Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013973.01.T01 | YP_009141634.1 | 100 | 55 | 0 | 0 | 1 | 55 | 141 | 195 | 4.51E-31 | 116 |
| MsG0380013973.01.T02 | YP_009141634.1 | 100 | 46 | 0 | 0 | 1 | 46 | 150 | 195 | 6.31E-24 | 97.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013973.01.T01 | A6MMW9 | 68.519 | 54 | 17 | 0 | 1 | 54 | 141 | 194 | 2.84E-21 | 84.3 |
| MsG0380013973.01.T02 | A6MMW9 | 66.667 | 45 | 15 | 0 | 1 | 45 | 150 | 194 | 1.53E-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013973.01.T01 | A0A109RTM9 | 100.000 | 55 | 0 | 0 | 1 | 55 | 141 | 195 | 2.15e-31 | 116 |
| MsG0380013973.01.T02 | A0A109RTM9 | 100.000 | 46 | 0 | 0 | 1 | 46 | 150 | 195 | 3.02e-24 | 97.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013933.01 | MsG0380013973.01 | 0.843391 | 1.441897e-58 | 2.252926e-55 |
| MsG0380013973.01 | MsG0380013974.01 | 0.962636 | 4.100509e-121 | 2.074136e-115 |
| MsG0380013973.01 | MsG0380014047.01 | 0.975875 | 9.213798e-141 | 1.470374e-134 |
| MsG0380013973.01 | MsG0380014049.01 | 0.827471 | 1.536549e-54 | 1.484851e-51 |
| MsG0380013973.01 | MsG0680033965.01 | 0.827764 | 1.306203e-54 | 1.273002e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013973.01.T01 | MTR_4g077730 | 92.593 | 54 | 4 | 0 | 1 | 54 | 18 | 71 | 1.13e-32 | 107 |
| MsG0380013973.01.T01 | MTR_4g034690 | 92.593 | 54 | 4 | 0 | 1 | 54 | 141 | 194 | 1.38e-31 | 107 |
| MsG0380013973.01.T01 | MTR_0002s0730 | 56.364 | 55 | 24 | 0 | 1 | 55 | 141 | 195 | 6.20e-17 | 70.9 |
| MsG0380013973.01.T02 | MTR_4g077730 | 93.333 | 45 | 3 | 0 | 1 | 45 | 27 | 71 | 4.23e-27 | 92.4 |
| MsG0380013973.01.T02 | MTR_4g034690 | 93.333 | 45 | 3 | 0 | 1 | 45 | 150 | 194 | 1.58e-25 | 92.0 |
| MsG0380013973.01.T02 | MTR_0002s0730 | 56.522 | 46 | 20 | 0 | 1 | 46 | 150 | 195 | 1.15e-13 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013973.01.T01 | ATCG00670 | 70.370 | 54 | 16 | 0 | 1 | 54 | 141 | 194 | 3.30e-21 | 81.6 |
| MsG0380013973.01.T02 | ATCG00670 | 68.889 | 45 | 14 | 0 | 1 | 45 | 150 | 194 | 4.94e-17 | 70.1 |
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAACAGGCAAAGCTTCC+TGG | 0.339983 | 3:-48546745 | MsG0380013973.01.T01:CDS |
| AATATTTATGCACAAAGAAC+AGG | 0.440622 | 3:-48546758 | MsG0380013973.01.T01:CDS |
| CATGTCTTTGTATATCTGCC+AGG | 0.488452 | 3:+48546727 | None:intergenic |
| AGATATACAAAGACATGGAA+AGG | 0.533735 | 3:-48546721 | MsG0380013973.01.T01:CDS |
| GATATACAAAGACATGGAAA+GGG | 0.558351 | 3:-48546720 | MsG0380013973.01.T01:CDS |
| CTGGCAGATATACAAAGACA+TGG | 0.577986 | 3:-48546726 | MsG0380013973.01.T01:CDS |
| GCAGAAGAAGCTCAAGCCCA+CGG | 0.589230 | 3:-48546683 | MsG0380013973.01.T01:CDS |
| GACCGTATCAACAATTCCGT+GGG | 0.592391 | 3:+48546667 | None:intergenic |
| AGCCCACGGAATTGTTGATA+CGG | 0.594347 | 3:-48546669 | MsG0380013973.01.T01:CDS |
| CGACCGTATCAACAATTCCG+TGG | 0.614764 | 3:+48546666 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATTTATGCACAAAGAAC+AGG | - | Chr3:48546703-48546722 | MsG0380013973.01.T01:CDS | 25.0% |
| AGATATACAAAGACATGGAA+AGG | - | Chr3:48546740-48546759 | MsG0380013973.01.T01:CDS | 30.0% | |
| GATATACAAAGACATGGAAA+GGG | - | Chr3:48546741-48546760 | MsG0380013973.01.T01:CDS | 30.0% | |
| CATGTCTTTGTATATCTGCC+AGG | + | Chr3:48546737-48546756 | None:intergenic | 40.0% | |
| CTGGCAGATATACAAAGACA+TGG | - | Chr3:48546735-48546754 | MsG0380013973.01.T01:CDS | 40.0% | |
| AAAGAACAGGCAAAGCTTCC+TGG | - | Chr3:48546716-48546735 | MsG0380013973.01.T01:CDS | 45.0% | |
| AGCCCACGGAATTGTTGATA+CGG | - | Chr3:48546792-48546811 | MsG0380013973.01.T01:CDS | 45.0% | |
| GACCGTATCAACAATTCCGT+GGG | + | Chr3:48546797-48546816 | None:intergenic | 45.0% | |
| CGACCGTATCAACAATTCCG+TGG | + | Chr3:48546798-48546817 | None:intergenic | 50.0% | |
| GCAGAAGAAGCTCAAGCCCA+CGG | - | Chr3:48546778-48546797 | MsG0380013973.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48546658 | 48546825 | 48546658 | ID=MsG0380013973.01;Name=MsG0380013973.01 |
| Chr3 | mRNA | 48546658 | 48546825 | 48546658 | ID=MsG0380013973.01.T01;Parent=MsG0380013973.01;Name=MsG0380013973.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|55 |
| Chr3 | exon | 48546658 | 48546825 | 48546658 | ID=MsG0380013973.01.T01:exon:59773;Parent=MsG0380013973.01.T01 |
| Chr3 | CDS | 48546658 | 48546825 | 48546658 | ID=MsG0380013973.01.T01:cds;Parent=MsG0380013973.01.T01 |
| Chr3 | mRNA | 48546658 | 48546798 | 48546658 | ID=MsG0380013973.01.T02;Parent=MsG0380013973.01;Name=MsG0380013973.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|46 |
| Chr3 | exon | 48546658 | 48546798 | 48546658 | ID=MsG0380013973.01.T02:exon:59774;Parent=MsG0380013973.01.T02 |
| Chr3 | CDS | 48546658 | 48546798 | 48546658 | ID=MsG0380013973.01.T02:cds;Parent=MsG0380013973.01.T02 |
| Gene Sequence |
| Protein sequence |
