 None:intergenic
CCAGGAATTAGAGACCTTGG+AGG 0.587401 2:+25570364 MsG0280008108.01.T01:CDS
GGACAAGCTAACATAATCAC+AGG 0.590351 2:+25572736 MsG0280008108.01.T01:CDS
ACAGATGAAGGAAATGTGAT+GGG 0.598207 2:-25570430 None:intergenic
CTCTTCGATTCGAGCTACCG+AGG 0.599335 2:+25572457 MsG0280008108.01.T01:CDS
CCAATTGTAAAATTAGATGG+GGG 0.605323 2:+25573015 MsG0280008108.01.T01:CDS
AACAGATGAAGGAAATGTGA+TGG 0.606062 2:-25570431 None:intergenic
TTTGTGTGTGAGGATGGTGA+TGG 0.607217 2:-25569926 None:intergenic
TAGCAATGACAATGCTATCA+CGG 0.608730 2:+25572672 MsG0280008108.01.T01:CDS
AATCAATAATAACACCTCCA+AGG 0.611738 2:-25570378 None:intergenic
TACGTGTTCATTGAAGCAAG+TGG 0.612140 2:-25569828 None:intergenic
AGGAGAGGGTGGTTGTGACA+AGG 0.615820 2:-25569900 None:intergenic
TCCCCTAACAGCAATTCCAA+TGG 0.616503 2:-25572716 None:intergenic
AAGTTGAATGTGGATGGTAA+TGG 0.619073 2:+25573111 MsG0280008108.01.T01:CDS
TTCGATTCGAGCTACCGAGG+TGG 0.620470 2:+25572460 MsG0280008108.01.T01:CDS
GCCATTGGAATTGCTGTTAG+GGG 0.628491 2:+25572715 MsG0280008108.01.T01:CDS
TTGTTGCACACAACTCAACA+AGG 0.640430 2:-25572336 None:intergenic
GGTAATGGCACAAAATGGGT+GGG 0.641219 2:+25573126 MsG0280008108.01.T01:CDS
AACGGTTGGAAAGTTGAATG+TGG 0.643847 2:+25573101 MsG0280008108.01.T01:CDS
AGTATGGTGCAGATCCAACA+GGG 0.649273 2:+25570263 MsG0280008108.01.T01:CDS
GTTGGAAAGTTGAATGTGGA+TGG 0.650817 2:+25573105 MsG0280008108.01.T01:CDS
AAATGTTTGAAGGTGAAAGG+AGG 0.651730 2:+25572274 MsG0280008108.01.T01:intron
CTGTAATCAGAGTGGAAGAG+TGG 0.673872 2:+25570228 MsG0280008108.01.T01:intron
GGACAACACTCAACAGTCGG+CGG 0.701448 2:+25572604 MsG0280008108.01.T01:CDS
CGATGAGTATTCCTCCACCT+CGG 0.732507 2:-25572474 None:intergenic
GTATGGTGCAGATCCAACAG+GGG 0.763386 2:+25570264 MsG0280008108.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTGAAAAATATTTATA+AGG - Chr2:25571001-25571020 None:intergenic 10.0%
!! AAAGTGAAAAATATTTATAA+GGG - Chr2:25571000-25571019 None:intergenic 10.0%
!! AAATTAATCAATAGTTAATA+AGG + Chr2:25571452-25571471 MsG0280008108.01.T01:intron 10.0%
!! AATAGTAAAAAGTAAAAAAA+TGG + Chr2:25571804-25571823 MsG0280008108.01.T01:intron 10.0%
!! TTAAAGAAAAAATAATACTT+TGG + Chr2:25570793-25570812 MsG0280008108.01.T01:intron 10.0%
!!! AATTTTATTTTACCTAAATT+AGG + Chr2:25570631-25570650 MsG0280008108.01.T01:intron 10.0%
!! AACTTTATAAAAATGATAGT+TGG + Chr2:25570594-25570613 MsG0280008108.01.T01:intron 15.0%
!! ATTGTTAATAATATGTTTAG+TGG + Chr2:25572973-25572992 MsG0280008108.01.T01:CDS 15.0%
!! GTAACATAATATTAATTGAA+TGG + Chr2:25570823-25570842 MsG0280008108.01.T01:intron 15.0%
!!! CATTTGATATTGATAATTTA+GGG + Chr2:25570329-25570348 MsG0280008108.01.T01:CDS 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG + Chr2:25570897-25570916 MsG0280008108.01.T01:intron 15.0%
!!! TATTCATTTTATACATTAGA+GGG + Chr2:25573198-25573217 MsG0280008108.01.T01:CDS 15.0%
!!! TCATACATTTTTTTAGATTT+TGG + Chr2:25570054-25570073 MsG0280008108.01.T01:intron 15.0%
!!! TTTTGTACTAAAATTTTCTT+TGG + Chr2:25571023-25571042 MsG0280008108.01.T01:intron 15.0%
!! AAATTATCGTAATCAACTAT+TGG + Chr2:25570759-25570778 MsG0280008108.01.T01:intron 20.0%
!! AAGAAAACTACTTGATATAA+AGG - Chr2:25572584-25572603 None:intergenic 20.0%
!! AATAGTTGATTACGATAATT+TGG - Chr2:25570760-25570779 None:intergenic 20.0%
!! AATATATTCTGCATTGAATA+TGG + Chr2:25571052-25571071 MsG0280008108.01.T01:intron 20.0%
!! AATGATTAAAAGAAAGAGAA+TGG - Chr2:25571495-25571514 None:intergenic 20.0%
!! ACAATTGGAACATTATTATT+AGG - Chr2:25573003-25573022 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + Chr2:25570857-25570876 MsG0280008108.01.T01:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - Chr2:25570876-25570857 None:intergenic 20.0%
!! ATAGATTTCATACCTAATTT+AGG - Chr2:25570646-25570665 None:intergenic 20.0%
!! CAATTGGAACATTATTATTA+GGG - Chr2:25573002-25573021 None:intergenic 20.0%
!! CACCTAACAATAATAAATTA+AGG + Chr2:25571171-25571190 MsG0280008108.01.T01:intron 20.0%
!! CACCTTAATTTATTATTGTT+AGG - Chr2:25571176-25571195 None:intergenic 20.0%
!! CTAACAATAATAAATTAAGG+TGG + Chr2:25571174-25571193 MsG0280008108.01.T01:intron 20.0%
!! TTCCAATTGTAAAATTAGAT+GGG + Chr2:25573013-25573032 MsG0280008108.01.T01:CDS 20.0%
!!! AAGAATTGATAATTGTTACA+TGG + Chr2:25572834-25572853 MsG0280008108.01.T01:CDS 20.0%
!!! AATGAGATTTTTGAAAATTG+TGG + Chr2:25571830-25571849 MsG0280008108.01.T01:intron 20.0%
!!! AGATATATTGAACTCTTTTT+TGG + Chr2:25572194-25572213 MsG0280008108.01.T01:intron 20.0%
!!! ATGAGATTTTTGAAAATTGT+GGG + Chr2:25571831-25571850 MsG0280008108.01.T01:intron 20.0%
!!! ATTCAATGCAGAATATATTT+TGG - Chr2:25571051-25571070 None:intergenic 20.0%
!!! ATTCATTTTATACATTAGAG+GGG + Chr2:25573199-25573218 MsG0280008108.01.T01:CDS 20.0%
!!! ATTGTTTTGAAGTCAATTAA+AGG + Chr2:25572931-25572950 MsG0280008108.01.T01:CDS 20.0%
!!! GCATTTGATATTGATAATTT+AGG + Chr2:25570328-25570347 MsG0280008108.01.T01:CDS 20.0%
!!! GTATTCATTTTATACATTAG+AGG + Chr2:25573197-25573216 MsG0280008108.01.T01:CDS 20.0%
!!! TAAAAGTAATACTTTTGAAG+TGG - Chr2:25571432-25571451 None:intergenic 20.0%
!!! TATACTTTTGAAAACAAGTT+TGG + Chr2:25572030-25572049 MsG0280008108.01.T01:intron 20.0%
!!! TTACTAATGGTTTTTTCTTA+GGG + Chr2:25572899-25572918 MsG0280008108.01.T01:CDS 20.0%
!!! TTGTTTTGAAGTCAATTAAA+GGG + Chr2:25572932-25572951 MsG0280008108.01.T01:CDS 20.0%
!!! TTTATATCAAGTAGTTTTCT+TGG + Chr2:25572583-25572602 MsG0280008108.01.T01:CDS 20.0%
!!! TTTTACTATTTTCATTTCTC+CGG - Chr2:25571794-25571813 None:intergenic 20.0%
! AAATTGAGAAACAATGTGAT+TGG + Chr2:25571657-25571676 MsG0280008108.01.T01:intron 25.0%
! ATTGATATGTAATGTGAGTA+TGG + Chr2:25570105-25570124 MsG0280008108.01.T01:intron 25.0%
! ATTTAGTAACATTGATCAAG+TGG + Chr2:25573038-25573057 MsG0280008108.01.T01:CDS 25.0%
! GAAGGAATTAAAGTTAAAAG+TGG + Chr2:25572550-25572569 MsG0280008108.01.T01:CDS 25.0%
! GAATTACTTCAACATACAAT+AGG + Chr2:25572400-25572419 MsG0280008108.01.T01:CDS 25.0%
! GGATTATAATTCTATAGTCA+TGG + Chr2:25572855-25572874 MsG0280008108.01.T01:CDS 25.0%
! GTTCCAATTGTAAAATTAGA+TGG + Chr2:25573012-25573031 MsG0280008108.01.T01:CDS 25.0%
! TACTTGATATAAAGGTTTCA+TGG - Chr2:25572576-25572595 None:intergenic 25.0%
! TCAATTAAAGGGAAAGTTTA+TGG + Chr2:25572943-25572962 MsG0280008108.01.T01:CDS 25.0%
! TCCAATTGTAAAATTAGATG+GGG + Chr2:25573014-25573033 MsG0280008108.01.T01:CDS 25.0%
! TGAAAATGACTAATCCTATT+AGG - Chr2:25573177-25573196 None:intergenic 25.0%
!! ATTCAACAAAAGTACAAAAC+CGG + Chr2:25571772-25571791 MsG0280008108.01.T01:intron 25.0%
!! CTGATTTTCCAAAAAGAAAA+GGG - Chr2:25570543-25570562 None:intergenic 25.0%
!! GAATTTTGTTGAAATTAGCA+GGG + Chr2:25572800-25572819 MsG0280008108.01.T01:CDS 25.0%
!! TATGTCCAAAAGTATAGTTT+CGG - Chr2:25571983-25572002 None:intergenic 25.0%
!! TCTGATTTTCCAAAAAGAAA+AGG - Chr2:25570544-25570563 None:intergenic 25.0%
!!! ACAAGTTTTGTTTAAGAAGA+TGG - Chr2:25572249-25572268 None:intergenic 25.0%
!!! ATGTTTTGGTATTTCCTAAT+AGG + Chr2:25573160-25573179 MsG0280008108.01.T01:CDS 25.0%
!!! GGTGTTATTATTGATTTTCA+AGG + Chr2:25570385-25570404 MsG0280008108.01.T01:CDS 25.0%
!!! GTTACTAATGGTTTTTTCTT+AGG + Chr2:25572898-25572917 MsG0280008108.01.T01:CDS 25.0%
!!! GTTATTATTGATTTTCAAGG+TGG + Chr2:25570388-25570407 MsG0280008108.01.T01:CDS 25.0%
!!! TACTAATGGTTTTTTCTTAG+GGG + Chr2:25572900-25572919 MsG0280008108.01.T01:CDS 25.0%
!!! TGGTATGATTTTAAGATCAA+CGG + Chr2:25573083-25573102 MsG0280008108.01.T01:CDS 25.0%
AAATGTATTGGAAGCTCTTA+AGG - Chr2:25572302-25572321 None:intergenic 30.0%
AACTCATTGTTCAACAAAGA+AGG - Chr2:25573310-25573329 None:intergenic 30.0%
AAGTGATAAGAGAAATGCTA+AGG - Chr2:25569792-25569811 None:intergenic 30.0%
AATCAATAATAACACCTCCA+AGG - Chr2:25570381-25570400 None:intergenic 30.0%
ATATTTGACTGTAATCAGAG+TGG + Chr2:25570220-25570239 MsG0280008108.01.T01:intron 30.0%
ATGAAATGTTTGAAGGTGAA+AGG + Chr2:25572271-25572290 MsG0280008108.01.T01:intron 30.0%
ATTGATAGGAACAATGTGAT+TGG + Chr2:25573063-25573082 MsG0280008108.01.T01:CDS 30.0%
CAAATGCATCAAAGTAGTAA+AGG - Chr2:25570515-25570534 None:intergenic 30.0%
CATTGTTATAACAAAGCAAC+AGG + Chr2:25572763-25572782 MsG0280008108.01.T01:CDS 30.0%
CCAATTGTAAAATTAGATGG+GGG + Chr2:25573015-25573034 MsG0280008108.01.T01:CDS 30.0%
CTTGTACATGAAATGTTTGA+AGG + Chr2:25572264-25572283 MsG0280008108.01.T01:intron 30.0%
GAAATCTATTACTACCATGT+TGG - Chr2:25571631-25571650 None:intergenic 30.0%
GTTATTAAGAATTTGCAGGA+TGG - Chr2:25571134-25571153 None:intergenic 30.0%
TAATTCCTTCTGTGTTGAAA+TGG - Chr2:25572540-25572559 None:intergenic 30.0%
TCTATCAGTTGGAAATGTAT+TGG - Chr2:25572314-25572333 None:intergenic 30.0%
TGGAGTTATTAAGAATTTGC+AGG - Chr2:25571138-25571157 None:intergenic 30.0%
TTAGGTGAGACTTAAATTGA+TGG - Chr2:25571158-25571177 None:intergenic 30.0%
TTAGTAACATGAACTTGAAC+AGG - Chr2:25572886-25572905 None:intergenic 30.0%
TTCTTTGTTGAACAATGAGT+TGG + Chr2:25573309-25573328 MsG0280008108.01.T01:exon 30.0%
TTGTTTAAGAAGATGGAACT+TGG - Chr2:25572242-25572261 None:intergenic 30.0%
! AGCATTTCTCTTATCACTTT+TGG + Chr2:25569793-25569812 MsG0280008108.01.T01:CDS 30.0%
! CAAAAGTATACAGCATGATT+GGG - Chr2:25572020-25572039 None:intergenic 30.0%
! GGAATTTTGTTGAAATTAGC+AGG + Chr2:25572799-25572818 MsG0280008108.01.T01:CDS 30.0%
! GTATGCAGAGTATTAATTGA+GGG - Chr2:25571410-25571429 None:intergenic 30.0%
! GTTCAAGTTCATGTTACTAA+TGG + Chr2:25572886-25572905 MsG0280008108.01.T01:CDS 30.0%
! TACCATGTTGGAAATTTTGA+GGG - Chr2:25571619-25571638 None:intergenic 30.0%
! TATAACAAAGCAACAGGTTT+TGG + Chr2:25572769-25572788 MsG0280008108.01.T01:CDS 30.0%
! TCAAAAGTATACAGCATGAT+TGG - Chr2:25572021-25572040 None:intergenic 30.0%
! TTGTTGATCCCTTTTCTTTT+TGG + Chr2:25570532-25570551 MsG0280008108.01.T01:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG + Chr2:25570923-25570942 MsG0280008108.01.T01:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG - Chr2:25570942-25570923 None:intergenic 30.0%
!! ATACTTCTGAAGTGTATTCA+AGG - Chr2:25571930-25571949 None:intergenic 30.0%
!! GGGTGATTTTTCAAATGTTT+TGG + Chr2:25573146-25573165 MsG0280008108.01.T01:CDS 30.0%
!! TACTTCTGAAGTGTATTCAA+GGG - Chr2:25571929-25571948 None:intergenic 30.0%
!! TTATAGAAGTGTCTGGTATA+CGG + Chr2:25572055-25572074 MsG0280008108.01.T01:intron 30.0%
!!! ATGATTTTAAGATCAACGGT+TGG + Chr2:25573087-25573106 MsG0280008108.01.T01:CDS 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + Chr2:25571311-25571330 MsG0280008108.01.T01:intron 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - Chr2:25571330-25571311 None:intergenic 30.0%
!!! TTTGGTTTTATAGAAGTGTC+TGG + Chr2:25572048-25572067 MsG0280008108.01.T01:intron 30.0%
AAAATTGTGGGTGCAAAAGA+CGG + Chr2:25571843-25571862 MsG0280008108.01.T01:intron 35.0%
AAATGTTTGAAGGTGAAAGG+AGG + Chr2:25572274-25572293 MsG0280008108.01.T01:intron 35.0%
AACAGATGAAGGAAATGTGA+TGG - Chr2:25570434-25570453 None:intergenic 35.0%
AAGTTGAATGTGGATGGTAA+TGG + Chr2:25573111-25573130 MsG0280008108.01.T01:CDS 35.0%
ACAGATGAAGGAAATGTGAT+GGG - Chr2:25570433-25570452 None:intergenic 35.0%
ACTGAATGAGCAACAAACTT+TGG - Chr2:25573225-25573244 None:intergenic 35.0%
AGCTTCTACAGCTTTCATAA+TGG - Chr2:25570308-25570327 None:intergenic 35.0%
AGGGAGTAAATTGGACAATA+TGG + Chr2:25571567-25571586 MsG0280008108.01.T01:intron 35.0%
ATCACATTTCCTTCATCTGT+TGG + Chr2:25570433-25570452 MsG0280008108.01.T01:CDS 35.0%
ATGCATCAAAGTAGTAAAGG+AGG - Chr2:25570512-25570531 None:intergenic 35.0%
ATGGAAACTTCCAAAGCTTA+TGG + Chr2:25571586-25571605 MsG0280008108.01.T01:intron 35.0%
CAAAGTAGTAAAGGAGGAAA+AGG - Chr2:25570506-25570525 None:intergenic 35.0%
GAGAAACAATGTGATTGGAA+CGG + Chr2:25571662-25571681 MsG0280008108.01.T01:intron 35.0%
GATCTGCACCATACTTAATT+GGG - Chr2:25570258-25570277 None:intergenic 35.0%
TACCCTCAAAATTTCCAACA+TGG + Chr2:25571614-25571633 MsG0280008108.01.T01:intron 35.0%
TAGCAATGACAATGCTATCA+CGG + Chr2:25572672-25572691 MsG0280008108.01.T01:CDS 35.0%
TTAAGAAGATGGAACTTGGA+AGG - Chr2:25572238-25572257 None:intergenic 35.0%
TTAGGGCTTGAATTATTACC+AGG + Chr2:25570346-25570365 MsG0280008108.01.T01:CDS 35.0%
TTCCTTAACATGTGTGCAAT+TGG - Chr2:25570703-25570722 None:intergenic 35.0%
TTGATCAAGTGGTGATTGAT+AGG + Chr2:25573049-25573068 MsG0280008108.01.T01:CDS 35.0%
! AAAGGCGAAAGACAATTTTC+GGG + Chr2:25572631-25572650 MsG0280008108.01.T01:CDS 35.0%
! ATCGTCCGAAACTATACTTT+TGG + Chr2:25571975-25571994 MsG0280008108.01.T01:intron 35.0%
! CAAAGGCGAAAGACAATTTT+CGG + Chr2:25572630-25572649 MsG0280008108.01.T01:CDS 35.0%
! CCCCCATCTAATTTTACAAT+TGG - Chr2:25573018-25573037 None:intergenic 35.0%
! CTACCATGTTGGAAATTTTG+AGG - Chr2:25571620-25571639 None:intergenic 35.0%
! GGTATGCAGAGTATTAATTG+AGG - Chr2:25571411-25571430 None:intergenic 35.0%
! TCAACAAGGTATCTATCAGT+TGG - Chr2:25572325-25572344 None:intergenic 35.0%
! TGTATGCTGTTGTTGCATTT+TGG - Chr2:25572375-25572394 None:intergenic 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - Chr2:25570677-25570696 None:intergenic 35.0%
!! ACTTCTGAAGTGTATTCAAG+GGG - Chr2:25571928-25571947 None:intergenic 35.0%
!! TGTTGAACAATGAGTTGGTA+TGG + Chr2:25573314-25573333 MsG0280008108.01.T01:exon 35.0%
!!! TGTTGCATTTTGGAGTACTT+TGG - Chr2:25572365-25572384 None:intergenic 35.0%
AAAGCCTTGACCAAAATGTC+TGG + Chr2:25571230-25571249 MsG0280008108.01.T01:intron 40.0%
AAATTGTGGGTGCAAAAGAC+GGG + Chr2:25571844-25571863 MsG0280008108.01.T01:intron 40.0%
AACGGTTGGAAAGTTGAATG+TGG + Chr2:25573101-25573120 MsG0280008108.01.T01:CDS 40.0%
AAGGAAACAACACCATGAAC+TGG - Chr2:25573291-25573310 None:intergenic 40.0%
ACAAGATTGCCAACAGATGA+AGG - Chr2:25570445-25570464 None:intergenic 40.0%
ACCATCCTCACACACAAAAA+GGG + Chr2:25569931-25569950 MsG0280008108.01.T01:CDS 40.0%
AGCCAATTGCACACATGTTA+AGG + Chr2:25570698-25570717 MsG0280008108.01.T01:intron 40.0%
GAAACTTCCAAAGCTTATGG+TGG + Chr2:25571589-25571608 MsG0280008108.01.T01:intron 40.0%
GAAAGTGAGAAACCAGTTCA+TGG + Chr2:25573276-25573295 MsG0280008108.01.T01:CDS 40.0%
GGACAAGCTAACATAATCAC+AGG + Chr2:25572736-25572755 MsG0280008108.01.T01:CDS 40.0%
GGATCTGCACCATACTTAAT+TGG - Chr2:25570259-25570278 None:intergenic 40.0%
GGGACTATGTAAGAGCAAAA+TGG + Chr2:25571864-25571883 MsG0280008108.01.T01:intron 40.0%
GTGGTGTACCCAATTAAGTA+TGG + Chr2:25570247-25570266 MsG0280008108.01.T01:CDS 40.0%
GTTGGAAAGTTGAATGTGGA+TGG + Chr2:25573105-25573124 MsG0280008108.01.T01:CDS 40.0%
TAAGGTGGCCAACAAGATAT+GGG + Chr2:25571189-25571208 MsG0280008108.01.T01:intron 40.0%
TACGTGTTCATTGAAGCAAG+TGG - Chr2:25569831-25569850 None:intergenic 40.0%
TTAAGGTGGCCAACAAGATA+TGG + Chr2:25571188-25571207 MsG0280008108.01.T01:intron 40.0%
TTACCAGGAATTAGAGACCT+TGG + Chr2:25570361-25570380 MsG0280008108.01.T01:CDS 40.0%
TTCATCTGTTGGCAATCTTG+TGG + Chr2:25570444-25570463 MsG0280008108.01.T01:CDS 40.0%
TTCCTCCATTTCAACACAGA+AGG + Chr2:25572532-25572551 MsG0280008108.01.T01:CDS 40.0%
TTCCTTCTGTGTTGAAATGG+AGG - Chr2:25572537-25572556 None:intergenic 40.0%
TTGAAACGGAGGGAGTAAAT+TGG + Chr2:25571558-25571577 MsG0280008108.01.T01:intron 40.0%
TTGTTGCACACAACTCAACA+AGG - Chr2:25572339-25572358 None:intergenic 40.0%
! ACATACCCTTTTTGTGTGTG+AGG - Chr2:25569939-25569958 None:intergenic 40.0%
! AGCCATTGGAATTGCTGTTA+GGG + Chr2:25572714-25572733 MsG0280008108.01.T01:CDS 40.0%
! AGGGTTTTCCCATATCTTGT+TGG - Chr2:25571200-25571219 None:intergenic 40.0%
! CAATTGCTAGCCAGACATTT+TGG - Chr2:25571243-25571262 None:intergenic 40.0%
!! AACAAAGCAACAGGTTTTGG+TGG + Chr2:25572772-25572791 MsG0280008108.01.T01:CDS 40.0%
!! AGACATTTTGGTCAAGGCTT+TGG - Chr2:25571231-25571250 None:intergenic 40.0%
!! GGATGGTAATGGCACAAAAT+GGG + Chr2:25573122-25573141 MsG0280008108.01.T01:CDS 40.0%
!! TGGATGGTAATGGCACAAAA+TGG + Chr2:25573121-25573140 MsG0280008108.01.T01:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + Chr2:25570927-25570946 MsG0280008108.01.T01:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - Chr2:25570946-25570927 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+TGG - Chr2:25570946-25570927 None:intergenic 40.0%
AAGTATGGTGCAGATCCAAC+AGG + Chr2:25570262-25570281 MsG0280008108.01.T01:CDS 45.0%
AGTATGGTGCAGATCCAACA+GGG + Chr2:25570263-25570282 MsG0280008108.01.T01:CDS 45.0%
CACCATCCTCACACACAAAA+AGG + Chr2:25569930-25569949 MsG0280008108.01.T01:CDS 45.0%
CTGTAATCAGAGTGGAAGAG+TGG + Chr2:25570228-25570247 MsG0280008108.01.T01:intron 45.0%
CTTGGACAACACTCAACAGT+CGG + Chr2:25572601-25572620 MsG0280008108.01.T01:CDS 45.0%
TCCCCTAACAGCAATTCCAA+TGG - Chr2:25572719-25572738 None:intergenic 45.0%
TGTCTGGCTAGCAATTGTGT+TGG + Chr2:25571246-25571265 MsG0280008108.01.T01:intron 45.0%
TTTGTGTGTGAGGATGGTGA+TGG - Chr2:25569929-25569948 None:intergenic 45.0%
! ACCCTTTTTGTGTGTGAGGA+TGG - Chr2:25569935-25569954 None:intergenic 45.0%
! CAAGGCTTTGGTGTACTCAA+GGG - Chr2:25571219-25571238 None:intergenic 45.0%
! CAGCCATTGGAATTGCTGTT+AGG + Chr2:25572713-25572732 MsG0280008108.01.T01:CDS 45.0%
! CTGCTTTCATTTTCCCCTGT+TGG - Chr2:25570280-25570299 None:intergenic 45.0%
! GCAATTTTCTCAGCAGCCAT+TGG + Chr2:25572700-25572719 MsG0280008108.01.T01:CDS 45.0%
! GCCATTGGAATTGCTGTTAG+GGG + Chr2:25572715-25572734 MsG0280008108.01.T01:CDS 45.0%
! GGTAATGGCACAAAATGGGT+GGG + Chr2:25573126-25573145 MsG0280008108.01.T01:CDS 45.0%
! TAGTGAGCTAGAAGCGTACT+AGG + Chr2:25571102-25571121 MsG0280008108.01.T01:intron 45.0%
! TCAAGGCTTTGGTGTACTCA+AGG - Chr2:25571220-25571239 None:intergenic 45.0%
!! CTAGCCAGACATTTTGGTCA+AGG - Chr2:25571237-25571256 None:intergenic 45.0%
!! GCAACAGGTTTTGGTGGAAT+TGG + Chr2:25572778-25572797 MsG0280008108.01.T01:CDS 45.0%
!! TGGTAATGGCACAAAATGGG+TGG + Chr2:25573125-25573144 MsG0280008108.01.T01:CDS 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + Chr2:25570926-25570945 MsG0280008108.01.T01:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - Chr2:25570945-25570926 None:intergenic 45.0%
CCAGGAATTAGAGACCTTGG+AGG + Chr2:25570364-25570383 MsG0280008108.01.T01:CDS 50.0%
CCTCCAAGGTCTCTAATTCC+TGG - Chr2:25570367-25570386 None:intergenic 50.0%
CGATGAGTATTCCTCCACCT+CGG - Chr2:25572477-25572496 None:intergenic 50.0%
GGGTAGTCCACCATAAGCTT+TGG - Chr2:25571599-25571618 None:intergenic 50.0%
GTATGGTGCAGATCCAACAG+GGG + Chr2:25570264-25570283 MsG0280008108.01.T01:CDS 50.0%
!! GATGGTGATGGAGAAGGAGA+GGG - Chr2:25569917-25569936 None:intergenic 50.0%
!! TGTGAGGATGGTGATGGAGA+AGG - Chr2:25569923-25569942 None:intergenic 50.0%
AGGAGAGGGTGGTTGTGACA+AGG - Chr2:25569903-25569922 None:intergenic 55.0%
GGACAACACTCAACAGTCGG+CGG + Chr2:25572604-25572623 MsG0280008108.01.T01:CDS 55.0%
TCAACAGTCGGCGGAGACAA+AGG + Chr2:25572613-25572632 MsG0280008108.01.T01:CDS 55.0%
! CTCTTCGATTCGAGCTACCG+AGG + Chr2:25572457-25572476 MsG0280008108.01.T01:CDS 55.0%
! TTCGATTCGAGCTACCGAGG+TGG + Chr2:25572460-25572479 MsG0280008108.01.T01:CDS 55.0%
!! GGATGGTGATGGAGAAGGAG+AGG - Chr2:25569918-25569937 None:intergenic 55.0%
GATTCGAGCTACCGAGGTGG+AGG + Chr2:25572463-25572482 MsG0280008108.01.T01:CDS 60.0%
!! GGTGATGGAGAAGGAGAGGG+TGG - Chr2:25569914-25569933 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 25569767 25573351 25569767 ID=MsG0280008108.01;Name=MsG0280008108.01
Chr2 mRNA 25569767 25573351 25569767 ID=MsG0280008108.01.T01;Parent=MsG0280008108.01;Name=MsG0280008108.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0.5|0.33|1|0.5|0.66|3|25|484
Chr2 exon 25569767 25569952 25569767 ID=MsG0280008108.01.T01:exon:14053;Parent=MsG0280008108.01.T01
Chr2 exon 25570238 25570465 25570238 ID=MsG0280008108.01.T01:exon:14054;Parent=MsG0280008108.01.T01
Chr2 exon 25572286 25573351 25572286 ID=MsG0280008108.01.T01:exon:14055;Parent=MsG0280008108.01.T01
Chr2 CDS 25569767 25569952 25569767 ID=MsG0280008108.01.T01:cds;Parent=MsG0280008108.01.T01
Chr2 CDS 25570238 25570465 25570238 ID=MsG0280008108.01.T01:cds;Parent=MsG0280008108.01.T01
Chr2 CDS 25572286 25573326 25572286 ID=MsG0280008108.01.T01:cds;Parent=MsG0280008108.01.T01
Chr2 three_prime_UTR 25573327 25573351 25573327 ID=MsG0280008108.01.T01:three_prime_utr;Parent=MsG0280008108.01.T01
Gene Sequence

>MsG0280008108.01.T01

ATGTTTGCTATGAGATTCTCAGCCTTAGCATTTCTCTTATCACTTTTGGTGATACAAGAAACCACTTGCTTCAATGAACACGTAGAACTTTCTCATCTCATGAAAAAAATTGCTAAGCATAAAGTAGACATTGCCTTGTCACAACCACCCTCTCCTTCTCCATCACCATCCTCACACACAAAAAGGAGTGGAAGAGTGGTGTACCCAATTAAGTATGGTGCAGATCCAACAGGGGAAAATGAAAGCAGTGATTCCATTATGAAAGCTGTAGAAGCTGCATTTGATATTGATAATTTAGGGCTTGAATTATTACCAGGAATTAGAGACCTTGGAGGTGTTATTATTGATTTTCAAGGTGGAAACTACAAAATCAGCAATCCCATCACATTTCCTTCATCTGTTGGCAATCTTGTGGTGAAAGGAGGAACCTTAAGAGCTTCCAATACATTTCCAACTGATAGATACCTTGTTGAGTTGTGTGCAACAAATTCCAAAGTACTCCAAAATGCAACAACAGCATACAACAACGAATTACTTCAACATACAATAGGAATTTACTATGAAGACATAACATTTAGAGACATACTCTTCGATTCGAGCTACCGAGGTGGAGGAATACTCATCGTCGATTCAGCTAGAATCAGAATCGACAATTGTTTCTTCCTCCATTTCAACACAGAAGGAATTAAAGTTAAAAGTGGCCATGAAACCTTTATATCAAGTAGTTTTCTTGGACAACACTCAACAGTCGGCGGAGACAAAGGCGAAAGACAATTTTCGGGAACTGCAATTGATCTTGCTAGCAATGACAATGCTATCACGGATGTCGCAATTTTCTCAGCAGCCATTGGAATTGCTGTTAGGGGACAAGCTAACATAATCACAGGTGTGCATTGTTATAACAAAGCAACAGGTTTTGGTGGAATTGGAATTTTGTTGAAATTAGCAGGGAATTCACAAACAAGAATTGATAATTGTTACATGGATTATAATTCTATAGTCATGGAAGATCCTGTTCAAGTTCATGTTACTAATGGTTTTTTCTTAGGGGATGCTAATATTGTTTTGAAGTCAATTAAAGGGAAAGTTTATGGTTTAAATATTGTTAATAATATGTTTAGTGGAAACCCTAATAATAATGTTCCAATTGTAAAATTAGATGGGGGATTTAGTAACATTGATCAAGTGGTGATTGATAGGAACAATGTGATTGGTATGATTTTAAGATCAACGGTTGGAAAGTTGAATGTGGATGGTAATGGCACAAAATGGGTGGGTGATTTTTCAAATGTTTTGGTATTTCCTAATAGGATTAGTCATTTTCAGTATTCATTTTATACATTAGAGGGGCCAAAGTTTGTTGCTCATTCAGTGAGTAATGTGTCAAATAATGTTGTAGTTGTTGAAAGTGAGAAACCAGTTCATGGTGTTGTTTCCTTCTTTGTTGAACAATGA
Protein sequence

>MsG0280008108.01.T01

MFAMRFSALAFLLSLLVIQETTCFNEHVELSHLMKKIAKHKVDIALSQPPSPSPSPSSHTKRSGRVVYPIKYGADPTGENESSDSIMKAVEAAFDIDNLGLELLPGIRDLGGVIIDFQGGNYKISNPITFPSSVGNLVVKGGTLRASNTFPTDRYLVELCATNSKVLQNATTAYNNELLQHTIGIYYEDITFRDILFDSSYRGGGILIVDSARIRIDNCFFLHFNTEGIKVKSGHETFISSSFLGQHSTVGGDKGERQFSGTAIDLASNDNAITDVAIFSAAIGIAVRGQANIITGVHCYNKATGFGGIGILLKLAGNSQTRIDNCYMDYNSIVMEDPVQVHVTNGFFLGDANIVLKSIKGKVYGLNIVNNMFSGNPNNNVPIVKLDGGFSNIDQVVIDRNNVIGMILRSTVGKLNVDGNGTKWVGDFSNVLVFPNRISHFQYSFYTLEGPKFVAHSVSNVSNNVVVVESEKPVHGVVSFFVEQ*