AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011125.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011125.01.T01 MTR_2g099140 95.340 515 24 0 1 515 1 515 0.0 1018
MsG0280011125.01.T01 MTR_2g099140 92.817 529 24 1 1 515 1 529 0.0 1010
MsG0280011125.01.T01 MTR_5g015390 32.237 456 247 9 48 481 43 458 9.53e-72 236
MsG0280011125.01.T01 MTR_1g108460 26.200 500 304 15 20 484 53 522 1.16e-39 151
MsG0280011125.01.T01 MTR_2g450550 22.614 482 330 12 30 486 54 517 1.31e-36 142
MsG0280011125.01.T01 MTR_3g106510 23.391 466 307 11 44 480 67 511 1.53e-36 143
MsG0280011125.01.T01 MTR_4g116290 22.863 468 320 8 40 486 41 488 4.26e-34 135
MsG0280011125.01.T01 MTR_2g450550 22.099 362 250 8 149 486 59 412 4.60e-29 119
MsG0280011125.01.T01 MTR_1g108460 27.206 408 230 16 20 392 53 428 2.30e-27 114
MsG0280011125.01.T01 MTR_2g450550 23.077 364 239 11 30 368 54 401 5.19e-24 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011125.01.T01 AT5G67550 26.667 510 337 9 6 497 8 498 5.70e-64 217
MsG0280011125.01.T01 AT2G25270 25.319 470 293 15 44 480 75 519 8.13e-42 157
MsG0280011125.01.T01 AT2G12400 24.899 494 327 10 37 504 63 538 1.35e-41 157
MsG0280011125.01.T01 AT1G71110 23.307 502 334 13 25 498 51 529 1.93e-36 142
MsG0280011125.01.T01 AT1G80540 20.165 486 344 11 28 480 43 517 2.36e-23 103
MsG0280011125.01.T01 AT1G80540 19.958 471 343 10 33 480 12 471 4.43e-23 102
MsG0280011125.01.T01 AT1G80540 19.284 363 265 8 141 480 7 364 1.25e-15 79.3

Find 93 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTTGATTTGAAGGAATT+TGG 0.148694 2:+79872207 None:intergenic
GTATATATATTATATATTAA+AGG 0.199009 2:-79871970 MsG0280011125.01.T01:three_prime_UTR
ACATCACCAACAAGAATTAT+TGG 0.207437 2:-79874587 MsG0280011125.01.T01:CDS
TTGATATTCACTGGAGTATA+TGG 0.251671 2:-79874478 MsG0280011125.01.T01:CDS
TCGGGTTGGTAGGTATAATT+TGG 0.268119 2:+79872772 None:intergenic
TATATATATTATATATTAAA+GGG 0.284972 2:-79871969 MsG0280011125.01.T01:three_prime_UTR
GAGCTTAAGCTGTTGTTATA+AGG 0.299992 2:+79873091 None:intergenic
AAAAGTTATACACGGATTAC+TGG 0.300423 2:+79872071 None:intergenic
ACAGGCTTGTTGTACTTTGC+TGG 0.301195 2:-79873310 MsG0280011125.01.T01:intron
GTATTATGCTGGCTATTCTT+TGG 0.304357 2:-79873278 MsG0280011125.01.T01:CDS
GGCTATTAACATGAGAAATA+TGG 0.305507 2:+79874300 None:intergenic
TTTAACTGCTCTTGATTTGA+AGG 0.331948 2:+79872200 None:intergenic
TTTGTATCCAAACAAGTAAA+AGG 0.335128 2:+79872552 None:intergenic
CCTATTCTTATAGTATTATC+TGG 0.337256 2:+79872742 None:intergenic
AACACAACAGAAGCATTAAA+AGG 0.338281 2:-79874040 MsG0280011125.01.T01:CDS
TGATGCTTTACTAATTCCTT+TGG 0.344919 2:-79874323 MsG0280011125.01.T01:CDS
ATTGAATGTGTATCCAAGTA+TGG 0.345176 2:-79872445 MsG0280011125.01.T01:CDS
ATCTTAGCATATTTCTTCAT+AGG 0.370043 2:+79872357 None:intergenic
TACACTCCAATAATTCTTGT+TGG 0.372190 2:+79874581 None:intergenic
GCAACAGGGCTAGTTCTTGT+AGG 0.391851 2:-79874145 MsG0280011125.01.T01:CDS
TATTTAGTTCATAAGAATAT+AGG 0.395510 2:-79872131 MsG0280011125.01.T01:three_prime_UTR
CAGCTTGTGCTTCAATTTGT+GGG 0.405144 2:+79875650 None:intergenic
AGTATTATCTGGACAATTCT+CGG 0.408061 2:+79872753 None:intergenic
CATACAAAATTCCACATAGA+AGG 0.413531 2:+79874434 None:intergenic
AGTCATGAACATTGTTATCA+TGG 0.416314 2:-79872264 MsG0280011125.01.T01:CDS
GGAGTATATGGATATGCAAT+TGG 0.424393 2:-79874466 MsG0280011125.01.T01:CDS
TTGTAGTCTTTGATATTCAC+TGG 0.425080 2:-79874487 MsG0280011125.01.T01:intron
TCTAACAAATGTTCCATACT+TGG 0.426331 2:+79872432 None:intergenic
CCAGATAATACTATAAGAAT+AGG 0.426578 2:-79872742 MsG0280011125.01.T01:CDS
ATTCAGTATACCTTATTTAG+TGG 0.439839 2:-79872037 MsG0280011125.01.T01:three_prime_UTR
AAGAATATAGGAACCGGTAT+TGG 0.441138 2:-79872119 MsG0280011125.01.T01:three_prime_UTR
ACTTGTTTGGATACAAATGA+TGG 0.450904 2:-79872546 MsG0280011125.01.T01:CDS
CATTGTTATCATGGTTCAGA+TGG 0.453877 2:-79872255 MsG0280011125.01.T01:CDS
TGGGTAGGAATGGTGTTCCT+TGG 0.455580 2:-79872330 MsG0280011125.01.T01:CDS
TATAATAGATACCAAGCCTT+TGG 0.456475 2:+79875693 None:intergenic
GATTTGTGTAGAGTTGCAAC+AGG 0.456871 2:-79874160 MsG0280011125.01.T01:intron
TTTCTGGTGTACTACGGTTA+AGG 0.464706 2:-79873418 MsG0280011125.01.T01:CDS
GGTGTTCCTTGGAGTGATCA+TGG 0.465850 2:-79872319 MsG0280011125.01.T01:CDS
GTTCATAAGAATATAGGAAC+CGG 0.468801 2:-79872125 MsG0280011125.01.T01:three_prime_UTR
ACAGCTTGTGCTTCAATTTG+TGG 0.469456 2:+79875649 None:intergenic
GCACTAAATTCAGATAGAAC+TGG 0.472426 2:+79873034 None:intergenic
TATGAAGAAATATGCTAAGA+TGG 0.477004 2:-79872355 MsG0280011125.01.T01:CDS
TCACCTGGTGATTCTGCTGC+AGG 0.478707 2:-79875625 MsG0280011125.01.T01:intron
TAATTTGGTGGCGCTGAGAA+AGG 0.479612 2:+79872787 None:intergenic
TTCAGTATACCTTATTTAGT+GGG 0.484635 2:-79872036 MsG0280011125.01.T01:three_prime_UTR
GTATTATCTGGACAATTCTC+GGG 0.490684 2:+79872754 None:intergenic
AGGCATGGAGAGTAACTTGA+TGG 0.496619 2:-79874020 MsG0280011125.01.T01:CDS
GTTCTATCTGAATTTAGTGC+TGG 0.497245 2:-79873031 MsG0280011125.01.T01:CDS
CAACAGGGCTAGTTCTTGTA+GGG 0.506049 2:-79874144 MsG0280011125.01.T01:CDS
GGTTGTTGACAGTATTATGC+TGG 0.511614 2:-79873289 MsG0280011125.01.T01:CDS
TGAGCAATTCTTGACATGGA+AGG 0.514152 2:+79873065 None:intergenic
TATCTATAATCTTGTTAATG+AGG 0.517870 2:-79873008 MsG0280011125.01.T01:intron
TACTTGGATACACATTCAAT+AGG 0.517880 2:+79872448 None:intergenic
AGAAATATGCTAAGATGGCA+TGG 0.519247 2:-79872350 MsG0280011125.01.T01:CDS
AATAGGCGCCTTATCAGTAA+AGG 0.523635 2:-79873902 MsG0280011125.01.T01:CDS
GGTTGGTAGGTATAATTTGG+TGG 0.528341 2:+79872775 None:intergenic
GTCAGAACAGATTACACAAA+GGG 0.530197 2:-79874667 MsG0280011125.01.T01:CDS
CATAATCAAAACAGCAAACA+AGG 0.542949 2:-79874080 MsG0280011125.01.T01:CDS
AGAAGCCTCAACATTAACAT+TGG 0.547079 2:+79873994 None:intergenic
GAAGCACAAGCTGTCTCACC+TGG 0.551867 2:-79875640 MsG0280011125.01.T01:CDS
CTTGTTTGGATACAAATGAT+GGG 0.553416 2:-79872545 MsG0280011125.01.T01:CDS
ATTTCTTCATAGGCTTGCAA+TGG 0.560254 2:+79872367 None:intergenic
AACAGAAGCATTAAAAGGCA+TGG 0.560327 2:-79874035 MsG0280011125.01.T01:CDS
TTAAAGATGTTCATAGCCAA+AGG 0.561779 2:-79875709 None:intergenic
GAACAGTTTCTGGTGTACTA+CGG 0.561972 2:-79873424 MsG0280011125.01.T01:intron
GATGTTCATAGCCAAAGGCT+TGG 0.569407 2:-79875704 MsG0280011125.01.T01:CDS
TATATACAAATGTTGCATGT+CGG 0.581404 2:+79871986 None:intergenic
TATCTGGACAATTCTCGGGT+TGG 0.582340 2:+79872758 None:intergenic
CTGGTGTACTACGGTTAAGG+AGG 0.583051 2:-79873415 MsG0280011125.01.T01:CDS
TGGACAATTCTCGGGTTGGT+AGG 0.583599 2:+79872762 None:intergenic
TCACAACTCTTGTAATTACA+AGG 0.584647 2:+79874343 None:intergenic
ATATATATTATATATTAAAG+GGG 0.586177 2:-79871968 MsG0280011125.01.T01:three_prime_UTR
ATATAGGAACCGGTATTGGA+AGG 0.587145 2:-79872115 MsG0280011125.01.T01:three_prime_UTR
TCATGGTTCAGATGGTTCTG+TGG 0.587485 2:-79872247 MsG0280011125.01.T01:CDS
AAGGGATAATACAATTAGAG+TGG 0.592143 2:-79874649 MsG0280011125.01.T01:CDS
GAAATATGCTAAGATGGCAT+GGG 0.595556 2:-79872349 MsG0280011125.01.T01:CDS
AACATGAGAAATATGGCCAA+AGG 0.602955 2:+79874307 None:intergenic
GCTTTGAGCAATTCTTGACA+TGG 0.606847 2:+79873061 None:intergenic
GAATTTGGTACTTCACAATG+AGG 0.631175 2:+79872222 None:intergenic
TGGTGTACTACGGTTAAGGA+GGG 0.631651 2:-79873414 MsG0280011125.01.T01:CDS
TATGCTAAGATGGCATGGGT+AGG 0.632516 2:-79872345 MsG0280011125.01.T01:CDS
TATATATTATATATTAAAGG+GGG 0.635375 2:-79871967 MsG0280011125.01.T01:three_prime_UTR
AAGGATTGCAAACAAGAACA+AGG 0.637380 2:+79872806 None:intergenic
AATGATGGGAGTTGTGACAA+TGG 0.638209 2:-79872531 MsG0280011125.01.T01:CDS
ACAAAACCATGATCACTCCA+AGG 0.642179 2:+79872313 None:intergenic
TAGGCTTGCAATGGTCAACA+AGG 0.643609 2:+79872376 None:intergenic
TAAGATGGCATGGGTAGGAA+TGG 0.645876 2:-79872340 MsG0280011125.01.T01:CDS
GGAATGTTATGCCTTCTATG+TGG 0.645924 2:-79874445 MsG0280011125.01.T01:CDS
AAGAATAGGTGACATTCCAA+AGG 0.648365 2:-79872728 MsG0280011125.01.T01:intron
GGAAGCCAATGTTAATGTTG+AGG 0.652341 2:-79873999 MsG0280011125.01.T01:CDS
TTACCTGCAGCAGAATCACC+AGG 0.670804 2:+79875622 None:intergenic
ATTTGTGTAGAGTTGCAACA+GGG 0.687136 2:-79874159 MsG0280011125.01.T01:intron
TGTCAGAACAGATTACACAA+AGG 0.693803 2:-79874668 MsG0280011125.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATTATATATTAAA+GGG - Chr2:79875688-79875707 MsG0280011125.01.T01:CDS 0.0%
!! AAAATATTACTATGTAATTA+TGG + Chr2:79874451-79874470 None:intergenic 10.0%
!! TATATATTATATATTAAAGG+GGG - Chr2:79875690-79875709 MsG0280011125.01.T01:CDS 10.0%
!!! TTGATATTTTTATATTTGTT+TGG + Chr2:79872834-79872853 None:intergenic 10.0%
!! CATTTAAAAAAAGTTATACA+CGG + Chr2:79875597-79875616 None:intergenic 15.0%
!!! CATTTTTATGATATTTGATT+GGG + Chr2:79872408-79872427 None:intergenic 15.0%
!!! GTAATATTTTTATAAAGTAG+AGG - Chr2:79874461-79874480 MsG0280011125.01.T01:CDS 15.0%
!!! TACTTTTTGAAATTATCTAA+TGG + Chr2:79873036-79873055 None:intergenic 15.0%
!!! TATTTAGTTCATAAGAATAT+AGG - Chr2:79875526-79875545 MsG0280011125.01.T01:intron 15.0%
!! AAAATGAATGAAAAATTGCA+TGG - Chr2:79874045-79874064 MsG0280011125.01.T01:CDS 20.0%
!! AAATCATAAAAATGGTCAAA+TGG - Chr2:79872454-79872473 MsG0280011125.01.T01:CDS 20.0%
!! AATCATAAAAATGGTCAAAT+GGG - Chr2:79872455-79872474 MsG0280011125.01.T01:CDS 20.0%
!! ACATGATATATTTATTAACC+AGG - Chr2:79872534-79872553 MsG0280011125.01.T01:CDS 20.0%
!! ACATTAATATAGAACTCATT+TGG + Chr2:79874714-79874733 None:intergenic 20.0%
!! ATTCATGTTGAAATATTACA+TGG - Chr2:79872167-79872186 MsG0280011125.01.T01:exon 20.0%
!! CCAATCAAATATCATAAAAA+TGG - Chr2:79872406-79872425 MsG0280011125.01.T01:CDS 20.0%
!! TATATAGGACTAAAGTTAAA+TGG - Chr2:79875005-79875024 MsG0280011125.01.T01:intron 20.0%
!! TATCTATAATCTTGTTAATG+AGG - Chr2:79874649-79874668 MsG0280011125.01.T01:CDS 20.0%
!! TGTAATTATGGTAATAAGAA+AGG + Chr2:79874439-79874458 None:intergenic 20.0%
!! TTTACTATTTAAACAGTAGT+AGG + Chr2:79874028-79874047 None:intergenic 20.0%
!! TTTCGTCATTAAAATTGTAA+TGG - Chr2:79873809-79873828 MsG0280011125.01.T01:intron 20.0%
!!! AAATTTTATCCCACTAAATA+AGG + Chr2:79875633-79875652 None:intergenic 20.0%
!!! AATGTGATCATTCTTTTTTA+AGG - Chr2:79875074-79875093 MsG0280011125.01.T01:intron 20.0%
!!! ATGTTAGAAATTGTTTTGTT+AGG - Chr2:79872736-79872755 MsG0280011125.01.T01:CDS 20.0%
!!! CCATTTTTATGATATTTGAT+TGG + Chr2:79872409-79872428 None:intergenic 20.0%
!!! TCATTTTTTGAAATTGTCTT+TGG - Chr2:79872921-79872940 MsG0280011125.01.T01:intron 20.0%
!!! TGTTAGAAATTGTTTTGTTA+GGG - Chr2:79872737-79872756 MsG0280011125.01.T01:CDS 20.0%
!!! TGTTCATAAACTATTGTTTT+TGG - Chr2:79874731-79874750 MsG0280011125.01.T01:intron 20.0%
!!! TTAACATTGTGTTTTGATTT+TGG - Chr2:79874156-79874175 MsG0280011125.01.T01:intron 20.0%
! AAGGTTTATCATCACAAAAA+TGG - Chr2:79873266-79873285 MsG0280011125.01.T01:CDS 25.0%
! AATGGAATAAATTTCATTGC+AGG - Chr2:79874072-79874091 MsG0280011125.01.T01:CDS 25.0%
! AATTTCAACGACTAAATTGA+CGG - Chr2:79873954-79873973 MsG0280011125.01.T01:CDS 25.0%
! ACATGATTCTCATATAATGT+TGG - Chr2:79874200-79874219 MsG0280011125.01.T01:intron 25.0%
! ATCACATTGTTAATACATCT+TGG + Chr2:79875062-79875081 None:intergenic 25.0%
! ATCTTAGCATATTTCTTCAT+AGG + Chr2:79875303-79875322 None:intergenic 25.0%
! CCAGATAATACTATAAGAAT+AGG - Chr2:79874915-79874934 MsG0280011125.01.T01:intron 25.0%
! CCTATTCTTATAGTATTATC+TGG + Chr2:79874918-79874937 None:intergenic 25.0%
! CTCTAATGATTAATCACTAA+AGG + Chr2:79873410-79873429 None:intergenic 25.0%
! GTCATTAAAATTGTAATGGT+CGG - Chr2:79873813-79873832 MsG0280011125.01.T01:intron 25.0%
! TACTCCAACATGAAATTAAT+AGG + Chr2:79874772-79874791 None:intergenic 25.0%
! TATATACAAATGTTGCATGT+CGG + Chr2:79875674-79875693 None:intergenic 25.0%
! TATGAAGAAATATGCTAAGA+TGG - Chr2:79875302-79875321 MsG0280011125.01.T01:intron 25.0%
! TGTATCAATCATTATGTGTA+TGG + Chr2:79874183-79874202 None:intergenic 25.0%
! TTGAATATAATCACATGTGT+CGG - Chr2:79872278-79872297 MsG0280011125.01.T01:CDS 25.0%
! TTTAGTCCTTGAAATTGAAA+AGG + Chr2:79873981-79874000 None:intergenic 25.0%
! TTTGTATCCAAACAAGTAAA+AGG + Chr2:79875108-79875127 None:intergenic 25.0%
!! AATTTTCATTGAACAGTTTC+TGG - Chr2:79874223-79874242 MsG0280011125.01.T01:intron 25.0%
!! AGAATGCATCTTTTACAATT+TGG + Chr2:79875254-79875273 None:intergenic 25.0%
!! ATTAAAGCCTTTTACTTGTT+TGG - Chr2:79875098-79875117 MsG0280011125.01.T01:intron 25.0%
!! ATTCAGTATACCTTATTTAG+TGG - Chr2:79875620-79875639 MsG0280011125.01.T01:intron 25.0%
!! GATAATTTCAAAAAGTACAG+AGG - Chr2:79873039-79873058 MsG0280011125.01.T01:CDS 25.0%
!! TTCAGTATACCTTATTTAGT+GGG - Chr2:79875621-79875640 MsG0280011125.01.T01:intron 25.0%
!!! AACTTTTTTGGATTTTTGGT+TGG - Chr2:79874791-79874810 MsG0280011125.01.T01:intron 25.0%
!!! ACATCTTGGTCTTATAATTT+AGG + Chr2:79875048-79875067 None:intergenic 25.0%
!!! AGTTATTTTGCTAATCACAA+AGG - Chr2:79873247-79873266 MsG0280011125.01.T01:intron 25.0%
!!! CGACACATACAATTTTTTTA+TGG - Chr2:79873442-79873461 MsG0280011125.01.T01:intron 25.0%
!!! GAGTAACTTTTTTGGATTTT+TGG - Chr2:79874787-79874806 MsG0280011125.01.T01:intron 25.0%
!!! GATTATGTTTTGGACTATAA+AGG - Chr2:79875368-79875387 MsG0280011125.01.T01:intron 25.0%
!!! TGGTTTTGTTGATTATGTTT+TGG - Chr2:79875358-79875377 MsG0280011125.01.T01:intron 25.0%
!!! TGTAATTTTAGCATACTTAC+AGG + Chr2:79874276-79874295 None:intergenic 25.0%
AAATGCAAGCTCAGAAAAAT+AGG - Chr2:79873738-79873757 MsG0280011125.01.T01:intron 30.0%
AAGGGATAATACAATTAGAG+TGG - Chr2:79873008-79873027 MsG0280011125.01.T01:intron 30.0%
AATTAATAGGTCCAGTATGT+TGG + Chr2:79874759-79874778 None:intergenic 30.0%
ACATCACCAACAAGAATTAT+TGG - Chr2:79873070-79873089 MsG0280011125.01.T01:CDS 30.0%
ACGTCTTCAATTAGAAATGT+CGG - Chr2:79872327-79872346 MsG0280011125.01.T01:CDS 30.0%
ACTTGTTTGGATACAAATGA+TGG - Chr2:79875111-79875130 MsG0280011125.01.T01:intron 30.0%
AGTATTATCTGGACAATTCT+CGG + Chr2:79874907-79874926 None:intergenic 30.0%
AGTCATGAACATTGTTATCA+TGG - Chr2:79875393-79875412 MsG0280011125.01.T01:intron 30.0%
ATTGAATGTGTATCCAAGTA+TGG - Chr2:79875212-79875231 MsG0280011125.01.T01:intron 30.0%
ATTTATTAACCAGGAAGAGT+AGG - Chr2:79872543-79872562 MsG0280011125.01.T01:CDS 30.0%
CATAATCAAAACAGCAAACA+AGG - Chr2:79873577-79873596 MsG0280011125.01.T01:intron 30.0%
CATACAAAATTCCACATAGA+AGG + Chr2:79873226-79873245 None:intergenic 30.0%
CTTGTTTGGATACAAATGAT+GGG - Chr2:79875112-79875131 MsG0280011125.01.T01:intron 30.0%
GAAAAATTGCATGGAAGTAA+TGG - Chr2:79874054-79874073 MsG0280011125.01.T01:CDS 30.0%
GAAGTTAGACTCAAAAAAGA+GGG + Chr2:79873113-79873132 None:intergenic 30.0%
GGCTATTAACATGAGAAATA+TGG + Chr2:79873360-79873379 None:intergenic 30.0%
GTAGATTGCAGAAAATTTGA+AGG + Chr2:79872118-79872137 None:intergenic 30.0%
GTATGTTAGTAGCTAAACTT+GGG + Chr2:79872716-79872735 None:intergenic 30.0%
GTTCATAAGAATATAGGAAC+CGG - Chr2:79875532-79875551 MsG0280011125.01.T01:intron 30.0%
GTTTATCATCACAAAAATGG+AGG - Chr2:79873269-79873288 MsG0280011125.01.T01:CDS 30.0%
GTTTCTCAAACTTGTGAAAT+TGG - Chr2:79873844-79873863 MsG0280011125.01.T01:intron 30.0%
TACACTCCAATAATTCTTGT+TGG + Chr2:79873079-79873098 None:intergenic 30.0%
TACTTGGATACACATTCAAT+AGG + Chr2:79875212-79875231 None:intergenic 30.0%
TATAATAGATACCAAGCCTT+TGG + Chr2:79871967-79871986 None:intergenic 30.0%
TCACAACTCTTGTAATTACA+AGG + Chr2:79873317-79873336 None:intergenic 30.0%
TCTAACAAATGTTCCATACT+TGG + Chr2:79875228-79875247 None:intergenic 30.0%
TGAAGTTAGACTCAAAAAAG+AGG + Chr2:79873114-79873133 None:intergenic 30.0%
TGATGCTTTACTAATTCCTT+TGG - Chr2:79873334-79873353 MsG0280011125.01.T01:intron 30.0%
TGGACCTATTAATTTCATGT+TGG - Chr2:79874765-79874784 MsG0280011125.01.T01:intron 30.0%
TGTATGTTAGTAGCTAAACT+TGG + Chr2:79872717-79872736 None:intergenic 30.0%
TTAGTCCTATATAAACAGCT+CGG + Chr2:79874998-79875017 None:intergenic 30.0%
TTGATATTCACTGGAGTATA+TGG - Chr2:79873179-79873198 MsG0280011125.01.T01:intron 30.0%
TTGTAGTCTTTGATATTCAC+TGG - Chr2:79873170-79873189 MsG0280011125.01.T01:intron 30.0%
TTTATTAACCAGGAAGAGTA+GGG - Chr2:79872544-79872563 MsG0280011125.01.T01:CDS 30.0%
! AAAAGTTATACACGGATTAC+TGG + Chr2:79875589-79875608 None:intergenic 30.0%
! AAATTTTGCACCACGATTTA+AGG + Chr2:79873879-79873898 None:intergenic 30.0%
! AATTTCAAAAAGTACAGAGG+AGG - Chr2:79873042-79873061 MsG0280011125.01.T01:CDS 30.0%
! AATTTTGCACCACGATTTAA+GGG + Chr2:79873878-79873897 None:intergenic 30.0%
! ATTTTCACTCATTATGAGAG+AGG + Chr2:79872769-79872788 None:intergenic 30.0%
! CTTCTATGTGGAATTTTGTA+TGG - Chr2:79873224-79873243 MsG0280011125.01.T01:intron 30.0%
! TTTAACTGCTCTTGATTTGA+AGG + Chr2:79875460-79875479 None:intergenic 30.0%
!! AACACAACAGAAGCATTAAA+AGG - Chr2:79873617-79873636 MsG0280011125.01.T01:intron 30.0%
!! TAATTTGTTATGCTTTAGCC+AGG + Chr2:79872580-79872599 None:intergenic 30.0%
!! TTTTCAAGCCTTTACTGATA+AGG + Chr2:79873766-79873785 None:intergenic 30.0%
!!! GAGAGACAATCTTTTTTTCA+TGG + Chr2:79872662-79872681 None:intergenic 30.0%
!!! TCATGTTGGAGTAACTTTTT+TGG - Chr2:79874779-79874798 MsG0280011125.01.T01:intron 30.0%
AACATGAGAAATATGGCCAA+AGG + Chr2:79873353-79873372 None:intergenic 35.0%
AAGAATAGGTGACATTCCAA+AGG - Chr2:79874929-79874948 MsG0280011125.01.T01:intron 35.0%
AAGAATATAGGAACCGGTAT+TGG - Chr2:79875538-79875557 MsG0280011125.01.T01:intron 35.0%
AAGGATTGCAAACAAGAACA+AGG + Chr2:79874854-79874873 None:intergenic 35.0%
ACAATCCGAGCTGTTTATAT+AGG - Chr2:79874990-79875009 MsG0280011125.01.T01:intron 35.0%
ACTCTTCTATCGTATACCTT+TGG + Chr2:79874948-79874967 None:intergenic 35.0%
AGAAATATGCTAAGATGGCA+TGG - Chr2:79875307-79875326 MsG0280011125.01.T01:intron 35.0%
AGAAGCCTCAACATTAACAT+TGG + Chr2:79873666-79873685 None:intergenic 35.0%
ATAAACAGCTCGGATTGTAT+AGG + Chr2:79874988-79875007 None:intergenic 35.0%
ATTTCTTCATAGGCTTGCAA+TGG + Chr2:79875293-79875312 None:intergenic 35.0%
ATTTGTGTAGAGTTGCAACA+GGG - Chr2:79873498-79873517 MsG0280011125.01.T01:intron 35.0%
CATTGTTATCATGGTTCAGA+TGG - Chr2:79875402-79875421 MsG0280011125.01.T01:intron 35.0%
GAAATATGCTAAGATGGCAT+GGG - Chr2:79875308-79875327 MsG0280011125.01.T01:intron 35.0%
GAATTTAGTCCCTTAAATCG+TGG - Chr2:79873866-79873885 MsG0280011125.01.T01:intron 35.0%
GAATTTGGTACTTCACAATG+AGG + Chr2:79875438-79875457 None:intergenic 35.0%
GAGCTTAAGCTGTTGTTATA+AGG + Chr2:79874569-79874588 None:intergenic 35.0%
GATTAATCACTAAAGGCTAG+AGG + Chr2:79873403-79873422 None:intergenic 35.0%
GCACTAAATTCAGATAGAAC+TGG + Chr2:79874626-79874645 None:intergenic 35.0%
GGAGTATATGGATATGCAAT+TGG - Chr2:79873191-79873210 MsG0280011125.01.T01:intron 35.0%
GTATTATCTGGACAATTCTC+GGG + Chr2:79874906-79874925 None:intergenic 35.0%
GTCAGAACAGATTACACAAA+GGG - Chr2:79872990-79873009 MsG0280011125.01.T01:intron 35.0%
GTCGTTGAAATTTGCGAAAT+AGG + Chr2:79873946-79873965 None:intergenic 35.0%
GTTCTATCTGAATTTAGTGC+TGG - Chr2:79874626-79874645 MsG0280011125.01.T01:CDS 35.0%
TAAACAGCTCGGATTGTATA+GGG + Chr2:79874987-79875006 None:intergenic 35.0%
TCATGCATATACATCACTTG+AGG + Chr2:79873472-79873491 None:intergenic 35.0%
TGTCAGAACAGATTACACAA+AGG - Chr2:79872989-79873008 MsG0280011125.01.T01:intron 35.0%
! GCTCTTGATTTGAAGGAATT+TGG + Chr2:79875453-79875472 None:intergenic 35.0%
! GTATTATGCTGGCTATTCTT+TGG - Chr2:79874379-79874398 MsG0280011125.01.T01:CDS 35.0%
!! AACAGAAGCATTAAAAGGCA+TGG - Chr2:79873622-79873641 MsG0280011125.01.T01:intron 35.0%
!! CTTGCTGTAACAATTTTGTC+AGG - Chr2:79874131-79874150 MsG0280011125.01.T01:CDS 35.0%
!! CTTTTGCTACTCTTTTTCAC+AGG - Chr2:79874329-79874348 MsG0280011125.01.T01:CDS 35.0%
!!! CTGAAGTTTTAGCTTCTGAA+TGG + Chr2:79873550-79873569 None:intergenic 35.0%
!!! TGTTTTTGGATCCAACATAC+TGG - Chr2:79874745-79874764 MsG0280011125.01.T01:intron 35.0%
AATAGGCGCCTTATCAGTAA+AGG - Chr2:79873755-79873774 MsG0280011125.01.T01:intron 40.0%
ACAAAACCATGATCACTCCA+AGG + Chr2:79875347-79875366 None:intergenic 40.0%
ACAGCTTGTGCTTCAATTTG+TGG + Chr2:79872011-79872030 None:intergenic 40.0%
ACTCTGATAGACACATACAC+CGG - Chr2:79872201-79872220 MsG0280011125.01.T01:CDS 40.0%
CAGCTTGTGCTTCAATTTGT+GGG + Chr2:79872010-79872029 None:intergenic 40.0%
GATTTGTGTAGAGTTGCAAC+AGG - Chr2:79873497-79873516 MsG0280011125.01.T01:intron 40.0%
GCAAGGTTGAATGACTTACA+TGG + Chr2:79873381-79873400 None:intergenic 40.0%
GGAAGCCAATGTTAATGTTG+AGG - Chr2:79873658-79873677 MsG0280011125.01.T01:intron 40.0%
GGAATGTTATGCCTTCTATG+TGG - Chr2:79873212-79873231 MsG0280011125.01.T01:intron 40.0%
GGCAACACACATTATCATGT+CGG - Chr2:79872236-79872255 MsG0280011125.01.T01:CDS 40.0%
GGTTGTTGACAGTATTATGC+TGG - Chr2:79874368-79874387 MsG0280011125.01.T01:CDS 40.0%
GTTAGTAGCTAAACTTGGGT+AGG + Chr2:79872712-79872731 None:intergenic 40.0%
TGAGCAATTCTTGACATGGA+AGG + Chr2:79874595-79874614 None:intergenic 40.0%
TGTAGTCTGTAGAGACTATG+AGG + Chr2:79872072-79872091 None:intergenic 40.0%
TGTTGCCAAAATGATGTGTC+CGG + Chr2:79872223-79872242 None:intergenic 40.0%
! AATGATGGGAGTTGTGACAA+TGG - Chr2:79875126-79875145 MsG0280011125.01.T01:intron 40.0%
! ATACACCGGACACATCATTT+TGG - Chr2:79872215-79872234 MsG0280011125.01.T01:CDS 40.0%
! ATATAGGAACCGGTATTGGA+AGG - Chr2:79875542-79875561 MsG0280011125.01.T01:intron 40.0%
! GACGGACCTTTTCAATTTCA+AGG - Chr2:79873972-79873991 MsG0280011125.01.T01:CDS 40.0%
! GCATTTTTGCCTTCCAATAC+CGG + Chr2:79875554-79875573 None:intergenic 40.0%
! GCTTTGAGCAATTCTTGACA+TGG + Chr2:79874599-79874618 None:intergenic 40.0%
! TTTCTGGTGTACTACGGTTA+AGG - Chr2:79874239-79874258 MsG0280011125.01.T01:intron 40.0%
!! GAACAGTTTCTGGTGTACTA+CGG - Chr2:79874233-79874252 MsG0280011125.01.T01:intron 40.0%
!! GGTTGGTAGGTATAATTTGG+TGG + Chr2:79874885-79874904 None:intergenic 40.0%
!! TCGGGTTGGTAGGTATAATT+TGG + Chr2:79874888-79874907 None:intergenic 40.0%
!!! GGATTTTTGGTTGGTTGTCT+AGG - Chr2:79874800-79874819 MsG0280011125.01.T01:intron 40.0%
AGGCATGGAGAGTAACTTGA+TGG - Chr2:79873637-79873656 MsG0280011125.01.T01:intron 45.0%
ATCACTAAAGGCTAGAGGCA+AGG + Chr2:79873398-79873417 None:intergenic 45.0%
GGAGAGACAGTAGAGCAAAT+AGG + Chr2:79874007-79874026 None:intergenic 45.0%
TAAGATGGCATGGGTAGGAA+TGG - Chr2:79875317-79875336 MsG0280011125.01.T01:intron 45.0%
TAGGCTTGCAATGGTCAACA+AGG + Chr2:79875284-79875303 None:intergenic 45.0%
TATCTGGACAATTCTCGGGT+TGG + Chr2:79874902-79874921 None:intergenic 45.0%
TATGCTAAGATGGCATGGGT+AGG - Chr2:79875312-79875331 MsG0280011125.01.T01:intron 45.0%
TGGTGTACTACGGTTAAGGA+GGG - Chr2:79874243-79874262 MsG0280011125.01.T01:intron 45.0%
! TCATGGTTCAGATGGTTCTG+TGG - Chr2:79875410-79875429 MsG0280011125.01.T01:intron 45.0%
!! ACAGGCTTGTTGTACTTTGC+TGG - Chr2:79874347-79874366 MsG0280011125.01.T01:CDS 45.0%
!! CAACAGGGCTAGTTCTTGTA+GGG - Chr2:79873513-79873532 MsG0280011125.01.T01:intron 45.0%
!! TAATTTGGTGGCGCTGAGAA+AGG + Chr2:79874873-79874892 None:intergenic 45.0%
!! AAAAAATAAAATCATAAAAA+TGG - Chr2:79872446-79872465 MsG0280011125.01.T01:CDS 5.0%
!! ATATATATTATATATTAAAG+GGG - Chr2:79875689-79875708 MsG0280011125.01.T01:CDS 5.0%
!! GTATATATATTATATATTAA+AGG - Chr2:79875687-79875706 MsG0280011125.01.T01:CDS 5.0%
!! TAATTAAATATTTATTTCAA+AGG - Chr2:79872478-79872497 MsG0280011125.01.T01:CDS 5.0%
AACCAGGAAGAGTAGGGCTA+AGG - Chr2:79872550-79872569 MsG0280011125.01.T01:CDS 50.0%
CTGGTGTACTACGGTTAAGG+AGG - Chr2:79874242-79874261 MsG0280011125.01.T01:intron 50.0%
GAGTAGGGCTAAGGAATTCC+TGG - Chr2:79872559-79872578 MsG0280011125.01.T01:CDS 50.0%
GGTGTTCCTTGGAGTGATCA+TGG - Chr2:79875338-79875357 MsG0280011125.01.T01:intron 50.0%
TGGGTAGGAATGGTGTTCCT+TGG - Chr2:79875327-79875346 MsG0280011125.01.T01:intron 50.0%
TTACCTGCAGCAGAATCACC+AGG + Chr2:79872038-79872057 None:intergenic 50.0%
TTCCTTAGCCCTACTCTTCC+TGG + Chr2:79872555-79872574 None:intergenic 50.0%
!! GCAACAGGGCTAGTTCTTGT+AGG - Chr2:79873512-79873531 MsG0280011125.01.T01:intron 50.0%
!! TGGACAATTCTCGGGTTGGT+AGG + Chr2:79874898-79874917 None:intergenic 50.0%
GAAGCACAAGCTGTCTCACC+TGG - Chr2:79872017-79872036 MsG0280011125.01.T01:three_prime_UTR 55.0%
TCACCTGGTGATTCTGCTGC+AGG - Chr2:79872032-79872051 MsG0280011125.01.T01:three_prime_UTR 55.0%
!! CACATGTGTCGGTGTCACGT+CGG - Chr2:79872289-79872308 MsG0280011125.01.T01:CDS 55.0%
!! TGTCGGTGTCACGTCGGAGT+CGG - Chr2:79872295-79872314 MsG0280011125.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 79871954 79875725 79871954 ID=MsG0280011125.01;Name=MsG0280011125.01
Chr2 mRNA 79871954 79875725 79871954 ID=MsG0280011125.01.T01;Parent=MsG0280011125.01;Name=MsG0280011125.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0.77|0.7|1|0.77|0.7|10|231|515
Chr2 exon 79871954 79872583 79871954 ID=MsG0280011125.01.T01:exon:17593;Parent=MsG0280011125.01.T01
Chr2 exon 79872729 79872857 79872729 ID=MsG0280011125.01.T01:exon:17592;Parent=MsG0280011125.01.T01
Chr2 exon 79873009 79873159 79873009 ID=MsG0280011125.01.T01:exon:17591;Parent=MsG0280011125.01.T01
Chr2 exon 79873257 79873328 79873257 ID=MsG0280011125.01.T01:exon:17590;Parent=MsG0280011125.01.T01
Chr2 exon 79873404 79873440 79873404 ID=MsG0280011125.01.T01:exon:17589;Parent=MsG0280011125.01.T01
Chr2 exon 79873527 79873585 79873527 ID=MsG0280011125.01.T01:exon:17588;Parent=MsG0280011125.01.T01
Chr2 exon 79873890 79874171 79873890 ID=MsG0280011125.01.T01:exon:17587;Parent=MsG0280011125.01.T01
Chr2 exon 79874298 79874503 79874298 ID=MsG0280011125.01.T01:exon:17586;Parent=MsG0280011125.01.T01
Chr2 exon 79874584 79874696 79874584 ID=MsG0280011125.01.T01:exon:17585;Parent=MsG0280011125.01.T01
Chr2 exon 79875626 79875725 79875626 ID=MsG0280011125.01.T01:exon:17584;Parent=MsG0280011125.01.T01
Chr2 CDS 79875626 79875725 79875626 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79874584 79874696 79874584 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79874298 79874503 79874298 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79873890 79874171 79873890 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79873527 79873585 79873527 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79873404 79873440 79873404 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79873257 79873328 79873257 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79873009 79873159 79873009 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79872729 79872857 79872729 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 CDS 79872185 79872583 79872185 ID=MsG0280011125.01.T01:cds;Parent=MsG0280011125.01.T01
Chr2 three_prime_UTR 79871954 79872184 79871954 ID=MsG0280011125.01.T01:three_prime_utr;Parent=MsG0280011125.01.T01
Gene Sequence

>MsG0280011125.01.T01

ATGTTCATAGCCAAAGGCTTGGTATCTATTATAGTGATTATAACATTGATTCTCCCACAAATTGAAGCACAAGCTGTCTCACCTGGTGATTCTGCTGCAGATAATGTGTCAGAACAGATTACACAAAGGGATAATACAATTAGAGTGGATCCATTAGATAATTTCAAAAAGTACAGAGGAGGATTTGACATCACCAACAAGAATTATTGGAGTTCTTTGATATTCACTGGAGTATATGGATATGCAATTGGAATGTTATGCCTTCTATGTGGAATTTTGTATGGAGTTATTTTGCTAATCACAAAGGTTTATCATCACAAAAATGGAGGAAAAAGAATGAAGAAAGTGTTTCCTTGTAATTACAAGAGTTGTGATGCTTTACTAATTCCTTTGGCCATATTTCTCATGTTAATAGCCATAGTTGCAACAGGGCTAGTTCTTGTAGGGAGTGCAAGATTCCATTCAGAAGCTAAAACTTCAGTTGATATCATAATCAAAACAGCAAACAAGGCATCAGAAACAATACACAACACAACAGAAGCATTAAAAGGCATGGAGAGTAACTTGATGGAAGCCAATGTTAATGTTGAGGCTTCTTCAAATCTTGACTCTACAGCTGAAAAACTTGATGATGCATCTGTAAATATTGAAATGCAAGCTCAGAAAAATAGGCGCCTTATCAGTAAAGGCTTGAAAATAGTGTTTATAACTACTACAGTGATCATGTGCTTGAACTTGCTTGCTGTAACAATTTTGTCAGTTTCTGGTGTACTACGGTTAAGGAGGGCATTGTACCTGCTTGTTGTACTTTGCTGGTTGTTGACAGTATTATGCTGGCTATTCTTTGGAGTCTATTTCTTCTTAGAAAAATTTTCTAGTGATGCTTGCACAGCTCTTGACAACTTTCAAGAAAATCCTTATAACAACAGCTTAAGCTCAATCCTTCCATGTCAAGAATTGCTCAAAGCAAAACCAGTTCTATCTGAATTTAGTGCTGGTATCTATAATCTTGTTAATGAGGTGAATGCAAACATATCAATGCAGACAAACCTTGTTCTTGTTTGCAATCCTTTCTCAGCGCCACCAAATTATACCTACCAACCCGAGAATTGTCCAGATAATACTATAAGAATAGGTGACATTCCAAAGGTATTAAAGCCTTTTACTTGTTTGGATACAAATGATGGGAGTTGTGACAATGGAAATTTCATAACAAACAGTGAATATGCAAGAGTTGAAACTTACACAAATTCAATTCAAGACCTATTGAATGTGTATCCAAGTATGGAACATTTGTTAGAATGCCAAATTGTAAAAGATGCATTCTCTCAAGTCCTTGTTGACCATTGCAAGCCTATGAAGAAATATGCTAAGATGGCATGGGTAGGAATGGTGTTCCTTGGAGTGATCATGGTTTTGTTGATTATGTTTTGGACTATAAAGGCTAGTCATGAACATTGTTATCATGGTTCAGATGGTTCTGTGGAGCCTCATTGTGAAGTACCAAATTCCTTCAAATCAAGAGCAGTTAAAGAGATTGAGATTTGA
Protein sequence

>MsG0280011125.01.T01

MFIAKGLVSIIVIITLILPQIEAQAVSPGDSAADNVSEQITQRDNTIRVDPLDNFKKYRGGFDITNKNYWSSLIFTGVYGYAIGMLCLLCGILYGVILLITKVYHHKNGGKRMKKVFPCNYKSCDALLIPLAIFLMLIAIVATGLVLVGSARFHSEAKTSVDIIIKTANKASETIHNTTEALKGMESNLMEANVNVEASSNLDSTAEKLDDASVNIEMQAQKNRRLISKGLKIVFITTTVIMCLNLLAVTILSVSGVLRLRRALYLLVVLCWLLTVLCWLFFGVYFFLEKFSSDACTALDNFQENPYNNSLSSILPCQELLKAKPVLSEFSAGIYNLVNEVNANISMQTNLVLVCNPFSAPPNYTYQPENCPDNTIRIGDIPKVLKPFTCLDTNDGSCDNGNFITNSEYARVETYTNSIQDLLNVYPSMEHLLECQIVKDAFSQVLVDHCKPMKKYAKMAWVGMVFLGVIMVLLIMFWTIKASHEHCYHGSDGSVEPHCEVPNSFKSRAVKEIEI*