Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036202.01.T01 | XP_018459315.1 | 45.161 | 217 | 101 | 5 | 4 | 220 | 122 | 320 | 9.16E-52 | 180 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036202.01.T01 | A0A6J0LGH1 | 45.161 | 217 | 101 | 5 | 4 | 220 | 122 | 320 | 4.37e-52 | 180 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011096.01 | MsG0780036202.01 | 0.904053 | 1.832747e-79 | 2.785283e-75 |
| MsG0780036201.01 | MsG0780036202.01 | 0.830910 | 2.250574e-55 | 2.404446e-52 |
| MsG0780036202.01 | MsG0780036874.01 | 0.914021 | 3.124595e-84 | 7.605118e-80 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036202.01.T01 | MTR_4g037355 | 38.168 | 131 | 59 | 3 | 33 | 162 | 1 | 110 | 6.00e-21 | 85.5 |
| MsG0780036202.01.T01 | MTR_4g112730 | 56.338 | 71 | 30 | 1 | 21 | 91 | 2 | 71 | 2.45e-18 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 7:-4633815 | None:intergenic |
| CACGACCTCTACCACGAAAA+TGG | 0.272842 | 7:-4633931 | None:intergenic |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 7:+4633709 | MsG0780036202.01.T01:CDS |
| GAAGAGGTTATATGAATTAT+AGG | 0.339571 | 7:+4633954 | MsG0780036202.01.T01:CDS |
| AGACAGGTAGTGTCATTCTT+TGG | 0.344985 | 7:-4634187 | None:intergenic |
| CGTAGTGGCTATGCTCGTTT+TGG | 0.359061 | 7:+4633875 | MsG0780036202.01.T01:CDS |
| TATTTCTAGAGGGACCTTCC+TGG | 0.364970 | 7:-4634030 | None:intergenic |
| CATAGCCACTACGTCCTCTT+TGG | 0.391081 | 7:-4633865 | None:intergenic |
| CAAAGAATCTGTGGAGGAAA+AGG | 0.416635 | 7:+4634135 | MsG0780036202.01.T01:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.441180 | 7:+4633622 | MsG0780036202.01.T01:CDS |
| CGTGGTAGAGGTCGTGGAAG+AGG | 0.462783 | 7:+4633938 | MsG0780036202.01.T01:CDS |
| AACCACCAGTTGCGTCCTAC+AGG | 0.478404 | 7:+4633779 | MsG0780036202.01.T01:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 7:-4633784 | None:intergenic |
| GTTTAGATGCAAAGAAAGAA+AGG | 0.481148 | 7:+4634065 | MsG0780036202.01.T01:CDS |
| TTTGATTGGTCATATTTGAG+AGG | 0.522357 | 7:-4633977 | None:intergenic |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 7:+4633684 | MsG0780036202.01.T01:CDS |
| ATGCAAAGAAAGAAAGGTCA+TGG | 0.526773 | 7:+4634071 | MsG0780036202.01.T01:CDS |
| CGTGGACGAGGTAGAAATCA+TGG | 0.535808 | 7:+4633902 | MsG0780036202.01.T01:CDS |
| AAAATCAGCAGCTTCAAGAC+AGG | 0.547335 | 7:-4634203 | None:intergenic |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 7:-4633794 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 7:+4633727 | MsG0780036202.01.T01:CDS |
| ACTATCATCATTATTTCTAG+AGG | 0.581988 | 7:-4634041 | None:intergenic |
| TTCCTGTAGGACGCAACTGG+TGG | 0.593030 | 7:-4633781 | None:intergenic |
| TAAAAGATACAAAGAATCTG+TGG | 0.594054 | 7:+4634126 | MsG0780036202.01.T01:CDS |
| TGCAAAGAAAGAAAGGTCAT+GGG | 0.602168 | 7:+4634072 | MsG0780036202.01.T01:CDS |
| CTATCATCATTATTTCTAGA+GGG | 0.603915 | 7:-4634040 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 7:+4633685 | MsG0780036202.01.T01:CDS |
| GGTCATGGGCTAAAACATGC+AGG | 0.607005 | 7:+4634086 | MsG0780036202.01.T01:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 7:-4633828 | None:intergenic |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 7:+4633851 | MsG0780036202.01.T01:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 7:+4633787 | MsG0780036202.01.T01:CDS |
| AAGATACAAAGAATCTGTGG+AGG | 0.669466 | 7:+4634129 | MsG0780036202.01.T01:CDS |
| AATCGCCAAAGAGGACGTAG+TGG | 0.674563 | 7:+4633860 | MsG0780036202.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTTACTGAATACTCTGATT+TGG | + | Chr7:4633709-4633728 | MsG0780036202.01.T01:CDS | 25.0% |
| ! | CAATCAAACAAAAATCATCA+GGG | + | Chr7:4633992-4634011 | MsG0780036202.01.T01:CDS | 25.0% |
| ! | GAAGAGGTTATATGAATTAT+AGG | + | Chr7:4633954-4633973 | MsG0780036202.01.T01:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | + | Chr7:4634126-4634145 | MsG0780036202.01.T01:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | + | Chr7:4633593-4633612 | MsG0780036202.01.T01:CDS | 25.0% |
| !! | TTGTTGAAGGAGATTTTATA+TGG | + | Chr7:4634224-4634243 | MsG0780036202.01.T01:CDS | 25.0% |
| !!! | ACTATCATCATTATTTCTAG+AGG | - | Chr7:4634044-4634063 | None:intergenic | 25.0% |
| !!! | CTATCATCATTATTTCTAGA+GGG | - | Chr7:4634043-4634062 | None:intergenic | 25.0% |
| AACAAAAATCATCAGGGTAA+AGG | + | Chr7:4633998-4634017 | MsG0780036202.01.T01:CDS | 30.0% | |
| CCAATCAAACAAAAATCATC+AGG | + | Chr7:4633991-4634010 | MsG0780036202.01.T01:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | + | Chr7:4633685-4633704 | MsG0780036202.01.T01:CDS | 30.0% | |
| GTTTAGATGCAAAGAAAGAA+AGG | + | Chr7:4634065-4634084 | MsG0780036202.01.T01:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | + | Chr7:4633622-4633641 | MsG0780036202.01.T01:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | + | Chr7:4633684-4633703 | MsG0780036202.01.T01:CDS | 30.0% | |
| TGGAAAAACTATAACTGAAG+TGG | + | Chr7:4633613-4633632 | MsG0780036202.01.T01:CDS | 30.0% | |
| ! | TATCTTTTACACAGATACTC+TGG | - | Chr7:4634115-4634134 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | - | Chr7:4633980-4633999 | None:intergenic | 30.0% |
| !! | CCTGATGATTTTTGTTTGAT+TGG | - | Chr7:4633994-4634013 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | + | Chr7:4634016-4634035 | MsG0780036202.01.T01:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | - | Chr7:4633818-4633837 | None:intergenic | 35.0% | |
| AAGATACAAAGAATCTGTGG+AGG | + | Chr7:4634129-4634148 | MsG0780036202.01.T01:CDS | 35.0% | |
| ATGCAAAGAAAGAAAGGTCA+TGG | + | Chr7:4634071-4634090 | MsG0780036202.01.T01:CDS | 35.0% | |
| TGCAAAGAAAGAAAGGTCAT+GGG | + | Chr7:4634072-4634091 | MsG0780036202.01.T01:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | + | Chr7:4633824-4633843 | MsG0780036202.01.T01:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | + | Chr7:4633825-4633844 | MsG0780036202.01.T01:CDS | 35.0% |
| AAAATCAGCAGCTTCAAGAC+AGG | - | Chr7:4634206-4634225 | None:intergenic | 40.0% | |
| ACGCCCTTGATTAAAAGTTG+TGG | - | Chr7:4633831-4633850 | None:intergenic | 40.0% | |
| CAAAGAATCTGTGGAGGAAA+AGG | + | Chr7:4634135-4634154 | MsG0780036202.01.T01:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | + | Chr7:4634012-4634031 | MsG0780036202.01.T01:CDS | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | + | Chr7:4633658-4633677 | MsG0780036202.01.T01:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | + | Chr7:4633830-4633849 | MsG0780036202.01.T01:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | - | Chr7:4634190-4634209 | None:intergenic | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | + | Chr7:4633655-4633674 | MsG0780036202.01.T01:CDS | 40.0% |
| !!! | GAAGCTGCTGATTTTGTTGA+AGG | + | Chr7:4634211-4634230 | MsG0780036202.01.T01:CDS | 40.0% |
| GGATATGCCATTGTTCCTGT+AGG | - | Chr7:4633797-4633816 | None:intergenic | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | + | Chr7:4633851-4633870 | MsG0780036202.01.T01:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | + | Chr7:4633727-4633746 | MsG0780036202.01.T01:CDS | 45.0% | |
| ! | CATGGTCGAAACCATTTTCG+TGG | + | Chr7:4633920-4633939 | MsG0780036202.01.T01:CDS | 45.0% |
| ! | CGAAACCATTTTCGTGGTAG+AGG | + | Chr7:4633926-4633945 | MsG0780036202.01.T01:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | + | Chr7:4633833-4633852 | MsG0780036202.01.T01:CDS | 45.0% |
| !! | TATTTCTAGAGGGACCTTCC+TGG | - | Chr7:4634033-4634052 | None:intergenic | 45.0% |
| AATCGCCAAAGAGGACGTAG+TGG | + | Chr7:4633860-4633879 | MsG0780036202.01.T01:CDS | 50.0% | |
| CACGACCTCTACCACGAAAA+TGG | - | Chr7:4633934-4633953 | None:intergenic | 50.0% | |
| CATAGCCACTACGTCCTCTT+TGG | - | Chr7:4633868-4633887 | None:intergenic | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | + | Chr7:4633902-4633921 | MsG0780036202.01.T01:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | + | Chr7:4633787-4633806 | MsG0780036202.01.T01:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | - | Chr7:4633787-4633806 | None:intergenic | 50.0% | |
| ! | AGTGGCTATGCTCGTTTTGG+TGG | + | Chr7:4633878-4633897 | MsG0780036202.01.T01:CDS | 50.0% |
| ! | CATTTTCGTGGTAGAGGTCG+TGG | + | Chr7:4633932-4633951 | MsG0780036202.01.T01:CDS | 50.0% |
| ! | CGTAGTGGCTATGCTCGTTT+TGG | + | Chr7:4633875-4633894 | MsG0780036202.01.T01:CDS | 50.0% |
| ! | GGTCATGGGCTAAAACATGC+AGG | + | Chr7:4634086-4634105 | MsG0780036202.01.T01:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | + | Chr7:4633884-4633903 | MsG0780036202.01.T01:CDS | 50.0% |
| AACCACCAGTTGCGTCCTAC+AGG | + | Chr7:4633779-4633798 | MsG0780036202.01.T01:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | - | Chr7:4633784-4633803 | None:intergenic | 55.0% | |
| CGTGGTAGAGGTCGTGGAAG+AGG | + | Chr7:4633938-4633957 | MsG0780036202.01.T01:CDS | 60.0% | |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | + | Chr7:4633890-4633909 | MsG0780036202.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 4633581 | 4634255 | 4633581 | ID=MsG0780036202.01;Name=MsG0780036202.01 |
| Chr7 | mRNA | 4633581 | 4634255 | 4633581 | ID=MsG0780036202.01.T01;Parent=MsG0780036202.01;Name=MsG0780036202.01.T01;_AED=0.57;_eAED=0.62;_QI=0|-1|0|1|-1|1|1|0|224 |
| Chr7 | exon | 4633581 | 4634255 | 4633581 | ID=MsG0780036202.01.T01:exon:2589;Parent=MsG0780036202.01.T01 |
| Chr7 | CDS | 4633581 | 4634255 | 4633581 | ID=MsG0780036202.01.T01:cds;Parent=MsG0780036202.01.T01 |
| Gene Sequence |
| Protein sequence |
