AlfalfaGEDB | MsG0280010272.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010272.01

KEGG: K12486
GO: GO:0005096
PFAM: PF01412

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010272.01.T01	AES66726.1	97.163	141	4	0	114	254	16	156	1.81E-91	287

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010272.01.T01	Q9FL69	59.398	133	42	3	114	239	67	194	1.99E-40	149

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010272.01.T01	G7IR45	97.163	141	4	0	114	254	16	156	8.65e-92	287

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005873.01	MsG0280010272.01	0.832915	7.195989e-56	8.158253e-53
MsG0280006950.01	MsG0280010272.01	0.816001	6.925259e-52	4.848830e-49
MsG0280008854.01	MsG0280010272.01	0.809521	1.816113e-50	1.068645e-47
MsG0280010272.01	MsG0380016386.01	0.811036	8.557263e-51	5.241392e-48
MsG0280010272.01	MsG0380017013.01	0.800714	1.266303e-48	5.937335e-46
MsG0280010272.01	MsG0480018125.01	0.846477	2.119088e-59	3.652460e-56
MsG0280010272.01	MsG0480018851.01	0.819657	1.035118e-52	8.014421e-50
MsG0280010272.01	MsG0580030096.01	0.800011	1.760516e-48	8.108899e-46
MsG0280010272.01	MsG0780041664.01	0.811104	8.271725e-51	5.075364e-48
MsG0280010272.01	MsG0780041761.01	0.825618	4.248843e-54	3.892700e-51

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010272.01.T01	MTR_2g080950	97.163	141	4	0	114	254	16	156	2.19e-95	287
MsG0280010272.01.T01	MTR_2g080950	97.163	141	4	0	114	254	67	207	8.41e-95	287
MsG0280010272.01.T01	MTR_2g080950	96.694	121	4	0	134	254	1	121	1.03e-79	246
MsG0280010272.01.T01	MTR_8g464390	70.213	141	31	1	114	254	16	145	5.99e-59	193
MsG0280010272.01.T01	MTR_8g464390	70.213	141	31	1	114	254	67	196	1.03e-58	194
MsG0280010272.01.T01	MTR_2g018850	62.136	103	28	2	1	92	455	557	4.96e-31	122
MsG0280010272.01.T01	MTR_8g464080	35.417	144	62	1	1	113	640	783	1.63e-21	94.4
MsG0280010272.01.T01	MTR_6g080710	63.793	58	19	1	114	169	67	124	1.95e-19	85.9
MsG0280010272.01.T01	MTR_1g088620	92.683	41	3	0	75	115	31	71	1.44e-18	83.6
MsG0280010272.01.T01	MTR_6g086675	37.037	108	37	1	20	96	38	145	1.75e-17	78.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010272.01.T01	AT5G54310	59.398	133	42	3	114	239	67	194	2.03e-41	149
MsG0280010272.01.T01	AT3G17660	65.517	58	20	0	114	171	67	124	9.12e-22	90.1
MsG0280010272.01.T01	AT3G17660	65.517	58	20	0	114	171	16	73	1.87e-21	89.7
MsG0280010272.01.T01	AT3G17660	65.517	58	20	0	114	171	67	124	3.32e-21	89.7
MsG0280010272.01.T01	AT3G17660	65.517	58	20	0	114	171	67	124	4.08e-21	90.1

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Find 83 sgRNAs with CRISPR-Local

Find 391 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATTCTCAGCAACAG+TGG	0.676236	2:-67089431	None:intergenic
AAAGGTATGAAGACAAGAGA+TGG	0.503168	2:+67089308	MsG0280010272.01.T01:intron
AACGTGTATAAGGGTATTCG+AGG	0.534384	2:+67084529	MsG0280010272.01.T01:CDS
AAGACAAGAGATGGGTTTCA+AGG	0.489668	2:+67089317	MsG0280010272.01.T01:CDS
AAGAGATGGGTTTCAAGGGA+TGG	0.525253	2:+67089322	MsG0280010272.01.T01:CDS
AAGGTATGAAGACAAGAGAT+GGG	0.572848	2:+67089309	MsG0280010272.01.T01:intron
AAGTTTCAGCTTGTTGTTGC+GGG	0.320842	2:-67089707	None:intergenic
AATCGCTGTTGCAGATCGAA+AGG	0.609726	2:+67084402	MsG0280010272.01.T01:CDS
AATCTCCTTCACATCAGCAG+AGG	0.669955	2:+67089380	MsG0280010272.01.T01:CDS
ACAACATTCCTAAAGAAAAG+TGG	0.542645	2:+67084440	MsG0280010272.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACCTCCAAAAAGGAAATA+TGG	-	Chr2:67087040-67087059	None:intergenic	30.0%
AAAGGAAATATGGGAAACTA+AGG	-	Chr2:67087030-67087049	None:intergenic	30.0%
AACCTCCAAAAAGGAAATAT+GGG	-	Chr2:67087039-67087058	None:intergenic	30.0%
AACTAGTATATCTTTCCCAT+AGG	-	Chr2:67086951-67086970	None:intergenic	30.0%
AAGCTAAGTATGTATTTGTC+TGG	+	Chr2:67087785-67087804	MsG0280010272.01.T01:intron	30.0%
AAGGAAATATGGGAAACTAA+GGG	-	Chr2:67087029-67087048	None:intergenic	30.0%
ACAAAAGAAAAAACATTGCG+AGG	+	Chr2:67087368-67087387	MsG0280010272.01.T01:intron	30.0%
ACAACATTCCTAAAGAAAAG+TGG	+	Chr2:67084440-67084459	MsG0280010272.01.T01:CDS	30.0%
AGGAGAAGGATTTATTTCAA+TGG	-	Chr2:67085854-67085873	None:intergenic	30.0%
CAAAAGTCATTGGAACTTAT+AGG	-	Chr2:67088876-67088895	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	67084367	67089774	67084367	ID=MsG0280010272.01;Name=MsG0280010272.01
Chr2	mRNA	67084367	67089774	67084367	ID=MsG0280010272.01.T01;Parent=MsG0280010272.01;Name=MsG0280010272.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0.77\|0.8\|1\|0.77\|0.6\|10\|0\|276
Chr2	exon	67089658	67089774	67089658	ID=MsG0280010272.01.T01:exon:7116;Parent=MsG0280010272.01.T01
Chr2	exon	67089312	67089537	67089312	ID=MsG0280010272.01.T01:exon:7115;Parent=MsG0280010272.01.T01
Chr2	exon	67089109	67089199	67089109	ID=MsG0280010272.01.T01:exon:7114;Parent=MsG0280010272.01.T01
Chr2	exon	67088938	67088992	67088938	ID=MsG0280010272.01.T01:exon:7113;Parent=MsG0280010272.01.T01
Chr2	exon	67084683	67084800	67084683	ID=MsG0280010272.01.T01:exon:7112;Parent=MsG0280010272.01.T01
Chr2	exon	67084367	67084590	67084367	ID=MsG0280010272.01.T01:exon:7111;Parent=MsG0280010272.01.T01
Chr2	CDS	67084367	67084590	67084367	ID=MsG0280010272.01.T01:cds;Parent=MsG0280010272.01.T01
Chr2	CDS	67084683	67084800	67084683	ID=MsG0280010272.01.T01:cds;Parent=MsG0280010272.01.T01
Chr2	CDS	67088938	67088992	67088938	ID=MsG0280010272.01.T01:cds;Parent=MsG0280010272.01.T01
Chr2	CDS	67089109	67089199	67089109	ID=MsG0280010272.01.T01:cds;Parent=MsG0280010272.01.T01
Chr2	CDS	67089312	67089537	67089312	ID=MsG0280010272.01.T01:cds;Parent=MsG0280010272.01.T01
Chr2	CDS	67089658	67089774	67089658	ID=MsG0280010272.01.T01:cds;Parent=MsG0280010272.01.T01

Gene Sequence

>MsG0280010272.01.T01

ATGAACATCCCAACATTCGAATACTACCGCTCTGAAATCGCTGTTGCAGATCGAAAGGCTTTAGCATGGGTGGACAACATTCCTAAAGAAAAGTGGACTCAATCACATGATGATGGTCGACGATGGGGCCACATGACAAGTAACTTGGTAGAGTCACAAAACAACGTGTATAAGGGTATTCGAGGACTCCCGATCACAGCTATTGTGAAAGCATCATATTACAGGGATGAGGCTCATAAATCCAACAGTCATCATGTCACTCAGTTTGACCGGGATCGGTATACCTTCTCCGTCCGTGAAACCATCGACCATAAAGAAGGATTACCAAAGGGAGAATACAAGGTTCGTTCTGCAACTCTTGACACTTGGCTTCCAGAGCAAGTTGCATTCATTCAATCAATGGGAAACGAGAAAGCAAATAGCTACTGGGAAGCAGAATTACCTCCACATTACGATAGAGTTGGACTTGAAAATTTTATTCGCGCAAAGTATGAAGACAAGAGATGGGTTTCAAGGGATGGGAATCCAAAAACACCTTCTGGACTGCGTGAAGATAAATCTCCTTCACATCAGCAGAGGCCTGCAGAAAATAGTGGACATGGTTATGCCACTGTTGCTGAGAATACTTTTGAAGAAAGGAAGAAAATCCAACCATCAAATGCAGTTCCTGCTACAAGACGTAGGGTTCCAGCTCCTCGCAAGGTACCGGAGCAGGTAACTCCTGCTACCCAACCTCAGCACACTGAGAAAGTTGAACCAGTACCCCGCAACAACAAGCTGAAACTTCAAAGCCGGCTACCGACACAGCTCAAAGTACCCCTCCCAAAGTAG

Protein sequence

>MsG0280010272.01.T01

MNIPTFEYYRSEIAVADRKALAWVDNIPKEKWTQSHDDGRRWGHMTSNLVESQNNVYKGIRGLPITAIVKASYYRDEAHKSNSHHVTQFDRDRYTFSVRETIDHKEGLPKGEYKVRSATLDTWLPEQVAFIQSMGNEKANSYWEAELPPHYDRVGLENFIRAKYEDKRWVSRDGNPKTPSGLREDKSPSHQQRPAENSGHGYATVAENTFEERKKIQPSNAVPATRRRVPAPRKVPEQVTPATQPQHTEKVEPVPRNNKLKLQSRLPTQLKVPLPK*