Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010264.01.T01 | XP_024632293.1 | 95.588 | 68 | 3 | 0 | 1 | 68 | 1 | 68 | 4.75E-39 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010264.01.T01 | A0A396JAI0 | 95.588 | 68 | 3 | 0 | 1 | 68 | 1 | 68 | 2.27e-39 | 133 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010264.01 | MsG0280011361.01 | 0.808879 | 2.492841e-50 | 1.441852e-47 |
| MsG0280010264.01 | MsG0580029310.01 | 0.801989 | 6.939841e-49 | 3.360719e-46 |
| MsG0280010264.01 | MsG0680031468.01 | 0.817527 | 3.149411e-52 | 2.299084e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010264.01.T01 | MTR_2g090110 | 72.059 | 68 | 19 | 0 | 1 | 68 | 1 | 68 | 1.71e-31 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010264.01.T01 | AT3G15534 | 70.588 | 68 | 20 | 0 | 1 | 68 | 1 | 68 | 5.32e-32 | 105 |
| MsG0280010264.01.T01 | AT1G52855 | 66.176 | 68 | 23 | 0 | 1 | 68 | 1 | 68 | 8.59e-30 | 100 |
| MsG0280010264.01.T01 | AT2G20835 | 69.118 | 68 | 21 | 0 | 1 | 68 | 1 | 68 | 1.61e-29 | 99.8 |
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTTCTATTGTAAATCT+TGG | 0.226905 | 2:+66989221 | MsG0280010264.01.T01:CDS |
| TTCCAAGATTTACAATAGAA+AGG | 0.406315 | 2:-66989223 | None:intergenic |
| ACCACTGCGACACCGTGAAC+AGG | 0.448408 | 2:-66989155 | None:intergenic |
| AAAGATACGGCGGAGCAAAC+CGG | 0.451907 | 2:+66989123 | MsG0280010264.01.T01:CDS |
| ACGGCGGAGCAAACCGGTGG+CGG | 0.455842 | 2:+66989129 | MsG0280010264.01.T01:CDS |
| CGTGAACAGGTTCCCGCCAC+CGG | 0.490778 | 2:-66989142 | None:intergenic |
| CGGTGGCGGGAACCTGTTCA+CGG | 0.536826 | 2:+66989143 | MsG0280010264.01.T01:CDS |
| TTGATCAGAAAATAAAGATA+CGG | 0.541218 | 2:+66989110 | MsG0280010264.01.T01:CDS |
| CGGCGGAGCAAACCGGTGGC+GGG | 0.552086 | 2:+66989130 | MsG0280010264.01.T01:CDS |
| ACCTGTTCACGGTGTCGCAG+TGG | 0.585377 | 2:+66989154 | MsG0280010264.01.T01:CDS |
| GTGTCGCAGTGGTGCTAGTG+TGG | 0.613930 | 2:+66989165 | MsG0280010264.01.T01:CDS |
| ATCAGAAAATAAAGATACGG+CGG | 0.658033 | 2:+66989113 | MsG0280010264.01.T01:CDS |
| GATACGGCGGAGCAAACCGG+TGG | 0.688195 | 2:+66989126 | MsG0280010264.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGATCAGAAAATAAAGATA+CGG | + | Chr2:66989110-66989129 | MsG0280010264.01.T01:CDS | 20.0% |
| ! | TTCCAAGATTTACAATAGAA+AGG | - | Chr2:66989226-66989245 | None:intergenic | 25.0% |
| ! | TTCCTTTCTATTGTAAATCT+TGG | + | Chr2:66989221-66989240 | MsG0280010264.01.T01:CDS | 25.0% |
| ATCAGAAAATAAAGATACGG+CGG | + | Chr2:66989113-66989132 | MsG0280010264.01.T01:CDS | 30.0% | |
| !! | GCTATTATATGCACTTTTTG+TGG | + | Chr2:66989247-66989266 | MsG0280010264.01.T01:CDS | 30.0% |
| AAAGATACGGCGGAGCAAAC+CGG | + | Chr2:66989123-66989142 | MsG0280010264.01.T01:CDS | 50.0% | |
| ACCACTGCGACACCGTGAAC+AGG | - | Chr2:66989158-66989177 | None:intergenic | 60.0% | |
| ! | GTGTCGCAGTGGTGCTAGTG+TGG | + | Chr2:66989165-66989184 | MsG0280010264.01.T01:CDS | 60.0% |
| !! | ACCTGTTCACGGTGTCGCAG+TGG | + | Chr2:66989154-66989173 | MsG0280010264.01.T01:CDS | 60.0% |
| CGTGAACAGGTTCCCGCCAC+CGG | - | Chr2:66989145-66989164 | None:intergenic | 65.0% | |
| GATACGGCGGAGCAAACCGG+TGG | + | Chr2:66989126-66989145 | MsG0280010264.01.T01:CDS | 65.0% | |
| !! | CGGTGGCGGGAACCTGTTCA+CGG | + | Chr2:66989143-66989162 | MsG0280010264.01.T01:CDS | 65.0% |
| ACGGCGGAGCAAACCGGTGG+CGG | + | Chr2:66989129-66989148 | MsG0280010264.01.T01:CDS | 70.0% | |
| CGGCGGAGCAAACCGGTGGC+GGG | + | Chr2:66989130-66989149 | MsG0280010264.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 66989088 | 66989294 | 66989088 | ID=MsG0280010264.01;Name=MsG0280010264.01 |
| Chr2 | mRNA | 66989088 | 66989294 | 66989088 | ID=MsG0280010264.01.T01;Parent=MsG0280010264.01;Name=MsG0280010264.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|0|1|0|68 |
| Chr2 | exon | 66989088 | 66989294 | 66989088 | ID=MsG0280010264.01.T01:exon:5485;Parent=MsG0280010264.01.T01 |
| Chr2 | CDS | 66989088 | 66989294 | 66989088 | ID=MsG0280010264.01.T01:cds;Parent=MsG0280010264.01.T01 |
| Gene Sequence |
| Protein sequence |
