AlfalfaGEDB | MsG0380013933.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013933.01

KEGG: K02913
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00471

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013933.01.T01	YP_009141631.1	100	66	0	0	1	66	1	66	7.79E-40	135

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013933.01.T01	B5LMP5	96.97	66	2	0	1	66	1	66	6.84E-42	133

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013933.01.T01	A0A4V1E3D4	100.000	66	0	0	1	66	1	66	3.72e-40	135

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048845.01	MsG0380013933.01	0.801278	9.710882e-49	4.618942e-46
MsG0080049078.01	MsG0380013933.01	0.806591	7.636472e-50	4.161000e-47
MsG0180005568.01	MsG0380013933.01	0.850566	1.563575e-60	3.075449e-57
MsG0180005829.01	MsG0380013933.01	0.809431	1.899175e-50	1.114865e-47
MsG0280006349.01	MsG0380013933.01	0.826807	2.214651e-54	2.098944e-51
MsG0280011270.01	MsG0380013933.01	0.813127	2.994857e-51	1.939169e-48
MsG0380013933.01	MsG0380013939.01	0.803851	2.862158e-49	1.453686e-46
MsG0380013933.01	MsG0380013955.01	0.844031	9.722258e-59	1.549832e-55
MsG0380013933.01	MsG0380013965.01	0.817186	3.758200e-52	2.717996e-49
MsG0380013933.01	MsG0380013970.01	0.829005	6.557997e-55	6.626319e-52
MsG0380013933.01	MsG0380013973.01	0.843391	1.441897e-58	2.252926e-55
MsG0380013933.01	MsG0380013974.01	0.876034	1.924345e-68	9.188245e-65
MsG0380013933.01	MsG0380013996.01	0.804195	2.427611e-49	1.243847e-46
MsG0380013933.01	MsG0380014007.01	0.840718	7.343822e-58	1.055214e-54
MsG0380013933.01	MsG0380014012.01	0.830937	2.216214e-55	2.369599e-52
MsG0380013933.01	MsG0380014013.01	0.820570	6.395654e-53	5.078944e-50
MsG0380013933.01	MsG0380014022.01	0.823028	1.726963e-53	1.469931e-50
MsG0380013933.01	MsG0380014038.01	0.805264	1.452101e-49	7.647646e-47
MsG0380013933.01	MsG0380014048.01	0.807341	5.299885e-50	2.944560e-47
MsG0380013933.01	MsG0380014049.01	0.914007	3.173749e-84	7.720611e-80
MsG0380013933.01	MsG0380015545.01	0.803802	2.929869e-49	1.486157e-46
MsG0380013933.01	MsG0380015921.01	0.804392	2.208786e-49	1.137483e-46
MsG0380013933.01	MsG0380016762.01	0.818076	2.367735e-52	1.754637e-49
MsG0380013933.01	MsG0380017033.01	0.817685	2.900997e-52	2.127013e-49
MsG0380013933.01	MsG0480018089.01	0.809007	2.341042e-50	1.358693e-47
MsG0380013933.01	MsG0480021168.01	0.817835	2.682890e-52	1.975278e-49
MsG0380013933.01	MsG0480021420.01	0.802778	4.774584e-49	2.359257e-46
MsG0380013933.01	MsG0480022918.01	0.820139	8.031830e-53	6.303314e-50
MsG0380013933.01	MsG0480023578.01	0.801318	9.527934e-49	4.536456e-46
MsG0380013933.01	MsG0580024717.01	0.810022	1.417042e-50	8.449581e-48
MsG0380013933.01	MsG0580025589.01	0.809759	1.614192e-50	9.559255e-48
MsG0380013933.01	MsG0580025625.01	0.804104	2.535512e-49	1.296145e-46
MsG0380013933.01	MsG0580028925.01	0.814379	1.587455e-51	1.063505e-48
MsG0380013933.01	MsG0580029414.01	0.823880	1.091578e-53	9.517442e-51
MsG0380013933.01	MsG0680033224.01	0.803183	3.937372e-49	1.965685e-46
MsG0380013933.01	MsG0680033966.01	0.830600	2.680195e-55	2.837223e-52
MsG0380013933.01	MsG0780037768.01	0.808686	2.741719e-50	1.577737e-47
MsG0380013933.01	MsG0780037946.01	0.826526	2.585568e-54	2.430743e-51
MsG0380013933.01	MsG0780039233.01	0.816761	4.681067e-52	3.346406e-49
MsG0380013933.01	MsG0780039975.01	0.828684	7.839272e-55	7.847867e-52
MsG0380013933.01	MsG0780041004.01	0.845204	4.699159e-59	7.772179e-56
MsG0380013933.01	MsG0780041262.01	0.801969	7.005086e-49	3.390545e-46
MsG0380013933.01	MsG0880044444.01	0.851935	6.418000e-61	1.319548e-57
MsG0380013933.01	MsG0880044538.01	0.818244	2.169136e-52	1.614891e-49
MsG0380013933.01	MsG0880044558.01	0.812492	4.125060e-51	2.626630e-48
MsG0380013933.01	MsG0880045283.01	0.805048	1.610827e-49	8.437214e-47
MsG0380013933.01	MsG0880046288.01	0.803704	3.069619e-49	1.553087e-46
MsG0380013933.01	MsG0880046735.01	0.819729	9.969336e-53	7.734283e-50
MsG0380013933.01	MsG0880047460.01	-0.814982	1.167630e-51	7.950377e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013933.01.T01	MTR_8g045210	96.774	62	2	0	4	65	36	97	3.80e-40	127
MsG0380013933.01.T01	MTR_4g051350	98.361	61	1	0	1	61	1	61	4.94e-40	130

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013933.01.T01	ATCG00640	77.273	66	15	0	1	66	1	66	1.33e-34	112

Find 11 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATACGAATAACTGTTATTT+TGG	0.125276	3:-48455419	MsG0380013933.01.T01:CDS
AGAGTGTTAATAAGGAATCT+AGG	0.329597	3:-48455375	MsG0380013933.01.T01:CDS
AATCGTATGTTTGCAACAAA+AGG	0.396841	3:+48455279	None:intergenic
GAGTGTTAATAAGGAATCTA+GGG	0.452009	3:-48455374	MsG0380013933.01.T01:CDS
TATTCGTATATCTTTACCCT+TGG	0.471315	3:+48455432	None:intergenic
CCAAAGGAGGTTCATGGCCA+AGG	0.512674	3:-48455449	None:intergenic
ACACAATACACCTAGTCGAT+TGG	0.524208	3:-48455320	MsG0380013933.01.T01:CDS
ATCGTATGTTTGCAACAAAA+GGG	0.538893	3:+48455280	None:intergenic
TTTCTCAATTCCAATCGACT+AGG	0.574270	3:+48455310	None:intergenic
CAAAGGAGGTTCATGGCCAA+GGG	0.605158	3:-48455448	None:intergenic
AGTGTTAATAAGGAATCTAG+GGG	0.667965	3:-48455373	MsG0380013933.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATACGAATAACTGTTATTT+TGG	-	Chr3:48455275-48455294	MsG0380013933.01.T01:CDS	20.0%
!!!	TGATAAAAAGAGTGTTAATA+AGG	-	Chr3:48455311-48455330	MsG0380013933.01.T01:CDS	20.0%
	AATCGTATGTTTGCAACAAA+AGG	+	Chr3:48455418-48455437	None:intergenic	30.0%
	AGTGTTAATAAGGAATCTAG+GGG	-	Chr3:48455321-48455340	MsG0380013933.01.T01:CDS	30.0%
	ATCGTATGTTTGCAACAAAA+GGG	+	Chr3:48455417-48455436	None:intergenic	30.0%
	GAGTGTTAATAAGGAATCTA+GGG	-	Chr3:48455320-48455339	MsG0380013933.01.T01:CDS	30.0%
	TATTCGTATATCTTTACCCT+TGG	+	Chr3:48455265-48455284	None:intergenic	30.0%
!	AGAGTGTTAATAAGGAATCT+AGG	-	Chr3:48455319-48455338	MsG0380013933.01.T01:CDS	30.0%
	TTTCTCAATTCCAATCGACT+AGG	+	Chr3:48455387-48455406	None:intergenic	35.0%
	ACACAATACACCTAGTCGAT+TGG	-	Chr3:48455374-48455393	MsG0380013933.01.T01:CDS	40.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48455258	48455458	48455258	ID=MsG0380013933.01;Name=MsG0380013933.01
Chr3	mRNA	48455258	48455458	48455258	ID=MsG0380013933.01.T01;Parent=MsG0380013933.01;Name=MsG0380013933.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|66
Chr3	exon	48455258	48455458	48455258	ID=MsG0380013933.01.T01:exon:11483;Parent=MsG0380013933.01.T01
Chr3	CDS	48455258	48455458	48455258	ID=MsG0380013933.01.T01:cds;Parent=MsG0380013933.01.T01

Gene Sequence	>MsG0380013933.01.T01 ATGGCCAAGGGTAAAGATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG
Protein sequence	>MsG0380013933.01.T01 MAKGKDIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*