Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036874.01.T01 | XP_018459315.1 | 45.481 | 343 | 163 | 8 | 1 | 342 | 1 | 320 | 1.53E-94 | 295 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036874.01.T01 | A0A6J0LGH1 | 45.481 | 343 | 163 | 8 | 1 | 342 | 1 | 320 | 7.31e-95 | 295 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011096.01 | MsG0780036874.01 | 0.980064 | 2.304631e-149 | 5.293081e-143 |
| MsG0780036201.01 | MsG0780036874.01 | 0.834457 | 2.964616e-56 | 3.520403e-53 |
| MsG0780036202.01 | MsG0780036874.01 | 0.914021 | 3.124595e-84 | 7.605118e-80 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036874.01.T01 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 6.83e-32 | 117 |
| MsG0780036874.01.T01 | MTR_4g037355 | 39.416 | 137 | 61 | 3 | 155 | 290 | 1 | 116 | 1.11e-22 | 92.4 |
| MsG0780036874.01.T01 | MTR_5g061150 | 33.333 | 111 | 74 | 0 | 40 | 150 | 26 | 136 | 1.38e-17 | 78.6 |
| MsG0780036874.01.T01 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 1.83e-17 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATTATTGCCCTCATTAA+TGG | 0.192737 | 7:-15472076 | MsG0780036874.01.T01:CDS |
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 7:-15472056 | MsG0780036874.01.T01:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 7:+15471760 | None:intergenic |
| CAAAAGATCCCAAGATCTTA+TGG | 0.237847 | 7:-15472134 | MsG0780036874.01.T01:CDS |
| GACAAAATTAAAGCAAGATT+TGG | 0.252197 | 7:-15472111 | MsG0780036874.01.T01:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 7:-15472253 | MsG0780036874.01.T01:CDS |
| CACGACCTCTACCACGAAAA+TGG | 0.272842 | 7:+15471644 | None:intergenic |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 7:+15472302 | None:intergenic |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 7:-15471866 | MsG0780036874.01.T01:CDS |
| GAAGAGGTTATATGAATTAT+AGG | 0.339571 | 7:-15471621 | MsG0780036874.01.T01:CDS |
| AGACAGGTAGTGTCATTCTT+TGG | 0.344985 | 7:+15471388 | None:intergenic |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 7:-15472252 | MsG0780036874.01.T01:CDS |
| CGTAGTGGCTATGCTCGTTT+TGG | 0.359061 | 7:-15471700 | MsG0780036874.01.T01:CDS |
| TATTTCTAGAGGGACCTTCC+TGG | 0.364970 | 7:+15471545 | None:intergenic |
| CATAGCCACTACGTCCTCTT+TGG | 0.391081 | 7:+15471710 | None:intergenic |
| ACAAAATTAAAGCAAGATTT+GGG | 0.407053 | 7:-15472110 | MsG0780036874.01.T01:CDS |
| CAAAGAATCTGTGGAGGAAA+AGG | 0.416635 | 7:-15471440 | MsG0780036874.01.T01:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 7:-15472068 | MsG0780036874.01.T01:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.441180 | 7:-15471953 | MsG0780036874.01.T01:CDS |
| AAAAGATCCCAAGATCTTAT+GGG | 0.458748 | 7:-15472133 | MsG0780036874.01.T01:CDS |
| CGTGGTAGAGGTCGTGGAAG+AGG | 0.462783 | 7:-15471637 | MsG0780036874.01.T01:CDS |
| AACCACCAGTTGCGTCCTAC+AGG | 0.478404 | 7:-15471796 | MsG0780036874.01.T01:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 7:+15471791 | None:intergenic |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 7:-15472180 | MsG0780036874.01.T01:CDS |
| CTGGCGACAACTACATAACC+TGG | 0.502097 | 7:-15472320 | MsG0780036874.01.T01:CDS |
| GATGCGGAAAGAAAGGTCAT+TGG | 0.503149 | 7:-15471504 | MsG0780036874.01.T01:CDS |
| ATGCGGAAAGAAAGGTCATT+GGG | 0.509313 | 7:-15471503 | MsG0780036874.01.T01:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 7:+15472065 | None:intergenic |
| TGTTATAGATGCGGAAAGAA+AGG | 0.513070 | 7:-15471511 | MsG0780036874.01.T01:CDS |
| GGACTAGACAAGATTCTTGA+GGG | 0.521095 | 7:-15472264 | MsG0780036874.01.T01:CDS |
| TTTGATTGGTCATATTTGAG+AGG | 0.522357 | 7:+15471598 | None:intergenic |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 7:-15471891 | MsG0780036874.01.T01:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 7:+15472066 | None:intergenic |
| CGTGGACGAGGTAGAAATCA+TGG | 0.535808 | 7:-15471673 | MsG0780036874.01.T01:CDS |
| AAAATCAGCAGCTTCAAGAC+AGG | 0.547335 | 7:+15471372 | None:intergenic |
| GTCATTGGGCTAAAACATGC+AGG | 0.564978 | 7:-15471489 | MsG0780036874.01.T01:CDS |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 7:-15472286 | MsG0780036874.01.T01:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 7:-15472285 | MsG0780036874.01.T01:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 7:+15471781 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 7:-15471848 | MsG0780036874.01.T01:CDS |
| ACTATCATCATTATTTCTAG+AGG | 0.581988 | 7:+15471534 | None:intergenic |
| TTCCTGTAGGACGCAACTGG+TGG | 0.593030 | 7:+15471794 | None:intergenic |
| TAAAAGATACAAAGAATCTG+TGG | 0.594054 | 7:-15471449 | MsG0780036874.01.T01:CDS |
| CTATCATCATTATTTCTAGA+GGG | 0.603915 | 7:+15471535 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 7:-15471890 | MsG0780036874.01.T01:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 7:+15471747 | None:intergenic |
| GGGACTAGACAAGATTCTTG+AGG | 0.613207 | 7:-15472265 | MsG0780036874.01.T01:CDS |
| GATGATAGTTGTTATAGATG+CGG | 0.615209 | 7:-15471520 | MsG0780036874.01.T01:CDS |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 7:-15471724 | MsG0780036874.01.T01:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 7:-15471788 | MsG0780036874.01.T01:CDS |
| AAGATACAAAGAATCTGTGG+AGG | 0.669466 | 7:-15471446 | MsG0780036874.01.T01:CDS |
| AATCGCCAAAGAGGACGTAG+TGG | 0.674563 | 7:-15471715 | MsG0780036874.01.T01:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 7:+15472172 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 7:-15472238 | MsG0780036874.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATTAAAGCAAGATTT+GGG | - | Chr7:15471595-15471614 | MsG0780036874.01.T01:CDS | 20.0% |
| !!! | TTCAAAATTTTAAGCATAAC+TGG | - | Chr7:15471366-15471385 | MsG0780036874.01.T01:CDS | 20.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | - | Chr7:15471839-15471858 | MsG0780036874.01.T01:CDS | 25.0% |
| ! | CAATCAAACAAAAATCATCA+GGG | - | Chr7:15472122-15472141 | MsG0780036874.01.T01:CDS | 25.0% |
| ! | GAAGAGGTTATATGAATTAT+AGG | - | Chr7:15472084-15472103 | MsG0780036874.01.T01:CDS | 25.0% |
| ! | GACAAAATTAAAGCAAGATT+TGG | - | Chr7:15471594-15471613 | MsG0780036874.01.T01:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | - | Chr7:15472256-15472275 | MsG0780036874.01.T01:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | - | Chr7:15471723-15471742 | MsG0780036874.01.T01:CDS | 25.0% |
| !!! | ACTATCATCATTATTTCTAG+AGG | + | Chr7:15472174-15472193 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | + | Chr7:15471677-15471696 | None:intergenic | 25.0% |
| !!! | CTATCATCATTATTTCTAGA+GGG | + | Chr7:15472173-15472192 | None:intergenic | 25.0% |
| !!! | TGTTGAAGGAGATTTTAATA+TGG | - | Chr7:15472355-15472374 | MsG0780036874.01.T01:CDS | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | - | Chr7:15471572-15471591 | MsG0780036874.01.T01:CDS | 30.0% | |
| AACAAAAATCATCAGGGTAA+AGG | - | Chr7:15472128-15472147 | MsG0780036874.01.T01:CDS | 30.0% | |
| AGTATTATTGCCCTCATTAA+TGG | - | Chr7:15471629-15471648 | MsG0780036874.01.T01:CDS | 30.0% | |
| CCAATCAAACAAAAATCATC+AGG | - | Chr7:15472121-15472140 | MsG0780036874.01.T01:CDS | 30.0% | |
| GATGATAGTTGTTATAGATG+CGG | - | Chr7:15472185-15472204 | MsG0780036874.01.T01:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | - | Chr7:15471815-15471834 | MsG0780036874.01.T01:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | - | Chr7:15471752-15471771 | MsG0780036874.01.T01:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | - | Chr7:15471814-15471833 | MsG0780036874.01.T01:CDS | 30.0% | |
| TGGAAAAACTATAACTGAAG+TGG | - | Chr7:15471743-15471762 | MsG0780036874.01.T01:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | - | Chr7:15471649-15471668 | MsG0780036874.01.T01:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | + | Chr7:15471642-15471661 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | + | Chr7:15471536-15471555 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | + | Chr7:15471583-15471602 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | + | Chr7:15471582-15471601 | None:intergenic | 30.0% |
| ! | TATCTTTTACACAGATACTC+TGG | + | Chr7:15472245-15472264 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | + | Chr7:15472110-15472129 | None:intergenic | 30.0% |
| ! | TTTTCTTTGCTAATTCCTCT+GGG | + | Chr7:15471485-15471504 | None:intergenic | 30.0% |
| ! | TTTTTCTTTGCTAATTCCTC+TGG | + | Chr7:15471486-15471505 | None:intergenic | 30.0% |
| !! | CCTGATGATTTTTGTTTGAT+TGG | + | Chr7:15472124-15472143 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | - | Chr7:15472146-15472165 | MsG0780036874.01.T01:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | + | Chr7:15471948-15471967 | None:intergenic | 35.0% | |
| AAGATACAAAGAATCTGTGG+AGG | - | Chr7:15472259-15472278 | MsG0780036874.01.T01:CDS | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | - | Chr7:15471419-15471438 | MsG0780036874.01.T01:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | - | Chr7:15471420-15471439 | MsG0780036874.01.T01:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | - | Chr7:15471571-15471590 | MsG0780036874.01.T01:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | + | Chr7:15471643-15471662 | None:intergenic | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | - | Chr7:15472194-15472213 | MsG0780036874.01.T01:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | - | Chr7:15471954-15471973 | MsG0780036874.01.T01:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | - | Chr7:15471955-15471974 | MsG0780036874.01.T01:CDS | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | + | Chr7:15471406-15471425 | None:intergenic | 35.0% |
| AAAATCAGCAGCTTCAAGAC+AGG | + | Chr7:15472336-15472355 | None:intergenic | 40.0% | |
| ACGCCCTTGATTAAAAGTTG+TGG | + | Chr7:15471961-15471980 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | - | Chr7:15471525-15471544 | MsG0780036874.01.T01:CDS | 40.0% | |
| ATGCGGAAAGAAAGGTCATT+GGG | - | Chr7:15472202-15472221 | MsG0780036874.01.T01:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | - | Chr7:15471453-15471472 | MsG0780036874.01.T01:CDS | 40.0% | |
| CAAAGAATCTGTGGAGGAAA+AGG | - | Chr7:15472265-15472284 | MsG0780036874.01.T01:CDS | 40.0% | |
| GGACTAGACAAGATTCTTGA+GGG | - | Chr7:15471441-15471460 | MsG0780036874.01.T01:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | - | Chr7:15472142-15472161 | MsG0780036874.01.T01:CDS | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | - | Chr7:15471788-15471807 | MsG0780036874.01.T01:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | - | Chr7:15471960-15471979 | MsG0780036874.01.T01:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | + | Chr7:15472320-15472339 | None:intergenic | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | - | Chr7:15471785-15471804 | MsG0780036874.01.T01:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | - | Chr7:15471637-15471656 | MsG0780036874.01.T01:CDS | 40.0% |
| !!! | GAAGCTGCTGATTTTGTTGA+AGG | - | Chr7:15472341-15472360 | MsG0780036874.01.T01:CDS | 40.0% |
| GATGCGGAAAGAAAGGTCAT+TGG | - | Chr7:15472201-15472220 | MsG0780036874.01.T01:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | - | Chr7:15471452-15471471 | MsG0780036874.01.T01:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | + | Chr7:15471927-15471946 | None:intergenic | 45.0% | |
| GGGACTAGACAAGATTCTTG+AGG | - | Chr7:15471440-15471459 | MsG0780036874.01.T01:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | - | Chr7:15471981-15472000 | MsG0780036874.01.T01:CDS | 45.0% | |
| GTCATTGGGCTAAAACATGC+AGG | - | Chr7:15472216-15472235 | MsG0780036874.01.T01:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | - | Chr7:15471857-15471876 | MsG0780036874.01.T01:CDS | 45.0% | |
| ! | CATGGTCGAAACCATTTTCG+TGG | - | Chr7:15472050-15472069 | MsG0780036874.01.T01:CDS | 45.0% |
| ! | CGAAACCATTTTCGTGGTAG+AGG | - | Chr7:15472056-15472075 | MsG0780036874.01.T01:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | - | Chr7:15471963-15471982 | MsG0780036874.01.T01:CDS | 45.0% |
| !! | TATTTCTAGAGGGACCTTCC+TGG | + | Chr7:15472163-15472182 | None:intergenic | 45.0% |
| AATCGCCAAAGAGGACGTAG+TGG | - | Chr7:15471990-15472009 | MsG0780036874.01.T01:CDS | 50.0% | |
| CACGACCTCTACCACGAAAA+TGG | + | Chr7:15472064-15472083 | None:intergenic | 50.0% | |
| CATAGCCACTACGTCCTCTT+TGG | + | Chr7:15471998-15472017 | None:intergenic | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | - | Chr7:15472032-15472051 | MsG0780036874.01.T01:CDS | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | - | Chr7:15471385-15471404 | MsG0780036874.01.T01:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | - | Chr7:15471917-15471936 | MsG0780036874.01.T01:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | + | Chr7:15471917-15471936 | None:intergenic | 50.0% | |
| ! | AGTGGCTATGCTCGTTTTGG+TGG | - | Chr7:15472008-15472027 | MsG0780036874.01.T01:CDS | 50.0% |
| ! | CATTTTCGTGGTAGAGGTCG+TGG | - | Chr7:15472062-15472081 | MsG0780036874.01.T01:CDS | 50.0% |
| ! | CGTAGTGGCTATGCTCGTTT+TGG | - | Chr7:15472005-15472024 | MsG0780036874.01.T01:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | - | Chr7:15472014-15472033 | MsG0780036874.01.T01:CDS | 50.0% |
| AACCACCAGTTGCGTCCTAC+AGG | - | Chr7:15471909-15471928 | MsG0780036874.01.T01:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | + | Chr7:15471914-15471933 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | - | Chr7:15471467-15471486 | MsG0780036874.01.T01:CDS | 55.0% |
| CGTGGTAGAGGTCGTGGAAG+AGG | - | Chr7:15472068-15472087 | MsG0780036874.01.T01:CDS | 60.0% | |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | - | Chr7:15472020-15472039 | MsG0780036874.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 15471345 | 15472382 | 15471345 | ID=MsG0780036874.01;Name=MsG0780036874.01 |
| Chr7 | mRNA | 15471345 | 15472382 | 15471345 | ID=MsG0780036874.01.T01;Parent=MsG0780036874.01;Name=MsG0780036874.01.T01;_AED=0.51;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|345 |
| Chr7 | exon | 15471345 | 15472382 | 15471345 | ID=MsG0780036874.01.T01:exon:2677;Parent=MsG0780036874.01.T01 |
| Chr7 | CDS | 15471345 | 15472382 | 15471345 | ID=MsG0780036874.01.T01:cds;Parent=MsG0780036874.01.T01 |
| Gene Sequence |
| Protein sequence |
