Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009623.01.T01 | XP_013464289.1 | 88.55 | 131 | 13 | 1 | 1 | 131 | 358 | 486 | 2.11E-71 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009623.01.T01 | Q7FAZ2 | 40.164 | 122 | 65 | 1 | 1 | 122 | 132 | 245 | 5.72E-23 | 96.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009623.01.T01 | A0A072V8H5 | 88.550 | 131 | 13 | 1 | 1 | 131 | 358 | 486 | 1.01e-71 | 241 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000977.01 | MsG0280009623.01 | 0.811211 | 7.843016e-51 | 4.825919e-48 |
| MsG0180001590.01 | MsG0280009623.01 | 0.809663 | 1.693209e-50 | 1.000129e-47 |
| MsG0280006918.01 | MsG0280009623.01 | 0.805986 | 1.024032e-49 | 5.494765e-47 |
| MsG0280006919.01 | MsG0280009623.01 | 0.810162 | 1.322208e-50 | 7.912323e-48 |
| MsG0280009623.01 | MsG0280009624.01 | 0.818151 | 2.275903e-52 | 1.690165e-49 |
| MsG0280009623.01 | MsG0280011135.01 | 0.820989 | 5.124986e-53 | 4.118163e-50 |
| MsG0280009623.01 | MsG0480022535.01 | 0.821298 | 4.350045e-53 | 3.525133e-50 |
| MsG0280009623.01 | MsG0480022572.01 | 0.807702 | 4.443769e-50 | 2.492090e-47 |
| MsG0280009623.01 | MsG0580024340.01 | 0.805596 | 1.236650e-49 | 6.569036e-47 |
| MsG0280009623.01 | MsG0580029182.01 | 0.801076 | 1.067721e-48 | 5.052953e-46 |
| MsG0280009623.01 | MsG0580029346.01 | 0.830445 | 2.925542e-55 | 3.082764e-52 |
| MsG0280009623.01 | MsG0780036050.01 | 0.807036 | 6.150430e-50 | 3.390062e-47 |
| MsG0280009623.01 | MsG0780039438.01 | 0.824223 | 9.065516e-54 | 7.980767e-51 |
| MsG0280009623.01 | MsG0780039964.01 | 0.816242 | 6.118713e-52 | 4.312491e-49 |
| MsG0280009623.01 | MsG0880044612.01 | 0.800955 | 1.130433e-48 | 5.333330e-46 |
| MsG0280009623.01 | MsG0880044615.01 | 0.817721 | 2.847025e-52 | 2.089532e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009623.01.T01 | MTR_2g067980 | 88.550 | 131 | 13 | 1 | 1 | 131 | 358 | 486 | 2.56e-75 | 241 |
| MsG0280009623.01.T01 | MTR_2g067990 | 77.863 | 131 | 27 | 1 | 1 | 131 | 335 | 463 | 9.71e-63 | 207 |
| MsG0280009623.01.T01 | MTR_2g068650 | 73.171 | 123 | 25 | 2 | 9 | 131 | 90 | 204 | 3.96e-51 | 173 |
| MsG0280009623.01.T01 | MTR_2g067970 | 53.333 | 135 | 54 | 6 | 1 | 128 | 295 | 427 | 2.34e-31 | 117 |
| MsG0280009623.01.T01 | MTR_8g011430 | 43.333 | 120 | 65 | 1 | 1 | 120 | 133 | 249 | 1.45e-26 | 103 |
| MsG0280009623.01.T01 | MTR_8g011370 | 42.188 | 128 | 67 | 2 | 2 | 129 | 130 | 250 | 2.66e-25 | 100 |
| MsG0280009623.01.T01 | MTR_8g011440 | 44.094 | 127 | 67 | 2 | 1 | 126 | 128 | 251 | 3.18e-25 | 100 |
| MsG0280009623.01.T01 | MTR_8g011440 | 42.308 | 130 | 64 | 2 | 1 | 129 | 916 | 1035 | 7.21e-24 | 96.3 |
| MsG0280009623.01.T01 | MTR_8g011410 | 39.416 | 137 | 76 | 2 | 2 | 138 | 130 | 259 | 1.17e-24 | 98.6 |
| MsG0280009623.01.T01 | MTR_8g011377 | 41.441 | 111 | 62 | 1 | 19 | 129 | 345 | 452 | 1.02e-20 | 87.4 |
| MsG0280009623.01.T01 | MTR_8g011377 | 44.578 | 83 | 43 | 1 | 31 | 113 | 14 | 93 | 9.59e-15 | 70.1 |
| MsG0280009623.01.T01 | MTR_5g013130 | 41.441 | 111 | 57 | 3 | 1 | 110 | 126 | 229 | 4.76e-20 | 85.5 |
| MsG0280009623.01.T01 | MTR_5g013070 | 38.739 | 111 | 61 | 2 | 1 | 110 | 126 | 230 | 1.19e-17 | 78.6 |
| MsG0280009623.01.T01 | MTR_4g114250 | 40.625 | 128 | 67 | 3 | 1 | 126 | 137 | 257 | 6.78e-17 | 76.3 |
| MsG0280009623.01.T01 | MTR_5g006160 | 40.678 | 118 | 56 | 5 | 1 | 116 | 121 | 226 | 7.02e-15 | 70.5 |
| MsG0280009623.01.T01 | MTR_4g114270 | 38.211 | 123 | 67 | 3 | 1 | 121 | 137 | 252 | 8.55e-15 | 70.5 |
| MsG0280009623.01.T01 | MTR_4g114240 | 36.937 | 111 | 62 | 3 | 1 | 110 | 139 | 242 | 6.21e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009623.01.T01 | AT5G60900 | 37.594 | 133 | 71 | 3 | 3 | 128 | 138 | 265 | 7.04e-20 | 83.6 |
Find 24 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCAAGCAATTACAGTTAC+AGG | 0.231442 | 2:+55506327 | MsG0280009623.01.T01:CDS |
| AATTGCTTGGCAAATTTATA+AGG | 0.241578 | 2:-55506316 | None:intergenic |
| CAAAGCAACTATTAATTTGA+TGG | 0.318775 | 2:+55506516 | MsG0280009623.01.T01:CDS |
| ATTCTTAGAATCACCACTAA+TGG | 0.329308 | 2:-55506369 | None:intergenic |
| CGTTTGATATTTGACGAATC+AGG | 0.355023 | 2:+55506410 | MsG0280009623.01.T01:CDS |
| TCCGTTGAGAATGCCCCATT+TGG | 0.365489 | 2:-55506482 | None:intergenic |
| TTCAACACCATTACTTGATT+CGG | 0.398012 | 2:-55506203 | None:intergenic |
| TACGCTTCTATGACCATTAG+TGG | 0.420790 | 2:+55506356 | MsG0280009623.01.T01:CDS |
| TTTCTATCAGCAATCCAAAT+GGG | 0.447906 | 2:+55506468 | MsG0280009623.01.T01:CDS |
| AATCAAGTAATGGTGTTGAA+TGG | 0.470273 | 2:+55506206 | MsG0280009623.01.T01:CDS |
| AAACTTCATCTACAAGAGGA+TGG | 0.517032 | 2:+55506278 | MsG0280009623.01.T01:CDS |
| AATGGTCTAATGAATGATGA+TGG | 0.522419 | 2:+55506107 | None:intergenic |
| CGTTCAACTTCTCACAAGGA+AGG | 0.524847 | 2:+55506252 | MsG0280009623.01.T01:CDS |
| GGCCTGTAACTGTAATTGCT+TGG | 0.534016 | 2:-55506329 | None:intergenic |
| AACTTCATCTACAAGAGGAT+GGG | 0.538380 | 2:+55506279 | MsG0280009623.01.T01:CDS |
| ATGGTCATAGAAGCGTAGTA+AGG | 0.551021 | 2:-55506350 | None:intergenic |
| TCCAAATGGGGCATTCTCAA+CGG | 0.598057 | 2:+55506481 | MsG0280009623.01.T01:CDS |
| TTCTATCAGCAATCCAAATG+GGG | 0.611333 | 2:+55506469 | MsG0280009623.01.T01:CDS |
| GTTTAAACTTCATCTACAAG+AGG | 0.614873 | 2:+55506274 | MsG0280009623.01.T01:CDS |
| ATTAGTACCGAATCAAGTAA+TGG | 0.616385 | 2:+55506196 | MsG0280009623.01.T01:CDS |
| TTCGGTACTAATGTGTCAGT+AGG | 0.630587 | 2:-55506185 | None:intergenic |
| TCTAAGAATCAAACAAACGT+TGG | 0.631705 | 2:+55506383 | MsG0280009623.01.T01:CDS |
| GGAAATCTTTCTTCACGACA+AGG | 0.636675 | 2:+55506227 | MsG0280009623.01.T01:CDS |
| GGATCGTTCAACTTCTCACA+AGG | 0.677019 | 2:+55506248 | MsG0280009623.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATAATGAGACATTA+TGG | + | Chr2:55506147-55506166 | MsG0280009623.01.T01:CDS | 20.0% |
| !! | CAAAAATAATGAGACATTAT+GGG | + | Chr2:55506148-55506167 | MsG0280009623.01.T01:CDS | 20.0% |
| !! | TTCTTATACATTGCAAAAAA+AGG | + | Chr2:55506434-55506453 | MsG0280009623.01.T01:CDS | 20.0% |
| ! | AATTGCTTGGCAAATTTATA+AGG | - | Chr2:55506319-55506338 | None:intergenic | 25.0% |
| ! | TATACATTGCAAAAAAAGGA+GGG | + | Chr2:55506438-55506457 | MsG0280009623.01.T01:CDS | 25.0% |
| ! | TTATACATTGCAAAAAAAGG+AGG | + | Chr2:55506437-55506456 | MsG0280009623.01.T01:CDS | 25.0% |
| ATACATTGCAAAAAAAGGAG+GGG | + | Chr2:55506439-55506458 | MsG0280009623.01.T01:CDS | 30.0% | |
| ATTCTTAGAATCACCACTAA+TGG | - | Chr2:55506372-55506391 | None:intergenic | 30.0% | |
| GTTTAAACTTCATCTACAAG+AGG | + | Chr2:55506274-55506293 | MsG0280009623.01.T01:CDS | 30.0% | |
| TCTAAGAATCAAACAAACGT+TGG | + | Chr2:55506383-55506402 | MsG0280009623.01.T01:CDS | 30.0% | |
| TTCAACACCATTACTTGATT+CGG | - | Chr2:55506206-55506225 | None:intergenic | 30.0% | |
| TTTCTATCAGCAATCCAAAT+GGG | + | Chr2:55506468-55506487 | MsG0280009623.01.T01:CDS | 30.0% | |
| ! | AATCAAGTAATGGTGTTGAA+TGG | + | Chr2:55506206-55506225 | MsG0280009623.01.T01:CDS | 30.0% |
| ! | ATTAGTACCGAATCAAGTAA+TGG | + | Chr2:55506196-55506215 | MsG0280009623.01.T01:CDS | 30.0% |
| ! | TTTTCTATCAGCAATCCAAA+TGG | + | Chr2:55506467-55506486 | MsG0280009623.01.T01:CDS | 30.0% |
| AAACTTCATCTACAAGAGGA+TGG | + | Chr2:55506278-55506297 | MsG0280009623.01.T01:CDS | 35.0% | |
| AACTTCATCTACAAGAGGAT+GGG | + | Chr2:55506279-55506298 | MsG0280009623.01.T01:CDS | 35.0% | |
| CGTTTGATATTTGACGAATC+AGG | + | Chr2:55506410-55506429 | MsG0280009623.01.T01:CDS | 35.0% | |
| TTCTATCAGCAATCCAAATG+GGG | + | Chr2:55506469-55506488 | MsG0280009623.01.T01:CDS | 35.0% | |
| GGAAATCTTTCTTCACGACA+AGG | + | Chr2:55506227-55506246 | MsG0280009623.01.T01:CDS | 40.0% | |
| TACGCTTCTATGACCATTAG+TGG | + | Chr2:55506356-55506375 | MsG0280009623.01.T01:CDS | 40.0% | |
| TGCCAAGCAATTACAGTTAC+AGG | + | Chr2:55506327-55506346 | MsG0280009623.01.T01:CDS | 40.0% | |
| TTCGGTACTAATGTGTCAGT+AGG | - | Chr2:55506188-55506207 | None:intergenic | 40.0% | |
| ! | ATGGTCATAGAAGCGTAGTA+AGG | - | Chr2:55506353-55506372 | None:intergenic | 40.0% |
| CGTTCAACTTCTCACAAGGA+AGG | + | Chr2:55506252-55506271 | MsG0280009623.01.T01:CDS | 45.0% | |
| GGATCGTTCAACTTCTCACA+AGG | + | Chr2:55506248-55506267 | MsG0280009623.01.T01:CDS | 45.0% | |
| GGCCTGTAACTGTAATTGCT+TGG | - | Chr2:55506332-55506351 | None:intergenic | 45.0% | |
| ! | TCCAAATGGGGCATTCTCAA+CGG | + | Chr2:55506481-55506500 | MsG0280009623.01.T01:CDS | 45.0% |
| TCCGTTGAGAATGCCCCATT+TGG | - | Chr2:55506485-55506504 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 55506116 | 55506535 | 55506116 | ID=MsG0280009623.01;Name=MsG0280009623.01 |
| Chr2 | mRNA | 55506116 | 55506535 | 55506116 | ID=MsG0280009623.01.T01;Parent=MsG0280009623.01;Name=MsG0280009623.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|139 |
| Chr2 | exon | 55506116 | 55506535 | 55506116 | ID=MsG0280009623.01.T01:exon:5984;Parent=MsG0280009623.01.T01 |
| Chr2 | CDS | 55506116 | 55506535 | 55506116 | ID=MsG0280009623.01.T01:cds;Parent=MsG0280009623.01.T01 |
| Gene Sequence |
| Protein sequence |
