AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008185.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008185.01.T01 MTR_0443s0040 87.500 312 36 2 1 311 1 310 0.0 566
MsG0280008185.01.T01 MTR_0062s0170 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_0062s0110 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_0062s0130 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_2g036240 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_2g036260 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_2g036290 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_8g046730 58.333 312 121 6 4 311 3 309 8.12e-112 326
MsG0280008185.01.T01 MTR_0062s0020 47.883 307 137 7 14 308 2 297 9.09e-91 273
MsG0280008185.01.T01 MTR_0002s1060 48.675 302 138 4 12 310 12 299 1.30e-90 272
MsG0280008185.01.T01 MTR_0062s0090 47.213 305 136 10 16 308 4 295 1.83e-79 244
MsG0280008185.01.T01 MTR_0062s0040 47.213 305 136 10 16 308 4 295 1.83e-79 244
MsG0280008185.01.T01 MTR_0062s0060 47.213 305 136 10 16 308 4 295 1.83e-79 244
MsG0280008185.01.T01 MTR_0002s0040 43.289 298 148 7 14 309 13 291 1.62e-74 231
MsG0280008185.01.T01 MTR_0263s0020 43.537 294 145 7 14 305 13 287 9.65e-72 224
MsG0280008185.01.T01 MTR_1457s0010 38.934 244 116 5 69 311 1 212 8.42e-45 152
MsG0280008185.01.T01 MTR_6g079630 31.317 281 158 9 16 287 34 288 1.47e-31 120
MsG0280008185.01.T01 MTR_2g096030 32.270 282 154 10 16 287 31 285 3.39e-29 113
MsG0280008185.01.T01 MTR_2g096040 30.909 275 155 11 15 279 3 252 2.44e-28 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAAATCATCTAAAGTTTC+TGG 0.145221 2:+26897933 None:intergenic
AATCAATTCCACTTCATTCT+AGG 0.205618 2:-26897885 MsG0280008185.01.T01:CDS
CCCACTACCACAAATTGTTT+TGG 0.245343 2:-26897419 MsG0280008185.01.T01:CDS
TGTAGCTAGTAATCAATTAT+TGG 0.294200 2:+26897086 None:intergenic
ATTCAATCCTAAGGAGAAAA+AGG 0.297710 2:-26897703 MsG0280008185.01.T01:CDS
GGACACATATTCCATGGATT+TGG 0.302531 2:+26897467 None:intergenic
TTACGGTGAGCTTAAACTTT+TGG 0.347612 2:-26897295 MsG0280008185.01.T01:CDS
GATATGTCTGATCGAGCTTA+TGG 0.361529 2:+26897665 None:intergenic
CCCAAAACAATTTGTGGTAG+TGG 0.365132 2:+26897418 None:intergenic
TACCTTGTCAATGATGATTC+TGG 0.380807 2:-26897624 MsG0280008185.01.T01:CDS
CGGGACATTTCGTTCGCGAT+TGG 0.385507 2:-26897827 MsG0280008185.01.T01:CDS
ACAATTTGTGGTAGTGGGCT+TGG 0.408360 2:+26897424 None:intergenic
CACTGCACTAGCATAATTGA+TGG 0.434540 2:-26897594 MsG0280008185.01.T01:CDS
AATCAACTAATAACGGATTA+CGG 0.451454 2:-26897312 MsG0280008185.01.T01:CDS
ATCATCAAACTCATCCTTTG+AGG 0.453286 2:+26897341 None:intergenic
CGGTTCATCTTAGTATCAGC+TGG 0.454457 2:+26897255 None:intergenic
GAAAATCATCTAAAGTTTCT+GGG 0.456174 2:+26897934 None:intergenic
TCATCATTGATGATCTCAAC+AGG 0.458322 2:+26897912 None:intergenic
AGCATTTCCTCCAAGATGTA+AGG 0.467593 2:+26897123 None:intergenic
TGTGGTCCCAAAACAATTTG+TGG 0.468651 2:+26897412 None:intergenic
GATGTCATTGCTCGGTGGGT+TGG 0.481116 2:+26897149 None:intergenic
TATATCGATTCTAGTGTGAC+TGG 0.486159 2:-26897552 MsG0280008185.01.T01:CDS
GCCACTGAGGTGTATAAAGA+TGG 0.489071 2:-26897858 MsG0280008185.01.T01:CDS
AACCGAGAGGTGTTCATTGA+GGG 0.499384 2:-26897237 MsG0280008185.01.T01:CDS
CGCCCTCAATGAACACCTCT+CGG 0.504404 2:+26897235 None:intergenic
TGACATCGAGCCTTACATCT+TGG 0.507062 2:-26897133 MsG0280008185.01.T01:CDS
TCTCTACAATCAACTAATAA+CGG 0.510522 2:-26897319 MsG0280008185.01.T01:CDS
CCAAAACAATTTGTGGTAGT+GGG 0.514829 2:+26897419 None:intergenic
TATAAAGATGGAAAAGGAAC+GGG 0.518546 2:-26897846 MsG0280008185.01.T01:CDS
CTAGTAATCAATTATTGGTA+AGG 0.518596 2:+26897091 None:intergenic
ATCAACAGCAAATCACTCCT+TGG 0.519679 2:-26897201 MsG0280008185.01.T01:CDS
TAAATATCCATTCAATCCTA+AGG 0.520789 2:-26897712 MsG0280008185.01.T01:CDS
GCTGACTTCTCCAACTGCAT+TGG 0.521072 2:-26897528 MsG0280008185.01.T01:CDS
AATAACTTGTCCAATGCAGT+TGG 0.521593 2:+26897518 None:intergenic
ATTTGTGGTAGTGGGCTTGG+AGG 0.527426 2:+26897427 None:intergenic
GCTCGATGTCATTGCTCGGT+GGG 0.532171 2:+26897145 None:intergenic
GAGGAGCTCTGTCGGAGCAA+TGG 0.538177 2:+26897446 None:intergenic
ATGAAGGTTATGATAACTAT+CGG 0.552996 2:-26897747 MsG0280008185.01.T01:CDS
CATCGAGCCTTACATCTTGG+AGG 0.554478 2:-26897130 MsG0280008185.01.T01:CDS
TCGAGAATACATTGGCGTGA+AGG 0.556428 2:-26897961 MsG0280008185.01.T01:CDS
GAAAGAACATAAGAATATGA+AGG 0.556511 2:-26897763 MsG0280008185.01.T01:CDS
TAAGCATGTATCCAAATCCA+TGG 0.557313 2:-26897478 MsG0280008185.01.T01:CDS
CATCTTAGTATCAGCTGGAT+CGG 0.557541 2:+26897260 None:intergenic
TGAATGGATATTTAGTGCCA+TGG 0.560003 2:+26897721 None:intergenic
GAGGTGTATAAAGATGGAAA+AGG 0.564302 2:-26897852 MsG0280008185.01.T01:CDS
GAACCGAGAGGTGTTCATTG+AGG 0.566347 2:-26897238 MsG0280008185.01.T01:CDS
CAATCAAACTATATCCTCAA+AGG 0.567378 2:-26897355 MsG0280008185.01.T01:CDS
AGCAATGGACACATATTCCA+TGG 0.567858 2:+26897461 None:intergenic
GGCTCGATGTCATTGCTCGG+TGG 0.574776 2:+26897144 None:intergenic
ATATCGATTCTAGTGTGACT+GGG 0.576037 2:-26897551 MsG0280008185.01.T01:CDS
TGGGCTTGGAGGAGCTCTGT+CGG 0.577415 2:+26897438 None:intergenic
ATGATAACTATCGGAGGCCA+TGG 0.579102 2:-26897738 MsG0280008185.01.T01:CDS
TAAGGCTCGATGTCATTGCT+CGG 0.600452 2:+26897141 None:intergenic
AGTTATTAAACATCTCAAGA+AGG 0.601516 2:-26897502 MsG0280008185.01.T01:CDS
GTATAAAGATGGAAAAGGAA+CGG 0.613441 2:-26897847 MsG0280008185.01.T01:CDS
TGGCGAAGCCTAGAATGAAG+TGG 0.614487 2:+26897877 None:intergenic
TACCAGAATCATCATTGACA+AGG 0.665409 2:+26897622 None:intergenic
TCCATCTTTATACACCTCAG+TGG 0.670679 2:+26897857 None:intergenic
CATCAATTATGCTAGTGCAG+TGG 0.677859 2:+26897595 None:intergenic
CATTCTAGGCTTCGCCACTG+AGG 0.701525 2:-26897871 MsG0280008185.01.T01:CDS
AAGGTTATGATAACTATCGG+AGG 0.701816 2:-26897744 MsG0280008185.01.T01:CDS
TATCGATTCTAGTGTGACTG+GGG 0.708158 2:-26897550 MsG0280008185.01.T01:CDS
TGATACTAAGATGAACCGAG+AGG 0.777827 2:-26897250 MsG0280008185.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTTATTAAACATCTCAAGA+AGG - Chr2:26897607-26897626 MsG0280008185.01.T01:CDS 25.0%
! ATGAAGGTTATGATAACTAT+CGG - Chr2:26897362-26897381 MsG0280008185.01.T01:CDS 25.0%
! ATGTATTCTCGAAAAATTGT+TGG + Chr2:26897140-26897159 None:intergenic 25.0%
! GAAAGAACATAAGAATATGA+AGG - Chr2:26897346-26897365 MsG0280008185.01.T01:CDS 25.0%
! TAAATATCCATTCAATCCTA+AGG - Chr2:26897397-26897416 MsG0280008185.01.T01:CDS 25.0%
! TCTCTACAATCAACTAATAA+CGG - Chr2:26897790-26897809 MsG0280008185.01.T01:CDS 25.0%
!! GAAAATCATCTAAAGTTTCT+GGG + Chr2:26897178-26897197 None:intergenic 25.0%
!!! AATCAACTAATAACGGATTA+CGG - Chr2:26897797-26897816 MsG0280008185.01.T01:CDS 25.0%
!!! ACAATTTTTCGAGAATACAT+TGG - Chr2:26897140-26897159 MsG0280008185.01.T01:CDS 25.0%
AATCAATTCCACTTCATTCT+AGG - Chr2:26897224-26897243 MsG0280008185.01.T01:CDS 30.0%
ATTCAATCCTAAGGAGAAAA+AGG - Chr2:26897406-26897425 MsG0280008185.01.T01:CDS 30.0%
CAATCAAACTATATCCTCAA+AGG - Chr2:26897754-26897773 MsG0280008185.01.T01:CDS 30.0%
GTATAAAGATGGAAAAGGAA+CGG - Chr2:26897262-26897281 MsG0280008185.01.T01:CDS 30.0%
TATAAAGATGGAAAAGGAAC+GGG - Chr2:26897263-26897282 MsG0280008185.01.T01:CDS 30.0%
! AAAAATTGTTGGCCTCAAAA+CGG + Chr2:26897129-26897148 None:intergenic 30.0%
! GGAAAATCATCTAAAGTTTC+TGG + Chr2:26897179-26897198 None:intergenic 30.0%
! TTTTTCTCCTTAGGATTGAA+TGG + Chr2:26897407-26897426 None:intergenic 30.0%
!! CATAGTTGATATCCATTTTG+TGG + Chr2:26897718-26897737 None:intergenic 30.0%
!!! TGTGTTTGAACACTTTTTCT+GGG + Chr2:26897325-26897344 None:intergenic 30.0%
!!! TTGTGTTTGAACACTTTTTC+TGG + Chr2:26897326-26897345 None:intergenic 30.0%
AAGGTTATGATAACTATCGG+AGG - Chr2:26897365-26897384 MsG0280008185.01.T01:CDS 35.0%
ATCATCAAACTCATCCTTTG+AGG + Chr2:26897771-26897790 None:intergenic 35.0%
ATCCTAAGGAGAAAAAGGTA+TGG - Chr2:26897411-26897430 MsG0280008185.01.T01:CDS 35.0%
CCAAAACAATTTGTGGTAGT+GGG + Chr2:26897693-26897712 None:intergenic 35.0%
GAGGTGTATAAAGATGGAAA+AGG - Chr2:26897257-26897276 MsG0280008185.01.T01:CDS 35.0%
TAAGCATGTATCCAAATCCA+TGG - Chr2:26897631-26897650 MsG0280008185.01.T01:CDS 35.0%
TACCAGAATCATCATTGACA+AGG + Chr2:26897490-26897509 None:intergenic 35.0%
TACCTTGTCAATGATGATTC+TGG - Chr2:26897485-26897504 MsG0280008185.01.T01:CDS 35.0%
TGAATGGATATTTAGTGCCA+TGG + Chr2:26897391-26897410 None:intergenic 35.0%
! AATAACTTGTCCAATGCAGT+TGG + Chr2:26897594-26897613 None:intergenic 35.0%
! ATCCATACCTTTTTCTCCTT+AGG + Chr2:26897416-26897435 None:intergenic 35.0%
! CCACTACCACAAATTGTTTT+GGG - Chr2:26897691-26897710 MsG0280008185.01.T01:CDS 35.0%
! TCATCATTGATGATCTCAAC+AGG + Chr2:26897200-26897219 None:intergenic 35.0%
! TTACGGTGAGCTTAAACTTT+TGG - Chr2:26897814-26897833 MsG0280008185.01.T01:CDS 35.0%
!! ATATCGATTCTAGTGTGACT+GGG - Chr2:26897558-26897577 MsG0280008185.01.T01:CDS 35.0%
!! TATATCGATTCTAGTGTGAC+TGG - Chr2:26897557-26897576 MsG0280008185.01.T01:CDS 35.0%
!!! ACAATATCAATGCCGTTTTG+AGG - Chr2:26897114-26897133 MsG0280008185.01.T01:CDS 35.0%
!!! ATTGTTTTGGGACCACAAAA+TGG - Chr2:26897703-26897722 MsG0280008185.01.T01:CDS 35.0%
AGCAATGGACACATATTCCA+TGG + Chr2:26897651-26897670 None:intergenic 40.0%
AGCATTTCCTCCAAGATGTA+AGG + Chr2:26897989-26898008 None:intergenic 40.0%
ATCAACAGCAAATCACTCCT+TGG - Chr2:26897908-26897927 MsG0280008185.01.T01:CDS 40.0%
CACTCCAAACAAAAAGTCCA+AGG + Chr2:26897928-26897947 None:intergenic 40.0%
CACTGCACTAGCATAATTGA+TGG - Chr2:26897515-26897534 MsG0280008185.01.T01:CDS 40.0%
CATCAATTATGCTAGTGCAG+TGG + Chr2:26897517-26897536 None:intergenic 40.0%
CATCTTAGTATCAGCTGGAT+CGG + Chr2:26897852-26897871 None:intergenic 40.0%
CCCAAAACAATTTGTGGTAG+TGG + Chr2:26897694-26897713 None:intergenic 40.0%
GATATGTCTGATCGAGCTTA+TGG + Chr2:26897447-26897466 None:intergenic 40.0%
GGACACATATTCCATGGATT+TGG + Chr2:26897645-26897664 None:intergenic 40.0%
TCCATCTTTATACACCTCAG+TGG + Chr2:26897255-26897274 None:intergenic 40.0%
TGATACTAAGATGAACCGAG+AGG - Chr2:26897859-26897878 MsG0280008185.01.T01:CDS 40.0%
TGTGGTCCCAAAACAATTTG+TGG + Chr2:26897700-26897719 None:intergenic 40.0%
! CACTCCTTGGACTTTTTGTT+TGG - Chr2:26897921-26897940 MsG0280008185.01.T01:CDS 40.0%
! CCCACTACCACAAATTGTTT+TGG - Chr2:26897690-26897709 MsG0280008185.01.T01:CDS 40.0%
!! TATCGATTCTAGTGTGACTG+GGG - Chr2:26897559-26897578 MsG0280008185.01.T01:CDS 40.0%
ATGATAACTATCGGAGGCCA+TGG - Chr2:26897371-26897390 MsG0280008185.01.T01:CDS 45.0%
CGGTTCATCTTAGTATCAGC+TGG + Chr2:26897857-26897876 None:intergenic 45.0%
GCCACTGAGGTGTATAAAGA+TGG - Chr2:26897251-26897270 MsG0280008185.01.T01:CDS 45.0%
TAAGGCTCGATGTCATTGCT+CGG + Chr2:26897971-26897990 None:intergenic 45.0%
TCGAGAATACATTGGCGTGA+AGG - Chr2:26897148-26897167 MsG0280008185.01.T01:CDS 45.0%
TGACATCGAGCCTTACATCT+TGG - Chr2:26897976-26897995 MsG0280008185.01.T01:CDS 45.0%
! GGTGAGCTTAAACTTTTGGC+AGG - Chr2:26897818-26897837 MsG0280008185.01.T01:CDS 45.0%
!! AACCGAGAGGTGTTCATTGA+GGG - Chr2:26897872-26897891 MsG0280008185.01.T01:CDS 45.0%
!! ACAATTTGTGGTAGTGGGCT+TGG + Chr2:26897688-26897707 None:intergenic 45.0%
CATCGAGCCTTACATCTTGG+AGG - Chr2:26897979-26897998 MsG0280008185.01.T01:CDS 50.0%
TGGCGAAGCCTAGAATGAAG+TGG + Chr2:26897235-26897254 None:intergenic 50.0%
! GCTGACTTCTCCAACTGCAT+TGG - Chr2:26897581-26897600 MsG0280008185.01.T01:CDS 50.0%
!! ATTTGTGGTAGTGGGCTTGG+AGG + Chr2:26897685-26897704 None:intergenic 50.0%
!! GAACCGAGAGGTGTTCATTG+AGG - Chr2:26897871-26897890 MsG0280008185.01.T01:CDS 50.0%
CATTCTAGGCTTCGCCACTG+AGG - Chr2:26897238-26897257 MsG0280008185.01.T01:CDS 55.0%
CGCCCTCAATGAACACCTCT+CGG + Chr2:26897877-26897896 None:intergenic 55.0%
CGGGACATTTCGTTCGCGAT+TGG - Chr2:26897282-26897301 MsG0280008185.01.T01:CDS 55.0%
GCTCGATGTCATTGCTCGGT+GGG + Chr2:26897967-26897986 None:intergenic 55.0%
! GATGTCATTGCTCGGTGGGT+TGG + Chr2:26897963-26897982 None:intergenic 55.0%
GAGGAGCTCTGTCGGAGCAA+TGG + Chr2:26897666-26897685 None:intergenic 60.0%
GGCTCGATGTCATTGCTCGG+TGG + Chr2:26897968-26897987 None:intergenic 60.0%
TGGGCTTGGAGGAGCTCTGT+CGG + Chr2:26897674-26897693 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 26897098 26898033 26897098 ID=MsG0280008185.01;Name=MsG0280008185.01
Chr2 mRNA 26897098 26898033 26897098 ID=MsG0280008185.01.T01;Parent=MsG0280008185.01;Name=MsG0280008185.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|311
Chr2 exon 26897098 26898033 26897098 ID=MsG0280008185.01.T01:exon:14220;Parent=MsG0280008185.01.T01
Chr2 CDS 26897098 26898033 26897098 ID=MsG0280008185.01.T01:cds;Parent=MsG0280008185.01.T01
Gene Sequence

>MsG0280008185.01.T01

ATGTCTGATCACTCTCACAATATCAATGCCGTTTTGAGGCCAACAATTTTTCGAGAATACATTGGCGTGAAGGATGACCCAGAAACTTTAGATGATTTTCCTGTTGAGATCATCAATGATGACGTCAATCAATTCCACTTCATTCTAGGCTTCGCCACTGAGGTGTATAAAGATGGAAAAGGAACGGGACATTTCGTTCGCGATTGGAACTTTGATTACTTCAGCCCAGAAAAAGTGTTCAAACACAAGAAAGAACATAAGAATATGAAGGTTATGATAACTATCGGAGGCCATGGCACTAAATATCCATTCAATCCTAAGGAGAAAAAGGTATGGATTTTCAATGCCATAAGCTCGATCAGACATATCATTCAAGACTATGAGAACTACCTTGTCAATGATGATTCTGGTAGCTGCCACTGCACTAGCATAATTGATGGTATTGATATTAACTATGAGTATATCGATTCTAGTGTGACTGGGGCTGACTTCTCCAACTGCATTGGACAAGTTATTAAACATCTCAAGAAGGATAAGCATGTATCCAAATCCATGGAATATGTGTCCATTGCTCCGACAGAGCTCCTCCAAGCCCACTACCACAAATTGTTTTGGGACCACAAAATGGATATCAACTATGTTGACTACAAGTTCTACAATCAAACTATATCCTCAAAGGATGAGTTTGATGATCTCTACAATCAACTAATAACGGATTACGGTGAGCTTAAACTTTTGGCAGGTGTTAGTACCGATCCAGCTGATACTAAGATGAACCGAGAGGTGTTCATTGAGGGCGTCACAAGACTGATCAACAGCAAATCACTCCTTGGACTTTTTGTTTGGAGTGCTAATGACTCTGCCAACCCACCGAGCAATGACATCGAGCCTTACATCTTGGAGGAAATGCTACAAGATCTCCTTACCAATAATTGA

Protein sequence

>MsG0280008185.01.T01

MSDHSHNINAVLRPTIFREYIGVKDDPETLDDFPVEIINDDVNQFHFILGFATEVYKDGKGTGHFVRDWNFDYFSPEKVFKHKKEHKNMKVMITIGGHGTKYPFNPKEKKVWIFNAISSIRHIIQDYENYLVNDDSGSCHCTSIIDGIDINYEYIDSSVTGADFSNCIGQVIKHLKKDKHVSKSMEYVSIAPTELLQAHYHKLFWDHKMDINYVDYKFYNQTISSKDEFDDLYNQLITDYGELKLLAGVSTDPADTKMNREVFIEGVTRLINSKSLLGLFVWSANDSANPPSNDIEPYILEEMLQDLLTNN*