Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009837.01.T01 | MCH94952.1 | 39.806 | 103 | 38 | 3 | 29 | 108 | 50 | 151 | 5.99E-14 | 76.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009837.01.T01 | A0A392N5U5 | 39.806 | 103 | 38 | 3 | 29 | 108 | 50 | 151 | 2.86e-14 | 76.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004463.01 | MsG0280009837.01 | 0.827684 | 1.365241e-54 | 1.327405e-51 |
| MsG0280009837.01 | MsG0480020874.01 | 0.807634 | 4.592692e-50 | 2.571091e-47 |
| MsG0280009837.01 | MsG0480022222.01 | 0.806553 | 7.780521e-50 | 4.235552e-47 |
| MsG0280009837.01 | MsG0480022795.01 | 0.818889 | 1.548851e-52 | 1.173965e-49 |
| MsG0280009837.01 | MsG0580024265.01 | 0.815971 | 7.032929e-52 | 4.920188e-49 |
| MsG0280009837.01 | MsG0780040057.01 | 0.806520 | 7.904342e-50 | 4.299256e-47 |
| MsG0280009837.01 | MsG0780041402.01 | 0.820391 | 7.031111e-53 | 5.556516e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTATTTATTCGGTTCAATT+AGG | 0.211981 | 2:+59152956 | MsG0280009837.01.T01:CDS |
| GACGGATGATGATGATTATA+TGG | 0.298500 | 2:+59153319 | MsG0280009837.01.T01:CDS |
| TGATGATTATATGGGGTATT+AGG | 0.299446 | 2:+59153328 | MsG0280009837.01.T01:CDS |
| ACCTCATGGAGTTTATGATA+TGG | 0.353182 | 2:+59152781 | MsG0280009837.01.T01:CDS |
| AGGTCTATGTTGAAGGTGAT+TGG | 0.374540 | 2:+59153017 | MsG0280009837.01.T01:CDS |
| GTATTTATTCGGTTCAATTA+GGG | 0.385826 | 2:+59152957 | MsG0280009837.01.T01:CDS |
| AGATCCTTGGCTACGTGTGC+AGG | 0.400738 | 2:+59152733 | MsG0280009837.01.T01:CDS |
| AAGGTGATTGGGGTAGCATT+TGG | 0.402947 | 2:+59153029 | MsG0280009837.01.T01:CDS |
| GGTCTATGTTGAAGGTGATT+GGG | 0.412833 | 2:+59153018 | MsG0280009837.01.T01:CDS |
| GAGTTTGCAATGTTGGTTAG+AGG | 0.429963 | 2:+59153217 | MsG0280009837.01.T01:CDS |
| CGAGAGGAGAGGTATTTATT+CGG | 0.440752 | 2:+59152946 | MsG0280009837.01.T01:CDS |
| GTTGATAAGGTCTATGTTGA+AGG | 0.447099 | 2:+59153010 | MsG0280009837.01.T01:CDS |
| TTGCAATGTTGGTTAGAGGC+TGG | 0.466652 | 2:+59153221 | MsG0280009837.01.T01:CDS |
| AAACTTGAAGGGAGTTGATA+AGG | 0.471565 | 2:+59152997 | MsG0280009837.01.T01:CDS |
| GTCTATGTTGAAGGTGATTG+GGG | 0.494297 | 2:+59153019 | MsG0280009837.01.T01:CDS |
| GCCAAGTGAGTTTGCAATGT+TGG | 0.498564 | 2:+59153210 | MsG0280009837.01.T01:CDS |
| ATTGGTAGGAGCTATCTTGT+TGG | 0.500392 | 2:+59153265 | MsG0280009837.01.T01:CDS |
| ACCAACATTGCAAACTCACT+TGG | 0.504719 | 2:-59153211 | None:intergenic |
| ACGGATGATGATGATTATAT+GGG | 0.510187 | 2:+59153320 | MsG0280009837.01.T01:CDS |
| TCATAAACTCCATGAGGTTG+TGG | 0.518101 | 2:-59152776 | None:intergenic |
| TAAGAGTGATGAAAGATCCT+TGG | 0.529032 | 2:+59152720 | None:intergenic |
| TCATTCAGTTGTATTACCAT+TGG | 0.530641 | 2:+59153247 | MsG0280009837.01.T01:CDS |
| GCGTGCTGAAGTACCTCCCA+AGG | 0.549015 | 2:+59153051 | MsG0280009837.01.T01:CDS |
| TACGTGTGCAGGGCTCAGCT+TGG | 0.551927 | 2:+59152744 | MsG0280009837.01.T01:CDS |
| TATTTGCTCACGTGAGGTGG+AGG | 0.563533 | 2:+59153289 | MsG0280009837.01.T01:CDS |
| ATGCAAGCACCACAACCTCA+TGG | 0.582289 | 2:+59152767 | MsG0280009837.01.T01:CDS |
| TTTATGATGAGAAACTTGAA+GGG | 0.587539 | 2:+59152986 | MsG0280009837.01.T01:CDS |
| GGATATTTGCTCACGTGAGG+TGG | 0.587710 | 2:+59153286 | MsG0280009837.01.T01:CDS |
| GATCCTTGGCTACGTGTGCA+GGG | 0.591443 | 2:+59152734 | MsG0280009837.01.T01:CDS |
| TCAGTTGTATTACCATTGGT+AGG | 0.595332 | 2:+59153251 | MsG0280009837.01.T01:CDS |
| GAGCCCTGCACACGTAGCCA+AGG | 0.600029 | 2:-59152737 | None:intergenic |
| GTTGGATATTTGCTCACGTG+AGG | 0.625354 | 2:+59153283 | MsG0280009837.01.T01:CDS |
| AACAAGATAGCTCCTACCAA+TGG | 0.627606 | 2:-59153263 | None:intergenic |
| ATCAGATTACGAACCTTGGG+AGG | 0.628248 | 2:-59153064 | None:intergenic |
| ATAAATACCTCTCCTCTCGT+CGG | 0.649224 | 2:-59152942 | None:intergenic |
| ATGTAGACCGACGAGAGGAG+AGG | 0.652433 | 2:+59152935 | MsG0280009837.01.T01:intron |
| CTTTAATGTAGACCGACGAG+AGG | 0.657157 | 2:+59152930 | MsG0280009837.01.T01:intron |
| TGAGGTGGAGGTCACTGTGA+CGG | 0.682252 | 2:+59153301 | MsG0280009837.01.T01:CDS |
| ACCATATCATAAACTCCATG+AGG | 0.699693 | 2:-59152782 | None:intergenic |
| CGGATGATGATGATTATATG+GGG | 0.702492 | 2:+59153321 | MsG0280009837.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAATACAATGTACGATGAA+TGG | + | Chr2:59153171-59153190 | MsG0280009837.01.T01:intron | 25.0% |
| ! | GTATTTATTCGGTTCAATTA+GGG | + | Chr2:59152957-59152976 | MsG0280009837.01.T01:CDS | 25.0% |
| ! | TTTATGATGAGAAACTTGAA+GGG | + | Chr2:59152986-59153005 | MsG0280009837.01.T01:CDS | 25.0% |
| !! | TTTTATGATGAGAAACTTGA+AGG | + | Chr2:59152985-59153004 | MsG0280009837.01.T01:CDS | 25.0% |
| ACTTCAACATTAGGAATAAG+CGG | - | Chr2:59152905-59152924 | None:intergenic | 30.0% | |
| GGTATTTATTCGGTTCAATT+AGG | + | Chr2:59152956-59152975 | MsG0280009837.01.T01:CDS | 30.0% | |
| TACACAATTCACAATTCACA+GGG | - | Chr2:59153151-59153170 | None:intergenic | 30.0% | |
| TATGGTTGTAAGTGATCTTA+TGG | + | Chr2:59152799-59152818 | MsG0280009837.01.T01:intron | 30.0% | |
| TCATTCAGTTGTATTACCAT+TGG | + | Chr2:59153247-59153266 | MsG0280009837.01.T01:CDS | 30.0% | |
| TTACACAATTCACAATTCAC+AGG | - | Chr2:59153152-59153171 | None:intergenic | 30.0% | |
| TTTACCAACTTGTGTAAAAC+TGG | + | Chr2:59153107-59153126 | MsG0280009837.01.T01:intron | 30.0% | |
| ! | AAAGTATCCACTTCAACATT+AGG | - | Chr2:59152914-59152933 | None:intergenic | 30.0% |
| ! | ACGGATGATGATGATTATAT+GGG | + | Chr2:59153320-59153339 | MsG0280009837.01.T01:CDS | 30.0% |
| ACCATATCATAAACTCCATG+AGG | - | Chr2:59152785-59152804 | None:intergenic | 35.0% | |
| ACCTCATGGAGTTTATGATA+TGG | + | Chr2:59152781-59152800 | MsG0280009837.01.T01:CDS | 35.0% | |
| CAAATCAGATTACGAACCTT+GGG | - | Chr2:59153070-59153089 | None:intergenic | 35.0% | |
| GCTTATTCCTAATGTTGAAG+TGG | + | Chr2:59152904-59152923 | MsG0280009837.01.T01:intron | 35.0% | |
| TCAGTTGTATTACCATTGGT+AGG | + | Chr2:59153251-59153270 | MsG0280009837.01.T01:CDS | 35.0% | |
| ! | CGGATGATGATGATTATATG+GGG | + | Chr2:59153321-59153340 | MsG0280009837.01.T01:CDS | 35.0% |
| ! | GACGGATGATGATGATTATA+TGG | + | Chr2:59153319-59153338 | MsG0280009837.01.T01:CDS | 35.0% |
| ! | GTTGATAAGGTCTATGTTGA+AGG | + | Chr2:59153010-59153029 | MsG0280009837.01.T01:CDS | 35.0% |
| !! | AAACTTGAAGGGAGTTGATA+AGG | + | Chr2:59152997-59153016 | MsG0280009837.01.T01:CDS | 35.0% |
| AACAAGATAGCTCCTACCAA+TGG | - | Chr2:59153266-59153285 | None:intergenic | 40.0% | |
| AATTCACAATTCACAGGGCA+AGG | - | Chr2:59153146-59153165 | None:intergenic | 40.0% | |
| ACCAACATTGCAAACTCACT+TGG | - | Chr2:59153214-59153233 | None:intergenic | 40.0% | |
| AGGTCTATGTTGAAGGTGAT+TGG | + | Chr2:59153017-59153036 | MsG0280009837.01.T01:CDS | 40.0% | |
| ATAAATACCTCTCCTCTCGT+CGG | - | Chr2:59152945-59152964 | None:intergenic | 40.0% | |
| ATCTTATGGTGCCTTGAGAA+AGG | + | Chr2:59152813-59152832 | MsG0280009837.01.T01:intron | 40.0% | |
| ATTGGTAGGAGCTATCTTGT+TGG | + | Chr2:59153265-59153284 | MsG0280009837.01.T01:CDS | 40.0% | |
| CCAAATCAGATTACGAACCT+TGG | - | Chr2:59153071-59153090 | None:intergenic | 40.0% | |
| CCAAGGTTCGTAATCTGATT+TGG | + | Chr2:59153068-59153087 | MsG0280009837.01.T01:intron | 40.0% | |
| GGTCTATGTTGAAGGTGATT+GGG | + | Chr2:59153018-59153037 | MsG0280009837.01.T01:CDS | 40.0% | |
| GTCTATGTTGAAGGTGATTG+GGG | + | Chr2:59153019-59153038 | MsG0280009837.01.T01:CDS | 40.0% | |
| TCATAAACTCCATGAGGTTG+TGG | - | Chr2:59152779-59152798 | None:intergenic | 40.0% | |
| TGTCGCTCAAAATATCCTCT+CGG | + | Chr2:59152877-59152896 | MsG0280009837.01.T01:intron | 40.0% | |
| ! | GAGTTTGCAATGTTGGTTAG+AGG | + | Chr2:59153217-59153236 | MsG0280009837.01.T01:CDS | 40.0% |
| ! | TTGGCCAGTTTTACACAAGT+TGG | - | Chr2:59153114-59153133 | None:intergenic | 40.0% |
| !! | AGGATATTTTGAGCGACATG+AGG | - | Chr2:59152875-59152894 | None:intergenic | 40.0% |
| !! | AGGTTCGTAATCTGATTTGG+AGG | + | Chr2:59153071-59153090 | MsG0280009837.01.T01:intron | 40.0% |
| !! | ATCTGATTTGGAGGATTTGC+TGG | + | Chr2:59153080-59153099 | MsG0280009837.01.T01:intron | 40.0% |
| !! | CGAGAGGAGAGGTATTTATT+CGG | + | Chr2:59152946-59152965 | MsG0280009837.01.T01:CDS | 40.0% |
| !! | TCTGATTTGGAGGATTTGCT+GGG | + | Chr2:59153081-59153100 | MsG0280009837.01.T01:intron | 40.0% |
| AAGGTGATTGGGGTAGCATT+TGG | + | Chr2:59153029-59153048 | MsG0280009837.01.T01:CDS | 45.0% | |
| ATCAGATTACGAACCTTGGG+AGG | - | Chr2:59153067-59153086 | None:intergenic | 45.0% | |
| ATTGCAAACTCACTTGGCAC+TGG | - | Chr2:59153208-59153227 | None:intergenic | 45.0% | |
| CTTTAATGTAGACCGACGAG+AGG | + | Chr2:59152930-59152949 | MsG0280009837.01.T01:intron | 45.0% | |
| GCCAAGTGAGTTTGCAATGT+TGG | + | Chr2:59153210-59153229 | MsG0280009837.01.T01:CDS | 45.0% | |
| GTTGGATATTTGCTCACGTG+AGG | + | Chr2:59153283-59153302 | MsG0280009837.01.T01:CDS | 45.0% | |
| TTATGGTGCCTTGAGAAAGG+AGG | + | Chr2:59152816-59152835 | MsG0280009837.01.T01:intron | 45.0% | |
| ! | TTGCAATGTTGGTTAGAGGC+TGG | + | Chr2:59153221-59153240 | MsG0280009837.01.T01:CDS | 45.0% |
| !! | CTGATTTGGAGGATTTGCTG+GGG | + | Chr2:59153082-59153101 | MsG0280009837.01.T01:intron | 45.0% |
| ACATCCCACCTCCTTTCTCA+AGG | - | Chr2:59152827-59152846 | None:intergenic | 50.0% | |
| ATGCAAGCACCACAACCTCA+TGG | + | Chr2:59152767-59152786 | MsG0280009837.01.T01:CDS | 50.0% | |
| GGATATTTGCTCACGTGAGG+TGG | + | Chr2:59153286-59153305 | MsG0280009837.01.T01:CDS | 50.0% | |
| TAGGAATAAGCGGAACCGAG+AGG | - | Chr2:59152895-59152914 | None:intergenic | 50.0% | |
| TATTTGCTCACGTGAGGTGG+AGG | + | Chr2:59153289-59153308 | MsG0280009837.01.T01:CDS | 50.0% | |
| TTGAGAAAGGAGGTGGGATG+TGG | + | Chr2:59152826-59152845 | MsG0280009837.01.T01:intron | 50.0% | |
| AGATCCTTGGCTACGTGTGC+AGG | + | Chr2:59152733-59152752 | MsG0280009837.01.T01:CDS | 55.0% | |
| ATGTAGACCGACGAGAGGAG+AGG | + | Chr2:59152935-59152954 | MsG0280009837.01.T01:intron | 55.0% | |
| CAGGGCAAGGTACATGCGTT+TGG | - | Chr2:59153133-59153152 | None:intergenic | 55.0% | |
| GATCCTTGGCTACGTGTGCA+GGG | + | Chr2:59152734-59152753 | MsG0280009837.01.T01:CDS | 55.0% | |
| GGTGCCTTGAGAAAGGAGGT+GGG | + | Chr2:59152820-59152839 | MsG0280009837.01.T01:intron | 55.0% | |
| TGAGGTGGAGGTCACTGTGA+CGG | + | Chr2:59153301-59153320 | MsG0280009837.01.T01:CDS | 55.0% | |
| TGGTGCCTTGAGAAAGGAGG+TGG | + | Chr2:59152819-59152838 | MsG0280009837.01.T01:intron | 55.0% | |
| TACGTGTGCAGGGCTCAGCT+TGG | + | Chr2:59152744-59152763 | MsG0280009837.01.T01:CDS | 60.0% | |
| ! | GCGTGCTGAAGTACCTCCCA+AGG | + | Chr2:59153051-59153070 | MsG0280009837.01.T01:CDS | 60.0% |
| GAGCCCTGCACACGTAGCCA+AGG | - | Chr2:59152740-59152759 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 59152728 | 59153349 | 59152728 | ID=MsG0280009837.01;Name=MsG0280009837.01 |
| Chr2 | mRNA | 59152728 | 59153349 | 59152728 | ID=MsG0280009837.01.T01;Parent=MsG0280009837.01;Name=MsG0280009837.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|116 |
| Chr2 | exon | 59152728 | 59152805 | 59152728 | ID=MsG0280009837.01.T01:exon:16568;Parent=MsG0280009837.01.T01 |
| Chr2 | exon | 59152941 | 59153072 | 59152941 | ID=MsG0280009837.01.T01:exon:16569;Parent=MsG0280009837.01.T01 |
| Chr2 | exon | 59153209 | 59153349 | 59153209 | ID=MsG0280009837.01.T01:exon:16570;Parent=MsG0280009837.01.T01 |
| Chr2 | CDS | 59152728 | 59152805 | 59152728 | ID=MsG0280009837.01.T01:cds;Parent=MsG0280009837.01.T01 |
| Chr2 | CDS | 59152941 | 59153072 | 59152941 | ID=MsG0280009837.01.T01:cds;Parent=MsG0280009837.01.T01 |
| Chr2 | CDS | 59153209 | 59153349 | 59153209 | ID=MsG0280009837.01.T01:cds;Parent=MsG0280009837.01.T01 |
| Gene Sequence |
| Protein sequence |
