AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030019.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030019.01.T01 MTR_5g096680 74.790 238 12 1 1 190 1 238 6.78e-123 347
MsG0580030019.01.T01 MTR_8g089695 24.638 207 107 2 33 190 188 394 4.10e-16 75.5
MsG0580030019.01.T01 MTR_8g072140 28.947 114 79 1 44 155 8 121 8.83e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030019.01.T01 AT4G26490 33.503 197 83 1 42 190 72 268 9.12e-38 131
MsG0580030019.01.T01 AT5G56050 33.906 233 95 5 6 190 61 282 2.00e-37 131
MsG0580030019.01.T01 AT1G13050 28.302 159 65 3 81 190 27 185 2.43e-13 65.5
MsG0580030019.01.T01 AT1G13050 28.302 159 65 3 81 190 27 185 2.43e-13 65.5
MsG0580030019.01.T01 AT1G13050 26.842 190 90 2 50 190 128 317 1.99e-12 64.7
MsG0580030019.01.T01 AT3G26350 25.263 190 93 3 50 190 152 341 3.60e-11 61.2
MsG0580030019.01.T01 AT3G26350 25.263 190 93 3 50 190 167 356 4.20e-11 61.2

Find 32 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTACACTTATCTTCTT+TGG 0.214240 5:-105163567 MsG0580030019.01.T01:CDS
CTATGCAAAGGGAACATTTA+AGG 0.223322 5:-105163191 MsG0580030019.01.T01:CDS
AGTCAAAATTTACAACATTT+AGG 0.299761 5:+105163475 None:intergenic
GCATTAGAAATATCAAATAA+AGG 0.310217 5:+105163501 None:intergenic
GGTTGCTTGGTTTGAGTGTA+AGG 0.338313 5:+105163523 None:intergenic
AAGAAAGTTGGCAATACTTA+AGG 0.339972 5:-105163652 MsG0580030019.01.T01:CDS
TTTGATATGATAGGTTGTTC+AGG 0.370467 5:+105163714 None:intergenic
AAAGGGAGTTGCAAAACAAT+AGG 0.383249 5:-105163222 MsG0580030019.01.T01:CDS
CATTAGAAATATCAAATAAA+GGG 0.385546 5:+105163502 None:intergenic
CTTGATGATCTTGTTCTAAG+AGG 0.391536 5:+105163672 None:intergenic
AAAAGACCAAACCAATTGTA+TGG 0.403433 5:-105163617 MsG0580030019.01.T01:CDS
TTCTGAGGGTGGAGATGTGT+TGG 0.412326 5:+105163758 None:intergenic
AGCAAACCATACAATTGGTT+TGG 0.414083 5:+105163611 None:intergenic
GACTCAAAGCAATACTTCAA+TGG 0.427271 5:-105163456 MsG0580030019.01.T01:CDS
ATTGCAGCAAACCATACAAT+TGG 0.442844 5:+105163606 None:intergenic
ATATCAAATAAAGGGTTGCT+TGG 0.463721 5:+105163510 None:intergenic
AACAAGATCATCAAGAAAGT+TGG 0.467969 5:-105163664 MsG0580030019.01.T01:CDS
TTAAGGTGAAGGTTAATATG+TGG 0.477400 5:-105163174 MsG0580030019.01.T01:CDS
TTCAATATTAGTTTGTTCTG+AGG 0.495171 5:+105163743 None:intergenic
TCAATATTAGTTTGTTCTGA+GGG 0.505732 5:+105163744 None:intergenic
TCTGAGGGTGGAGATGTGTT+GGG 0.505757 5:+105163759 None:intergenic
GAAGTTGTGTGAAACTTCAA+AGG 0.506980 5:-105163240 MsG0580030019.01.T01:CDS
GTGGACCAGTCATCTCAATC+TGG 0.507976 5:+105163116 None:intergenic
CTAGCTACAAGAGAACCATT+TGG 0.518577 5:+105163094 None:intergenic
GAGATGACTGGTCCACCAAA+TGG 0.523109 5:-105163109 MsG0580030019.01.T01:CDS
AAAGGGAACATTTAAGGTGA+AGG 0.548043 5:-105163185 MsG0580030019.01.T01:CDS
TAGGTTGAGTTGCTATGCAA+AGG 0.548893 5:-105163203 MsG0580030019.01.T01:CDS
AGGTTGAGTTGCTATGCAAA+GGG 0.552779 5:-105163202 MsG0580030019.01.T01:CDS
ATATTAGTTTGTTCTGAGGG+TGG 0.577316 5:+105163747 None:intergenic
GCTACAAGAGAACCATTTGG+TGG 0.579822 5:+105163097 None:intergenic
AAGTTGTGTGAAACTTCAAA+GGG 0.583012 5:-105163239 MsG0580030019.01.T01:CDS
GTGTGCCAGATTGAGATGAC+TGG 0.624651 5:-105163121 MsG0580030019.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTAGAAATATCAAATAAA+GGG + Chr5:105163338-105163357 None:intergenic 15.0%
!! AGTCAAAATTTACAACATTT+AGG + Chr5:105163365-105163384 None:intergenic 20.0%
!! GCATTAGAAATATCAAATAA+AGG + Chr5:105163339-105163358 None:intergenic 20.0%
! AAAGGAAACTAGATATCAAA+CGG - Chr5:105163539-105163558 MsG0580030019.01.T01:CDS 25.0%
!! GGATTATACATTCATCTTTT+TGG - Chr5:105163684-105163703 MsG0580030019.01.T01:CDS 25.0%
!! TTCAACTTTTCTACTCAAAT+AGG - Chr5:105163475-105163494 MsG0580030019.01.T01:CDS 25.0%
!!! TCAATATTAGTTTGTTCTGA+GGG + Chr5:105163096-105163115 None:intergenic 25.0%
!!! TTCAATATTAGTTTGTTCTG+AGG + Chr5:105163097-105163116 None:intergenic 25.0%
!!! TTGGTTTGTTTTGATATGAT+AGG + Chr5:105163135-105163154 None:intergenic 25.0%
AAAAGACCAAACCAATTGTA+TGG - Chr5:105163220-105163239 MsG0580030019.01.T01:CDS 30.0%
AAACTAGATATCAAACGGTA+AGG - Chr5:105163544-105163563 MsG0580030019.01.T01:CDS 30.0%
AACAAGATCATCAAGAAAGT+TGG - Chr5:105163173-105163192 MsG0580030019.01.T01:CDS 30.0%
AAGAAAGTTGGCAATACTTA+AGG - Chr5:105163185-105163204 MsG0580030019.01.T01:CDS 30.0%
AAGTTGTGTGAAACTTCAAA+GGG - Chr5:105163598-105163617 MsG0580030019.01.T01:CDS 30.0%
AGCTTTACACTTATCTTCTT+TGG - Chr5:105163270-105163289 MsG0580030019.01.T01:intron 30.0%
ATATCAAATAAAGGGTTGCT+TGG + Chr5:105163330-105163349 None:intergenic 30.0%
TTAAGGTGAAGGTTAATATG+TGG - Chr5:105163663-105163682 MsG0580030019.01.T01:CDS 30.0%
TTTGATATGATAGGTTGTTC+AGG + Chr5:105163126-105163145 None:intergenic 30.0%
! CTCACATGAACTTTTCTATT+GGG + Chr5:105163438-105163457 None:intergenic 30.0%
! GTTTGATATCTAGTTTCCTT+TGG + Chr5:105163540-105163559 None:intergenic 30.0%
!!! TCTAAGAGGATGTTGATTTT+TGG + Chr5:105163154-105163173 None:intergenic 30.0%
AAAGGGAGTTGCAAAACAAT+AGG - Chr5:105163615-105163634 MsG0580030019.01.T01:CDS 35.0%
AAGTTTCACACAACTTCTTG+AGG + Chr5:105163594-105163613 None:intergenic 35.0%
AGCAAACCATACAATTGGTT+TGG + Chr5:105163229-105163248 None:intergenic 35.0%
ATTGCAGCAAACCATACAAT+TGG + Chr5:105163234-105163253 None:intergenic 35.0%
CCAATAGAAAAGTTCATGTG+AGG - Chr5:105163436-105163455 MsG0580030019.01.T01:CDS 35.0%
CTATGCAAAGGGAACATTTA+AGG - Chr5:105163646-105163665 MsG0580030019.01.T01:CDS 35.0%
GAAGTTGTGTGAAACTTCAA+AGG - Chr5:105163597-105163616 MsG0580030019.01.T01:CDS 35.0%
GACTCAAAGCAATACTTCAA+TGG - Chr5:105163381-105163400 MsG0580030019.01.T01:intron 35.0%
! AAAGGGAACATTTAAGGTGA+AGG - Chr5:105163652-105163671 MsG0580030019.01.T01:CDS 35.0%
! CCTCACATGAACTTTTCTAT+TGG + Chr5:105163439-105163458 None:intergenic 35.0%
! CTTGATGATCTTGTTCTAAG+AGG + Chr5:105163168-105163187 None:intergenic 35.0%
!! ATATTAGTTTGTTCTGAGGG+TGG + Chr5:105163093-105163112 None:intergenic 35.0%
!!! CAATTGGTTTGGTCTTTTGT+TGG + Chr5:105163218-105163237 None:intergenic 35.0%
CTAGCTACAAGAGAACCATT+TGG + Chr5:105163746-105163765 None:intergenic 40.0%
! AGGTTGAGTTGCTATGCAAA+GGG - Chr5:105163635-105163654 MsG0580030019.01.T01:CDS 40.0%
! TAGGTTGAGTTGCTATGCAA+AGG - Chr5:105163634-105163653 MsG0580030019.01.T01:CDS 40.0%
! TCCTTTGGCTTCTGAGTAAA+AGG + Chr5:105163525-105163544 None:intergenic 40.0%
GCTACAAGAGAACCATTTGG+TGG + Chr5:105163743-105163762 None:intergenic 45.0%
! GCCTTTTACTCAGAAGCCAA+AGG - Chr5:105163521-105163540 MsG0580030019.01.T01:CDS 45.0%
! GGTTGCTTGGTTTGAGTGTA+AGG + Chr5:105163317-105163336 None:intergenic 45.0%
GTGGACCAGTCATCTCAATC+TGG + Chr5:105163724-105163743 None:intergenic 50.0%
GTGTGCCAGATTGAGATGAC+TGG - Chr5:105163716-105163735 MsG0580030019.01.T01:CDS 50.0%
TCTGAGGGTGGAGATGTGTT+GGG + Chr5:105163081-105163100 None:intergenic 50.0%
! GAGATGACTGGTCCACCAAA+TGG - Chr5:105163728-105163747 MsG0580030019.01.T01:CDS 50.0%
! TTCTGAGGGTGGAGATGTGT+TGG + Chr5:105163082-105163101 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 105163072 105163787 105163072 ID=MsG0580030019.01;Name=MsG0580030019.01
Chr5 mRNA 105163072 105163787 105163072 ID=MsG0580030019.01.T01;Parent=MsG0580030019.01;Name=MsG0580030019.01.T01;_AED=0.18;_eAED=0.24;_QI=0|0|0|1|0|0|2|0|190
Chr5 exon 105163072 105163263 105163072 ID=MsG0580030019.01.T01:exon:5941;Parent=MsG0580030019.01.T01
Chr5 exon 105163407 105163787 105163407 ID=MsG0580030019.01.T01:exon:5940;Parent=MsG0580030019.01.T01
Chr5 CDS 105163407 105163787 105163407 ID=MsG0580030019.01.T01:cds;Parent=MsG0580030019.01.T01
Chr5 CDS 105163072 105163263 105163072 ID=MsG0580030019.01.T01:cds;Parent=MsG0580030019.01.T01
Gene Sequence

>MsG0580030019.01.T01

ATGCTTCCCAACACATCTCCACCCTCAGAACAAACTAATATTGAAATTTCTCCTGAACAACCTATCATATCAAAACAAACCAAAAATCAACATCCTCTTAGAACAAGATCATCAAGAAAGTTGGCAATACTTAAGGTTGATGACCAACAAAAGACCAAACCAATTGTATGGTTTGCTGCAATACTATGTTTCATATTCAGCTTTACACTTATCTTCTTTGGAATTGCAACAATAATTTGTTACCTTACACTCAAACCAAGCAACCCTTTATTTGATATTTCTAATGCAAGCCTAAATGTTGTAAATTTTGACTCAAAGCAATACTTCAATGGTGATTTTGCTCTTCAAACAAAATTTTCTAATCCCAATAGAAAAGTTCATAGAAGTTGTGTGAAACTTCAAAGGGAGTTGCAAAACAATAGGTTGAGTTGCTATGCAAAGGGAACATTTAAGGTGAAGGTTAATATGTGGATTATACATTCATCTTTTTGGCTTCATAGTGTGTGCCAGATTGAGATGACTGGTCCACCAAATGGTTCTCTTGTAGCTAGACAATGTATAACAACTCGATGA

Protein sequence

>MsG0580030019.01.T01

MLPNTSPPSEQTNIEISPEQPIISKQTKNQHPLRTRSSRKLAILKVDDQQKTKPIVWFAAILCFIFSFTLIFFGIATIICYLTLKPSNPLFDISNASLNVVNFDSKQYFNGDFALQTKFSNPNRKVHRSCVKLQRELQNNRLSCYAKGTFKVKVNMWIIHSSFWLHSVCQIEMTGPPNGSLVARQCITTR*