AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008202.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008202.01.T01 MTR_2g036380 68.513 343 31 3 85 350 57 399 2.14e-147 434
MsG0280008202.01.T01 MTR_2g036380 68.513 343 31 3 85 350 147 489 1.84e-146 434
MsG0280008202.01.T01 MTR_2g036380 97.619 84 2 0 1 84 1 84 1.25e-46 169
MsG0280008202.01.T01 MTR_2g036380 65.595 311 30 3 117 350 27 337 3.09e-122 367
MsG0280008202.01.T01 MTR_2g036380 65.359 306 29 3 122 350 1 306 1.12e-119 360
MsG0280008202.01.T01 MTR_4g127580 40.476 336 122 4 87 344 154 489 2.13e-66 226
MsG0280008202.01.T01 MTR_4g127580 60.976 82 32 0 3 84 8 89 3.04e-24 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008202.01.T01 AT4G30120 40.841 333 119 5 91 345 1 333 1.94e-77 243
MsG0280008202.01.T01 AT4G30120 40.653 337 122 5 87 345 156 492 7.35e-76 243
MsG0280008202.01.T01 AT4G30120 38.217 157 80 3 4 154 11 156 7.04e-22 97.1
MsG0280008202.01.T01 AT4G30110 39.583 336 125 4 88 345 151 486 5.80e-75 249
MsG0280008202.01.T01 AT4G30110 44.538 119 54 2 3 114 4 117 3.56e-20 92.4
MsG0280008202.01.T01 AT4G30110 39.583 336 125 4 88 345 151 486 5.80e-75 249
MsG0280008202.01.T01 AT4G30110 44.538 119 54 2 3 114 4 117 3.56e-20 92.4
MsG0280008202.01.T01 AT2G19110 39.881 336 124 4 88 345 161 496 2.79e-69 235
MsG0280008202.01.T01 AT2G19110 37.073 205 107 6 5 201 16 206 6.87e-23 100
MsG0280008202.01.T01 AT2G19110 39.881 336 124 4 88 345 161 496 2.79e-69 235
MsG0280008202.01.T01 AT2G19110 37.073 205 107 6 5 201 16 206 6.87e-23 100
MsG0280008202.01.T01 AT2G19110 39.881 336 124 4 88 345 161 496 2.79e-69 235
MsG0280008202.01.T01 AT2G19110 37.073 205 107 6 5 201 16 206 6.87e-23 100

Find 58 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATTTCTGAGTCACAAAT+AGG 0.288449 2:+27141826 MsG0280008202.01.T01:CDS
ATTTGAGTATATATCATTTC+AGG 0.320696 2:+27143461 MsG0280008202.01.T01:intron
TGCATTAAGCGTTCCTGATA+TGG 0.332596 2:+27144232 MsG0280008202.01.T01:CDS
TGAAACCATGGCTCCATATC+AGG 0.359100 2:-27144245 None:intergenic
TTTGCCAAGTACTATATTCC+TGG 0.369583 2:+27143599 MsG0280008202.01.T01:CDS
TGAGAACTCTCCTCTTGTTA+TGG 0.378498 2:-27144439 None:intergenic
GTTCAGAACTTAATTCTTGA+AGG 0.379504 2:+27145242 MsG0280008202.01.T01:CDS
CTATTTGTGACTCAGAAATA+AGG 0.391338 2:-27141825 None:intergenic
GAGAAGCAACTTTGAAGTGT+TGG 0.403762 2:+27141684 MsG0280008202.01.T01:CDS
AGAAGCAACTTTGAAGTGTT+GGG 0.415170 2:+27141685 MsG0280008202.01.T01:CDS
AGAATTCTGAAGCCTCTTCA+TGG 0.416439 2:+27141742 MsG0280008202.01.T01:CDS
AGGAGCCATGTTTGTTAAAG+AGG 0.421947 2:-27142959 None:intergenic
GATGGTAGGGATATCTATAT+AGG 0.426846 2:+27145175 MsG0280008202.01.T01:CDS
AACAAACATGGCTCCTCAAA+TGG 0.438346 2:+27142966 MsG0280008202.01.T01:CDS
ATGGCTATTGTTGCTGAGAC+CGG 0.458297 2:+27142985 MsG0280008202.01.T01:CDS
TCCACTAGTGCACCTGCCAC+TGG 0.469705 2:-27145067 None:intergenic
TGGTGATGCTATTCCCTTGA+TGG 0.473105 2:+27143062 MsG0280008202.01.T01:CDS
CTTCCACAACAATTCCATCA+AGG 0.480571 2:-27143076 None:intergenic
TGGCTATTGTTGCTGAGACC+GGG 0.484594 2:+27142986 MsG0280008202.01.T01:CDS
TCAGGAACGCTTAATGCAGC+TGG 0.498871 2:-27144227 None:intergenic
TGAGACAAGAGCAACTCATA+AGG 0.500504 2:+27142576 MsG0280008202.01.T01:CDS
ATGGTTTCATTTAGCACTTG+TGG 0.510070 2:+27144259 MsG0280008202.01.T01:CDS
ACCATCCTTGCAGTCAAGGC+TGG 0.510481 2:+27143042 MsG0280008202.01.T01:CDS
ATTGACATCAACACGTTCCC+CGG 0.514951 2:-27143004 None:intergenic
CATACCAGGAATATAGTACT+TGG 0.516015 2:-27143603 None:intergenic
ACAATGAGACCTTACTGTAC+TGG 0.518718 2:+27144493 MsG0280008202.01.T01:CDS
ACCAGCCTTGACTGCAAGGA+TGG 0.526545 2:-27143043 None:intergenic
AATAGAGTTCGTGTCAGAGC+TGG 0.528801 2:+27145202 MsG0280008202.01.T01:CDS
TCATTATTGGTCTCTCCTTG+TGG 0.530580 2:-27142163 None:intergenic
GTCAGAGCTGGCTGTAAAAG+AGG 0.531547 2:+27145214 MsG0280008202.01.T01:CDS
TCCAGTGGCAGGTGCACTAG+TGG 0.531604 2:+27145066 MsG0280008202.01.T01:CDS
TTCCCTTGATGGAATTGTTG+TGG 0.534625 2:+27143073 MsG0280008202.01.T01:CDS
CAGAAATAAGGAGAACATCA+TGG 0.536453 2:-27141813 None:intergenic
GAACACCATCCTTGCAGTCA+AGG 0.543189 2:+27143038 MsG0280008202.01.T01:CDS
AAATCAAGTCATCCAGTGGC+AGG 0.547267 2:+27145055 MsG0280008202.01.T01:CDS
CATCACCAGCCTTGACTGCA+AGG 0.558354 2:-27143047 None:intergenic
GATTTCAGCTAGCATTGCAG+TGG 0.558685 2:+27144202 MsG0280008202.01.T01:CDS
TGTACCAAAATTCCTTCCCC+CGG 0.558853 2:-27145149 None:intergenic
TTCCACAACAATTCCATCAA+GGG 0.561697 2:-27143075 None:intergenic
TCAAACCAGTCCAGAAAATG+TGG 0.563946 2:+27145110 MsG0280008202.01.T01:CDS
GGCTATTGTTGCTGAGACCG+GGG 0.564489 2:+27142987 MsG0280008202.01.T01:CDS
TTCTGTTCTCTATTGCTCAG+TGG 0.567993 2:+27142551 MsG0280008202.01.T01:CDS
CTTGATGGAATTGTTGTGGA+AGG 0.568254 2:+27143077 MsG0280008202.01.T01:CDS
TCAGCAACAATAGCCATTTG+AGG 0.581659 2:-27142979 None:intergenic
ACAGCTTTGACTCCATGAAG+AGG 0.594671 2:-27141754 None:intergenic
GCGTTCCTGATATGGAGCCA+TGG 0.600241 2:+27144240 MsG0280008202.01.T01:CDS
GAAAACGTACCAGTACAGTA+AGG 0.604281 2:-27144502 None:intergenic
AAAACAGGCACCATAACAAG+AGG 0.622472 2:+27144429 MsG0280008202.01.T01:intron
AAGTGTAAGAACAACTGTGT+TGG 0.622963 2:+27143490 MsG0280008202.01.T01:CDS
GTGCACTAGTGGATTATGCA+AGG 0.630355 2:+27145077 MsG0280008202.01.T01:CDS
GATGTCCTCTTTAACAAACA+TGG 0.640546 2:+27142954 MsG0280008202.01.T01:CDS
TTGTGCAACAGAAGCAACAC+TGG 0.648324 2:+27141714 MsG0280008202.01.T01:CDS
ACAAGTGCTAAATGAAACCA+TGG 0.656317 2:-27144257 None:intergenic
CTGATGCACTGAATACAGCA+AGG 0.658656 2:+27142122 MsG0280008202.01.T01:CDS
ACAACAGTAACTGTTCGAGA+AGG 0.683912 2:-27141790 None:intergenic
GAGTAAATCAAGTCATCCAG+TGG 0.696182 2:+27145051 MsG0280008202.01.T01:CDS
TGTGTTGGCGAAAGACACAA+TGG 0.738132 2:+27143505 MsG0280008202.01.T01:CDS
GTTGGCGAAAGACACAATGG+TGG 0.805085 2:+27143508 MsG0280008202.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATGTTAGTACTTT+AGG + Chr2:27144915-27144934 MsG0280008202.01.T01:intron 15.0%
!! GAAAATGAAAATAGAAAATA+TGG - Chr2:27143236-27143255 None:intergenic 15.0%
!!! ATTTTGTAATACGTAATTAT+TGG - Chr2:27144139-27144158 None:intergenic 15.0%
!!! CTATCTTATTTATTAATCTA+AGG - Chr2:27143710-27143729 None:intergenic 15.0%
!!! TTTGAATATCTAAAATATCT+AGG + Chr2:27141903-27141922 MsG0280008202.01.T01:intron 15.0%
!! AAATCACTATAAGAAAACAT+TGG + Chr2:27142873-27142892 MsG0280008202.01.T01:intron 20.0%
!! AACATGAATTTGTATATCTT+AGG + Chr2:27141874-27141893 MsG0280008202.01.T01:intron 20.0%
!! ACATGAATTTGTATATCTTA+GGG + Chr2:27141875-27141894 MsG0280008202.01.T01:intron 20.0%
!! ATTTGAGTATATATCATTTC+AGG + Chr2:27143461-27143480 MsG0280008202.01.T01:intron 20.0%
!! CTATTGAACAAAATAATGTA+CGG + Chr2:27144531-27144550 MsG0280008202.01.T01:intron 20.0%
!! GAATAGAAAAAAATGTGATT+GGG - Chr2:27143932-27143951 None:intergenic 20.0%
!! TACATTAACGACATAAAATT+TGG - Chr2:27142682-27142701 None:intergenic 20.0%
!! TACATTACCTTATTAATTGT+TGG + Chr2:27143735-27143754 MsG0280008202.01.T01:intron 20.0%
!! TATACAAATTCATGTTGAAT+AGG - Chr2:27141870-27141889 None:intergenic 20.0%
!! TATGATGTTATAATGTAAGA+AGG - Chr2:27142922-27142941 None:intergenic 20.0%
!! TGAATAGAAAAAAATGTGAT+TGG - Chr2:27143933-27143952 None:intergenic 20.0%
!! TTAAACAATTACAGCATAAA+TGG + Chr2:27144960-27144979 MsG0280008202.01.T01:intron 20.0%
!! TTCTTACATTATAACATCAT+AGG + Chr2:27142921-27142940 MsG0280008202.01.T01:intron 20.0%
!!! ATATCTTAAAATGTTGATTC+TGG - Chr2:27143331-27143350 None:intergenic 20.0%
!!! ATTCCCTAAAGTTTTATTTA+GGG + Chr2:27142297-27142316 MsG0280008202.01.T01:intron 20.0%
!!! GAATTTTTCTTATCAAATCA+TGG - Chr2:27144032-27144051 None:intergenic 20.0%
!!! TACACTTAATATCAACATTT+TGG + Chr2:27142342-27142361 MsG0280008202.01.T01:intron 20.0%
!!! TATCAACATTTTGGTTTTAT+TGG + Chr2:27142351-27142370 MsG0280008202.01.T01:intron 20.0%
!!! TTTTAATTTTTTCTGTGTGA+TGG + Chr2:27141941-27141960 MsG0280008202.01.T01:intron 20.0%
! AATTATTGGACACATTAATC+AGG - Chr2:27144125-27144144 None:intergenic 25.0%
! ATAGCCCTAAATAAAACTTT+AGG - Chr2:27142304-27142323 None:intergenic 25.0%
! GAAAATTGTGAGAAAAATGT+TGG - Chr2:27143291-27143310 None:intergenic 25.0%
! GATATACTTACAATGATATG+TGG + Chr2:27142198-27142217 MsG0280008202.01.T01:intron 25.0%
! GTACATCAAATTTCTTATTG+TGG + Chr2:27143632-27143651 MsG0280008202.01.T01:intron 25.0%
! GTGATAGAGATGAAATATAA+GGG - Chr2:27143410-27143429 None:intergenic 25.0%
! GTTAAGTATCTTAGAAAGTT+TGG + Chr2:27143198-27143217 MsG0280008202.01.T01:intron 25.0%
! TAAAGAAAACATGTTTGCAT+TGG + Chr2:27142425-27142444 MsG0280008202.01.T01:intron 25.0%
! TAGCCCTAAATAAAACTTTA+GGG - Chr2:27142303-27142322 None:intergenic 25.0%
! TGTGATAGAGATGAAATATA+AGG - Chr2:27143411-27143430 None:intergenic 25.0%
! TTTCAGACATTTGTAGAAAA+TGG - Chr2:27142383-27142402 None:intergenic 25.0%
!! CTGACATTTCTTTTCATTAT+TGG - Chr2:27142179-27142198 None:intergenic 25.0%
!! GTTGATGAAAAAATGTTAAC+AGG + Chr2:27143110-27143129 MsG0280008202.01.T01:intron 25.0%
!! TCATAACTTACTTTGTTTCT+TGG + Chr2:27141965-27141984 MsG0280008202.01.T01:intron 25.0%
!! TGGAGAATTTTCAAAATTTC+CGG + Chr2:27145130-27145149 MsG0280008202.01.T01:CDS 25.0%
!!! AACACTTTCTAGAATTAAGA+CGG + Chr2:27144396-27144415 MsG0280008202.01.T01:intron 25.0%
!!! ATAAGTGGATTACTATTGAA+AGG + Chr2:27144358-27144377 MsG0280008202.01.T01:intron 25.0%
!!! CACATTTTTTTCTATTCACA+AGG + Chr2:27143935-27143954 MsG0280008202.01.T01:intron 25.0%
!!! GATTCCCTAAAGTTTTATTT+AGG + Chr2:27142296-27142315 MsG0280008202.01.T01:intron 25.0%
!!! GTTTTTGGTAATCATGTTAT+TGG + Chr2:27143825-27143844 MsG0280008202.01.T01:intron 25.0%
AACAAAATAATGTACGGTAG+GGG + Chr2:27144537-27144556 MsG0280008202.01.T01:intron 30.0%
ACACTAATACTGACATTAAG+GGG - Chr2:27143767-27143786 None:intergenic 30.0%
AGAAAAAAATGTGATTGGGA+GGG - Chr2:27143928-27143947 None:intergenic 30.0%
AGAAAGAAAACATAGTGACA+AGG - Chr2:27142628-27142647 None:intergenic 30.0%
ATGAAACTCAAAGGAATTGA+TGG + Chr2:27144782-27144801 MsG0280008202.01.T01:intron 30.0%
CAAATTTCTTATTGTGGAAG+TGG + Chr2:27143638-27143657 MsG0280008202.01.T01:intron 30.0%
CACACTAATACTGACATTAA+GGG - Chr2:27143768-27143787 None:intergenic 30.0%
CATTATAACATCATAGGCAA+TGG + Chr2:27142927-27142946 MsG0280008202.01.T01:intron 30.0%
CCTAATGTAATGTGGAAATT+AGG + Chr2:27144005-27144024 MsG0280008202.01.T01:intron 30.0%
CCTAATTTCCACATTACATT+AGG - Chr2:27144008-27144027 None:intergenic 30.0%
CTATTTGTGACTCAGAAATA+AGG - Chr2:27141828-27141847 None:intergenic 30.0%
GAACAAAATAATGTACGGTA+GGG + Chr2:27144536-27144555 MsG0280008202.01.T01:intron 30.0%
GCTGGAACTATTAATATGAA+TGG + Chr2:27143176-27143195 MsG0280008202.01.T01:intron 30.0%
GGACTTTCCAACAATTAATA+AGG - Chr2:27143745-27143764 None:intergenic 30.0%
GTGCTTATGTATAAGCTATT+TGG + Chr2:27144994-27145013 MsG0280008202.01.T01:intron 30.0%
TAGAAAAAAATGTGATTGGG+AGG - Chr2:27143929-27143948 None:intergenic 30.0%
TGAAACTCAAAGGAATTGAT+GGG + Chr2:27144783-27144802 MsG0280008202.01.T01:intron 30.0%
TGAACAAAATAATGTACGGT+AGG + Chr2:27144535-27144554 MsG0280008202.01.T01:intron 30.0%
TGAACCAAAACCGTATTTAA+GGG + Chr2:27143960-27143979 MsG0280008202.01.T01:intron 30.0%
TGAAGTATATATCCTCCTTT+GGG + Chr2:27142244-27142263 MsG0280008202.01.T01:intron 30.0%
TGGTATTAATCGAGTAATGA+AGG - Chr2:27142662-27142681 None:intergenic 30.0%
TTGAACCAAAACCGTATTTA+AGG + Chr2:27143959-27143978 MsG0280008202.01.T01:intron 30.0%
TTGAAGTATATATCCTCCTT+TGG + Chr2:27142243-27142262 MsG0280008202.01.T01:intron 30.0%
! AGAATTTTCAAAATTTCCGG+GGG + Chr2:27145133-27145152 MsG0280008202.01.T01:CDS 30.0%
! ATGAGTTTGTTTATTCAAGC+AGG - Chr2:27144941-27144960 None:intergenic 30.0%
! ATTAAGTGTAAGAGCTCTAA+TGG - Chr2:27142332-27142351 None:intergenic 30.0%
! GAGAATTTTCAAAATTTCCG+GGG + Chr2:27145132-27145151 MsG0280008202.01.T01:CDS 30.0%
! GCTTTACAAGATTTTACAGA+TGG + Chr2:27142514-27142533 MsG0280008202.01.T01:CDS 30.0%
! GGAGAATTTTCAAAATTTCC+GGG + Chr2:27145131-27145150 MsG0280008202.01.T01:CDS 30.0%
! TAAAACTTTAGGGAATCCAA+TGG - Chr2:27142293-27142312 None:intergenic 30.0%
! TGAAAATTCTCCACATTTTC+TGG - Chr2:27145123-27145142 None:intergenic 30.0%
! TGGTTAACGAAAACTTTTGT+AGG + Chr2:27145014-27145033 MsG0280008202.01.T01:intron 30.0%
! TTACAAGATTTTACAGATGG+TGG + Chr2:27142517-27142536 MsG0280008202.01.T01:CDS 30.0%
!! AGGAATTTTGGTACAATTGA+TGG + Chr2:27145157-27145176 MsG0280008202.01.T01:CDS 30.0%
!! ATTTTGGTACAATTGATGGT+AGG + Chr2:27145161-27145180 MsG0280008202.01.T01:CDS 30.0%
!! CTTATTTCTGAGTCACAAAT+AGG + Chr2:27141826-27141845 MsG0280008202.01.T01:CDS 30.0%
!! TTTTGGTACAATTGATGGTA+GGG + Chr2:27145162-27145181 MsG0280008202.01.T01:CDS 30.0%
!!! AACATTTTGGTTTTATTGGC+AGG + Chr2:27142355-27142374 MsG0280008202.01.T01:intron 30.0%
!!! TCAGAACAATGCTTTGTTTT+TGG + Chr2:27143810-27143829 MsG0280008202.01.T01:intron 30.0%
AAACTCAAAGGAATTGATGG+GGG + Chr2:27144785-27144804 MsG0280008202.01.T01:intron 35.0%
AAACTCGGATCCCTTAAATA+CGG - Chr2:27143973-27143992 None:intergenic 35.0%
AAGAATCAGATTCACTTGTC+TGG + Chr2:27143153-27143172 MsG0280008202.01.T01:intron 35.0%
AAGTGTAAGAACAACTGTGT+TGG + Chr2:27143490-27143509 MsG0280008202.01.T01:CDS 35.0%
ACAAGTGCTAAATGAAACCA+TGG - Chr2:27144260-27144279 None:intergenic 35.0%
AGAATCAGATTCACTTGTCT+GGG + Chr2:27143154-27143173 MsG0280008202.01.T01:intron 35.0%
ATGGTTTCATTTAGCACTTG+TGG + Chr2:27144259-27144278 MsG0280008202.01.T01:CDS 35.0%
CACTAATACTGACATTAAGG+GGG - Chr2:27143766-27143785 None:intergenic 35.0%
CAGAAATAAGGAGAACATCA+TGG - Chr2:27141816-27141835 None:intergenic 35.0%
CATACCAGGAATATAGTACT+TGG - Chr2:27143606-27143625 None:intergenic 35.0%
CATTAATCAGGCTCAGTATT+TGG - Chr2:27144113-27144132 None:intergenic 35.0%
CCACACTAATACTGACATTA+AGG - Chr2:27143769-27143788 None:intergenic 35.0%
CCTTAATGTCAGTATTAGTG+TGG + Chr2:27143766-27143785 MsG0280008202.01.T01:intron 35.0%
CTGTTTATGTTGTATTGCAG+TGG + Chr2:27144172-27144191 MsG0280008202.01.T01:intron 35.0%
GAAAAAAATGTGATTGGGAG+GGG - Chr2:27143927-27143946 None:intergenic 35.0%
GAAACTCAAAGGAATTGATG+GGG + Chr2:27144784-27144803 MsG0280008202.01.T01:intron 35.0%
GACAAGTGAATCTGATTCTT+TGG - Chr2:27143153-27143172 None:intergenic 35.0%
GATCCGAGTTTAATTCCATT+GGG + Chr2:27143982-27144001 MsG0280008202.01.T01:intron 35.0%
GATGGTAGGGATATCTATAT+AGG + Chr2:27145175-27145194 MsG0280008202.01.T01:CDS 35.0%
GATGTCCTCTTTAACAAACA+TGG + Chr2:27142954-27142973 MsG0280008202.01.T01:CDS 35.0%
GTGTGCATATTAATGTACTC+CGG + Chr2:27142798-27142817 MsG0280008202.01.T01:intron 35.0%
TACTCTTGCTTCTTGCTTAA+GGG + Chr2:27142750-27142769 MsG0280008202.01.T01:intron 35.0%
TCATCATATGTTGCAGTATG+TGG + Chr2:27142484-27142503 MsG0280008202.01.T01:intron 35.0%
TTCCACAACAATTCCATCAA+GGG - Chr2:27143078-27143097 None:intergenic 35.0%
TTCGCAAGAATGAAACTCAA+AGG + Chr2:27144773-27144792 MsG0280008202.01.T01:intron 35.0%
TTTGCCAAGTACTATATTCC+TGG + Chr2:27143599-27143618 MsG0280008202.01.T01:CDS 35.0%
! AATCTGATTCTTTGGTGACA+GGG - Chr2:27143145-27143164 None:intergenic 35.0%
! AGATTAGTCATCGACAAAGT+CGG - Chr2:27143864-27143883 None:intergenic 35.0%
! TTGTTTCTTGGATTCATGTG+TGG + Chr2:27141977-27141996 MsG0280008202.01.T01:intron 35.0%
!! ACTTTCTAGAATTAAGACGG+TGG + Chr2:27144399-27144418 MsG0280008202.01.T01:intron 35.0%
!! AGAAGCAACTTTGAAGTGTT+GGG + Chr2:27141685-27141704 MsG0280008202.01.T01:CDS 35.0%
!! AGTGGATTACTATTGAAAGG+TGG + Chr2:27144361-27144380 MsG0280008202.01.T01:intron 35.0%
!! TCAGTAACAGATTTTAGTGC+TGG + Chr2:27144459-27144478 MsG0280008202.01.T01:CDS 35.0%
AAAACAGGCACCATAACAAG+AGG + Chr2:27144429-27144448 MsG0280008202.01.T01:intron 40.0%
AACAAACATGGCTCCTCAAA+TGG + Chr2:27142966-27142985 MsG0280008202.01.T01:CDS 40.0%
AACTCAAAGGAATTGATGGG+GGG + Chr2:27144786-27144805 MsG0280008202.01.T01:intron 40.0%
ACAAAAGATTGCAACTGGTG+TGG - Chr2:27144320-27144339 None:intergenic 40.0%
ACAACAGTAACTGTTCGAGA+AGG - Chr2:27141793-27141812 None:intergenic 40.0%
ACAATGAGACCTTACTGTAC+TGG + Chr2:27144493-27144512 MsG0280008202.01.T01:CDS 40.0%
ACATAGTGACAAGGAGAAAG+AGG - Chr2:27142619-27142638 None:intergenic 40.0%
AGAGCACAAAAGATTGCAAC+TGG - Chr2:27144325-27144344 None:intergenic 40.0%
AGGCCCAATGGAATTAAACT+CGG - Chr2:27143988-27144007 None:intergenic 40.0%
ATGTCAGTATTAGTGTGGTG+AGG + Chr2:27143771-27143790 MsG0280008202.01.T01:intron 40.0%
CATAGTGACAAGGAGAAAGA+GGG - Chr2:27142618-27142637 None:intergenic 40.0%
CTATAGCTAGTCACCTTTAG+TGG + Chr2:27143896-27143915 MsG0280008202.01.T01:intron 40.0%
CTCACAAAAGCTGCAATAAG+TGG + Chr2:27144343-27144362 MsG0280008202.01.T01:intron 40.0%
CTTCCACAACAATTCCATCA+AGG - Chr2:27143079-27143098 None:intergenic 40.0%
CTTGATGGAATTGTTGTGGA+AGG + Chr2:27143077-27143096 MsG0280008202.01.T01:CDS 40.0%
GAAAACGTACCAGTACAGTA+AGG - Chr2:27144505-27144524 None:intergenic 40.0%
GAGTAAATCAAGTCATCCAG+TGG + Chr2:27145051-27145070 MsG0280008202.01.T01:CDS 40.0%
GGATCCGAGTTTAATTCCAT+TGG + Chr2:27143981-27144000 MsG0280008202.01.T01:intron 40.0%
GTACTCTTGCTTCTTGCTTA+AGG + Chr2:27142749-27142768 MsG0280008202.01.T01:intron 40.0%
TCAAACCAGTCCAGAAAATG+TGG + Chr2:27145110-27145129 MsG0280008202.01.T01:CDS 40.0%
TCAGCAACAATAGCCATTTG+AGG - Chr2:27142982-27143001 None:intergenic 40.0%
TGAGAACTCTCCTCTTGTTA+TGG - Chr2:27144442-27144461 None:intergenic 40.0%
TGAGACAAGAGCAACTCATA+AGG + Chr2:27142576-27142595 MsG0280008202.01.T01:CDS 40.0%
TGATGTACATGACACATACC+AGG - Chr2:27143620-27143639 None:intergenic 40.0%
TGCATTAAGCGTTCCTGATA+TGG + Chr2:27144232-27144251 MsG0280008202.01.T01:CDS 40.0%
TTCCCTTGATGGAATTGTTG+TGG + Chr2:27143073-27143092 MsG0280008202.01.T01:CDS 40.0%
TTCTGTTCTCTATTGCTCAG+TGG + Chr2:27142551-27142570 MsG0280008202.01.T01:CDS 40.0%
! AGGAGCCATGTTTGTTAAAG+AGG - Chr2:27142962-27142981 None:intergenic 40.0%
! ATTCTCCACATTTTCTGGAC+TGG - Chr2:27145118-27145137 None:intergenic 40.0%
! CACTAAAGGTGACTAGCTAT+AGG - Chr2:27143898-27143917 None:intergenic 40.0%
! GAATCTGATTCTTTGGTGAC+AGG - Chr2:27143146-27143165 None:intergenic 40.0%
! GGATCCCTTAAATACGGTTT+TGG - Chr2:27143967-27143986 None:intergenic 40.0%
! GTATATATCCTCCTTTGGGA+TGG + Chr2:27142248-27142267 MsG0280008202.01.T01:intron 40.0%
! TCATTATTGGTCTCTCCTTG+TGG - Chr2:27142166-27142185 None:intergenic 40.0%
! TTTTCAAAATTTCCGGGGGA+AGG + Chr2:27145137-27145156 MsG0280008202.01.T01:CDS 40.0%
!! AGAATTCTGAAGCCTCTTCA+TGG + Chr2:27141742-27141761 MsG0280008202.01.T01:CDS 40.0%
!! CAAAGCATTGTTCTGAATCG+AGG - Chr2:27143806-27143825 None:intergenic 40.0%
!! GAGAAGCAACTTTGAAGTGT+TGG + Chr2:27141684-27141703 MsG0280008202.01.T01:CDS 40.0%
!!! GAAGCTAGTTTTAGACCACA+AGG + Chr2:27142148-27142167 MsG0280008202.01.T01:CDS 40.0%
AAATCAAGTCATCCAGTGGC+AGG + Chr2:27145055-27145074 MsG0280008202.01.T01:CDS 45.0%
AATAGAGTTCGTGTCAGAGC+TGG + Chr2:27145202-27145221 MsG0280008202.01.T01:CDS 45.0%
AGATTGCAACTGGTGTGGAA+AGG - Chr2:27144315-27144334 None:intergenic 45.0%
ATCGAACTCGAGACCTTCAA+AGG - Chr2:27144851-27144870 None:intergenic 45.0%
ATGGCTATTGTTGCTGAGAC+CGG + Chr2:27142985-27143004 MsG0280008202.01.T01:CDS 45.0%
ATTGACATCAACACGTTCCC+CGG - Chr2:27143007-27143026 None:intergenic 45.0%
CCACATTACATTAGGCCCAA+TGG - Chr2:27144000-27144019 None:intergenic 45.0%
CCATTGGGCCTAATGTAATG+TGG + Chr2:27143997-27144016 MsG0280008202.01.T01:intron 45.0%
CTGATGCACTGAATACAGCA+AGG + Chr2:27142122-27142141 MsG0280008202.01.T01:CDS 45.0%
GATTTCAGCTAGCATTGCAG+TGG + Chr2:27144202-27144221 MsG0280008202.01.T01:CDS 45.0%
GTGGAAAGGATAGAGCACAA+GGG - Chr2:27144301-27144320 None:intergenic 45.0%
TACTCCGGCTTCTTGCTTTA+GGG + Chr2:27142813-27142832 MsG0280008202.01.T01:intron 45.0%
TAGCCGTAACGATGGATACT+AGG + Chr2:27144684-27144703 MsG0280008202.01.T01:intron 45.0%
TAGTATCCATCGTTACGGCT+AGG - Chr2:27144685-27144704 None:intergenic 45.0%
TGAAACCATGGCTCCATATC+AGG - Chr2:27144248-27144267 None:intergenic 45.0%
TGTACCAAAATTCCTTCCCC+CGG - Chr2:27145152-27145171 None:intergenic 45.0%
TGTCGATGACTAATCTCCAC+CGG + Chr2:27143868-27143887 MsG0280008202.01.T01:intron 45.0%
TGTGGAAAGGATAGAGCACA+AGG - Chr2:27144302-27144321 None:intergenic 45.0%
TTGTGCAACAGAAGCAACAC+TGG + Chr2:27141714-27141733 MsG0280008202.01.T01:CDS 45.0%
TTTCCCCTAAAGCAAGAAGC+CGG - Chr2:27142820-27142839 None:intergenic 45.0%
! ACAGCTTTGACTCCATGAAG+AGG - Chr2:27141757-27141776 None:intergenic 45.0%
! ATTTCCGGGGGAAGGAATTT+TGG + Chr2:27145145-27145164 MsG0280008202.01.T01:CDS 45.0%
! GTGACTAGCTATAGGTTCTC+CGG - Chr2:27143890-27143909 None:intergenic 45.0%
! GTTCTGAATCGAGGACATGT+CGG - Chr2:27143797-27143816 None:intergenic 45.0%
! TGGTGATGCTATTCCCTTGA+TGG + Chr2:27143062-27143081 MsG0280008202.01.T01:CDS 45.0%
! TGTGTTGGCGAAAGACACAA+TGG + Chr2:27143505-27143524 MsG0280008202.01.T01:CDS 45.0%
!! AACGGTGAAAGCACTATGCT+GGG + Chr2:27144613-27144632 MsG0280008202.01.T01:intron 45.0%
!! ATATCCTCCTTTGGGATGGT+TGG + Chr2:27142252-27142271 MsG0280008202.01.T01:intron 45.0%
!! GACGGTGGCTTTGATAAAAC+AGG + Chr2:27144414-27144433 MsG0280008202.01.T01:intron 45.0%
!! GCACTTGTGGTTTTACTGAG+TGG + Chr2:27144272-27144291 MsG0280008202.01.T01:intron 45.0%
!! GTGCACTAGTGGATTATGCA+AGG + Chr2:27145077-27145096 MsG0280008202.01.T01:CDS 45.0%
ACTAGCTATAGGTTCTCCGG+TGG - Chr2:27143887-27143906 None:intergenic 50.0%
ACTCCGGCTTCTTGCTTTAG+GGG + Chr2:27142814-27142833 MsG0280008202.01.T01:intron 50.0%
AGTAGTCCTAGCCGTAACGA+TGG + Chr2:27144676-27144695 MsG0280008202.01.T01:intron 50.0%
ATAATGTACGGTAGGGGCAG+AGG + Chr2:27144543-27144562 MsG0280008202.01.T01:intron 50.0%
CAAAGGAATTGATGGGGGGT+TGG + Chr2:27144790-27144809 MsG0280008202.01.T01:intron 50.0%
CAGCATAGTGCTTTCACCGT+TGG - Chr2:27144614-27144633 None:intergenic 50.0%
CCGCCTAGTATCCATCGTTA+CGG - Chr2:27144690-27144709 None:intergenic 50.0%
CCGTAACGATGGATACTAGG+CGG + Chr2:27144687-27144706 MsG0280008202.01.T01:intron 50.0%
CGTTACGGCTAGGACTACTA+GGG - Chr2:27144675-27144694 None:intergenic 50.0%
CTAAACTAGCCCACCGAAGT+TGG - Chr2:27144885-27144904 None:intergenic 50.0%
CTTGGAGTGTGCTCCTTTGA+AGG + Chr2:27144835-27144854 MsG0280008202.01.T01:intron 50.0%
GAACACCATCCTTGCAGTCA+AGG + Chr2:27143038-27143057 MsG0280008202.01.T01:CDS 50.0%
GAGATCGGAAGGAACACCAA+CGG + Chr2:27144595-27144614 MsG0280008202.01.T01:intron 50.0%
GCGATGAAATGCGTGGAGAT+CGG + Chr2:27144580-27144599 MsG0280008202.01.T01:intron 50.0%
GTACTCCGGCTTCTTGCTTT+AGG + Chr2:27142812-27142831 MsG0280008202.01.T01:intron 50.0%
GTCAGAGCTGGCTGTAAAAG+AGG + Chr2:27145214-27145233 MsG0280008202.01.T01:CDS 50.0%
GTTGGCGAAAGACACAATGG+TGG + Chr2:27143508-27143527 MsG0280008202.01.T01:CDS 50.0%
TAATGTACGGTAGGGGCAGA+GGG + Chr2:27144544-27144563 MsG0280008202.01.T01:intron 50.0%
TCAGATTCACTTGTCTGGGC+TGG + Chr2:27143158-27143177 MsG0280008202.01.T01:intron 50.0%
TCAGGAACGCTTAATGCAGC+TGG - Chr2:27144230-27144249 None:intergenic 50.0%
TCGTTACGGCTAGGACTACT+AGG - Chr2:27144676-27144695 None:intergenic 50.0%
TGAAATGCGTGGAGATCGGA+AGG + Chr2:27144584-27144603 MsG0280008202.01.T01:intron 50.0%
TGGCTATTGTTGCTGAGACC+GGG + Chr2:27142986-27143005 MsG0280008202.01.T01:CDS 50.0%
TTCTTGCGAACGTACTCCCT+AGG - Chr2:27144763-27144782 None:intergenic 50.0%
! ATTCTCCCTGGTGCCAACTT+CGG + Chr2:27144869-27144888 MsG0280008202.01.T01:intron 50.0%
!! CAACGGTGAAAGCACTATGC+TGG + Chr2:27144612-27144631 MsG0280008202.01.T01:intron 50.0%
!! GCTCTTGGTTCTGTTGCCAT+TGG + Chr2:27142274-27142293 MsG0280008202.01.T01:intron 50.0%
!! GTGGTGTTGGCTTGAGATCT+TGG + Chr2:27144817-27144836 MsG0280008202.01.T01:intron 50.0%
ACCAGCCTTGACTGCAAGGA+TGG - Chr2:27143046-27143065 None:intergenic 55.0%
ACCATCCTTGCAGTCAAGGC+TGG + Chr2:27143042-27143061 MsG0280008202.01.T01:CDS 55.0%
ACGCGTTAAGTATCCCGCCT+AGG + Chr2:27144743-27144762 MsG0280008202.01.T01:intron 55.0%
AGAGCCAACCATCCCAAAGG+AGG - Chr2:27142259-27142278 None:intergenic 55.0%
CACGCATTTCATCGCTCCAC+CGG - Chr2:27144575-27144594 None:intergenic 55.0%
CATCACCAGCCTTGACTGCA+AGG - Chr2:27143050-27143069 None:intergenic 55.0%
CCAAGAGCCAACCATCCCAA+AGG - Chr2:27142262-27142281 None:intergenic 55.0%
CGCGTTAAGTATCCCGCCTA+GGG + Chr2:27144744-27144763 MsG0280008202.01.T01:intron 55.0%
GATCTCAAGCCAACACCACC+AGG - Chr2:27144816-27144835 None:intergenic 55.0%
GCGTTCCTGATATGGAGCCA+TGG + Chr2:27144240-27144259 MsG0280008202.01.T01:CDS 55.0%
GGCTATTGTTGCTGAGACCG+GGG + Chr2:27142987-27143006 MsG0280008202.01.T01:CDS 55.0%
GGGAGGGGAGATTCCACTAA+AGG - Chr2:27143912-27143931 None:intergenic 55.0%
GTAGGGGCAGAGGGAATTTC+CGG + Chr2:27144553-27144572 MsG0280008202.01.T01:intron 55.0%
GTCTCGAGTTCGATTCTCCC+TGG + Chr2:27144857-27144876 MsG0280008202.01.T01:intron 55.0%
TTGCGAACGTACTCCCTAGG+CGG - Chr2:27144760-27144779 None:intergenic 55.0%
!! CCTTTGGGATGGTTGGCTCT+TGG + Chr2:27142259-27142278 MsG0280008202.01.T01:intron 55.0%
CGGTGGAGCGATGAAATGCG+TGG + Chr2:27144573-27144592 MsG0280008202.01.T01:intron 60.0%
TCCACTAGTGCACCTGCCAC+TGG - Chr2:27145070-27145089 None:intergenic 60.0%
TCCAGTGGCAGGTGCACTAG+TGG + Chr2:27145066-27145085 MsG0280008202.01.T01:CDS 60.0%
TGCGAACGTACTCCCTAGGC+GGG - Chr2:27144759-27144778 None:intergenic 60.0%
! TCCCTGGTGCCAACTTCGGT+GGG + Chr2:27144873-27144892 MsG0280008202.01.T01:intron 60.0%
!! GAATTGATGGGGGGTTGGCC+TGG + Chr2:27144795-27144814 MsG0280008202.01.T01:intron 60.0%
AGCCCACCGAAGTTGGCACC+AGG - Chr2:27144878-27144897 None:intergenic 65.0%
GCCCACCGAAGTTGGCACCA+GGG - Chr2:27144877-27144896 None:intergenic 65.0%
GGGGCAGAGGGAATTTCCGG+TGG + Chr2:27144556-27144575 MsG0280008202.01.T01:intron 65.0%
! CTCCCTGGTGCCAACTTCGG+TGG + Chr2:27144872-27144891 MsG0280008202.01.T01:intron 65.0%
!! TTGATGGGGGGTTGGCCTGG+TGG + Chr2:27144798-27144817 MsG0280008202.01.T01:intron 65.0%
!! GGGGGTTGGCCTGGTGGTGT+TGG + Chr2:27144804-27144823 MsG0280008202.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 27141667 27145261 27141667 ID=MsG0280008202.01;Name=MsG0280008202.01
Chr2 mRNA 27141667 27145261 27141667 ID=MsG0280008202.01.T01;Parent=MsG0280008202.01;Name=MsG0280008202.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|0.71|0.75|8|0|355
Chr2 exon 27141667 27141847 27141667 ID=MsG0280008202.01.T01:exon:14240;Parent=MsG0280008202.01.T01
Chr2 exon 27142122 27142193 27142122 ID=MsG0280008202.01.T01:exon:14241;Parent=MsG0280008202.01.T01
Chr2 exon 27142500 27142597 27142500 ID=MsG0280008202.01.T01:exon:14242;Parent=MsG0280008202.01.T01
Chr2 exon 27142943 27143102 27142943 ID=MsG0280008202.01.T01:exon:14243;Parent=MsG0280008202.01.T01
Chr2 exon 27143468 27143620 27143468 ID=MsG0280008202.01.T01:exon:14244;Parent=MsG0280008202.01.T01
Chr2 exon 27144192 27144290 27144192 ID=MsG0280008202.01.T01:exon:14245;Parent=MsG0280008202.01.T01
Chr2 exon 27144436 27144514 27144436 ID=MsG0280008202.01.T01:exon:14246;Parent=MsG0280008202.01.T01
Chr2 exon 27145036 27145261 27145036 ID=MsG0280008202.01.T01:exon:14247;Parent=MsG0280008202.01.T01
Chr2 CDS 27141667 27141847 27141667 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27142122 27142193 27142122 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27142500 27142597 27142500 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27142943 27143102 27142943 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27143468 27143620 27143468 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27144192 27144290 27144192 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27144436 27144514 27144436 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Chr2 CDS 27145036 27145261 27145036 ID=MsG0280008202.01.T01:cds;Parent=MsG0280008202.01.T01
Gene Sequence

>MsG0280008202.01.T01

ATGGTTGAAAATATAAAGAGAAGCAACTTTGAAGTGTTGGGAATGTGTTGTGCAACAGAAGCAACACTGGTTGAAAGAATTCTGAAGCCTCTTCATGGAGTCAAAGCTGTCTCTGTGATTGTTCCTTCTCGAACAGTTACTGTTGTCCATGATGTTCTCCTTATTTCTGAGTCACAAATAGCTGATGCACTGAATACAGCAAGGCTTGAAGCTAGTTTTAGACCACAAGGAGAGACCAATAATGAAAAGAAATTATGTGGTACTGCTGCTTTACAAGATTTTACAGATGGTGGAGTCATCATCTTTCTGTTCTCTATTGCTCAGTGGCTTGAGACAAGAGCAACTCATAAGGCAATGGTTGCGATGTCCTCTTTAACAAACATGGCTCCTCAAATGGCTATTGTTGCTGAGACCGGGGAACGTGTTGATGTCAATGATGTTAAGATGAACACCATCCTTGCAGTCAAGGCTGGTGATGCTATTCCCTTGATGGAATTGTTGTGGAAGGAAATATATATCATTTCAGGATATATAAGTGTAAGAACAACTGTGTTGGCGAAAGACACAATGGTGGCAAGAATGTCAAGACTTGTTGAAGAAGCTTCTAGCAGAAAATCACAAGCACAAAGATTCATAGATAATTTTGCCAAGTACTATATTCCTGTGGTTGTGTTGATTTCAGCTAGCATTGCAGTGGTTCCAGCTGCATTAAGCGTTCCTGATATGGAGCCATGGTTTCATTTAGCACTTGTGGTTTTACTGAGCACCATAACAAGAGGAGAGTTCTCAGTAACAGATTTTAGTGCTGGAGATGACATCAACAATGAGACCTTACTGTACTGGATTTCGAGTATTGAGAGTAAATCAAGTCATCCAGTGGCAGGTGCACTAGTGGATTATGCAAGGTTGCACTCTATCAAACCAGTCCAGAAAATGTGGAGAATTTTCAAAATTTCCGGGGGAAGGAATTTTGGTACAATTGATGGTAGGGATATCTATATAGGAAACAATAGAGTTCGTGTCAGAGCTGGCTGTAAAAGAGGTGATTGTTCAGAACTTAATTCTTGA
Protein sequence

>MsG0280008202.01.T01

MVENIKRSNFEVLGMCCATEATLVERILKPLHGVKAVSVIVPSRTVTVVHDVLLISESQIADALNTARLEASFRPQGETNNEKKLCGTAALQDFTDGGVIIFLFSIAQWLETRATHKAMVAMSSLTNMAPQMAIVAETGERVDVNDVKMNTILAVKAGDAIPLMELLWKEIYIISGYISVRTTVLAKDTMVARMSRLVEEASSRKSQAQRFIDNFAKYYIPVVVLISASIAVVPAALSVPDMEPWFHLALVVLLSTITRGEFSVTDFSAGDDINNETLLYWISSIESKSSHPVAGALVDYARLHSIKPVQKMWRIFKISGGRNFGTIDGRDIYIGNNRVRVRAGCKRGDCSELNS*