AlfalfaGEDB | MsG0380014049.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014049.01

KEGG: K02963
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF01084

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014049.01.T01	YP_009141632.1	95.745	94	4	0	1	94	1	94	4.53E-55	176
MsG0380014049.01.T02	YP_009141632.1	93.548	62	4	0	1	62	33	94	6.57E-31	114

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014049.01.T01	P49169	81.72	93	17	0	1	93	1	93	2.49E-45	144
MsG0380014049.01.T02	P49169	80.328	61	12	0	1	61	33	93	4.63E-26	94.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014049.01.T01	A0A0F6NLD5	95.745	94	4	0	1	94	1	94	2.16e-55	176
MsG0380014049.01.T02	A0A0X8GKL0	93.548	62	4	0	1	62	33	94	3.14e-31	114

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048845.01	MsG0380014049.01	0.809240	2.086437e-50	1.218615e-47
MsG0080049078.01	MsG0380014049.01	0.825163	5.443492e-54	4.923343e-51
MsG0180000843.01	MsG0380014049.01	0.815171	1.060092e-51	7.254844e-49
MsG0180004188.01	MsG0380014049.01	0.814442	1.537327e-51	1.031668e-48
MsG0180005119.01	MsG0380014049.01	0.822894	1.855498e-53	1.573307e-50
MsG0180005391.01	MsG0380014049.01	0.813852	2.075501e-51	1.370646e-48
MsG0180005568.01	MsG0380014049.01	0.874711	5.453674e-68	2.479889e-64
MsG0180005829.01	MsG0380014049.01	0.850556	1.573503e-60	3.094073e-57
MsG0280006349.01	MsG0380014049.01	0.825547	4.416477e-54	4.037956e-51
MsG0280010975.01	MsG0380014049.01	0.802014	6.859952e-49	3.324191e-46
MsG0280011270.01	MsG0380014049.01	0.813196	2.893467e-51	1.877045e-48
MsG0380013927.01	MsG0380014049.01	0.809609	1.738753e-50	1.025595e-47
MsG0380013933.01	MsG0380014049.01	0.914007	3.173749e-84	7.720611e-80
MsG0380013955.01	MsG0380014049.01	0.860144	2.534149e-63	6.858081e-60
MsG0380013965.01	MsG0380014049.01	0.823218	1.559367e-53	1.334311e-50
MsG0380013970.01	MsG0380014049.01	0.846995	1.529326e-59	2.679782e-56
MsG0380013973.01	MsG0380014049.01	0.827471	1.536549e-54	1.484851e-51
MsG0380013974.01	MsG0380014049.01	0.844778	6.121389e-59	9.989507e-56
MsG0380014007.01	MsG0380014049.01	0.861187	1.223485e-63	3.432028e-60
MsG0380014012.01	MsG0380014049.01	0.827250	1.735419e-54	1.666307e-51
MsG0380014013.01	MsG0380014049.01	0.832979	6.938289e-56	7.881695e-53
MsG0380014022.01	MsG0380014049.01	0.827128	1.856508e-54	1.776037e-51
MsG0380014037.01	MsG0380014049.01	0.801480	8.827990e-49	4.220427e-46
MsG0380014049.01	MsG0380015754.01	0.804334	2.270256e-49	1.167433e-46
MsG0380014049.01	MsG0380016284.01	0.801516	8.679927e-49	4.153381e-46
MsG0380014049.01	MsG0380016452.01	0.822661	2.101698e-53	1.770389e-50
MsG0380014049.01	MsG0380016459.01	0.805282	1.439244e-49	7.583648e-47
MsG0380014049.01	MsG0380016762.01	0.823086	1.674118e-53	1.427296e-50
MsG0380014049.01	MsG0380017033.01	0.833698	4.593074e-56	5.330396e-53
MsG0380014049.01	MsG0380017661.01	0.808790	2.605338e-50	1.503356e-47
MsG0380014049.01	MsG0380018037.01	0.805434	1.337220e-49	7.073944e-47
MsG0380014049.01	MsG0480018089.01	0.807940	3.953865e-50	2.231193e-47
MsG0380014049.01	MsG0480018323.01	0.820079	8.290997e-53	6.495879e-50
MsG0380014049.01	MsG0480020229.01	0.819238	1.289779e-52	9.870113e-50
MsG0380014049.01	MsG0480021168.01	0.822172	2.730040e-53	2.267659e-50
MsG0380014049.01	MsG0480021294.01	0.825718	4.024368e-54	3.697484e-51
MsG0380014049.01	MsG0480021367.01	0.812173	4.844030e-51	3.058000e-48
MsG0380014049.01	MsG0480021377.01	0.804945	1.692687e-49	8.843066e-47
MsG0380014049.01	MsG0480021420.01	0.809021	2.325150e-50	1.349966e-47
MsG0380014049.01	MsG0480021999.01	0.801807	7.563294e-49	3.645842e-46
MsG0380014049.01	MsG0480022617.01	0.802853	4.605341e-49	2.279969e-46
MsG0380014049.01	MsG0480022918.01	0.837084	6.402075e-57	8.225728e-54
MsG0380014049.01	MsG0480023166.01	0.819598	1.067563e-52	8.251994e-50
MsG0380014049.01	MsG0480023216.01	0.802667	5.031670e-49	2.479136e-46
MsG0380014049.01	MsG0480023578.01	0.804659	1.942792e-49	1.007432e-46
MsG0380014049.01	MsG0480023992.01	0.802179	6.343490e-49	3.086816e-46
MsG0380014049.01	MsG0580024717.01	0.829477	5.036502e-55	5.160519e-52
MsG0380014049.01	MsG0580024759.01	0.815832	7.552772e-52	5.263549e-49
MsG0380014049.01	MsG0580025589.01	0.810640	1.042597e-50	6.318576e-48
MsG0380014049.01	MsG0580025625.01	0.826759	2.274038e-54	2.152201e-51
MsG0380014049.01	MsG0580027098.01	0.809120	2.213320e-50	1.288581e-47
MsG0380014049.01	MsG0580028024.01	0.828158	1.050066e-54	1.035293e-51
MsG0380014049.01	MsG0580028925.01	0.822580	2.195347e-53	1.845130e-50
MsG0380014049.01	MsG0580029276.01	0.801188	1.012948e-48	4.807117e-46
MsG0380014049.01	MsG0580029414.01	0.833015	6.797333e-56	7.729968e-53
MsG0380014049.01	MsG0680030464.01	0.805540	1.270488e-49	6.739044e-47
MsG0380014049.01	MsG0680030680.01	0.815912	7.247476e-52	5.061908e-49
MsG0380014049.01	MsG0680032006.01	0.801258	9.800499e-49	4.659208e-46
MsG0380014049.01	MsG0680033224.01	0.803481	3.415089e-49	1.718105e-46
MsG0380014049.01	MsG0680033966.01	0.805815	1.112479e-49	5.943040e-47
MsG0380014049.01	MsG0780036567.01	0.838283	3.152933e-57	4.202556e-54
MsG0380014049.01	MsG0780037662.01	0.807159	5.791590e-50	3.202469e-47
MsG0380014049.01	MsG0780037705.01	0.804084	2.559697e-49	1.307859e-46
MsG0380014049.01	MsG0780037946.01	0.816605	5.074028e-52	3.611868e-49
MsG0380014049.01	MsG0780039233.01	0.821632	3.641130e-53	2.978373e-50
MsG0380014049.01	MsG0780039256.01	0.804060	2.589937e-49	1.322518e-46
MsG0380014049.01	MsG0780039975.01	0.840769	7.123584e-58	1.025178e-54
MsG0380014049.01	MsG0780040314.01	0.802887	4.531815e-49	2.245480e-46
MsG0380014049.01	MsG0780040343.01	0.834428	3.015358e-56	3.577471e-53
MsG0380014049.01	MsG0780040700.01	0.809316	2.009477e-50	1.175995e-47
MsG0380014049.01	MsG0780041004.01	0.863016	3.363079e-64	1.004783e-60
MsG0380014049.01	MsG0780041262.01	0.837716	4.409857e-57	5.778197e-54
MsG0380014049.01	MsG0780041778.01	0.816874	4.414747e-52	3.165973e-49
MsG0380014049.01	MsG0880042343.01	0.817252	3.632169e-52	2.631588e-49
MsG0380014049.01	MsG0880044043.01	0.802721	4.905392e-49	2.420349e-46
MsG0380014049.01	MsG0880044105.01	0.800081	1.703367e-48	7.859840e-46
MsG0380014049.01	MsG0880044444.01	0.871941	4.655789e-67	1.912098e-63
MsG0380014049.01	MsG0880044487.01	0.819061	1.415356e-52	1.077851e-49
MsG0380014049.01	MsG0880044538.01	0.836152	1.106308e-56	1.382303e-53
MsG0380014049.01	MsG0880044558.01	0.807823	4.187582e-50	2.355851e-47
MsG0380014049.01	MsG0880046456.01	0.810011	1.425123e-50	8.495281e-48
MsG0380014049.01	MsG0880046723.01	0.800110	1.680674e-48	7.760677e-46
MsG0380014049.01	MsG0880046735.01	0.834067	3.713835e-56	4.358330e-53
MsG0380014049.01	MsG0880046736.01	0.812008	5.260901e-51	3.306304e-48
MsG0380014049.01	MsG0880046764.01	0.801256	9.809689e-49	4.663352e-46
MsG0380014049.01	MsG0880047460.01	-0.809407	1.921411e-50	1.127226e-47
MsG0280006848.01	MsG0380014049.01	0.802836	4.642793e-49	2.297630e-46
MsG0280008103.01	MsG0380014049.01	0.801270	9.745279e-49	4.634385e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014049.01.T01	MTR_4g051350	95.000	80	2	1	1	78	84	163	8.25e-45	144
MsG0380014049.01.T02	MTR_4g051350	95.652	46	2	0	1	46	118	163	8.65e-23	86.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014049.01.T01	ATCG00650	83.750	80	13	0	1	80	1	80	1.77e-41	132
MsG0380014049.01.T02	ATCG00650	85.417	48	7	0	1	48	33	80	2.66e-21	80.1

Find 7 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTTGTCCCCGATCCAATC+GGG	0.455483	3:-48730410	MsG0380014049.01.T01:CDS
GAGTTTGTCCCCGATCCAAT+CGG	0.465376	3:-48730411	MsG0380014049.01.T01:CDS
AAAGGTGACGAATTAACAAA+AGG	0.521079	3:+48730236	None:intergenic
GAGTCGCTTAGATTTATCCA+TGG	0.585856	3:+48730463	None:intergenic
TATCGACTTATTAGTCAACA+AGG	0.637963	3:-48730353	MsG0380014049.01.T01:CDS
GTTTGTCCCCGATCCAATCG+GGG	0.647747	3:-48730409	MsG0380014049.01.T01:CDS
TTTGTCCCCGATCCAATCGG+GGG	0.661952	3:-48730408	MsG0380014049.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTCATTATTAACAAA+AGG	+	Chr3:48730415-48730434	None:intergenic	10.0%
!!!	AATTAATCGTTGTTGTTTTA+AGG	+	Chr3:48730335-48730354	None:intergenic	20.0%
	AAAGGTGACGAATTAACAAA+AGG	+	Chr3:48730397-48730416	None:intergenic	30.0%
!	AATCTAAGCAATCTTTTCGT+AGG	-	Chr3:48730197-48730216	MsG0380014049.01.T01:CDS	30.0%
!!	TATCGACTTATTAGTCAACA+AGG	-	Chr3:48730277-48730296	MsG0380014049.01.T01:CDS	30.0%
	AATCAATTCGATCCCCCGAT+TGG	+	Chr3:48730237-48730256	None:intergenic	45.0%
	AGTTTGTCCCCGATCCAATC+GGG	-	Chr3:48730220-48730239	MsG0380014049.01.T01:CDS	50.0%
	ATTCGATCCCCCGATTGGAT+CGG	+	Chr3:48730232-48730251	None:intergenic	50.0%
	GAGTTTGTCCCCGATCCAAT+CGG	-	Chr3:48730219-48730238	MsG0380014049.01.T01:CDS	50.0%
	GTTTGTCCCCGATCCAATCG+GGG	-	Chr3:48730221-48730240	MsG0380014049.01.T01:CDS	55.0%
	TTCGATCCCCCGATTGGATC+GGG	+	Chr3:48730231-48730250	None:intergenic	55.0%
	TTTGTCCCCGATCCAATCGG+GGG	-	Chr3:48730222-48730241	MsG0380014049.01.T01:CDS	55.0%
	TCGATCCCCCGATTGGATCG+GGG	+	Chr3:48730230-48730249	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48730169	48730483	48730169	ID=MsG0380014049.01;Name=MsG0380014049.01
Chr3	mRNA	48730169	48730483	48730169	ID=MsG0380014049.01.T01;Parent=MsG0380014049.01;Name=MsG0380014049.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|104
Chr3	exon	48730169	48730483	48730169	ID=MsG0380014049.01.T01:exon:9379;Parent=MsG0380014049.01.T01
Chr3	CDS	48730169	48730483	48730169	ID=MsG0380014049.01.T01:cds;Parent=MsG0380014049.01.T01
Chr3	mRNA	48730169	48730387	48730169	ID=MsG0380014049.01.T02;Parent=MsG0380014049.01;Name=MsG0380014049.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|72
Chr3	exon	48730169	48730387	48730169	ID=MsG0380014049.01.T02:exon:9380;Parent=MsG0380014049.01.T02
Chr3	CDS	48730169	48730387	48730169	ID=MsG0380014049.01.T02:cds;Parent=MsG0380014049.01.T02
Chr3	mRNA	48730169	48730213	48730169	ID=MsG0380014049.01.T03;Parent=MsG0380014049.01;Name=MsG0380014049.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|14
Chr3	exon	48730169	48730213	48730169	ID=MsG0380014049.01.T03:exon:9381;Parent=MsG0380014049.01.T03
Chr3	CDS	48730169	48730213	48730169	ID=MsG0380014049.01.T03:cds;Parent=MsG0380014049.01.T03

Gene Sequence

>MsG0380014049.01.T01

ATGGATAAATCTAAGCGACTCTTTCTTAAATCTAAGCAATCTTTTCGTAGGAGTTTGTCCCCGATCCAATCGGGGGATCGAATTGATTATAAAAACATGAGTTTACTTTATCGACTTATTAGTCAACAAGGAAAAATATTATCTAGACGCGTGAATAGATCAACCTTAAAACAACAACGATTAATTACGATTGCTATAAAACAAGCTCGTATTTTATCTTTGTTACCTTTTGTTAATTCGTCACCTTTTGTTAATAATGAAAAAAAAAATATGAAAAAAGCGAGTCGATCACTAGAACTCCTACTACTGTTCTAA

>MsG0380014049.01.T03

ATGAAAAAAGCGAGTCGATCACTAGAACTCCTACTACTGTTCTAA

>MsG0380014049.01.T02

ATGAGTTTACTTTATCGACTTATTAGTCAACAAGGAAAAATATTATCTAGACGCGTGAATAGATCAACCTTAAAACAACAACGATTAATTACGATTGCTATAAAACAAGCTCGTATTTTATCTTTGTTACCTTTTGTTAATTCGTCACCTTTTGTTAATAATGAAAAAAAAAATATGAAAAAAGCGAGTCGATCACTAGAACTCCTACTACTGTTCTAA

Protein sequence

>MsG0380014049.01.T01

MDKSKRLFLKSKQSFRRSLSPIQSGDRIDYKNMSLLYRLISQQGKILSRRVNRSTLKQQRLITIAIKQARILSLLPFVNSSPFVNNEKKNMKKASRSLELLLLF*

>MsG0380014049.01.T03

MKKASRSLELLLLF*

>MsG0380014049.01.T02

MSLLYRLISQQGKILSRRVNRSTLKQQRLITIAIKQARILSLLPFVNSSPFVNNEKKNMKKASRSLELLLLF*