Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013974.01.T01 | AWW91854.1 | 100 | 124 | 0 | 0 | 1 | 124 | 1 | 124 | 4.02E-85 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013974.01.T01 | P12210 | 78.512 | 121 | 26 | 0 | 1 | 121 | 1 | 121 | 6.55E-68 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013974.01.T01 | A0A2Z4KH16 | 100.000 | 124 | 0 | 0 | 1 | 124 | 1 | 124 | 1.92e-85 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013933.01 | MsG0380013974.01 | 0.876034 | 1.924345e-68 | 9.188245e-65 |
| MsG0380013973.01 | MsG0380013974.01 | 0.962636 | 4.100509e-121 | 2.074136e-115 |
| MsG0380013974.01 | MsG0380014047.01 | 0.917124 | 7.777083e-86 | 2.208653e-81 |
| MsG0380013974.01 | MsG0380014048.01 | 0.801954 | 7.056287e-49 | 3.414036e-46 |
| MsG0380013974.01 | MsG0380014049.01 | 0.844778 | 6.121389e-59 | 9.989507e-56 |
| MsG0380013974.01 | MsG0580027678.01 | 0.808424 | 3.118439e-50 | 1.782425e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013974.01.T01 | MTR_4g034690 | 94.167 | 120 | 7 | 0 | 1 | 120 | 1 | 120 | 1.68e-81 | 237 |
| MsG0380013974.01.T01 | MTR_0002s0730 | 64.463 | 121 | 43 | 0 | 1 | 121 | 1 | 121 | 1.54e-57 | 177 |
| MsG0380013974.01.T01 | MTR_4g102000 | 43.802 | 121 | 68 | 0 | 1 | 121 | 109 | 229 | 8.07e-36 | 125 |
| MsG0380013974.01.T01 | MTR_7g082980 | 43.564 | 101 | 57 | 0 | 21 | 121 | 78 | 178 | 5.83e-27 | 101 |
| MsG0380013974.01.T01 | MTR_1g086940 | 46.392 | 97 | 52 | 0 | 25 | 121 | 114 | 210 | 2.95e-26 | 99.8 |
| MsG0380013974.01.T01 | MTR_4g069800 | 42.574 | 101 | 58 | 0 | 21 | 121 | 98 | 198 | 2.13e-25 | 96.3 |
| MsG0380013974.01.T01 | MTR_7g109610 | 45.361 | 97 | 53 | 0 | 25 | 121 | 111 | 207 | 2.53e-25 | 97.4 |
| MsG0380013974.01.T01 | MTR_4g069800 | 42.574 | 101 | 58 | 0 | 21 | 121 | 98 | 198 | 7.54e-25 | 95.5 |
| MsG0380013974.01.T01 | MTR_4g075100 | 38.000 | 100 | 62 | 0 | 22 | 121 | 45 | 144 | 1.12e-23 | 91.7 |
| MsG0380013974.01.T01 | MTR_5g026840 | 42.000 | 100 | 58 | 0 | 22 | 121 | 88 | 187 | 1.58e-23 | 92.4 |
| MsG0380013974.01.T01 | MTR_4g069800 | 40.179 | 112 | 63 | 2 | 10 | 121 | 59 | 166 | 3.08e-23 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013974.01.T01 | ATCG00670 | 71.074 | 121 | 35 | 0 | 1 | 121 | 1 | 121 | 6.85e-63 | 190 |
| MsG0380013974.01.T01 | AT1G12410 | 42.017 | 119 | 69 | 0 | 1 | 119 | 68 | 186 | 2.41e-34 | 120 |
| MsG0380013974.01.T01 | AT1G66670 | 41.026 | 117 | 66 | 1 | 5 | 121 | 71 | 184 | 3.57e-27 | 102 |
| MsG0380013974.01.T01 | AT1G02560 | 44.444 | 99 | 55 | 0 | 23 | 121 | 115 | 213 | 1.08e-25 | 98.2 |
| MsG0380013974.01.T01 | AT5G23140 | 39.000 | 100 | 61 | 0 | 22 | 121 | 48 | 147 | 3.30e-25 | 95.9 |
| MsG0380013974.01.T01 | AT5G45390 | 40.000 | 100 | 60 | 0 | 22 | 121 | 79 | 178 | 1.70e-22 | 89.7 |
| MsG0380013974.01.T01 | AT1G11750 | 38.614 | 101 | 62 | 0 | 21 | 121 | 95 | 195 | 6.30e-22 | 87.8 |
| MsG0380013974.01.T01 | AT1G11750 | 38.614 | 101 | 62 | 0 | 21 | 121 | 113 | 213 | 8.86e-22 | 87.8 |
| MsG0380013974.01.T01 | AT1G09130 | 34.862 | 109 | 62 | 2 | 22 | 121 | 121 | 229 | 4.07e-14 | 67.4 |
| MsG0380013974.01.T01 | AT1G09130 | 34.862 | 109 | 62 | 2 | 22 | 121 | 121 | 229 | 4.07e-14 | 67.4 |
| MsG0380013974.01.T01 | AT1G09130 | 34.862 | 109 | 62 | 2 | 22 | 121 | 121 | 229 | 5.11e-14 | 67.4 |
| MsG0380013974.01.T01 | AT4G17040 | 31.148 | 122 | 76 | 1 | 8 | 121 | 91 | 212 | 6.36e-14 | 66.6 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAATGGGATCTCTTCTTT+TGG | 0.277134 | 3:-48547548 | MsG0380013974.01.T01:CDS |
| GGAAGTCTAAAAGGTACTTT+TGG | 0.294186 | 3:+48547832 | None:intergenic |
| AAATCAACTTGTTGGTCTAA+TGG | 0.337747 | 3:-48547716 | MsG0380013974.01.T01:CDS |
| AAGTTGCATAATATCAAATA+TGG | 0.346523 | 3:+48547612 | None:intergenic |
| TCATCTTCAGGAAGTCTAAA+AGG | 0.356821 | 3:+48547823 | None:intergenic |
| GAAATATCAAATCAACTTGT+TGG | 0.360879 | 3:-48547724 | MsG0380013974.01.T01:CDS |
| CTGAAGATGATGACGCATCT+TGG | 0.390914 | 3:-48547810 | MsG0380013974.01.T01:CDS |
| CAAGATGCGTCATCATCTTC+AGG | 0.392047 | 3:+48547811 | None:intergenic |
| CCGGGCGGAGAGGTAATATC+TGG | 0.397846 | 3:-48547640 | MsG0380013974.01.T01:CDS |
| GTATTTGTTTATAAATTCTC+CGG | 0.406186 | 3:-48547659 | MsG0380013974.01.T01:CDS |
| AATGGTATATCTCAGTTTAG+AGG | 0.415149 | 3:-48547698 | MsG0380013974.01.T01:CDS |
| CGTAGGATTAGCCGCTTCAA+TGG | 0.438090 | 3:-48547563 | MsG0380013974.01.T01:CDS |
| TGAAGATGATGACGCATCTT+GGG | 0.468266 | 3:-48547809 | MsG0380013974.01.T01:CDS |
| GTGAGGGAATGCTAGACGTT+TGG | 0.491116 | 3:+48547510 | None:intergenic |
| GTCTAGCATTCCCTCACGCT+TGG | 0.526238 | 3:-48547504 | MsG0380013974.01.T01:CDS |
| AATCATTCGCGCCAAGCGTG+AGG | 0.531644 | 3:+48547493 | None:intergenic |
| GTAGGATTAGCCGCTTCAAT+GGG | 0.537674 | 3:-48547562 | MsG0380013974.01.T01:CDS |
| TATTTGTTTATAAATTCTCC+GGG | 0.548583 | 3:-48547658 | MsG0380013974.01.T01:CDS |
| CGGAGAGGTAATATCTGGAA+TGG | 0.554606 | 3:-48547635 | MsG0380013974.01.T01:CDS |
| ATCATTCGCGCCAAGCGTGA+GGG | 0.579806 | 3:+48547494 | None:intergenic |
| CCAGATATTACCTCTCCGCC+CGG | 0.580300 | 3:+48547640 | None:intergenic |
| GAAGTACATACAGTATGCGT+AGG | 0.598626 | 3:-48547580 | MsG0380013974.01.T01:CDS |
| TATAAATTCTCCGGGCGGAG+AGG | 0.603462 | 3:-48547650 | MsG0380013974.01.T01:CDS |
| AAGAAGAGATCCCATTGAAG+CGG | 0.611882 | 3:+48547552 | None:intergenic |
| TTGTTTATAAATTCTCCGGG+CGG | 0.724196 | 3:-48547655 | MsG0380013974.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTGCATAATATCAAATA+TGG | + | Chr3:48547733-48547752 | None:intergenic | 20.0% |
| !! | GTATTTGTTTATAAATTCTC+CGG | - | Chr3:48547683-48547702 | MsG0380013974.01.T01:CDS | 20.0% |
| !! | TATTTGTTTATAAATTCTCC+GGG | - | Chr3:48547684-48547703 | MsG0380013974.01.T01:CDS | 20.0% |
| !!! | CAAGAAAGATTACTTTTTTT+AGG | - | Chr3:48547579-48547598 | MsG0380013974.01.T01:CDS | 20.0% |
| ! | GAAATATCAAATCAACTTGT+TGG | - | Chr3:48547618-48547637 | MsG0380013974.01.T01:CDS | 25.0% |
| !!! | ATTACTTTTTTTAGGTCAAG+AGG | - | Chr3:48547587-48547606 | MsG0380013974.01.T01:CDS | 25.0% |
| AATGGTATATCTCAGTTTAG+AGG | - | Chr3:48547644-48547663 | MsG0380013974.01.T01:CDS | 30.0% | |
| !! | AAATCAACTTGTTGGTCTAA+TGG | - | Chr3:48547626-48547645 | MsG0380013974.01.T01:CDS | 30.0% |
| TCATCTTCAGGAAGTCTAAA+AGG | + | Chr3:48547522-48547541 | None:intergenic | 35.0% | |
| TTGTTTATAAATTCTCCGGG+CGG | - | Chr3:48547687-48547706 | MsG0380013974.01.T01:CDS | 35.0% | |
| ! | TTCAATGGGATCTCTTCTTT+TGG | - | Chr3:48547794-48547813 | MsG0380013974.01.T01:CDS | 35.0% |
| !!! | GGAAGTCTAAAAGGTACTTT+TGG | + | Chr3:48547513-48547532 | None:intergenic | 35.0% |
| AAGAAGAGATCCCATTGAAG+CGG | + | Chr3:48547793-48547812 | None:intergenic | 40.0% | |
| GAAGTACATACAGTATGCGT+AGG | - | Chr3:48547762-48547781 | MsG0380013974.01.T01:CDS | 40.0% | |
| ! | ATGGGATCTCTTCTTTTGGT+CGG | - | Chr3:48547798-48547817 | MsG0380013974.01.T01:CDS | 40.0% |
| ! | TGAAGATGATGACGCATCTT+GGG | - | Chr3:48547533-48547552 | MsG0380013974.01.T01:CDS | 40.0% |
| !!! | GGTACTTTTGGAACACCAAT+TGG | + | Chr3:48547501-48547520 | None:intergenic | 40.0% |
| CAAGATGCGTCATCATCTTC+AGG | + | Chr3:48547534-48547553 | None:intergenic | 45.0% | |
| CGGAGAGGTAATATCTGGAA+TGG | - | Chr3:48547707-48547726 | MsG0380013974.01.T01:CDS | 45.0% | |
| ! | CTGAAGATGATGACGCATCT+TGG | - | Chr3:48547532-48547551 | MsG0380013974.01.T01:CDS | 45.0% |
| !! | GTAGGATTAGCCGCTTCAAT+GGG | - | Chr3:48547780-48547799 | MsG0380013974.01.T01:CDS | 45.0% |
| GTGAGGGAATGCTAGACGTT+TGG | + | Chr3:48547835-48547854 | None:intergenic | 50.0% | |
| TATAAATTCTCCGGGCGGAG+AGG | - | Chr3:48547692-48547711 | MsG0380013974.01.T01:CDS | 50.0% | |
| ! | GGATCTCTTCTTTTGGTCGG+AGG | - | Chr3:48547801-48547820 | MsG0380013974.01.T01:CDS | 50.0% |
| !! | CGTAGGATTAGCCGCTTCAA+TGG | - | Chr3:48547779-48547798 | MsG0380013974.01.T01:CDS | 50.0% |
| CCAGATATTACCTCTCCGCC+CGG | + | Chr3:48547705-48547724 | None:intergenic | 55.0% | |
| GTCTAGCATTCCCTCACGCT+TGG | - | Chr3:48547838-48547857 | MsG0380013974.01.T01:CDS | 55.0% | |
| CCGGGCGGAGAGGTAATATC+TGG | - | Chr3:48547702-48547721 | MsG0380013974.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48547495 | 48547869 | 48547495 | ID=MsG0380013974.01;Name=MsG0380013974.01 |
| Chr3 | mRNA | 48547495 | 48547869 | 48547495 | ID=MsG0380013974.01.T01;Parent=MsG0380013974.01;Name=MsG0380013974.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|124 |
| Chr3 | exon | 48547495 | 48547869 | 48547495 | ID=MsG0380013974.01.T01:exon:11256;Parent=MsG0380013974.01.T01 |
| Chr3 | CDS | 48547495 | 48547869 | 48547495 | ID=MsG0380013974.01.T01:cds;Parent=MsG0380013974.01.T01 |
| Gene Sequence |
| Protein sequence |
