Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011096.01.T01 | XP_018459315.1 | 45.19 | 343 | 164 | 8 | 1 | 342 | 1 | 320 | 3.78E-93 | 291 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011096.01.T01 | A0A6J0LGH1 | 45.190 | 343 | 164 | 8 | 1 | 342 | 1 | 320 | 1.80e-93 | 291 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011096.01 | MsG0780036201.01 | 0.816076 | 6.663009e-52 | 4.674953e-49 |
| MsG0280011096.01 | MsG0780036202.01 | 0.904053 | 1.832747e-79 | 2.785283e-75 |
| MsG0280011096.01 | MsG0780036874.01 | 0.980064 | 2.304631e-149 | 5.293081e-143 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011096.01.T01 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 6.22e-32 | 118 |
| MsG0280011096.01.T01 | MTR_4g037355 | 38.686 | 137 | 62 | 3 | 155 | 290 | 1 | 116 | 1.66e-21 | 89.4 |
| MsG0280011096.01.T01 | MTR_5g061150 | 33.333 | 111 | 74 | 0 | 40 | 150 | 26 | 136 | 1.24e-17 | 79.0 |
| MsG0280011096.01.T01 | MTR_4g112730 | 56.522 | 69 | 29 | 1 | 143 | 211 | 2 | 69 | 2.96e-16 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATTATTGCCCTCATTAA+TGG | 0.192737 | 2:+79526056 | MsG0280011096.01.T01:CDS |
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 2:+79526076 | MsG0280011096.01.T01:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 2:-79526372 | None:intergenic |
| CAAAAGATCCCAAGATCTTA+TGG | 0.237847 | 2:+79525998 | MsG0280011096.01.T01:CDS |
| GACAAAATTAAAGCAAGATT+TGG | 0.252197 | 2:+79526021 | MsG0280011096.01.T01:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 2:+79525879 | MsG0280011096.01.T01:CDS |
| CACGACCTCTACCACGAAAA+TGG | 0.272842 | 2:-79526488 | None:intergenic |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 2:-79525830 | None:intergenic |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 2:+79526266 | MsG0280011096.01.T01:CDS |
| GAAGAGGTTATATGAATTAT+AGG | 0.339571 | 2:+79526511 | MsG0280011096.01.T01:CDS |
| AGACAGGTAGTGTCATTCTT+TGG | 0.344985 | 2:-79526744 | None:intergenic |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 2:+79525880 | MsG0280011096.01.T01:CDS |
| CGTAGTGGCTATGCTCGTTT+TGG | 0.359061 | 2:+79526432 | MsG0280011096.01.T01:CDS |
| TATTTCTAGAGGGACCTTCC+TGG | 0.364970 | 2:-79526587 | None:intergenic |
| CATAGCCACTACGTCCTCTT+TGG | 0.391081 | 2:-79526422 | None:intergenic |
| ACAAAATTAAAGCAAGATTT+GGG | 0.407053 | 2:+79526022 | MsG0280011096.01.T01:CDS |
| CAAAGAATCTGTGGAGGAAA+AGG | 0.416635 | 2:+79526692 | MsG0280011096.01.T01:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 2:+79526064 | MsG0280011096.01.T01:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.441180 | 2:+79526179 | MsG0280011096.01.T01:CDS |
| AAAAGATCCCAAGATCTTAT+GGG | 0.458748 | 2:+79525999 | MsG0280011096.01.T01:CDS |
| CGTGGTAGAGGTCGTGGAAG+AGG | 0.462783 | 2:+79526495 | MsG0280011096.01.T01:CDS |
| ATCCACCAGTTGCGTCCTAC+AGG | 0.478404 | 2:+79526336 | MsG0280011096.01.T01:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 2:-79526341 | None:intergenic |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 2:+79525952 | MsG0280011096.01.T01:CDS |
| CTGGCGACAACTACATAACC+TGG | 0.502097 | 2:+79525812 | MsG0280011096.01.T01:CDS |
| GATGCGGAAAGAAAGGTCAT+TGG | 0.503149 | 2:+79526628 | MsG0280011096.01.T01:CDS |
| ATGCGGAAAGAAAGGTCATT+GGG | 0.509313 | 2:+79526629 | MsG0280011096.01.T01:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 2:-79526067 | None:intergenic |
| TGTTATAGATGCGGAAAGAA+AGG | 0.513070 | 2:+79526621 | MsG0280011096.01.T01:CDS |
| GGACTAGACAAGATTCTTGA+GGG | 0.521095 | 2:+79525868 | MsG0280011096.01.T01:CDS |
| TTTGATTGGTCATATTTGAG+AGG | 0.522357 | 2:-79526534 | None:intergenic |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 2:+79526241 | MsG0280011096.01.T01:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 2:-79526066 | None:intergenic |
| CGTGGACGAGGTAGAAATCA+TGG | 0.535808 | 2:+79526459 | MsG0280011096.01.T01:CDS |
| AAAATCAGCAGCTTCAAGAC+AGG | 0.547335 | 2:-79526760 | None:intergenic |
| GTCATTGGGCTAAAACATGC+AGG | 0.564978 | 2:+79526643 | MsG0280011096.01.T01:CDS |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 2:+79525846 | MsG0280011096.01.T01:CDS |
| TTCCTGTAGGACGCAACTGG+TGG | 0.566933 | 2:-79526338 | None:intergenic |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 2:+79525847 | MsG0280011096.01.T01:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 2:-79526351 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 2:+79526284 | MsG0280011096.01.T01:CDS |
| ACTATCATCATTATTTCTAG+AGG | 0.581988 | 2:-79526598 | None:intergenic |
| TAAAAGATACAAAGAATCTG+TGG | 0.594054 | 2:+79526683 | MsG0280011096.01.T01:CDS |
| CTATCATCATTATTTCTAGA+GGG | 0.603915 | 2:-79526597 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 2:+79526242 | MsG0280011096.01.T01:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 2:-79526385 | None:intergenic |
| GGGACTAGACAAGATTCTTG+AGG | 0.613207 | 2:+79525867 | MsG0280011096.01.T01:CDS |
| GATGATAGTTGTTATAGATG+CGG | 0.615209 | 2:+79526612 | MsG0280011096.01.T01:CDS |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 2:+79526408 | MsG0280011096.01.T01:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 2:+79526344 | MsG0280011096.01.T01:CDS |
| AAGATACAAAGAATCTGTGG+AGG | 0.669466 | 2:+79526686 | MsG0280011096.01.T01:CDS |
| AATCGCCAAAGAGGACGTAG+TGG | 0.674563 | 2:+79526417 | MsG0280011096.01.T01:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 2:-79525960 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 2:+79525894 | MsG0280011096.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATTAAAGCAAGATTT+GGG | + | Chr2:79526022-79526041 | MsG0280011096.01.T01:CDS | 20.0% |
| !!! | TTCAAAATTTTAAGCATAAC+TGG | + | Chr2:79525793-79525812 | MsG0280011096.01.T01:CDS | 20.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | + | Chr2:79526266-79526285 | MsG0280011096.01.T01:CDS | 25.0% |
| ! | CAATCAAACAAAAATCATCA+GGG | + | Chr2:79526549-79526568 | MsG0280011096.01.T01:CDS | 25.0% |
| ! | GAAGAGGTTATATGAATTAT+AGG | + | Chr2:79526511-79526530 | MsG0280011096.01.T01:CDS | 25.0% |
| ! | GACAAAATTAAAGCAAGATT+TGG | + | Chr2:79526021-79526040 | MsG0280011096.01.T01:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | + | Chr2:79526683-79526702 | MsG0280011096.01.T01:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | + | Chr2:79526150-79526169 | MsG0280011096.01.T01:CDS | 25.0% |
| !!! | ACTATCATCATTATTTCTAG+AGG | - | Chr2:79526601-79526620 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | - | Chr2:79526104-79526123 | None:intergenic | 25.0% |
| !!! | CTATCATCATTATTTCTAGA+GGG | - | Chr2:79526600-79526619 | None:intergenic | 25.0% |
| !!! | TGTTGAAGGAGATTTTAATA+TGG | + | Chr2:79526782-79526801 | MsG0280011096.01.T01:CDS | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | + | Chr2:79525999-79526018 | MsG0280011096.01.T01:CDS | 30.0% | |
| AACAAAAATCATCAGGGTAA+AGG | + | Chr2:79526555-79526574 | MsG0280011096.01.T01:CDS | 30.0% | |
| AGTATTATTGCCCTCATTAA+TGG | + | Chr2:79526056-79526075 | MsG0280011096.01.T01:CDS | 30.0% | |
| CCAATCAAACAAAAATCATC+AGG | + | Chr2:79526548-79526567 | MsG0280011096.01.T01:CDS | 30.0% | |
| GATGATAGTTGTTATAGATG+CGG | + | Chr2:79526612-79526631 | MsG0280011096.01.T01:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | + | Chr2:79526242-79526261 | MsG0280011096.01.T01:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | + | Chr2:79526179-79526198 | MsG0280011096.01.T01:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | + | Chr2:79526241-79526260 | MsG0280011096.01.T01:CDS | 30.0% | |
| TGGAAAAACTATAACTGAAG+TGG | + | Chr2:79526170-79526189 | MsG0280011096.01.T01:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | + | Chr2:79526076-79526095 | MsG0280011096.01.T01:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | - | Chr2:79526069-79526088 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | - | Chr2:79525963-79525982 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | - | Chr2:79526010-79526029 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | - | Chr2:79526009-79526028 | None:intergenic | 30.0% |
| ! | TATCTTTTACACAGATACTC+TGG | - | Chr2:79526672-79526691 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | - | Chr2:79526537-79526556 | None:intergenic | 30.0% |
| ! | TTTTCTTTGCTAATTCCTCT+GGG | - | Chr2:79525912-79525931 | None:intergenic | 30.0% |
| ! | TTTTTCTTTGCTAATTCCTC+TGG | - | Chr2:79525913-79525932 | None:intergenic | 30.0% |
| !! | CCTGATGATTTTTGTTTGAT+TGG | - | Chr2:79526551-79526570 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | + | Chr2:79526573-79526592 | MsG0280011096.01.T01:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | - | Chr2:79526375-79526394 | None:intergenic | 35.0% | |
| AAGATACAAAGAATCTGTGG+AGG | + | Chr2:79526686-79526705 | MsG0280011096.01.T01:CDS | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | + | Chr2:79525846-79525865 | MsG0280011096.01.T01:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | + | Chr2:79525847-79525866 | MsG0280011096.01.T01:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | + | Chr2:79525998-79526017 | MsG0280011096.01.T01:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | - | Chr2:79526070-79526089 | None:intergenic | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | + | Chr2:79526621-79526640 | MsG0280011096.01.T01:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | + | Chr2:79526381-79526400 | MsG0280011096.01.T01:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | + | Chr2:79526382-79526401 | MsG0280011096.01.T01:CDS | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | - | Chr2:79525833-79525852 | None:intergenic | 35.0% |
| AAAATCAGCAGCTTCAAGAC+AGG | - | Chr2:79526763-79526782 | None:intergenic | 40.0% | |
| ACGCCCTTGATTAAAAGTTG+TGG | - | Chr2:79526388-79526407 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | + | Chr2:79525952-79525971 | MsG0280011096.01.T01:CDS | 40.0% | |
| ATGCGGAAAGAAAGGTCATT+GGG | + | Chr2:79526629-79526648 | MsG0280011096.01.T01:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | + | Chr2:79525880-79525899 | MsG0280011096.01.T01:CDS | 40.0% | |
| CAAAGAATCTGTGGAGGAAA+AGG | + | Chr2:79526692-79526711 | MsG0280011096.01.T01:CDS | 40.0% | |
| GGACTAGACAAGATTCTTGA+GGG | + | Chr2:79525868-79525887 | MsG0280011096.01.T01:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | + | Chr2:79526569-79526588 | MsG0280011096.01.T01:CDS | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | + | Chr2:79526215-79526234 | MsG0280011096.01.T01:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | + | Chr2:79526387-79526406 | MsG0280011096.01.T01:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | - | Chr2:79526747-79526766 | None:intergenic | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | + | Chr2:79526212-79526231 | MsG0280011096.01.T01:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | + | Chr2:79526064-79526083 | MsG0280011096.01.T01:CDS | 40.0% |
| !!! | GAAGCTGCTGATTTTGTTGA+AGG | + | Chr2:79526768-79526787 | MsG0280011096.01.T01:CDS | 40.0% |
| GATGCGGAAAGAAAGGTCAT+TGG | + | Chr2:79526628-79526647 | MsG0280011096.01.T01:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | + | Chr2:79525879-79525898 | MsG0280011096.01.T01:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | - | Chr2:79526354-79526373 | None:intergenic | 45.0% | |
| GGGACTAGACAAGATTCTTG+AGG | + | Chr2:79525867-79525886 | MsG0280011096.01.T01:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | + | Chr2:79526408-79526427 | MsG0280011096.01.T01:CDS | 45.0% | |
| GTCATTGGGCTAAAACATGC+AGG | + | Chr2:79526643-79526662 | MsG0280011096.01.T01:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | + | Chr2:79526284-79526303 | MsG0280011096.01.T01:CDS | 45.0% | |
| ! | CATGGTCGAAACCATTTTCG+TGG | + | Chr2:79526477-79526496 | MsG0280011096.01.T01:CDS | 45.0% |
| ! | CGAAACCATTTTCGTGGTAG+AGG | + | Chr2:79526483-79526502 | MsG0280011096.01.T01:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | + | Chr2:79526390-79526409 | MsG0280011096.01.T01:CDS | 45.0% |
| !! | TATTTCTAGAGGGACCTTCC+TGG | - | Chr2:79526590-79526609 | None:intergenic | 45.0% |
| AATCGCCAAAGAGGACGTAG+TGG | + | Chr2:79526417-79526436 | MsG0280011096.01.T01:CDS | 50.0% | |
| CACGACCTCTACCACGAAAA+TGG | - | Chr2:79526491-79526510 | None:intergenic | 50.0% | |
| CATAGCCACTACGTCCTCTT+TGG | - | Chr2:79526425-79526444 | None:intergenic | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | + | Chr2:79526459-79526478 | MsG0280011096.01.T01:CDS | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | + | Chr2:79525812-79525831 | MsG0280011096.01.T01:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | + | Chr2:79526344-79526363 | MsG0280011096.01.T01:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | - | Chr2:79526344-79526363 | None:intergenic | 50.0% | |
| ! | AGTGGCTATGCTCGTTTTGG+TGG | + | Chr2:79526435-79526454 | MsG0280011096.01.T01:CDS | 50.0% |
| ! | CATTTTCGTGGTAGAGGTCG+TGG | + | Chr2:79526489-79526508 | MsG0280011096.01.T01:CDS | 50.0% |
| ! | CGTAGTGGCTATGCTCGTTT+TGG | + | Chr2:79526432-79526451 | MsG0280011096.01.T01:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | + | Chr2:79526441-79526460 | MsG0280011096.01.T01:CDS | 50.0% |
| ATCCACCAGTTGCGTCCTAC+AGG | + | Chr2:79526336-79526355 | MsG0280011096.01.T01:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | - | Chr2:79526341-79526360 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | + | Chr2:79525894-79525913 | MsG0280011096.01.T01:CDS | 55.0% |
| CGTGGTAGAGGTCGTGGAAG+AGG | + | Chr2:79526495-79526514 | MsG0280011096.01.T01:CDS | 60.0% | |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | + | Chr2:79526447-79526466 | MsG0280011096.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 79525772 | 79526809 | 79525772 | ID=MsG0280011096.01;Name=MsG0280011096.01 |
| Chr2 | mRNA | 79525772 | 79526809 | 79525772 | ID=MsG0280011096.01.T01;Parent=MsG0280011096.01;Name=MsG0280011096.01.T01;_AED=0.47;_eAED=0.57;_QI=0|-1|0|1|-1|1|1|0|345 |
| Chr2 | exon | 79525772 | 79526809 | 79525772 | ID=MsG0280011096.01.T01:exon:10008;Parent=MsG0280011096.01.T01 |
| Chr2 | CDS | 79525772 | 79526809 | 79525772 | ID=MsG0280011096.01.T01:cds;Parent=MsG0280011096.01.T01 |
| Gene Sequence |
| Protein sequence |
